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Th3Issu3
Stranger Danger


Registered: 12/30/14
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Loc: fucktown
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Re: Looking for tips on agar transfers in terms of technique [Re: Intelligentxfruit] 2
#23966000 - 12/29/16 04:19 PM (7 years, 2 months ago) |
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When transferring a agar wedge face up.
When fucking dem bitched face down!
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Tiamo
Trust in LITFA




Registered: 04/07/16
Posts: 1,935
Loc: Amsterdam
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Re: Looking for tips on agar transfers in terms of technique [Re: enlightenment]
#23966005 - 12/29/16 04:21 PM (7 years, 2 months ago) |
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Quote:
enlightenment said: It looks like you cut from a strange angle. Try to cut from 60-90° position (scalpel).
It's bad practice to let your hand go above the plate right? That's why I've been having so much trouble doing transfers. My hand gets all weird and I'm scared to move it any way really.
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ComebackKid
Multispore Enthusiast



Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
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Re: Looking for tips on agar transfers in terms of technique [Re: Intelligentxfruit]
#23966006 - 12/29/16 04:22 PM (7 years, 2 months ago) |
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Take a shot of vodka or whisky before SAB work!
I like to use both hands when making a transfer. I'll hold the plate up in one hand and tip it toward my scalpel while making my cuts. Feels more natural than trying to work with it flat on the table.
I like to cut little skinny oval slivers out if the leading edge. Small as possible. I'll make two cuts starting from the same spot and ending in the same spot. Before I pull the scalpel out of the agar after my second slice, I put pressure with the side of my scalpel and squeeze my wedge out of the agar and balance it on the flat of my scalpel. Now I have a tiny agar flake laying on my scalpel blade which then I'll carefully move to the center of my next plate. I'll then run my blade through the next plate leaving the agar wedge behind.
You can do it with squares or triangles as well just make sure after your final slice that you leave the blade in and put pressure on your wedge to pop it out and rest it on your blade 
I used to have trouble with wedges sticking to my scalpel from squewering them. I came up with this method after many frustrating transfers. You'll find what works for you after working with it long enough. It's a pretty forgiving practice tbh. If you fuck it up now you'll be able to fix it in a few days after it grows out again
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Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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Tookitooki
Mycological Fabricator



Registered: 07/28/16
Posts: 1,157
Loc: Nowhere
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Re: Looking for tips on agar transfers in terms of technique [Re: ComebackKid]
#23966069 - 12/29/16 04:56 PM (7 years, 2 months ago) |
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I recently had the same issue as the OP, spent most of yesterday and today searching for a better way. I resorted to scraping the myc off the agar. I guess I didn't pay much attetion to the fact they were pulling a chunk of agar up with it.
Now I'm in search of a good option for making liquid inoculate, with out sacrificing my whole agar dish.
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Dabrit



Registered: 12/02/15
Posts: 523
Loc: Pacific Northwest
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Re: Looking for tips on agar transfers in terms of technique [Re: ComebackKid]
#23966078 - 12/29/16 05:02 PM (7 years, 2 months ago) |
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Quote:
ComebackKid said: Take a shot of vodka or whisky before SAB work!
I like to use both hands when making a transfer. I'll hold the plate up in one hand and tip it toward my scalpel while making my cuts. Feels more natural than trying to work with it flat on the table.
I like to cut little skinny oval slivers out if the leading edge. Small as possible. I'll make two cuts starting from the same spot and ending in the same spot. Before I pull the scalpel out of the agar after my second slice, I put pressure with the side of my scalpel and squeeze my wedge out of the agar and balance it on the flat of my scalpel. Now I have a tiny agar flake laying on my scalpel blade which then I'll carefully move to the center of my next plate. I'll then run my blade through the next plate leaving the agar wedge behind.
You can do it with squares or triangles as well just make sure after your final slice that you leave the blade in and put pressure on your wedge to pop it out and rest it on your blade 
I used to have trouble with wedges sticking to my scalpel from squewering them. I came up with this method after many frustrating transfers. You'll find what works for you after working with it long enough. It's a pretty forgiving practice tbh. If you fuck it up now you'll be able to fix it in a few days after it grows out again 
Solid advice on technique old chap thanks. Unfortunately I'm taking a break from booze. But next time I'll transfer sans café
-------------------- Psychedelics = True gateway to spiritual enlightenment and detachment from ones meat costume
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wild child
Stranger
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Re: Looking for tips on agar transfers in terms of technique [Re: Dabrit]
#23966196 - 12/29/16 05:57 PM (7 years, 2 months ago) |
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ive yet to start Agar, however my question is...when going to put a wedge into a grain jar..is it okay to have agar on it or will that help contam the jar...basically should it be 100 myc..added or can there be some agar stuck to it?
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ComebackKid
Multispore Enthusiast



Registered: 05/27/16
Posts: 3,951
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Re: Looking for tips on agar transfers in terms of technique [Re: wild child]
#23966208 - 12/29/16 06:01 PM (7 years, 2 months ago) |
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"The tiger drop" is a method where one will drop a whole clean agar culture into a jar of grains. Agar wedges will do fine too though 
Edit: visuals
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Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
Edited by ComebackKid (12/29/16 06:04 PM)
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wild child
Stranger
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Re: Looking for tips on agar transfers in terms of technique [Re: ComebackKid]
#23966211 - 12/29/16 06:04 PM (7 years, 2 months ago) |
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gotcha thanks, btw...Ive been doing your water bottom tek and its working great.
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ComebackKid
Multispore Enthusiast



Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
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Re: Looking for tips on agar transfers in terms of technique [Re: wild child]
#23966241 - 12/29/16 06:18 PM (7 years, 2 months ago) |
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Good to hear man! I'm glad people are still able to find that tek.
Hey Darbit! Chamomile tea is known to calm the nerves a bit. Might be a good alternative to the cafe and alcohol? Good luck with your break man
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Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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Dabrit



Registered: 12/02/15
Posts: 523
Loc: Pacific Northwest
Last seen: 7 months, 29 days
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Re: Looking for tips on agar transfers in terms of technique [Re: ComebackKid]
#23966255 - 12/29/16 06:24 PM (7 years, 2 months ago) |
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Quote:
ComebackKid said: Good to hear man! I'm glad people are still able to find that tek.
Hey Darbit! Chamomile tea is known to calm the nerves a bit. Might be a good alternative to the cafe and alcohol? Good luck with your break man
Thanks man, yeah I think I do have some sleepytime tea kicking around somewhere which is basically just regular old chamomile. I'll pop on the kettle when I'm ready to transfer to grains.
-------------------- Psychedelics = True gateway to spiritual enlightenment and detachment from ones meat costume
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Kenetic
Nam Sayin



Registered: 08/24/14
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Re: Looking for tips on agar transfers in terms of technique [Re: Dabrit] 1
#23966304 - 12/29/16 06:48 PM (7 years, 2 months ago) |
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Try taking some kratom before you do any agar work. It will relax the fuck out of you and before you know it you'll be doing agar in your sleep.
My first time, I had a shirt tied around my face and was sweating balls and being clumsy, breathing hard and slightly shaking too! I spilled agar on the sides of the dishes and was just all-around sloppy. Now I just wear gloves and wipe down with iso and I'm good to go. My heart-rate doesn't even change, I'm completely relaxed and confident. The key is to stay current and practice. At one point I was doing about 50 plates a week and it was just routine then. I did other stuff too like make blenderless li and noc up grains with agar or li. Practice makes perfect
Ok recess is over kids.
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mushroom_therapy
Apprentice



Registered: 03/23/17
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Re: Looking for tips on agar transfers in terms of technique [Re: Kenetic]
#24271655 - 04/25/17 08:25 PM (6 years, 10 months ago) |
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50 a week??! Where do you get your Petri dishes from and how much do you pay?!
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Munchauzen


Registered: 06/22/11
Posts: 14,343
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Re: Looking for tips on agar transfers in terms of technique [Re: mushroom_therapy] 1
#24271669 - 04/25/17 08:36 PM (6 years, 10 months ago) |
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check out sponsors I'm sure there is some good deals on sleaves of 100-500 dishes
or just go all out and buy 100 glad mini rounds. with transfers, i usually keep them around 2 weeks, so 100 will allow you to do 50 a week. at 50 cents a pop, thats $50 for more pasty plates than you will ever want to look at
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mushroom_therapy
Apprentice



Registered: 03/23/17
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Re: Looking for tips on agar transfers in terms of technique [Re: Munchauzen]
#24271723 - 04/25/17 09:02 PM (6 years, 10 months ago) |
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Thanks for the reply! Ive only used the glad minis but I have a hard time being able to see the mycellium clearly through these. I will check out sponsor sites, thanks!
-------------------- Speak to me in energy, that way I can understand you better.
ALSO...check out this fellow shroomites dope tunes... LOBIS' soundcloud playlist
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Dabrit



Registered: 12/02/15
Posts: 523
Loc: Pacific Northwest
Last seen: 7 months, 29 days
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Re: Looking for tips on agar transfers in terms of technique [Re: mushroom_therapy]
#24310563 - 05/10/17 06:41 PM (6 years, 9 months ago) |
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I'm thinking that pasty plates are the way to go now over regular Petris. It's just too much of a pain in the ass trying to pour agar inside of a SAB.
I'll wait until I move up in the world and have an actual HEPA Laminar flow hood.
-------------------- Psychedelics = True gateway to spiritual enlightenment and detachment from ones meat costume
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jkz
Padawan



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Posts: 1,910
Loc: Coruscant
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Re: Looking for tips on agar transfers in terms of technique [Re: Dabrit]
#24310673 - 05/10/17 07:47 PM (6 years, 9 months ago) |
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What do you do if you don't have a scapel lol
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jeezy117
FunGuy



Registered: 05/31/09
Posts: 100
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Re: Looking for tips on agar transfers in terms of technique [Re: jkz]
#24310694 - 05/10/17 07:55 PM (6 years, 9 months ago) |
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Thoroughly interested in this myself.
When you say 90 degrees do you mean holding the petri dish in your left hand at a 90 and cutting with your right?
I thought that the goal was to never have your hands hover over anything in a SAB?
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Munchauzen


Registered: 06/22/11
Posts: 14,343
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Re: Looking for tips on agar transfers in terms of technique [Re: jeezy117]
#24310698 - 05/10/17 07:58 PM (6 years, 9 months ago) |
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Quote:
jeezy117 said: Thoroughly interested in this myself.
When you say 90 degrees do you mean holding the petri dish in your left hand at a 90 and cutting with your right?
I thought that the goal was to never have your hands hover over anything in a SAB?
why use words when I can just show you
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jeezy117
FunGuy



Registered: 05/31/09
Posts: 100
Last seen: 5 years, 9 months
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Re: Looking for tips on agar transfers in terms of technique [Re: Munchauzen]
#24312506 - 05/11/17 05:20 PM (6 years, 9 months ago) |
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Quote:
Munchauzen said:
Quote:
jeezy117 said: Thoroughly interested in this myself.
When you say 90 degrees do you mean holding the petri dish in your left hand at a 90 and cutting with your right?
I thought that the goal was to never have your hands hover over anything in a SAB?
why use words when I can just show you
Way to go and making it look simple.
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