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InvisibleGoldMold
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: bobwastaken]
    #25268856 - 06/14/18 09:25 PM (5 years, 10 months ago)

Bright yellow P.Alutacea Bob? That sounds sexy!

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InvisiblebobwastakenS
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: GoldMold] * 1
    #25288258 - 06/23/18 09:50 PM (5 years, 10 months ago)


Unfortunately haven't found them this season yet.

3rd flush on cased WBS. Still producing well. Fewer fruits but larger specimens.
I'll hang onto this dish to see how many flushes it will give.


Edited by bobwastaken (06/23/18 10:07 PM)

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Offlinepoisonshroom
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: bobwastaken]
    #25289881 - 06/24/18 08:03 PM (5 years, 10 months ago)

Awesome!

Mine arent doing all that well any more. My 2 T2 dishes have bacteria and/or yeast growing on them (and I didnt get time to make transfers when I first noticed, so now I think they are too far gone). One of my BRF jars triched up, but the other 2 are still moving along at snail-pace and I have another grain jar that is ready to do something with.

The grain jar I cased and put into fruiting conditions about a day after casing is coming along. It had a funny smell for the first few days in fruiting conditions (kind of fishy), but after I upped the FAE that went away. Its been in fruiting conditions for a bit over a week now I think, and I can see some mycelium poking through the casing layer (peat and vermiculite).

The jar I spawned to bulk is moving along, but very slowly. I kind of fucked that one up though - I went against my gut and used store bought cow manure, verm, coir and some gypsum. The cow manure is like mud, and I fucked the moisture levels up, so its too dry in some patches. Next time Ill try horse manure, roo poo or just coir (with verm and gypsum.

I might try a grain to agar transfer with the remaining grains before I spawn them to a better bulk sub.

We had a cold snap for a few days when I cased the first grain jar, where night time temps got down to about 7°c and days inly got up to about 26°c, so I have high hopes I might get some fruit from that lot.

:poison:

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InvisiblebobwastakenS
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: poisonshroom]
    #25291896 - 06/25/18 10:01 PM (5 years, 10 months ago)

Bugger about the contamination issues but then again some trial and error is part of the hobbie. Good to know you've got something to the fruiting stage. From my experience when cased and introduced to fruiting conditions it takes about a week (sometimes longer) once mycelium emerges through the casing to the formation of primordia. I'm very interested to see how things turn out for you.

I'm always impressed as to how much more robust this species grows when cultivated. For anyone that hasn't found this species in the wild it'd be easy to assume this is the norm but it's far from it.



I measured the pileus of a large indoor grown specimen today at 40mm.



By comparison 10-15mm is a good average for wild specimens with 20-25mm belonging to large specimens. The stipe from cultivated specimens is also much thicker than that of wild specimens.

Edited by bobwastaken (07/22/18 05:06 AM)

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Offlinepoisonshroom
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: bobwastaken]
    #25301951 - 07/01/18 01:32 AM (5 years, 10 months ago)

Its definitely very interesting the size difference between cultivated and wild. The opposite seems to be the case with a lot of other species. Panaeolus cyanescens for example tends to be teeny tiny in cultivation most of the time, whereas ones Iv found in the wild can be quite large (mostly tall, but the caps are usually still proportional).

My cased grains are still colonizing the casing, and are now poking out the top a fair bit, so Im hoping for some pins soon. The weather has been quite warm the last week or 2 though, so itll be interesting to see if it does anything.

Both of my bulk spawns seem to be contaminated with some kind of mold, so they are out, and I think only one of my BRF cakes will be any good. I still haven't done anything with my other grain jar, but I need to soon, because its looking like its drying out too much.

Im thinking Ill start the agar process from scratch again. I want to get an electric hotplate though, because my pressure cooker is small, and Im wasting gas by using the stove all the time.

:poison:

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Invisibleelasticaltiger
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: poisonshroom]
    #25301975 - 07/01/18 02:28 AM (5 years, 10 months ago)

God those look so fucking cool.

Sorry if it's been asked already but how is the trip?


--------------------
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InvisiblebobwastakenS
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: poisonshroom] * 3
    #25328824 - 07/15/18 10:19 PM (5 years, 9 months ago)

Quote:

poisonshroom said:
Its definitely very interesting the size difference between cultivated and wild. The opposite seems to be the case with a lot of other species. Panaeolus cyanescens for example tends to be teeny tiny in cultivation most of the time, whereas ones Iv found in the wild can be quite large (mostly tall, but the caps are usually still proportional).

My cased grains are still colonizing the casing, and are now poking out the top a fair bit, so Im hoping for some pins soon. The weather has been quite warm the last week or 2 though, so itll be interesting to see if it does anything.

Both of my bulk spawns seem to be contaminated with some kind of mold, so they are out, and I think only one of my BRF cakes will be any good. I still haven't done anything with my other grain jar, but I need to soon, because its looking like its drying out too much.

Im thinking Ill start the agar process from scratch again. I want to get an electric hotplate though, because my pressure cooker is small, and Im wasting gas by using the stove all the time.

:poison:




Yes that's right indoor pans do seem on the small side compared to wild. P.alutacea take well to indoor cultivation on that front.

Have you seen any pins since the last post or is the weather against you?
Hope you see some reward for your efforts.

Elasticaltiger, I've only tried a small dose. They give a very euphoric trip from what I gathered but I'm waiting on an experienced tripper to give a report on potency and overall experience.

