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mynakedrat
The phantom hourglass



Registered: 02/16/17
Posts: 4,629
Loc: Inner Astral levels
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Re: Does Coffee Have Anti-fungal Properties? [Re: Kevlar123]
#24278227 - 04/28/17 08:56 AM (7 years, 8 months ago) |
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Pigeon holing, put into a classification. So you aren't human, and prone to Mistakes? You aren't pressure cooking long enough. You got lucky they all didn't get fucked up.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,915
Loc: Milky way
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Re: Does Coffee Have Anti-fungal Properties? [Re: mynakedrat]
#24278237 - 04/28/17 09:01 AM (7 years, 8 months ago) |
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K, if you want to learn you will.
What you don't want to hear is the answer. when you can handle hearing that you fucked it up and it had nothing inherently to do with coffee we'll be here to help.
Coffee is a contamination magnet. All your jars are bacterial anyway bacteria just got more busy in the coffee jars and the mycelium couldn't do anything. The other jars have bacterial mycelium
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Kevlar123
Stranger
Registered: 03/25/17
Posts: 74
Last seen: 7 years, 5 months
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Re: Does Coffee Have Anti-fungal Properties? [Re: mynakedrat]
#24278245 - 04/28/17 09:04 AM (7 years, 8 months ago) |
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PCing for for 60 minutes is widely reported to be long enough on this forum.
How does that even change the parameters? The only thing different between the jars is the coffee beans. Saying your human and prone to mistakes is just a cunty way to say go fuck yourself, and falsely present some faillicy to my results. Under the circumstances I presented coffee certainly does have a statistically significant effect on myc growth. And it has some peer review published research to back it up. Every human is prone to error for sure that's why we have statistics and peer review.
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Kevlar123
Stranger
Registered: 03/25/17
Posts: 74
Last seen: 7 years, 5 months
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Re: Does Coffee Have Anti-fungal Properties? [Re: Kevlar123]
#24278251 - 04/28/17 09:07 AM (7 years, 8 months ago) |
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K, dude.
RR says it's not a contamination magnet btw. Again you try and turn this into a personal attack but I was just presenting my observations. You know adding to the mushroom cultivation forum. Peer reviewed reseach from multiple sources is just confrontational bias. I'll remember that in future.
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AndyHinton


Registered: 12/05/16
Posts: 434
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Re: Does Coffee Have Anti-fungal Properties? [Re: Kevlar123]
#24278254 - 04/28/17 09:09 AM (7 years, 8 months ago) |
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Dude, no comparison is possible here. The single most important factor that affects all the others, the actual strain itself, is completely uncontrolled.
You're growing a different and impossible-to-assess spore polyculture in each jar of what appears to be improperly sterilized grains. The whole beans don't help either, as those simply aren't used in any stage of mush cult.
If you want to do a meaningful comparison, please see the last section of my GLC primer for a nice experiment you can try. I'm genuinely interested in the results, but only if you control for the strain.
--------------------
Edited by AndyHinton (12/22/17 11:58 AM)
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,915
Loc: Milky way
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Re: Does Coffee Have Anti-fungal Properties? [Re: Kevlar123]
#24278267 - 04/28/17 09:15 AM (7 years, 8 months ago) |
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You contaminated all your jars The coffee ones got even more fucked
You'll realize you fucked it up when you try to spawn those "clean" jars and you get a bunch of mold before mushrooms
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,999
Loc:
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Re: Does Coffee Have Anti-fungal Properties? [Re: AndyHinton]
#24278272 - 04/28/17 09:17 AM (7 years, 8 months ago) |
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You need to sterilize moar Kebap123, let's do shit right and then get all scientific about it, no Ads here.
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hamloaf
Q-dood ®©™√


