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OfflineKevlar123
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Does Coffee Have Anti-fungal Properties?
    #24277895 - 04/28/17 05:50 AM (7 years, 8 months ago)

2 weeks ago I inoculated a bunch of jars with spores and had some coffee beans I got last Christmas lying around so I thought to experiment I'll add some to a few of the jars.  All other variables are the same and I inoculated them in a random order and all were randomly PC's at 15 psi for 1 hour.  But the jars with coffee added took much longer to show any signs of colonisation and the mycelium is growing much slower.

The jars in the middle are the coffees and every jar has shown at least a small amount of growth.


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Invisiblemynakedrat
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Re: Does Coffee Have Anti-fungal Properties? [Re: Kevlar123]
    #24277967 - 04/28/17 06:57 AM (7 years, 8 months ago)

No. Mold grows on coffee easily

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OfflineKevlar123
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Re: Does Coffee Have Anti-fungal Properties? [Re: mynakedrat]
    #24278002 - 04/28/17 07:14 AM (7 years, 8 months ago)

It's still entirely possible for coffee to have anti-fungal agents and some fungal types to be able to grow on it easily.

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Invisiblemynakedrat
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Re: Does Coffee Have Anti-fungal Properties? [Re: Kevlar123]
    #24278030 - 04/28/17 07:25 AM (7 years, 8 months ago)

I use coffee in all my Subs.
Never an issue.
Maybe maxwell is better than folders or Starbucks?
I think so

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InvisibleLeftfield420
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Re: Does Coffee Have Anti-fungal Properties? [Re: Kevlar123]
    #24278034 - 04/28/17 07:26 AM (7 years, 8 months ago)

No...that's why you have to pasteurize anything you add coffee to

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OfflineKevlar123
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Re: Does Coffee Have Anti-fungal Properties? [Re: Leftfield420]
    #24278044 - 04/28/17 07:29 AM (7 years, 8 months ago)

Hello,

The coffee was PC'd for 60 minutes, same as all of the other jars.  The only difference between them is that some have a few coffee beans added.  This for some reason seems to have slowed growth considerably.  The Myc is still growing on the coffee just much slower.

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Invisiblemynakedrat
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Re: Does Coffee Have Anti-fungal Properties? [Re: Kevlar123]
    #24278046 - 04/28/17 07:30 AM (7 years, 8 months ago)

Yea beans... I don't think they are edible for the myc. How much?

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OfflineKevlar123
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Re: Does Coffee Have Anti-fungal Properties? [Re: Kevlar123]
    #24278060 - 04/28/17 07:37 AM (7 years, 8 months ago)

Anyway I was having a quick look on google scholar.  Seems like coffee might slow down fungal colony formation, so probably better not to use it in spawn jars.

An antifungal peptide from Coffea canephora seeds with sequence homology to glycine-rich proteins exerts membrane permeabilization and nuclear localization in fungi.

Or it might just be the caffeine which would be most removed by the brewing process.

1,3,7-Trimethylxanthine (caffeine); a new natural fish fungicide

In vitro studies on antifungal activity of tea (Camellia sinensis) and coffee (Coffea arabica) against wood-rotting fungi

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OfflineKevlar123
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Re: Does Coffee Have Anti-fungal Properties? [Re: mynakedrat]
    #24278065 - 04/28/17 07:39 AM (7 years, 8 months ago)

Quote:

mynakedrat said:
Yea beans... I don't think they are edible for the myc. How much?




120g of coffee beans spread out over 5 jars.  I think they will eventually colonise, it will just take a lot longer.

Cheers

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OfflineKevlar123
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Re: Does Coffee Have Anti-fungal Properties? [Re: Kevlar123]
    #24278075 - 04/28/17 07:43 AM (7 years, 8 months ago)

Anyway to quote another study,  I'm are sure coffee has some effect on myc growth.  Added to bulk it probally makes very little difference but when the spores are starting out it's gonna make a huge difference.  Once the myc has colonised the coffee I'm sure the coffee nutrients are beneficial overall.

Quote:

For C. coffeicola the reduction of mycelial growth was 64.48%, when extracts of A. sativum, V. polysphaera and S. aromaticum were applied. Chalfoun et al. (2009) observed a reduction of 5.05% on the mycelial growth of C. coffeicola, when 10 µL of methanol extract of C. longa was applied on top of the disk that contained the fungus. By using the extracts of A. sativum, V. polysphaera, C. nardus and S. aromaticum against R. solani was observed a mycelial growth inhibition of 100%. The different results obtained using several species as biofungicides extracts suggests that there are many substances, which can still be exploited for the management of plant pathogens.



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Invisiblemynakedrat
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Re: Does Coffee Have Anti-fungal Properties? [Re: Kevlar123]
    #24278102 - 04/28/17 07:55 AM (7 years, 8 months ago)

Oh in a spawn jar?
No no.
Just grains
I add spent coffee grounds to the substrate when spawning to buckets.
Sorry

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Re: Does Coffee Have Anti-fungal Properties? [Re: mynakedrat]
    #24278117 - 04/28/17 08:02 AM (7 years, 8 months ago)

No comparison is possible in this case because each jar is growing a different culture.

