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 bodhisatta  
Smurf real estate agent
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
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 BOD's DIY Inoculation Loop TEK   13
#24292526 - 05/03/17 01:47 PM (6 years, 11 months ago) |
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This tek is part of bod's cultivation is easy as fuck link listBod's DIY Inoculation Loop TEK
Using X-Acto style handles and nichrome wire or easily found as vape coil wire  You Will Need Some wire, experiment with different lengths 3.5-4 inches works well for me. some sort of thin rod to make the loop with, I used a thermometer and a handle, I used the x-acto for the clamping mechanism. This is how we doTake the x-acto knife apart fold a wire over to make a U shape and put the pointy ends into the blade receiver of the x-acto handle   now you have one of these    put your thin rod thing through, then push the wire so that it forms a loop around the utensil before you start to twist   if you don't do that  ugly mess if you spend the time to smooth it out    I also buy sterile disposable ones with much smaller loops 1 microliter(uL) they will both work for streaking spores out. With these ones you can bend your loop's eye to make it smaller to hold onto a drop of solution. for example take this then crimp it into  this     Flame away
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hamloaf
Pork Block
Registered: 12/23/09
Posts: 21,422
Loc: ation is turned off.
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 Re: BOD's DIY Inoculation Loop TEK [Re: bodhisatta]
#24292533 - 05/03/17 01:49 PM (6 years, 11 months ago) |
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Nice one. 
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 Organic_Magic
Medicine Man
Registered: 10/26/14
Posts: 1,712
Loc: Everywhere
Last seen: 2 years, 8 months
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Re: BOD's DIY Inoculation Loop TEK [Re: bodhisatta]
#24292541 - 05/03/17 01:51 PM (6 years, 11 months ago) |
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This is EXACTLY how I do it. People always forget the versatility of an x-acto handle.
Good stuff bod. 
--------------------
 Its all fun and games until mushrooms make you gay
  
click and learn.
Bod's simplified cultivation methods
Got side pins?
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mushboy
modboy
Registered: 04/24/05
Posts: 32,776
Loc: where?
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Re: BOD's DIY Inoculation Loop TEK [Re: hamloaf]
#24292545 - 05/03/17 01:53 PM (6 years, 11 months ago) |
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tight
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mynakedrat
The phantom hourglass
Registered: 02/16/17
Posts: 4,629
Loc: Inner Astral levels 
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Re: BOD's DIY Inoculation Loop TEK [Re: mushboy] 1
#24292551 - 05/03/17 01:55 PM (6 years, 11 months ago) |
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I do this too!
I use a bread tie, remove the paper /plastic edges.
Awesome once again sir! 
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SuperFly
Still in the Space Race
Registered: 05/19/13
Posts: 1,032
Loc: Dark side off the moon
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Re: BOD's DIY Inoculation Loop TEK [Re: mynakedrat]
#24292579 - 05/03/17 02:05 PM (6 years, 11 months ago) |
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Quote:
mynakedrat said:
I do this too!
I use a bread tie, remove the paper /plastic edges.
Awesome once again sir!
I do the same thing lol. I was thinking this looks so much nicer than my garbage twisty-tie.
Bod is swiping two prints at one time... Now that's gangster 
Edited by SuperFly (05/03/17 02:18 PM)
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mynakedrat
The phantom hourglass
Registered: 02/16/17
Posts: 4,629
Loc: Inner Astral levels
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Re: BOD's DIY Inoculation Loop TEK [Re: SuperFly]
#24292583 - 05/03/17 02:07 PM (6 years, 11 months ago) |
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Can contams get in the twists in the loop though?
Do you always flame the entire thing?
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hamloaf
Pork Block
Registered: 12/23/09
Posts: 21,422
Loc: ation is turned off.
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Re: BOD's DIY Inoculation Loop TEK [Re: mynakedrat]
#24292589 - 05/03/17 02:09 PM (6 years, 11 months ago) |
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I always flame the entire wire.
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bodhisatta
Smurf real estate agent
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
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Re: BOD's DIY Inoculation Loop TEK [Re: mynakedrat]
#24292595 - 05/03/17 02:14 PM (6 years, 11 months ago) |
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Quote:
mynakedrat said:
Can contams get in the twists in the loop though?
Do you always flame the entire thing?
in my agar tek I talk about how I keep a jar of 91% iso like a baby food jar. I dip my tools in there first and then flame them red hot. this way flame gets into all the crevices on tools.
This is how a glass spreading rod would be sterilized since you can't hold glass in a flame till it's red hot and then touch a petri dish, it won't cool down fast enough. And it will eventually shatter too.
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Munchauzen
Registered: 06/22/11
Posts: 14,344
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Re: BOD's DIY Inoculation Loop TEK [Re: bodhisatta]
#24292742 - 05/03/17 03:25 PM (6 years, 11 months ago) |
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Im sure you meant to say 70%, not 91%.
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bodhisatta
Smurf real estate agent
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
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Re: BOD's DIY Inoculation Loop TEK [Re: Munchauzen]
#24292746 - 05/03/17 03:26 PM (6 years, 11 months ago) |
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Nope 91 is more flammable burns hotter. And i would imagine has lower surface tension to get in more places
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Munchauzen
Registered: 06/22/11
Posts: 14,344
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Re: BOD's DIY Inoculation Loop TEK [Re: bodhisatta] 2
#24293018 - 05/03/17 05:32 PM (6 years, 11 months ago) |
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Quote:
bodhisatta said:
Nope 91 is more flammable burns hotter. And i would imagine has lower surface tension to get in more places
butane burns at 3578°F. 91% burns at 750°F.
70% is proven the more effective sterilizer. and the cracks and crevices get red hot from your butane torch.
so I guess im just not seeing the reasoning here. are you not using butane? if not, why not?
Edited by Munchauzen (05/03/17 05:55 PM)
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bodhisatta
Smurf real estate agent
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
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Re: BOD's DIY Inoculation Loop TEK [Re: Munchauzen] 1
#24293145 - 05/03/17 06:40 PM (6 years, 11 months ago) |
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Propane torch anyway. 91% is just always what I've seen used for flaming glass rods. You're not using the alcohol to sanitize
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d0urd3n
Just call me "D"
Registered: 09/15/10
Posts: 5,237
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Re: BOD's DIY Inoculation Loop TEK [Re: Munchauzen]
#24293149 - 05/03/17 06:41 PM (6 years, 11 months ago) |
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Really simple, but great. I have an exacto sitting around since I switched to an actual scalpel. Will be trying this even though regular wire worked just fine. This looks a bit more pro/easier to grab with gloves on.
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Kenetic
Nam Sayin
Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land
Last seen: 5 years, 5 months
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Re: BOD's DIY Inoculation Loop TEK [Re: d0urd3n]
#24293163 - 05/03/17 06:49 PM (6 years, 11 months ago) |
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Damn this is an excellent tek! Too bad i already have one lol. Thats creative though and looks nice.
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Todo Cambia
  
