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inski
Cortinariologist



Registered: 02/28/06
Posts: 5,778
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: hamloaf]
#24836197 - 12/09/17 03:20 PM (6 years, 5 months ago) |
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hamloaf
Pork Block ®©™√



Registered: 12/23/09
Posts: 21,757
Loc: ation undisclosed.
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: inski]
#24836233 - 12/09/17 03:32 PM (6 years, 5 months ago) |
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Have you started the selfing process yet? I will be able to get back to this one in a few weeks. Going to go with RGS and WBS (cased). Might spawn a few to a manure based substrate.
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inski
Cortinariologist



Registered: 02/28/06
Posts: 5,778
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: hamloaf]
#24836253 - 12/09/17 03:42 PM (6 years, 5 months ago) |
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Hi Ham, I presume by the selfing process you mean to clone then print and fruit and repeat?
I haven't started that yet, I'm still in the first stages of deciding which of the six cultures is best, unfortunately it's getting quite warm now as we are coming into summer, I'll likely slant and store to resume the experiment when conditions become more favourable. I'm really keen to see how your experiment goes, cased grain will work well, give it a soak and drain after full colonisation and coir seems to work well as a casing for this species, gypsum is not needed.
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TheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,942
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: inski]
#24836295 - 12/09/17 04:05 PM (6 years, 5 months ago) |
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Does anyone know, potency wise, how these may compare to others? I did see that it is not very potent, but didn't know if it was on lvl of like cube or weaker.
-------------------- JOIN THE POW WOW
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inski
Cortinariologist



Registered: 02/28/06
Posts: 5,778
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: TheMadHatter420]
#24836321 - 12/09/17 04:17 PM (6 years, 5 months ago) |
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Quote:
TheMadHatter420 said: Does anyone know, potency wise, how these may compare to others? I did see that it is not very potent, but didn't know if it was on lvl of like cube or weaker.
I couldn't say from experience but this species has quite a strong blueing reaction to damage so I suspect it is reasonably potent, on the other hand, Heyowana who does have experience says wild specimens are not very potent and prefers P. papuana which is Potent.
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bobwastaken
under construction


Registered: 06/17/10
Posts: 1,987
Loc: SA
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: inski] 2
#24836819 - 12/09/17 10:16 PM (6 years, 5 months ago) |
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I guess I'm one of the few who has bioassayed this species so I'll chime in. I have had a mild trip from 10 grams fresh alutacea. Having experienced psilocybe subaeruginosa more frequently in the past I can say potency is less by weight. Never tried cubes sorry so can't draw any comparison there. Heyowana also finds P.Papuana to be stronger than P.Subs (which I consider extremely potent). I think he has a high tolerance compared to us mere mortals.
Not too far off my first attempt to fruit these. Narrowed down to 6 good looking sectors to test. Jars on the right were inoculated with wedges a week ago and should be done in a few days.
 These 2 were found in the wild a week ago after rain. Temps have been between 30 and 40c and cracker dry in the week leading up. We then had 2 days rain. Only the surface of the manure had been rehydrated so these specimens must have developed in around 48 hours.
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inski
Cortinariologist



Registered: 02/28/06
Posts: 5,778
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: bobwastaken]
#24836856 - 12/09/17 11:01 PM (6 years, 5 months ago) |
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Good work Bob, it will be great to compare experiments. If your cultures are clean it will be possible to fruit those directly from cased grain, break the spawn up and let recover over night then soak and drain the grain and lay out in a tray, case with coir.
Did you see any primordia form in your agar and what agar recipe did you use?
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bobwastaken
under construction


Registered: 06/17/10
Posts: 1,987
Loc: SA
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: inski]
#24836968 - 12/10/17 01:45 AM (6 years, 5 months ago) |
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I used 1/2 strength MEA but didn't see any primordia forming in any cultures after 3 transfers. Perhaps you have something special on your hands
Thanks for the advice on fruiting them I'll try to do as you have and post up the results.
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bobwastaken
under construction


Registered: 06/17/10
Posts: 1,987
Loc: SA
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: bobwastaken] 5
#24936838 - 01/23/18 05:29 PM (6 years, 3 months ago) |
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P.Alutacea pinning on colonized WBS cased with a 10mm layer 50/50 verm/coir
 Grow was done in a mini fridge I turned into a makeshift FC. It's far too hot to fruit them outdoors at the moment. I don't know the exact date when primordia formation began as I put these in the FC and went on a 12 day holiday. Pins were there when I returned home yesterday. Will take further pics as they develop. Also they were fruiting from the drainage holes under the potting containers. These were were lined with a dish cloth to prevent the WBS falling through. You can see the bruising on some specimens.

