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Offlinemellyjane
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Bacterial Monotub? Pics
    #24268074 - 04/24/17 08:52 AM (7 years, 8 months ago)

Realizing this might be bacterial. Agar to WBS to coir/verm. Colonized about 16 days (too long in hindsight).

Smelled really earthy and mushroomy. Not bad at all. Cased yesterday but now concerned it was bacterial. Any help very appreciated




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Offlinemellyjane
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Re: Bacterial Monotub? Pics [Re: mellyjane]
    #24268077 - 04/24/17 08:54 AM (7 years, 8 months ago)

By the way. Was all white until yesterday I cut the bag down and a few yellow droplets fell on it.

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InvisibleMad Season
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Re: Bacterial Monotub? Pics [Re: mellyjane]
    #24268317 - 04/24/17 11:36 AM (7 years, 8 months ago)

The myc does look thick and cottage cheese like. That does suggest bacteria. Looks like some areas are having trouble colonising, is it in fruiting conditions? If not you should induce fruiting.


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Offlinemellyjane
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Re: Bacterial Monotub? Pics [Re: Mad Season]
    #24268334 - 04/24/17 11:49 AM (7 years, 8 months ago)

Thank you! I've been playing with agar and grains. This was first of two mono tubs. So I've learned a ton

One thing I did wrong- I was thinking I should see wall to wall myc on top to indicate full colonization. I also had too much moisture.

Anyways- I opened holes and put polyfill in them, cases with sterilized jiffy seed started and started 12 hr lighting.

Any chance that the casing would impact the bacteria? Is it really a waiting game now to see if it fruits and if so if the fruits look bacterial?

Am I better off burying it out back?

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Offlinemellyjane
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Re: Bacterial Monotub? Pics [Re: mellyjane]
    #24268340 - 04/24/17 11:54 AM (7 years, 8 months ago)

I see what you mean now about areas that didn't colonise. Crap

I can stand the hit if I need to dump it. Plenty of years to get it right left

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InvisibleMad Season
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Re: Bacterial Monotub? Pics [Re: mellyjane]
    #24268346 - 04/24/17 11:56 AM (7 years, 8 months ago)

It's more a waiting game between if the myc can hold its own against molds or not. Bacteria is always the first step to mold. In fact a clean substrate free of bacteria doesn't mold at all.

Bacteria isn't anything to worry about besides that. It won't affect your fruit bodies, and they'll all be edible. Mushrooms have evolved with all sorts of bacteria, and literally every patch in the wild will have bacteria in it, but they never transfer to the fruits. It's not like every person picking mushrooms goes to the hospital :wink:.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.

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Offlinemellyjane
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Re: Bacterial Monotub? Pics [Re: Mad Season]
    #24268376 - 04/24/17 12:12 PM (7 years, 8 months ago)

I can't express my gratitude for your reply and your insight.

Bacteria is my consistent contam issue. My work place is just not clean enough. I'm going to work on creating another one.

To make the comedy of errors even better yesterday- I got the stomach flu overnight. I'm sure it was present in my body but undetected by me while I was opening the holes and trimming the black trash bag yesterday. So surely I breathed this illness over it.

So many lessons.

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Offlinemellyjane
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Re: Bacterial Monotub? Pics [Re: Mad Season]
    #24270041 - 04/25/17 05:59 AM (7 years, 8 months ago)

I can't express my gratitude for your reply and your insight.

Bacteria is my consistent contam issue. My work place is just not clean enough. I'm going to work on creating another one.

To make the comedy of errors even better yesterday- I got the stomach flu overnight. I'm sure it was present in my body but undetected by me while I was opening the holes and trimming the black trash bag yesterday. So surely I breathed this illness over it.

So many lessons.

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Offlinejda
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Re: Bacterial Monotub? Pics [Re: mellyjane]
    #24278120 - 04/28/17 08:04 AM (7 years, 8 months ago)

My question is what's going on here?? Comments appreciated!
I have a similar problem using WBS in a monotub with commercially made dung substrate. This was birthed 14 day ago.


In contrast, I tried two bags and they have fruited (also 14 days since birthing) (they had a LOT of condensate)I removed substrate from bag to fruit,

And here is a small monotub that was birthed 8 days ago, also,

These are my first grows in 7 years,,, never had a problem before,,, "Past performance is no indicator of future returns" LOL

Edited by jda (04/28/17 08:40 AM)

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