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poisonshroom



Registered: 12/02/07
Posts: 323
Loc: Northern Aus
Last seen: 1 month, 15 days
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: bobwastaken]
#25252932 - 06/06/18 08:02 PM (5 years, 11 months ago) |
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So I bought a reptile heating mat to see if that speeds things up a bit. Its pretty pathetic though - I tested it by leaving it on for an hour or so and it was only slightly warm. I have my jars and plates in a plastic shoebox with the hest mat underneath for now, and it seemed as if it was slightly warmer than the room, so I guess Ill have to see.
Seeing as you say shaking every few days is necessary for them to go faster than snail pace, I have a feeling the BRF jars will take forever. The mycelium looks quite strong and healthy though - looks a bit like cubensis mycelium, but not ropey, just thick and white.
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bobwastaken
under construction


Registered: 06/17/10
Posts: 1,987
Loc: SA
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: poisonshroom]
#25252960 - 06/06/18 08:14 PM (5 years, 11 months ago) |
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I also have to incubate my jars at the moment. I bought one of these cheap mats /thermostat bundle and the 14W is enough to maintain 20-25c in a room which is around 8-10c
 I use a broken 200l fridge. Mat is at the bottom and set the stat to 25. I guess you could also try an esky or styrofoam box for additional insulation.
Sounds like you have some healthy P.Alutacea mycelium I find it won't grow rhizomorphic on grains but will do so on manure.
Edited by bobwastaken (06/06/18 08:15 PM)
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AgentGFunk
Boss DJ



Registered: 01/01/15
Posts: 290
Loc: Zion
Last seen: 2 months, 23 days
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: bobwastaken]
#25253130 - 06/06/18 10:01 PM (5 years, 11 months ago) |
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Noooo you wanna build an INCUBATOR.
I'll post a link in a minute.
-------------------- The voyage to the corner of the globe is a real TRIP.

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AgentGFunk
Boss DJ



Registered: 01/01/15
Posts: 290
Loc: Zion
Last seen: 2 months, 23 days
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: AgentGFunk]
#25253142 - 06/06/18 10:10 PM (5 years, 11 months ago) |
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Andddd never mind I can't find it anymore. Damn that was such a good thread. Dude makes an incubator out of two totes. Fills the bottom of one with water about 1/10 of the way full, puts a special heater in it that has a thermometer attached to it that switches the heater on and off. Thermometer goes in the top tote. Water heats up as needed to get it up to temp when it drops below a temp set by the user. Lid goes on the top tote. Holes drilled on the small top lips of each tote and nylon rope ties them together tightly. It's quite clever. I can't remember who made that thread. Maybe cronicr? Maybe Munchauzen? Can't remember......
-------------------- The voyage to the corner of the globe is a real TRIP.

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poisonshroom



Registered: 12/02/07
Posts: 323
Loc: Northern Aus
Last seen: 1 month, 15 days
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: AgentGFunk]
#25253323 - 06/07/18 01:27 AM (5 years, 11 months ago) |
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Sounds about right. Id say the heater the guy you are talking about is a fishtank heater. I have a few of them around, but I may need them for the fish.
My heat mat is just a little 5watt, so Id say styrofoam will probably be better. Thats what you would call an incubator too, just not very insulated, being thin plastic with only a weak heat source.
By the way AgentGFunk - is that a Squier Jagmaster in your sig?
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bobwastaken
under construction


Registered: 06/17/10
Posts: 1,987
Loc: SA
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: poisonshroom]
#25253449 - 06/07/18 04:36 AM (5 years, 11 months ago) |
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I have an incubator..What you're talking about sounds like the tub in tub incubator design I've come across. They're crap unless you seal the aquarium heater separately in an airtight container. If not the incubation space becomes very humid and in no time it's full of molds. The last thing you want is high humidity in a warm space filled with your substrate jars.
Edited by bobwastaken (06/07/18 04:40 AM)
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poisonshroom



Registered: 12/02/07
Posts: 323
Loc: Northern Aus
Last seen: 1 month, 15 days
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: bobwastaken] 1
#25258717 - 06/10/18 12:10 AM (5 years, 11 months ago) |
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The heat mat and shaking seems to be doing the trick. Things have sped up dramatically, even though it seems to be causing some funky moisture issues - lots of condensation in the grain jars, and the condensation on the lid of the agar plate thats in there is now forming bigger droplets, which makes viewing easier, but a couple have dripped onto the mycelium.
The BRF jars are speeding ahead now too. I think Ill make some transfers from the plate in the next few days (there is a couple of nice sectors) and use those T2 plates for spawn, so I still have a few different colonies to increase the chance of getting a good fruiting colony. Then if i get fruit, Ill take a clone and/or print of the best one(s) and go from there.
I may also make some slants for cold-storage from the T2 plates or the T1 plate too for use as masters.
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poisonshroom



Registered: 12/02/07
Posts: 323
Loc: Northern Aus
Last seen: 1 month, 15 days
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: poisonshroom]
#25264889 - 06/13/18 03:25 AM (5 years, 11 months ago) |
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Made my T2 transfers onto 3 plates last night, but I kind of botched it because I couldnt really see what I was doing There is already bacteria growing on the T1 plate.
On the bright side, it looks like 2 of my grain jars are ready to spawn. What do you guys think?