Edited by bobwastaken (01/23/18 10:25 PM)
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00Burnout
That one guy



Registered: 05/02/16
Posts: 2,186
Loc: Ozarks
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: bobwastaken]
#24936852 - 01/23/18 05:33 PM (6 years, 3 months ago) |
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-------------------- Peace, pot and microdot! No amount of progress has ever been made in the way of man without challenging the things we think we understand.-00Burnout Ghetto Greenhouse Trade List https://psychedelia.space
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pineninja
Dream Weaver



Registered: 08/17/14
Posts: 12,469
Loc: South
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: 00Burnout]
#24936909 - 01/23/18 05:51 PM (6 years, 3 months ago) |
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Holy cow....talk about on a roll.
How much do you like pizza.?
-------------------- Just a fool on the hill.
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bobwastaken
under construction


Registered: 06/17/10
Posts: 1,987
Loc: SA
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: pineninja] 1
#24937529 - 01/23/18 09:34 PM (6 years, 3 months ago) |
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Smothered in anchovies and I don't mind it. I take it you like mushrooms on yours
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AyePlus
Stony Danza



Registered: 12/18/14
Posts: 3,393
Loc: Fairfield, Connecticut
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: bobwastaken]
#24937621 - 01/23/18 10:22 PM (6 years, 3 months ago) |
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bobwastaken
under construction


Registered: 06/17/10
Posts: 1,987
Loc: SA
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: AyePlus] 4
#24951331 - 01/28/18 11:48 PM (6 years, 3 months ago) |
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Update of the P.Alutacea from straight WBS. I have some fully colonized Hpoo cakes ready to go in the FC so will be interesting to see how they compare to grains.



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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 3 months
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: bobwastaken]
#24951337 - 01/28/18 11:49 PM (6 years, 3 months ago) |
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--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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AyePlus
Stony Danza



Registered: 12/18/14
Posts: 3,393
Loc: Fairfield, Connecticut
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: cronicr]
#24951404 - 01/29/18 12:43 AM (6 years, 3 months ago) |
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Marvelous pics bob! Were those also done in the fridge FC?
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pineninja
Dream Weaver



Registered: 08/17/14
Posts: 12,469
Loc: South
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: AyePlus]
#24951409 - 01/29/18 12:50 AM (6 years, 3 months ago) |
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Wow. How did you go with the recent heatwave.
I also like anchovies.
-------------------- Just a fool on the hill.
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bobwastaken
under construction


Registered: 06/17/10
Posts: 1,987
Loc: SA
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: AyePlus]
#24951416 - 01/29/18 12:59 AM (6 years, 3 months ago) |
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Hey AyePlus, yes all are fruiting in the fridge FC. I took them outdoors to take pictures.
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bobwastaken
under construction


Registered: 06/17/10
Posts: 1,987
Loc: SA
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: pineninja]
#24951423 - 01/29/18 01:06 AM (6 years, 3 months ago) |
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Quote:
pineninja said: Wow. How did you go with the recent heatwave.
I also like anchovies.
Hey PN, I went well and so did the mushrooms as they live in a fridge. Your wood fired oven is
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pineninja
Dream Weaver



Registered: 08/17/14
Posts: 12,469
Loc: South
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: bobwastaken]
#24951453 - 01/29/18 02:02 AM (6 years, 3 months ago) |
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Nice place to be in 40 degree.
Thanks mate. Ill whip you up an anchovy special whenever your down my way.
-------------------- Just a fool on the hill.
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