|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
bodhisatta
Smurf real estate agent
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
|
BOD's DIY Inoculation Loop TEK 13
#24292526 - 05/03/17 01:47 PM (6 years, 10 months ago) |
|
|
This tek is part of bod's cultivation is easy as fuck link listBod's DIY Inoculation Loop TEK Using X-Acto style handles and nichrome wire or easily found as vape coil wireYou Will NeedSome wire, experiment with different lengths 3.5-4 inches works well for me. some sort of thin rod to make the loop with, I used a thermometer and a handle, I used the x-acto for the clamping mechanism. This is how we doTake the x-acto knife apart fold a wire over to make a U shape and put the pointy ends into the blade receiver of the x-acto handle now you have one of these put your thin rod thing through, then push the wire so that it forms a loop around the utensil before you start to twist if you don't do that ugly mess if you spend the time to smooth it out I also buy sterile disposable ones with much smaller loops 1 microliter(uL) they will both work for streaking spores out. With these ones you can bend your loop's eye to make it smaller to hold onto a drop of solution. for example take this then crimp it into this Flame away
|
hamloaf
Pork Block
Registered: 12/23/09
Posts: 21,030
Loc: ation is turned off.
|
Re: BOD's DIY Inoculation Loop TEK [Re: bodhisatta]
#24292533 - 05/03/17 01:49 PM (6 years, 10 months ago) |
|
|
Nice one.
|
Organic_Magic
Medicine Man
Registered: 10/26/14
Posts: 1,712
Loc: Everywhere
Last seen: 2 years, 8 months
|
Re: BOD's DIY Inoculation Loop TEK [Re: bodhisatta]
#24292541 - 05/03/17 01:51 PM (6 years, 10 months ago) |
|
|
This is EXACTLY how I do it. People always forget the versatility of an x-acto handle.
Good stuff bod.
-------------------- Its all fun and games until mushrooms make you gay click and learn. Bod's simplified cultivation methods Got side pins?
|
mushboy
modboy
Registered: 04/24/05
Posts: 32,654
Loc: where?
|
Re: BOD's DIY Inoculation Loop TEK [Re: hamloaf]
#24292545 - 05/03/17 01:53 PM (6 years, 10 months ago) |
|
|
tight
|
mynakedrat
The phantom hourglass
Registered: 02/16/17
Posts: 4,629
Loc: Inner Astral levels
|
Re: BOD's DIY Inoculation Loop TEK [Re: mushboy] 1
#24292551 - 05/03/17 01:55 PM (6 years, 10 months ago) |
|
|
I do this too! I use a bread tie, remove the paper /plastic edges. Awesome once again sir!
|
SuperFly
Still in the Space Race
Registered: 05/19/13
Posts: 1,032
Loc: Dark side off the moon
|
Re: BOD's DIY Inoculation Loop TEK [Re: mynakedrat]
#24292579 - 05/03/17 02:05 PM (6 years, 10 months ago) |
|
|
Quote:
mynakedrat said: I do this too! I use a bread tie, remove the paper /plastic edges. Awesome once again sir!
I do the same thing lol. I was thinking this looks so much nicer than my garbage twisty-tie.
Bod is swiping two prints at one time... Now that's gangster
Edited by SuperFly (05/03/17 02:18 PM)
|
mynakedrat
The phantom hourglass
Registered: 02/16/17
Posts: 4,629
Loc: Inner Astral levels
|
Re: BOD's DIY Inoculation Loop TEK [Re: SuperFly]
#24292583 - 05/03/17 02:07 PM (6 years, 10 months ago) |
|
|
Can contams get in the twists in the loop though? Do you always flame the entire thing?
|
hamloaf
Pork Block
Registered: 12/23/09
Posts: 21,030
Loc: ation is turned off.
|
Re: BOD's DIY Inoculation Loop TEK [Re: mynakedrat]
#24292589 - 05/03/17 02:09 PM (6 years, 10 months ago) |
|
|
I always flame the entire wire.
|
bodhisatta
Smurf real estate agent
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
|
Re: BOD's DIY Inoculation Loop TEK [Re: mynakedrat]
#24292595 - 05/03/17 02:14 PM (6 years, 10 months ago) |
|
|
Quote:
mynakedrat said: Can contams get in the twists in the loop though? Do you always flame the entire thing?
in my agar tek I talk about how I keep a jar of 91% iso like a baby food jar. I dip my tools in there first and then flame them red hot. this way flame gets into all the crevices on tools. This is how a glass spreading rod would be sterilized since you can't hold glass in a flame till it's red hot and then touch a petri dish, it won't cool down fast enough. And it will eventually shatter too.