This is the 4th flush coming to maturity.

Edited by bobwastaken (07/15/18 11:37 PM)

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Offlinepoisonshroom
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: bobwastaken]
    #25339973 - 07/22/18 02:37 AM (5 years, 9 months ago)

No pins yet unfortunately. The ones I spawned to manure all turned green (as half-expected), but the cased grains are still just going along not really doing anything. More and more mycelium is poking through the casing, and it looks like there may be some signs its going to pin soon, but hard to tell.

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InvisiblebobwastakenS
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: poisonshroom]
    #25339995 - 07/22/18 03:44 AM (5 years, 9 months ago)

Looking good poisonshroom:thumbup: Are the temps still favourable?

I'll be going grains to spawn bags of manure next time round myself. The high ratio of grain spawn required for timely colonization of bulk substrate isn't worth it IMO.

Edited by bobwastaken (07/22/18 04:30 AM)

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Offlinepoisonshroom
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: bobwastaken]
    #25340007 - 07/22/18 04:16 AM (5 years, 9 months ago)

We've gone back into a bit of a warm period unfortunately. It was supposed to be colder this weekend, but ended up being fairly warm (Im not even wearing a jumper at the moment, and Im allergic to cold).

I think the warm weather has allowed it to colonize into the casing a bit better though, but Im not sure if thats a good thing or not. For now Im just misting every day and keeping it in a box with the lid partially open with the fan on in the room. I might stick it in the fridge for a week or so soon though if it continues to do nothing and see if a proper cold shock helps.

It would interesting to see how big they get in a spawn bag.

Given how old and crusty the manure they grow on in the wild looks in pictures (moss must surely take a year or 2 at least to grow like that), I wonder how they would go in compost beds or pot plants with plenty of compost? Maybe some spawn could be planted and allowed to grow over summer and it will fruit naturally over winter. Then when it warms up again, give it some more compost to keep it going.

:poison:

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InvisiblebobwastakenS
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: poisonshroom]
    #25348662 - 07/26/18 06:13 PM (5 years, 9 months ago)

It's a good thing to have the casing colonize. I have had a container fruit early due to contamination which isn't what you want. Healthy spawn without the presence of metabolites will fruit when the casing has been colonized IME. Thanks again for the updates.

Edited by bobwastaken (07/26/18 06:19 PM)

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Offlinepoisonshroom
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: bobwastaken]
    #25382854 - 08/12/18 04:14 AM (5 years, 9 months ago)

So, time for an update...

Nothing was happening, so I decided to cover it in gladwrap and put it in the fridge for a while to try and cold shock it. I just dont think its cold enough to initiate fruiting in natural conditions here.

Its been in there since last thursday, and  Im going to take it out now and try again (to be honest the only reason I didnt take it out sooner is because I got side-tracked and forgot about it).

Its apparently going to drop into single digit overnight temperatures for a few days early in the week, but summer is definitely on its way back and its starting to get hot.

:poison:

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InvisiblebobwastakenS
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: poisonshroom]
    #25397728 - 08/19/18 04:36 AM (5 years, 8 months ago)

Bugger about the weather. I've seen hyphal knots form on agar plates kept in the fridge for a month. Thanks for the update and good luck.

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InvisibleGerms
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: bobwastaken] * 2
    #25417812 - 08/27/18 08:36 PM (5 years, 8 months ago)


Took awhile in this warm weather, but I have this quart of wheat colonized and another not far behind
Make a tub of cased grain, or mix with bulk substrate?


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InvisiblebobwastakenS
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: Germs] * 1
    #25418103 - 08/27/18 10:27 PM (5 years, 8 months ago)

They're looking great germs. As you know they're not a fast colonizer as cubensis so if you do go to bulk make sure you use a high spawn ratio or you may see contamination. The species colonizes much faster when frequently agitated. I prefer to do the spawn run in jars or for larger grows spawn bags and shake frequently. They'll fruit prolifically from straight cased grain or manure. From a little over a quart of cased grain I've seen around 500 fruits.

Edited by bobwastaken (08/27/18 10:31 PM)

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InvisiblebobwastakenS
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: bobwastaken] * 6
    #25590453 - 11/03/18 11:40 PM (5 years, 6 months ago)

An update on the latest grow with P.alutacea. I collected a small specimen from the grampians recently. These containers are multispore and substrate is horse manure. Knots began to form around 10 days after full colonization with temps kept at 14-15c. I have some other colonized containers sitting around at 20c and while I've noticed they will produce hyphal knots at this temp it appears they don't develop into mushrooms. Also removing fruiting containers from the FC into a warmer environment causes significant oxidation.


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Invisiblepineninja
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: bobwastaken]
    #25597140 - 11/06/18 08:03 PM (5 years, 6 months ago)

Holy Balls.:rockon:


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InvisibleBunya
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: pineninja]
    #25598678 - 11/07/18 01:48 PM (5 years, 6 months ago)

Wow you have definitely cracked the code


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OfflineAsuraS
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: Bunya]
    #25599437 - 11/07/18 07:08 PM (5 years, 6 months ago)

That is glorious.

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InvisiblebobwastakenS
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: Asura] * 4
    #25599906 - 11/08/18 01:30 AM (5 years, 6 months ago)

:cheers:
Some more P.alutacea porn.
These are also growing from manure cased with a layer of moss. A much more robust morphology.







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