Registered: 12/23/09
Posts: 24,389
Loc: ation: Based.
Last seen: 14 hours, 35 minutes
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Re: Does Coffee Have Anti-fungal Properties? [Re: bodhisatta]
#24278281 - 04/28/17 09:19 AM (7 years, 8 months ago) |
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Quote:
dhype773 said: I thought you use coffee with oysters to boost nitrogen
Nitrogen addition is one of the benefits of using spent coffee grounds, you are correct.
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Kevlar123
Stranger
Registered: 03/25/17
Posts: 74
Last seen: 7 years, 5 months
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Re: Does Coffee Have Anti-fungal Properties? [Re: bodhisatta]
#24278290 - 04/28/17 09:21 AM (7 years, 8 months ago) |
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Aight bro, Why did only the coffee ones "get more fucked up" then than the none coffee ones when the all had the exact same treatment. Under these circumstances why would you not think that the coffee was also a factor or are you suggesting completely down to chance and coffee has no effect?
we will see, in the name of science i'll try to grow shrooms from all of these jars.
Peace Kevlar
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mynakedrat
The phantom hourglass



Registered: 02/16/17
Posts: 4,629
Loc: Inner Astral levels
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Re: Does Coffee Have Anti-fungal Properties? [Re: Kevlar123]
#24278292 - 04/28/17 09:24 AM (7 years, 8 months ago) |
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Did you put genetic cloned mycelium isolate into each jar? Or a Multi spore syringe
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mynakedrat
The phantom hourglass



Registered: 02/16/17
Posts: 4,629
Loc: Inner Astral levels
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Re: Does Coffee Have Anti-fungal Properties? [Re: Kevlar123]
#24278296 - 04/28/17 09:25 AM (7 years, 8 months ago) |
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Did you put genetic cloned mycelium isolate into each jar? Or a Multi spore syringe
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Myco Mako
He who speaks with fungus



Registered: 03/13/17
Posts: 687
Loc: Mars
Last seen: 7 years, 6 months
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Re: Does Coffee Have Anti-fungal Properties? [Re: mynakedrat]
#24278314 - 04/28/17 09:37 AM (7 years, 8 months ago) |
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60 minutes PC not adequate for sterilization?
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Kevlar123
Stranger
Registered: 03/25/17
Posts: 74
Last seen: 7 years, 5 months
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Re: Does Coffee Have Anti-fungal Properties? [Re: mynakedrat]
#24278318 - 04/28/17 09:38 AM (7 years, 8 months ago) |
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Yeah it was a multispore, I'm not saying the generics were identical.
Consider if they were testing a new drug, they wouldn't be able to test it on genetically identically people. Say they had 15 genetically diverse people all with cancer and they gave 5 (Coffee jars) an anti-cancer drug and gave the remaining 10 (non-coffee jars) a placebo and at the end of the study only the 5 (coffee jars) given the drug were free of cancer while the other 10 that were given the placebo still had cancer. You would then do a statistical analysis and probably think that when trying to control for as many factors as possible that the drug probably (not definitely) had a statistically significant effect on destroying the cancer.
Then the scientists looked at other studies which showed the drug had anti-cancer properties when testing in rats, confirmational bias? or just standard scientific procedure?
I just wanna present my observations, honestly is not what this forum is for?
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,915
Loc: Milky way
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Re: Does Coffee Have Anti-fungal Properties? [Re: Kevlar123]
#24278321 - 04/28/17 09:39 AM (7 years, 8 months ago) |
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Quote:
Kevlar123 said: Aight bro, Why did only the coffee ones "get more fucked up" then than the none coffee ones when the all had the exact same treatment. Under these circumstances why would you not think that the coffee was also a factor or are you suggesting completely down to chance and coffee has no effect?
we will see, in the name of science i'll try to grow shrooms from all of these jars.
Peace Kevlar
The coffee is more food for the bacteria in all of your jars. The bacteria is so rampant in the coffee jars that the mycelium didn't recover at all. In the non coffee jars you have some recovery but they look like shit too.
Your "observations" are you not admitting you don't know shit about growing mushrooms and trying to blame it on ingredients
Once you get over thinking you did it right you'll be able to work on doing it right. If you blame coffee you'll keep fucking it up
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Kevlar123
Stranger
Registered: 03/25/17
Posts: 74
Last seen: 7 years, 5 months
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Re: Does Coffee Have Anti-fungal Properties? [Re: bodhisatta]
#24278335 - 04/28/17 09:47 AM (7 years, 8 months ago) |
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Quote:
bodhisatta said:
Quote:
Kevlar123 said: Aight bro, Why did only the coffee ones "get more fucked up" then than the none coffee ones when the all had the exact same treatment. Under these circumstances why would you not think that the coffee was also a factor or are you suggesting completely down to chance and coffee has no effect?
we will see, in the name of science i'll try to grow shrooms from all of these jars.
Peace Kevlar
The coffee is more food for the bacteria in all of your jars. The bacteria is so rampant in the coffee jars that the mycelium didn't recover at all. In the non coffee jars you have some recovery but they look like shit too.
Your "observations" are you not admitting you don't know shit about growing mushrooms and trying to blame it on ingredients
Once you get over thinking you did it right you'll be able to work on doing it right. If you blame coffee you'll keep fucking it up
Well I never said I knew shit about growing mushrooms and thats why I'm here. Again with the personal attacks. But thanks for replying mate.
I guess you should write to all those peer reviewed journals and tell them they are wrong and if they disagree it's confirmation bias and humans make mistakes.
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Myco Mako
He who speaks with fungus