|
Munchauzen
Registered: 06/22/11
Posts: 14,343
|
Re: BOD's DIY Inoculation Loop TEK [Re: bodhisatta]
#24292742 - 05/03/17 03:25 PM (6 years, 10 months ago) |
|
|
Im sure you meant to say 70%, not 91%.
|
bodhisatta
Smurf real estate agent
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
|
Re: BOD's DIY Inoculation Loop TEK [Re: Munchauzen]
#24292746 - 05/03/17 03:26 PM (6 years, 10 months ago) |
|
|
Nope 91 is more flammable burns hotter. And i would imagine has lower surface tension to get in more places
|
Munchauzen
Registered: 06/22/11
Posts: 14,343
|
Re: BOD's DIY Inoculation Loop TEK [Re: bodhisatta] 2
#24293018 - 05/03/17 05:32 PM (6 years, 10 months ago) |
|
|
Quote:
bodhisatta said: Nope 91 is more flammable burns hotter. And i would imagine has lower surface tension to get in more places
butane burns at 3578°F. 91% burns at 750°F.
70% is proven the more effective sterilizer. and the cracks and crevices get red hot from your butane torch.
so I guess im just not seeing the reasoning here. are you not using butane? if not, why not?
Edited by Munchauzen (05/03/17 05:55 PM)
|
bodhisatta
Smurf real estate agent
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
|
Re: BOD's DIY Inoculation Loop TEK [Re: Munchauzen] 1
#24293145 - 05/03/17 06:40 PM (6 years, 10 months ago) |
|
|
Propane torch anyway. 91% is just always what I've seen used for flaming glass rods. You're not using the alcohol to sanitize
|
d0urd3n
Just call me "D"
Registered: 09/15/10
Posts: 5,237
|
Re: BOD's DIY Inoculation Loop TEK [Re: Munchauzen]
#24293149 - 05/03/17 06:41 PM (6 years, 10 months ago) |
|
|
Really simple, but great. I have an exacto sitting around since I switched to an actual scalpel. Will be trying this even though regular wire worked just fine. This looks a bit more pro/easier to grab with gloves on.
|
Kenetic
Nam Sayin
Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land
Last seen: 5 years, 5 months
|
Re: BOD's DIY Inoculation Loop TEK [Re: d0urd3n]
#24293163 - 05/03/17 06:49 PM (6 years, 10 months ago) |
|
|
Damn this is an excellent tek! Too bad i already have one lol. Thats creative though and looks nice.
-------------------- Todo Cambia DMT said: Everyone know's me, they just don't know it yet
|
markymark777
Stranger
Registered: 04/19/17
Posts: 21
Loc: Dayton Oh.
Last seen: 4 years, 9 months
|
Re: BOD's DIY Inoculation Loop TEK [Re: bodhisatta]
#24461606 - 07/06/17 10:09 AM (6 years, 8 months ago) |
|
|
I am very inexperienced here, and maybe this is a stupid question, but what is the reason you wouldnt just use the syringe to squirt a drop on the petri dish. Is it just to get the spores to more areas on the dish, or am i missing the point entirely?
|
bodhisatta
Smurf real estate agent
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
|
Re: BOD's DIY Inoculation Loop TEK [Re: markymark777]
#24461613 - 07/06/17 10:13 AM (6 years, 8 months ago) |
|
|
That is the reason. You don't want spores all sloppy over a dish And the more spore solution you use the higher your chance of contamination. And too much spore solution makes all the growth happen irregularly all over the dish so it's harder to grab glean mycelium.
|
mushpunx
Fungus Punk
Registered: 04/20/14
Posts: 13,394
Last seen: 1 month, 20 days
|
Re: BOD's DIY Inoculation Loop TEK [Re: bodhisatta]
#24461633 - 07/06/17 10:20 AM (6 years, 8 months ago) |
|
|
Nice write up!
This is exactly the same as the loops they sell on lots of cultivation sites (I think these come from China). Its just a handle and it comes with a bunch of nichrome wires
You can save yourself some $ doing it yourself
-------------------- Amateur Mycologists United AMU Q&A
|
3B3B4E
Turd Stool Hunter
Registered: 08/28/16
Posts: 387
Loc: Somewhere I'm not suppose...
|
Re: BOD's DIY Inoculation Loop TEK [Re: mushpunx]
#24461981 - 07/06/17 12:59 PM (6 years, 8 months ago) |
|
|
Cool tek Bod This is the exact same thing I did, it works perfectly I didn't use braided wire though, it's there a reason for choosing the braided?