The next thing is substrate: I have some store bought composted manure I could use, or I could either collect some roo poo or buy some horse manure (my parents live on the outskirts of town and there are often people who own horses selling bags of it on the side of the road when I go out there, so its easy to obtain, but can be a bit too fresh). Which do you think would be best? Iv had success with other dung-lovers on the store bought stuff (just from bunnings), but it does tend to contain more than just manure despite what the bag says (looks like sugar cane mulch or something mixed in).
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Warrk



Registered: 06/02/17
Posts: 1,662
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: poisonshroom]
#25264929 - 06/13/18 04:12 AM (5 years, 11 months ago) |
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The grain jars look great!
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bobwastaken
under construction


Registered: 06/17/10
Posts: 1,987
Loc: SA
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: Warrk]
#25264980 - 06/13/18 05:41 AM (5 years, 11 months ago) |
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As Warrk said they look great. You really have a few options now. I would leave the jars another few days to make sure they're done. It appears your grains may be on the dry side. I've found if I make them on the dry side the mycelium will not colonize the grains as completely.
They will fruit well from straight cased grains if you wanted to go that route. I will say I haven't had any success spawning to bulk with this species. The colonization time being slower than cubensis threw me off and my bulk substrate saw only partial colonization before contaminating after 2 weeks. I suggest you try a much higher ratio of 2:1 or so. You'll want well aged manure of either kind. If you can collect kangaroo or horse manure from paddocks that'd be perfect. It should smell earthy.
Do you have back up jars incase things don't turn out? If not perhaps you could do some grain to grain transfers. They will colonize very fast g2g.
Thanks again for documenting your grow. Looking forward to any updates.
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poisonshroom



Registered: 12/02/07
Posts: 323
Loc: Northern Aus
Last seen: 1 month, 15 days
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: bobwastaken]
#25266575 - 06/13/18 07:40 PM (5 years, 11 months ago) |
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Thanks. Id say they are a bit dry - this is the first time Iv got grains to actually work - moisture levels being the main culprit usually.
I have one more jar which isnt quite there yet, so Ill probably use these over the weekend to try a couple of things and have the other as a backup and to use for some g2g transfers.
They are only half-pints, so I dont have a huge amount to work with, but Im not particularly interested in eating any at the moment anyway, so I dont mind small yields (if I even get any fruit).
Ill probably use one for just straight cased grains, and the other to spawn to either manure or coir/verm/gypsum. And for at least one of them Ill split it in half and cold shock one in the fridge before outting it into fruiting conditions and the other Ill just go straight to fruiting once its ready and see what happens.
Its warmed up again this week, so our night time temperatures are back into the teens, but thats usually how it goes up here in winter - cycles of warmish and cold nights for a week or 2 at a time.

Ps im going back through this thread and Inski's grow to get as much info as I can to figure out the best plan of attack. Plus I just love seeing those caramel/toffee coloured caps
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GoldMold
Stranger in the night


Registered: 10/13/16
Posts: 737
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: poisonshroom]
#25266666 - 06/13/18 08:36 PM (5 years, 11 months ago) |
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Hey guys and gals, I love following this thread these fruits are so beautiful. Does anyone know a vendor that sells these prints? If not I was wondering if anyone here would have one to trade. I have plenty of different cube prints and some nice ATL#7 prints I could trade also.
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bobwastaken
under construction


Registered: 06/17/10
Posts: 1,987
Loc: SA
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: GoldMold]
#25266742 - 06/13/18 09:17 PM (5 years, 11 months ago) |
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A great idea not placing all eggs in the one basket.
This latest P.Alutacea grow from cased grains.

Edited by bobwastaken (06/13/18 09:18 PM)
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Asura
Cyantist


Registered: 08/01/11
Posts: 5,082
Loc: Right Here
Last seen: 39 minutes, 11 seconds
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: bobwastaken]
#25266785 - 06/13/18 09:34 PM (5 years, 11 months ago) |
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Bob, your killing it. I'm not putting it to agar until November (in the States and all) and going to do an outdoor bed. I'm hoping this isn't like azures where I have to wait 10 months for fruits.
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GoldMold
Stranger in the night


Registered: 10/13/16
Posts: 737
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: Asura]
#25266788 - 06/13/18 09:36 PM (5 years, 11 months ago) |
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Got damn Bob them are perfect!
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bobwastaken
under construction


Registered: 06/17/10
Posts: 1,987
Loc: SA
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: GoldMold]
#25266807 - 06/13/18 09:52 PM (5 years, 11 months ago) |
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Thanks guys. Asura, there's no 10 month wait for these
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poisonshroom



Registered: 12/02/07
Posts: 323
Loc: Northern Aus
Last seen: 1 month, 15 days
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: bobwastaken]
#25266876 - 06/13/18 10:41 PM (5 years, 11 months ago) |
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Wow! Those look great!
I love the reddish colour the south australian ones seem to have. How big are those? Its hard go gauge scale from most of the pictures even with the pyrex dish with measurements on it.
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Warrk



Registered: 06/02/17
Posts: 1,662
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: bobwastaken]
#25267136 - 06/14/18 03:54 AM (5 years, 11 months ago) |
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Quote:
bobwastaken said:
This latest P.Alutacea grow from cased grains.
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elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
Posts: 8,438
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: Warrk]
#25267248 - 06/14/18 06:14 AM (5 years, 11 months ago) |
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That's fucking beautiful mang.
-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
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bobwastaken
under construction


Registered: 06/17/10
Posts: 1,987
Loc: SA
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Re: Potential for Domestication and strain Isolation of Ps. Alutaceae [Re: elasticaltiger]
#25268818 - 06/14/18 09:06 PM (5 years, 10 months ago) |
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Cheers all.
PoisonShroom, they do tend to be more red/orange compared to finds I see from Victoria. There is also a bright yellow phenotype of P.Alutacea in SA which I'd like to cultivate at some point.
They're a small species and indoors they'll grow from around 80-100mm tall at full maturity. The pileus is typically larger on indoor specimens varying from 10-35mm in diameter. Wild specimens on average are smaller all round and don't bruise as readily or intensely.
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