Registered: 03/13/17
Posts: 687
Loc: Mars
Last seen: 7 years, 6 months
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Re: Does Coffee Have Anti-fungal Properties? [Re: Myco Mako]
#24278347 - 04/28/17 09:55 AM (7 years, 8 months ago) |
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Quote:
Myco Mako said: 60 minutes PC not adequate for sterilization?

Disregard... Another cup of coffee sorted it out... 6 vs 9 just didn't compute obviously...
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Kevlar123
Stranger
Registered: 03/25/17
Posts: 74
Last seen: 7 years, 5 months
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Re: Does Coffee Have Anti-fungal Properties? [Re: Myco Mako]
#24278371 - 04/28/17 10:06 AM (7 years, 8 months ago) |
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,915
Loc: Milky way
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Re: Does Coffee Have Anti-fungal Properties? [Re: Kevlar123]
#24278387 - 04/28/17 10:16 AM (7 years, 8 months ago) |
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You're not understanding anything im saying then twisting it to protect your ego.
Quote:
I guess you should write to all those peer reviewed journals and tell them they are wrong and if they disagree it's confirmation bias and humans make mistakes.
I was talking about you and the articles you found having nothing to correlate to what we're doing. I didn't say anything about the articles themselves or their contents.
I can post irrelevant articles too https://www.ncbi.nlm.nih.gov/m/pubmed/16049733/
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Kevlar123
Stranger
Registered: 03/25/17
Posts: 74
Last seen: 7 years, 5 months
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Re: Does Coffee Have Anti-fungal Properties? [Re: bodhisatta]
#24278401 - 04/28/17 10:25 AM (7 years, 8 months ago) |
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Quote:
bodhisatta said: You're not understanding anything im saying then twisting it to protect your ego.
Quote:
I guess you should write to all those peer reviewed journals and tell them they are wrong and if they disagree it's confirmation bias and humans make mistakes.
I was talking about you and the articles you found having nothing to correlate to what we're doing. I didn't say anything about the articles themselves or their contents.
I can post irrelevant articles too https://www.ncbi.nlm.nih.gov/m/pubmed/16049733/
The title of this post was 'Does coffee have anti-fungal properties?'. We grow fungus and we use coffee, how is that not related?. Every fucking sentenced is like loaded spring to cut into my credibility and motivations. I mean why would anyone even think to consider actual science when you just try and discredit the person you arguing with.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,915
Loc: Milky way
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Re: Does Coffee Have Anti-fungal Properties? [Re: Kevlar123]
#24278417 - 04/28/17 10:30 AM (7 years, 8 months ago) |
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Your asking the wrong question dude. You came here thinking your coffee jars were not growing because of the coffee. Its you and your technique.
Even if coffee has some anti fungal properties it's well documented that mold grows rampantly on it and people have grown cubensis from straight coffee as well for fucks sake.
Get over the science bullshit and open your mind to learning where you fucked up
You're talking to me like I don't consider science. Im the one people go to for peer reviewed articles because I have access to most of it and can put them back into layman's terms.
Your statistics class and journal review midterm assignments ain't going to help you grow mushrooms
I do believe you came here to figure out how to grow mushrooms not pick our brain about coffee being anti fungal in a place where people grow fungi on coffee
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