-------------------- If the doors of perception were cleansed everything would appear to man as it is, infinite. - William Blake
|
bodhisatta
Smurf real estate agent
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
|
Re: BOD's DIY Inoculation Loop TEK [Re: 3B3B4E]
#24461988 - 07/06/17 01:01 PM (6 years, 8 months ago) |
|
|
use what you have
|
mushpunx
Fungus Punk
Registered: 04/20/14
Posts: 13,394
Last seen: 1 month, 20 days
|
Re: BOD's DIY Inoculation Loop TEK [Re: bodhisatta]
#24462433 - 07/06/17 03:47 PM (6 years, 8 months ago) |
|
|
Do you ever have a problem with the wire melting? I use a propane torch to sterilize my tools and if I let it glow too long it melts. If I'm just doing inoculations I sometimes use a lamp instead, but if I just heat it until it glows and pull it away the torch is fine.
I wonder if my wire is just crappy?
-------------------- Amateur Mycologists United AMU Q&A
|
bodhisatta
Smurf real estate agent
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
|
Re: BOD's DIY Inoculation Loop TEK [Re: mushpunx]
#24462444 - 07/06/17 03:55 PM (6 years, 8 months ago) |
|
|
No after a while it gets carbonized though
|
VitalRiotBoy
Noobie
Registered: 06/04/17
Posts: 86
Last seen: 4 years, 8 days
|
Re: BOD's DIY Inoculation Loop TEK [Re: bodhisatta]
#24498191 - 07/21/17 08:44 AM (6 years, 8 months ago) |
|
|
Would electrical terminal rings work as an inoculation loop. Figured if I crimped one onto the end of a piece of metal it'd be pretty close to purchasing one and cheap and easy to replace as they carbonized.
|
bodhisatta
Smurf real estate agent
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
|
Re: BOD's DIY Inoculation Loop TEK [Re: VitalRiotBoy]
#24498197 - 07/21/17 08:46 AM (6 years, 8 months ago) |
|
|
Try it
|
VitalRiotBoy
Noobie
Registered: 06/04/17
Posts: 86
Last seen: 4 years, 8 days
|
Re: BOD's DIY Inoculation Loop TEK [Re: bodhisatta]
#24498702 - 07/21/17 01:00 PM (6 years, 8 months ago) |
|
|
Here's what they look like hopefully they work, worse comes to worse I drill them out and file them down smooth again. Thoughts?
|
bodhisatta
Smurf real estate agent
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
|
Re: BOD's DIY Inoculation Loop TEK [Re: VitalRiotBoy]
#24498904 - 07/21/17 02:37 PM (6 years, 8 months ago) |
|
|
Might be difficult time flame red hot
|
Kenetic
Nam Sayin
Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land
Last seen: 5 years, 5 months
|
Re: BOD's DIY Inoculation Loop TEK [Re: bodhisatta]
#24498925 - 07/21/17 02:44 PM (6 years, 8 months ago) |
|
|
Yeah that's some pretty thick metal. Creative as fuck though
-------------------- Todo Cambia DMT said: Everyone know's me, they just don't know it yet
|
ediddy
Poppin tops!
Registered: 05/08/17
Posts: 116
Loc: Canada
Last seen: 6 years, 1 month
|
Re: BOD's DIY Inoculation Loop TEK [Re: Kenetic]
#24499147 - 07/21/17 04:14 PM (6 years, 8 months ago) |
|
|
Good idea!
|
36fuckin5
Alchemycologist
Registered: 08/11/03
Posts: 12,083
Loc: Diving into Mystical Territori...
|
Re: BOD's DIY Inoculation Loop TEK [Re: ediddy]
#24499779 - 07/21/17 09:40 PM (6 years, 8 months ago) |
|
|
Kanthal A1 wire is cheaper than nichrome 80 usually, and will hold up better over time. SS316L wire holds up even longer, but costs a little more than nichrome 80. I'd specifically use like 18 or 20 awg wire if I were to do this instead of twisting the wire unless you want your loop to hold more liquid, in which case a Clapton wire (it's like a roundwound guitar string) would do better than twisted.
I like this TEK though. I have lots of wire lying around. And I think an X-acto handle too.
To make the twisting easier, you could just use a drill instead of doing it by hand. Still use your rod, but fasten the loose ends in the chuck, hold tension and twist till it breaks.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
|
VitalRiotBoy
Noobie
Registered: 06/04/17
Posts: 86
Last seen: 4 years, 8 days
|
Re: BOD's DIY Inoculation Loop TEK [Re: bodhisatta]
#24501792 - 07/22/17 10:54 PM (6 years, 8 months ago) |
|
|
Update on terminal ring inoculation loop. As you said doesn't heat up easy. Pretty much impossible with an alcohol lamp. Had it in the blue flame for 10 minutes and only got searing. However propane or butane torch does a wonder. Red hot in seconds (cranked a bit higher then needs be for agar work but it worked). crimping the ring on the end of the rod does wonders for expelling the heat from your hand. Felt warm to the touch after several heat cycles but not over bearing . However the heat that was transferred from the rod was beautifully taken care of by the heat shrink wrapping which gave steady stable precision even with nylon gloves on. However I have to down size a bit with it. 6 gauge terminal rings are way to big to work with on Petri dishes. Plan on finding a smaller rod to use 22 gauge rings on to see if it will make a difference in heating with my alcohol lamp, will test layered heat shrink to see if it makes a difference in heat exchange on the smaller rods and rings. If I can fine tune it I'd like to send you one Bod and get the opinion of aTC. However I'd also like to try out your from the original post. Nothing I buy seems to come with twist ties, any recommended places to pick up wire as it seems like a pain in the ass to cut open a moderate size of my 22 awg sheathing at work.
