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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: mistamonsta]
#24337012 - 05/21/17 12:02 AM (7 years, 9 hours ago) |
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send me a print and i will let you know
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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moricz
Inky Cap Cultivator



Registered: 07/14/13
Posts: 969
Loc: EU
Last seen: 7 months, 9 days
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: c10h12n2o]
#24358760 - 05/29/17 11:23 AM (6 years, 11 months ago) |
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I'm in
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catnip40
xฬ็



Registered: 03/09/12
Posts: 752
Last seen: 3 days, 10 hours
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: moricz]
#24358791 - 05/29/17 11:36 AM (6 years, 11 months ago) |
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saralove



Registered: 10/01/13
Posts: 1,068
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: bobwastaken]
#24372549 - 06/02/17 10:59 PM (6 years, 11 months ago) |
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Quote:
bobwastaken said:
Cheers saralove, I just photograph the sexy looking mushrooms. Setup is nothing fancy just an old Sony RX100 compact with the addition of ND filters and Raynox macroscopic lens. Can't recommend it though. By far the worst camera I've owned in terms of durability.
Cheers! Your photos come out beautiful either way. Love your Gym shots too. They are my favorite to photograph in the wild. Have a good season!

Quote:
inski said: Psilocybe tasmaniana is a species that is capable of fruiting from clay soil or very decomposed wood so it falls in between those true lignicolous species like P. subaeruginosa and P. cyanescens for example and the true terricolous species like P. aucklandiae or species from Section Mexicanae or Section Cordisporae, here is an image of P. tasmaniana fruiting from clay soil.

I have P. alutacea started on agar so can hopefully contribute to this thread soon, maybe it could fruit directly from cased grain.
Thanks for chiming in inski! I'm sure you'll cruise right thru the alus no problem.
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Listening to: emancipator - baralku tour (live) | AMU
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,619
Loc: Michigan
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: saralove]
#24372884 - 06/03/17 05:08 AM (6 years, 11 months ago) |
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-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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bobwastaken
under construction


Registered: 06/17/10
Posts: 1,987
Loc: SA
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: van hatton] 1
#24411617 - 06/16/17 11:58 PM (6 years, 10 months ago) |
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Been a bit quiet in here of late. I have some wild clones on agar at the moment. It was a modest challenge to get them to take due to contamination initially. Placing pins directly on agar failed in all attempts. I moved to tissue extraction. Being a diminutive species this can be tricky inside a glovebox. Procedure went as follows.
1. Sterilize all specimens prior to extraction by dunking in iodine. 2. Pluck the cap off to reveal the upper most concealed portion of stipe and transfer to agar.
Success rate has been greater than 90% with this method with 11/12 samples reverting within 24 hours. Slow but steady growth 4 days later without signs of contamination.
Using both MEA, H-POO agar (PH 6.6) with a first transfer to H-POO/aspirin agar (PH 5.5) to see if a slightly more acidic solution will improve growth rate. Will upload pics as things progress.
Edited by bobwastaken (06/17/17 01:08 AM)
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wildernessjunkie
Reshitivest


Registered: 06/13/10
Posts: 8,118
Loc: HTTP 404 Not Found
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: bobwastaken]
#24411741 - 06/17/17 01:45 AM (6 years, 10 months ago) |
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pineninja
Dream Weaver



Registered: 08/17/14
Posts: 12,469
Loc: South
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: bobwastaken]
#24413400 - 06/17/17 07:34 PM (6 years, 10 months ago) |
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In before oooohs and aahhhs.
-------------------- Just a fool on the hill.
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TheGrizzly
Stranger



Registered: 12/01/16
Posts: 56
Last seen: 3 years, 7 months
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: pineninja]
#24413443 - 06/17/17 07:53 PM (6 years, 10 months ago) |
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--------------------
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wildernessjunkie
Reshitivest


Registered: 06/13/10
Posts: 8,118
Loc: HTTP 404 Not Found
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: TheGrizzly]
#24413674 - 06/17/17 10:20 PM (6 years, 10 months ago) |
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If you wanted to get this species in circulation and into the hands of some competent folks you could easily do some spore swabs on those wild specimens. Then send them off to some experienced cultivators.
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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: wildernessjunkie]
#24413874 - 06/18/17 12:21 AM (6 years, 10 months ago) |
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thats a great idea i volunteer
id gladly do what i can with it, share my notes, and spread some prints when i fruit it
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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bobwastaken
under construction


Registered: 06/17/10
Posts: 1,987
Loc: SA
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: c10h12n2o]
#24413924 - 06/18/17 12:54 AM (6 years, 10 months ago) |
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roger that
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mistamonsta
Threadkiller



Registered: 07/06/11
Posts: 888
Loc: Uranus
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: bobwastaken] 1
#24415790 - 06/18/17 06:11 PM (6 years, 10 months ago) |
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Yes bobwastaken, I have been slack on this, hint taken I have been a bit distracted lately with band commitments and an upcoming job that will see me away from home for the next 3 months. I have collected plenty of data and wild observations of specimens and do have 10 dark prints of around 20mm diameter for circulation to those people interested in working towards cultivation and domestication of this species. I have just been too poor and too busy to get them out to people yet. This is a communal domestication and strain isolation project, as stated in my OP. THIS IS NOT A GIVEAWAY!!!!
with a brief message about why you want to work on this species and include any links/pics to your previous projects...... Hamloaf, EatY, Mushpunx, Bunya, you guys still interested???
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hamloaf
Pork Block ®©™√



Registered: 12/23/09
Posts: 21,757
Loc: ation undisclosed.
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: mistamonsta]
#24416719 - 06/19/17 02:15 AM (6 years, 10 months ago) |
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Bunya


Registered: 06/04/16
Posts: 601
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: hamloaf]
#24416940 - 06/19/17 05:48 AM (6 years, 10 months ago) |
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Cheers mate. Im all good. Have sent a few out.
-------------------- Bunya cones kill.
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ComebackKid
Multispore Enthusiast



Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: mistamonsta]
#24496637 - 07/20/17 03:58 PM (6 years, 9 months ago) |
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Thanks for linking HamLoaf
 Im not missing this
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Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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hamloaf
Pork Block ®©™√



Registered: 12/23/09
Posts: 21,757
Loc: ation undisclosed.
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: ComebackKid] 1
#24521790 - 07/31/17 10:45 PM (6 years, 9 months ago) |
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Germination
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bobwastaken
under construction


Registered: 06/17/10
Posts: 1,987
Loc: SA
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: hamloaf]
#24522039 - 08/01/17 04:42 AM (6 years, 9 months ago) |
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Great start hamloaf
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Steevo
Just a cog in the death wheel



Registered: 03/02/17
Posts: 2,702
Loc: Here and There
Last seen: 2 years, 1 month
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: bobwastaken]
#24522343 - 08/01/17 08:50 AM (6 years, 9 months ago) |
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I was lucky enough to get a print from saralove so I'll be following
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Germs
Space Force


Registered: 06/26/11
Posts: 4,607
Loc: Texas
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: Steevo]
#24522499 - 08/01/17 10:21 AM (6 years, 9 months ago) |
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Quote:
Steevo said: I was lucky enough to get a print from saralove so I'll be following
 Hopefully there are some of us that will attempt to fruit them!
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