  
DMT said: Everyone know's me, they just don't know it yet
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markymark777
Stranger
Registered: 04/19/17
Posts: 21
Loc: Dayton Oh.
Last seen: 4 years, 9 months
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Re: BOD's DIY Inoculation Loop TEK [Re: bodhisatta]
#24461606 - 07/06/17 10:09 AM (6 years, 9 months ago) |
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I am very inexperienced here, and maybe this is a stupid question, but what is the reason you wouldnt just use the syringe to squirt a drop on the petri dish. Is it just to get the spores to more areas on the dish, or am i missing the point entirely?
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bodhisatta
Smurf real estate agent
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
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Re: BOD's DIY Inoculation Loop TEK [Re: markymark777]
#24461613 - 07/06/17 10:13 AM (6 years, 9 months ago) |
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That is the reason.
You don't want spores all sloppy over a dish
And the more spore solution you use the higher your chance of contamination.
And too much spore solution makes all the growth happen irregularly all over the dish so it's harder to grab glean mycelium.
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mushpunx
Fungus Punk
Registered: 04/20/14
Posts: 13,394
Last seen: 2 months, 7 days
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Re: BOD's DIY Inoculation Loop TEK [Re: bodhisatta]
#24461633 - 07/06/17 10:20 AM (6 years, 9 months ago) |
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Nice write up!
This is exactly the same as the loops they sell on lots of cultivation sites (I think these come from China).
Its just a handle and it comes with a bunch of nichrome wires
You can save yourself some $ doing it yourself
--------------------
 
Amateur Mycologists United
AMU Q&A
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3B3B4E
Turd Stool Hunter
Registered: 08/28/16
Posts: 387
Loc: Somewhere I'm not suppose...
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Re: BOD's DIY Inoculation Loop TEK [Re: mushpunx]
#24461981 - 07/06/17 12:59 PM (6 years, 9 months ago) |
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Cool tek Bod
This is the exact same thing I did, it works perfectly
I didn't use braided wire though, it's there a reason for choosing the braided?
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If the doors of perception were cleansed everything would appear to man as it is, infinite.
- William Blake
  
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bodhisatta
Smurf real estate agent
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
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Re: BOD's DIY Inoculation Loop TEK [Re: 3B3B4E]
#24461988 - 07/06/17 01:01 PM (6 years, 9 months ago) |
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use what you have
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