|
wildernessjunkie
Reshitivest
Registered: 06/13/10
Posts: 8,118
Loc: HTTP 404 Not Found
|
Re: BOD's DIY Inoculation Loop TEK [Re: VitalRiotBoy] 1
#24501822 - 07/22/17 11:12 PM (6 years, 8 months ago) |
|
|
I ordered some inoculation loops online awhile back. While I was waiting for them to arrive I saw this post, ran down to the local ACE Hardware and picked up the Xacto and a roll of SS picture hanging wire. I went with about a 6 inch length. It's way more rigid than the commercial loops and I love it. I look upon the ones I bought with disdain now.
|
bodhisatta
Smurf real estate agent
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
|
|
Ace hardware smoked a lot of weed in the basement of ace hardware before. No one trusts some kid with hardware advice. Same dumbasses would be the ones paying someone to install replacement faucet washers for a drip because they "weren't mechanical people" or some other excuse
|
wildernessjunkie
Reshitivest
Registered: 06/13/10
Posts: 8,118
Loc: HTTP 404 Not Found
|
Re: BOD's DIY Inoculation Loop TEK [Re: bodhisatta]
#24501918 - 07/23/17 12:18 AM (6 years, 8 months ago) |
|
|
50% of everything I need for Myco work I can get there off the shelf over there. Theres also this stoner kid that works there he knows where every item in the store is.
|
VitalRiotBoy
Noobie
Registered: 06/04/17
Posts: 86
Last seen: 4 years, 8 days
|
|
It's always nice to find people like that with this hobby . You ask most people if they carry agar or Light DME and they look at you like your from a different planet.
|
mmmnnn
Stranger
Registered: 07/29/17
Posts: 2
Last seen: 6 years, 7 months
|
Re: BOD's DIY Inoculation Loop TEK [Re: bodhisatta]
#24515622 - 07/29/17 08:00 AM (6 years, 7 months ago) |
|
|
I should try it out, never used one.
|
mekannic
just dropped 2 hits
Registered: 09/28/14
Posts: 238
Last seen: 1 year, 5 months
|
Re: BOD's DIY Inoculation Loop TEK [Re: bodhisatta]
#24590655 - 08/30/17 11:53 AM (6 years, 6 months ago) |
|
|
suggestion on the item to use to form the loop (instead of the thermometer).
Lowe's has some "music wire" in the section with all the small weird hard to find stuff, with all the small nuts and bolts and hobby items. It's actually in the hobby items drawers. They are very small diameter rods made of hardened spring steel. An assortment of diameters, either 4 or 6, or some such. Mine are scattered or I would count them. Ebay has them as well in an absolute plethora of quantities and sizes (diameters). They are usually about 6" long.
you can custom size your inoculation loop to just about any size you desire or could dream up.
-------------------- A lesson on every Element of the Periodic Table The Beautiful Math of Coral Ice Loss Project The Extreme Ice Survey Project Free cubensis prints "the leaf doesn't make it's own decision's. Instead, it is the wind that decides where the leaf shall be, and how long the trip shall take"-m
|
Shroomway
It's me!
Registered: 07/05/17
Posts: 438
|
Re: BOD's DIY Inoculation Loop TEK [Re: Kenetic]
#24590986 - 08/30/17 02:47 PM (6 years, 6 months ago) |
|
|
Quote:
Kenetic said: ...Too bad i already have one lol...
Why don't you just make one for throwing away
btw.: A kickass way to make the loop and spiral is with a drilling machine: make a temporary loop on one end of the wire. That is just for the drilling machine to be able to have a grip on it. Then vise it in the drilling machine like you would with a drill bit. If done correctly it becomes dead straight really easy and quick. Just made one myself (not entirely straight hehehe)
|
mekannic
just dropped 2 hits
Registered: 09/28/14
Posts: 238
Last seen: 1 year, 5 months
|
Re: BOD's DIY Inoculation Loop TEK [Re: bodhisatta]
#24733179 - 10/24/17 02:42 AM (6 years, 5 months ago) |
|
|
another little device, step mandrel
just learned that today
I should have known, I have a ring mandrel, duh
-------------------- A lesson on every Element of the Periodic Table The Beautiful Math of Coral Ice Loss Project The Extreme Ice Survey Project Free cubensis prints "the leaf doesn't make it's own decision's. Instead, it is the wind that decides where the leaf shall be, and how long the trip shall take"-m
|
Severance13
Culture Collector
Registered: 10/03/14
Posts: 196
Last seen: 1 month, 5 days
|
Re: BOD's DIY Inoculation Loop TEK [Re: mekannic]
#24764380 - 11/06/17 04:28 PM (6 years, 4 months ago) |
|
|
Comrade, what gauge do you recommend for nichrome and/or kanthal? I'd like a loop sturdy enough to scrape mycelium off a plate like some of the plastic disposables are capable of doing.
Also, have you ever heard of a mycology tool xalled a "fungus hoe?" According to this link, it seems like a tool everyone on the forum could appreciate. Ctrl-F the term to get to it quickly: http://www.disknet.com/indiana_biolab.
I think 14-18g nichrome would be preferred on the hoe. What are your thoughts?
|
nubgrower
Dude
Registered: 08/27/17
Posts: 274
Loc: Moon 13 the Moon.
Last seen: 6 years, 3 months
|
Re: BOD's DIY Inoculation Loop TEK [Re: Severance13]
#24781043 - 11/13/17 06:10 PM (6 years, 4 months ago) |
|
|
cooooool. I'm going to make one. My latest plates using syringe drops are just a train wreck.
-------------------- Great teks here. And here. Fortunately, I’m adhering to a pretty strict, uh, drug regimen to keep my mind, you know, uh, limber
|
nubgrower
Dude
Registered: 08/27/17
Posts: 274
Loc: Moon 13 the Moon.
Last seen: 6 years, 3 months
|
Re: BOD's DIY Inoculation Loop TEK [Re: nubgrower]
#24782259 - 11/14/17 10:53 AM (6 years, 4 months ago) |
|
|
Bod, I assume that you just lightly slide the loop across the surface when you streak? Not putting any real pressure, not leaving a dent or a cut.....
just making sure. cheers
-------------------- Great teks here. And here. Fortunately, I’m adhering to a pretty strict, uh, drug regimen to keep my mind, you know, uh, limber
|
bodhisatta
Smurf real estate agent
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
|
Re: BOD's DIY Inoculation Loop TEK [Re: nubgrower]
#24782304 - 11/14/17 11:20 AM (6 years, 4 months ago) |
|
|
Yea if it scrapes a bit oh well tho
|
FriendOfTheDevil70
Stranger
Registered: 12/11/17
Posts: 30
Last seen: 3 years, 11 months
|
Re: BOD's DIY Inoculation Loop TEK [Re: bodhisatta]
#24957353 - 01/31/18 03:34 PM (6 years, 1 month ago) |
|
|
I've seen your video using the loop with the print, but are you using a syringe for the initial drop directly onto plate then Z smear with loop? Or where are you getting solution from?
Thanks so much.
EDIT: I saw the answer in your agar tek. Sorry for inconvenience.
-------------------- Set out runnin' but I take my time A friend of the Devil is a friend of mine If I get home before daylight, I just might get some sleep tonight
Edited by FriendOfTheDevil70 (01/31/18 03:36 PM)
|
bodhisatta
Smurf real estate agent
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
|
|
Squirting onto the loop. Or squirt spore solution into a sterilized cup like a shot glass then dip in that
|
dreamwever
shroomster
Registered: 07/06/18
Posts: 93
Loc: Australia
Last seen: 1 year, 8 months
|
Re: BOD's DIY Inoculation Loop TEK [Re: bodhisatta]
#25348409 - 07/26/18 03:49 PM (5 years, 7 months ago) |
|
|
Thanks Bod for the DIY inoculation loop. It seems a better way to get the spores up from the foil. Any spores left will use the 1mm shringe with droplets of distilled water and experiment with remaning spores
Heat the loop and cool in distilled water? is that okay to use
Also, have 20awg audio tinned wire? could this be used? it relatively sturdy, otherwise i can get approxiate wire here in Australia
Few cheep loops on ebay, but shipping ups the price
|
ManifoldPrime
Per Ardua Ad Astra
Registered: 03/16/17
Posts: 1,313
Loc: South Africa
Last seen: 1 year, 3 months
|
Re: BOD's DIY Inoculation Loop TEK [Re: dreamwever]
#25349673 - 07/27/18 03:33 AM (5 years, 7 months ago) |
|
|
Bout time I upgraded my loop. Currently using 2 paper clips bent into a loop and wound on a pencil, taped, and PC'd. Hella ghetto but it works
-------------------- My Magnum Opus:Thai Variety Comparison Please correct me if I say something wrong! | Current Trade Print Availability:Some PESA and RW available for South African posters. Snail Mail only. |
|
bodhisatta
Smurf real estate agent
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
|
Re: BOD's DIY Inoculation Loop TEK [Re: ManifoldPrime] 1
#25349725 - 07/27/18 05:00 AM (5 years, 7 months ago) |
|
|
Yea that's a bit ugly haha
|
dreamwever
shroomster
Registered: 07/06/18
Posts: 93
Loc: Australia
Last seen: 1 year, 8 months
|
Re: BOD's DIY Inoculation Loop TEK [Re: bodhisatta] 1
#25365424 - 08/03/18 10:11 PM (5 years, 7 months ago) |
|
|
my 2 DIY Innoc loops 28awg nichrome wire
|
Rowboat
Nincompoop
Registered: 08/24/18
Posts: 47
Last seen: 2 years, 2 months
|
Re: BOD's DIY Inoculation Loop TEK [Re: bodhisatta]
#25478954 - 09/21/18 07:18 PM (5 years, 6 months ago) |
|
|
Quote:
Bod's DIY Inoculation Loop TEK Using X-Acto style handles and Nicrhome wire or easily found as vape coil wire
This being apart of such a great source of information, I figured I'd mention Nichrome is misspelled here in the title if it matters.
--------------------
|
bodhisatta
Smurf real estate agent
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
|
Re: BOD's DIY Inoculation Loop TEK [Re: Rowboat]
#25479151 - 09/21/18 08:22 PM (5 years, 6 months ago) |
|
|
Good catch haha
|
ttching8475
Spawn
Registered: 03/01/18
Posts: 739
|
Re: BOD's DIY Inoculation Loop TEK [Re: bodhisatta]
#25479304 - 09/21/18 09:33 PM (5 years, 6 months ago) |
|
|
Well timed bump of this thread.
-------------------- LAGM 2.022
|
Severance13
Culture Collector
Registered: 10/03/14
Posts: 196
Last seen: 1 month, 5 days
|
Re: BOD's DIY Inoculation Loop TEK [Re: ttching8475]
#26009310 - 05/23/19 12:42 PM (4 years, 10 months ago) |
|
|
Alright everyone. I know Bod suggests using whatchu got for simplicity, but I did some research, and it turns out 25 or 26 g kanthal or nichrome 80 would be optimal. The thinness guarantees better cooldown times. I noticed my current one made with 20g kanthal sizzles my agar during cooldown for nearly 30 sec. As we all know, exposure time matters in this hobby.
I also posted a link earlier about another tool, a Fungus Hoe, that seems to be perfect for ripping and transferring mycelium to new medium. Here's an updated link. What do y'all think?
|
wildernessjunkie
Reshitivest
Registered: 06/13/10
Posts: 8,118
Loc: HTTP 404 Not Found
|
Re: BOD's DIY Inoculation Loop TEK [Re: Severance13]
#26010376 - 05/24/19 12:50 AM (4 years, 10 months ago) |
|
|
I used stainless steel picture hanging wire from the hardware store for my first loop. Works great, and I still use it.
The pics in your that link you posted are broken BTW.
|
Sir Pentinite
Stranger all the time.
Registered: 05/15/19
Posts: 525
Loc: ation Location Location
|
|
Since I've been dragging my feet on making an introduction thread, maybe my first post can be a contribution.
One way to scavenge Nichrome wire is from wirewound electrical resistors. They are typically found as the rectangular ceramic type meant to handle a lot of current and heat. I broke open a 10 ohm/10 watt power resistor and it had about 14" of 32awg Nichrome. Lower ohms with higher wattage ratings would probably have thicker wire.
I wouldn't suggest buying resistors just for the wire, but if you already have some...
-------------------- "I thought to myself 'Boy, I'm sure glad there's nobody here to see this because this is exactly the sort of thing that gets people riled-up and they assume you're dying and that something has to be done. Where if you're alone, you know, you either come through it or you die, but in any case you avoid the fuss.'" - Terrence McKenna
|
Danito
Registered: 10/19/18
Posts: 41
Last seen: 4 years, 2 months
|
Re: BOD's DIY Inoculation Loop TEK [Re: bodhisatta]
#26133990 - 08/13/19 03:34 PM (4 years, 7 months ago) |
|
|
Great stuff Bod thank you. I spent an hour earlier inoculating with one of those little "1 microliter" loops you mentioned, and afterwards started to worry that it might have been too small for the job. I bought them in complete noob ignorance from Ebay.
Three quick questions please:
1. Being plastic and not able to be flamed, do I need to use a different loop with each petri?
2. Can I PC the used loops to re-sterilise them?
3. Is there any advantage to using the pre-sterilised loops over following your DIY loop tek?
-------------------- "Our culture is not our friend" Yuval Noah Harari Change your self, don't try to change the world
|
bodhisatta
Smurf real estate agent
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
|
Re: BOD's DIY Inoculation Loop TEK [Re: Danito]
#26134047 - 08/13/19 04:02 PM (4 years, 7 months ago) |
|
|
1. Yes 2. Probably not 3. If you like the disposables
|
vinnie boombotz
Reggaejunkiejew
Registered: 04/29/19
Posts: 493
Last seen: 8 days, 4 hours
|
Re: BOD's DIY Inoculation Loop TEK [Re: bodhisatta] 1
#26206481 - 09/24/19 12:38 PM (4 years, 6 months ago) |
|
|
Incredible. This is a must, anyone who works with agar needs to make these
|
mi.ro.ku
Stranger
Registered: 04/13/19
Posts: 83
Last seen: 2 years, 7 months
|
|
Good tek
|
DeadlySin
Student of Shroomery
Registered: 09/25/19
Posts: 69
Last seen: 3 years, 5 months
|
Re: BOD's DIY Inoculation Loop TEK [Re: mi.ro.ku]
#26247135 - 10/12/19 11:02 AM (4 years, 5 months ago) |
|
|
Great Tek. Here is my attempt.
|
bodhisatta
Smurf real estate agent
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
|
Re: BOD's DIY Inoculation Loop TEK [Re: DeadlySin]
#26247201 - 10/12/19 11:40 AM (4 years, 5 months ago) |
|
|
Looks like more of a success than an attempt
|
DeadlySin
Student of Shroomery
Registered: 09/25/19
Posts: 69
Last seen: 3 years, 5 months
|
Re: BOD's DIY Inoculation Loop TEK [Re: bodhisatta]
#26249149 - 10/13/19 07:16 AM (4 years, 5 months ago) |
|
|
Thanks mate. Helps having a well written guide Inoculated some agar with it yesterday. Didn't bend or anything. Really sturdy and easy to use tool. First ever round of inoculation, just waiting to see how clean and thorough I was.
Also wanted to thank you for all your teks / guides. Been plenty of reading, but a lot of help too.
|
Raleigh69
Diligility
Registered: 05/29/18
Posts: 59
Last seen: 7 months, 6 days
|
Re: BOD's DIY Inoculation Loop TEK [Re: bodhisatta]
#26430647 - 01/13/20 03:24 AM (4 years, 2 months ago) |
|
|
Found these
At Walmart for $1.82 in the makeup section
-------------------- Take the path, of most resistance
|
shevanel
Gone til November
Registered: 01/27/08
Posts: 1,517
Last seen: 2 years, 1 month
|
Re: BOD's DIY Inoculation Loop TEK [Re: Raleigh69]
#26498172 - 02/22/20 09:04 AM (4 years, 1 month ago) |
|
|
I just used 1ul tiny displosable plastic loops.
My concern with it being so small does it still transfer enough spores?
Something about not squirting a drop feels like i didnt do anything to innoc my plates lol
|
Doctor Mario
🍄🍄🍄🍄🍄
Registered: 08/07/20
Posts: 1,894
Loc: 🇺🇸
|
Re: BOD's DIY Inoculation Loop TEK [Re: bodhisatta]
#26897600 - 08/24/20 06:03 PM (3 years, 6 months ago) |
|
|
Thank you, sir.
Edited by Doctor Mario (08/28/20 09:23 PM)
|
Grogu
Registered: 07/02/22
Posts: 1,712
Last seen: 10 months, 9 days
|
Re: BOD's DIY Inoculation Loop TEK [Re: Doctor Mario]
#27870997 - 07/20/22 10:08 PM (1 year, 8 months ago) |
|
|
When one flames the wire or scaple, syringe gets black suut on it, does that have to be cleaned with ISO and it seems hardcore to flame red hot like these contams are so resilant and stuff only red hot will kill them!?
And like with a syringe can the heat kill the spores so wait till cooler then clean with ISO?
Thanks...
|
PsillySeeEms
Stranger
Registered: 11/12/22
Posts: 608
Loc: PNW
|
Re: BOD's DIY Inoculation Loop TEK [Re: bodhisatta]
#28116657 - 12/29/22 06:32 PM (1 year, 2 months ago) |
|
|
Has anyone tried hooking a coiled up version of a loop to a vape mod? Just like you would make a vape coil, but leave the leads really long (and not twisted up), so only the coiled loop section at the end heats up. One end could be the main loop, so you have 2 ends to work with each time. Or would it wick up too much solution?
Anyway, you could just push the button to fire it up and burn it off between use, instead of having to flame it each time, they way i'm thinking about it...
I was looking through my vape supplies to get some kanthal wire for this tek, to make my own little tool, and couldn't help but wonder if some one used a vape mod as the handle, so they could sterilize with battery power...
Edited by PsillySeeEms (12/29/22 06:36 PM)
|
NuclearTidalWave
Dyler Turden
Registered: 09/07/16
Posts: 374
Loc: Simulation #7
Last seen: 1 month, 1 day
|
Re: BOD's DIY Inoculation Loop TEK [Re: PsillySeeEms]
#28116666 - 12/29/22 06:35 PM (1 year, 2 months ago) |
|
|
Quote:
PsillySeeEms said: Has anyone tried hooking a coiled up version of a loop to a vape mod? Just like you would make a coil, but leave the leads really long, so only the coiled loop section at the end heats up.
Then, you could fire the trigger and burn it off between use, instead of having to flame it.
Anyway, I was looking through my vape supplies to get some kanthal wire, to make my own little tool, and couldn't help but wonder if some one used a vape mod as the handle, so they could sterilize with battery power...
I have no idea what to search for to find the post but yes this has been done.
Edit: Check this out
-------------------- Signature text goes here P̫̻̳̮͎̥̬͋͊̂͐Ä̡͓̜̩̤̠̮́́̔̽̇Ṛ̹̱ͅA̠͉͒F̹̦̣͇̘̻̍̎ͬ͋͒̅͌͘I̖̻ͨͧ̇̋ͯ͡L̩̩͋́M͕̳͎̱̎ ̤̜͇̰̉̿ͫ̑͑͡ͅC̶̲̝̉̔U͚̙͕̬̘̗̐ͮ̀ͣ̎L̏́̂͋Ṯ̥̪̠ͩ͛̃͊ .
Edited by NuclearTidalWave (12/29/22 06:38 PM)
|
PsillySeeEms
Stranger
Registered: 11/12/22
Posts: 608
Loc: PNW
|
|
Nice! I have lots of old vape mods laying around, and a few spools of different kinds of wire. I'll see if I can come up with a nice looking tool, and be able to make a bunch of coils up, so i'm always ready to go.
I'll search for the threads you speak of tonight to see how good it works, before I get too involved. In the meantime, I'll be twisting a few up
|
PsillySeeEms
Stranger
Registered: 11/12/22
Posts: 608
Loc: PNW
|
Re: BOD's DIY Inoculation Loop TEK [Re: PsillySeeEms]
#28116679 - 12/29/22 06:46 PM (1 year, 2 months ago) |
|
|
|
stubb
Dahg Rastubfari
Registered: 03/23/19
Posts: 1,339
Loc: Memory
|
Re: BOD's DIY Inoculation Loop TEK [Re: Grogu]
#28116966 - 12/29/22 11:40 PM (1 year, 2 months ago) |
|
|
Quote:
Grogu said:When one flames the wire or scaple, syringe gets black suut on it, does that have to be cleaned with ISO and it seems hardcore to flame red hot like these contams are so resilant and stuff only red hot will kill them!
Sorry your question got overlooked, but no, the black soot doesn't hurt anything. Yes, many contams are that resilient. It just seems hardcore to us because our meatsacks are pathetically fragile.
Quote:
And like with a syringe can the heat kill the spores so wait till cooler then clean with ISO?
No, never wipe sterile tools!
- Dip your inoculation loop, scalpel, or whathaveyou into a sterile agar dish to cool it.
- Just plunge the syringe, the flowing liquid cools the needle almost instantly.
-------------------- 🆃🄴🅰🄼 🅲🄻🅸🄽🅶🅆🆁🄰🅿 You wake up. The room is spinning very gently round your head. Or at least it would be if you could see it which you can't. It is pitch black. > TURN ON LIGHT
|
stakesalad
Stranger
Registered: 08/09/23
Posts: 34
Last seen: 2 months, 24 days
|
Re: BOD's DIY Inoculation Loop TEK [Re: stubb]
#28464346 - 09/10/23 12:55 PM (6 months, 13 days ago) |
|
|
Does Galvanized wire hold up to hitting it with a torch?
I used some 19 gauge and it came out looking ok... I just don't know if that protective layer of zinc is going to hurt me or my attempts at mycology somehow.
Edited by stakesalad (09/10/23 12:56 PM)
|
NotFromGeorgia
it's 'Mr. Stranger' to you
Registered: 07/18/23
Posts: 332
|
Re: BOD's DIY Inoculation Loop TEK [Re: stakesalad] 1
#28464393 - 09/10/23 01:42 PM (6 months, 13 days ago) |
|
|
don't used galvanized the zinc layer will peel off after repeated heat cycles. probably don't have to worry about inhaling vapors at torch temperatures, but that's also bad if it does happen
-------------------- The most important step a man can take. It's not the first one, is it? It's the next one. Always the next step, Dalinar.
|
|