Coffee is a good microbe substrate in my experience; it molds over as fast as bread. It's also acidic and caffeinated, which are two undesirable qualities for mush cult.

I add the last espresso grounds to (sterilized) fruiting substrates. If anything, it gives traditional supplements like wheat bran a little kick.

For whatever reason, oysters seem to really love it.


--------------------

Edited by AndyHinton (12/20/17 06:43 PM)

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Invisiblemynakedrat
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Re: Does Coffee Have Anti-fungal Properties? [Re: AndyHinton]
    #24278129 - 04/28/17 08:07 AM (7 years, 8 months ago)

Good info thanks.
I left  coffee put of My newest tub, was out of coffee lol

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Offlinedhype773
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Re: Does Coffee Have Anti-fungal Properties? [Re: mynakedrat]
    #24278136 - 04/28/17 08:13 AM (7 years, 8 months ago)

I thought you use coffee with oysters to boost nitrogen


--------------------

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InvisiblebodhisattaMDiscordReddit
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Re: Does Coffee Have Anti-fungal Properties? [Re: Kevlar123]
    #24278188 - 04/28/17 08:36 AM (7 years, 8 months ago)

Quote:

Kevlar123 said:
Hello,

The coffee was PC'd for 60 minutes, same as all of the other jars.  The only difference between them is that some have a few coffee beans added.  This for some reason seems to have slowed growth considerably.  The Myc is still growing on the coffee just much slower.



If you only pressure cooker 60 minutes there's your problem

Everything is contaminated anyway. Probably 50/50 your technique and then also inadequate sterilization


Looking up unrelated articles on scholar to try to back yourself up is confirmation bias at its finest

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OfflineKevlar123
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Re: Does Coffee Have Anti-fungal Properties? [Re: bodhisatta]
    #24278205 - 04/28/17 08:47 AM (7 years, 8 months ago)

Quote:

bodhisatta said:
Quote:

Kevlar123 said:
Hello,

The coffee was PC'd for 60 minutes, same as all of the other jars.  The only difference between them is that some have a few coffee beans added.  This for some reason seems to have slowed growth considerably.  The Myc is still growing on the coffee just much slower.



If you only pressure cooker 60 minutes there's your problem

Everything is contaminated anyway. Probably 50/50 your technique and then also inadequate sterilization


Looking up unrelated articles on scholar to try to back yourself up is confirmation bias at its finest





No need to be an arse mate, how is it confrontational bias? referencing is standard practice in science.  When controlling for the all the variables all the jars were PC'd for 60 minutes and ONLY the coffee ones have poor growth.  I quickly calculated P and its P = 0.0085, that coffee was the factor.

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OfflineKevlar123
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Re: Does Coffee Have Anti-fungal Properties? [Re: AndyHinton]
    #24278209 - 04/28/17 08:49 AM (7 years, 8 months ago)

Quote:

AndyHinton said:
No comparison is possible in this case because each jar is growing a different culture.

Coffee is a good microbe substrate in my experience; it molds over as fast as bread. It's also acidic and caffeinated, which are two undesirable qualities for mush cult.

I add the last espresso grounds to fruiting substrates. If anything, it gives traditional supplements like wheat bran a little kick.

For whatever reason, stones and oysters seem to really love it.




It was different spores that colonised each jar, but a comparison is definitely available, that's why statistics exist in science because you can't test under perfect conditions.

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InvisiblebodhisattaMDiscordReddit
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Re: Does Coffee Have Anti-fungal Properties? [Re: Kevlar123]
    #24278217 - 04/28/17 08:50 AM (7 years, 8 months ago)

Well searching for the answer you want to hear as a keyword is the worst way to research unbiased.

You can't calculate an honest P because there's no controls, and there's no experiment set up.

Since you insufficiently sterilized the coffee contaminated quickly. It has nothing to do with any anti fungal properties. Using coffee you have to be extra diligent

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Invisiblemynakedrat
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Re: Does Coffee Have Anti-fungal Properties? [Re: Kevlar123]
    #24278218 - 04/28/17 08:50 AM (7 years, 8 months ago)

How do you know exactly what spore grew where?
There are literally thousands of them, all with different Genetics.
You are looking to find a way to prove you are correct, that's all. Humans do that

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OfflineKevlar123
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Re: Does Coffee Have Anti-fungal Properties? [Re: mynakedrat]
    #24278223 - 04/28/17 08:53 AM (7 years, 8 months ago)

WTF, please don't pigeon hole me.  I made an observation that I've reported to the forum and I posted some papers that were inline with my observation.  Ad Hominem logical fallacies against myself don't change these observations.

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Shop: Mushroom-Hut Liquid Cultures   OlympusMyco.com No Unicorns Here—Just Quality Bags That Work   Left Coast Kratom Buy Kratom Extract   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Original Sensible Seeds Autoflowering Cannabis Seeds   North Spore Bulk Substrate   Myyco.com Isolated Cubensis Liquid Culture For Sale   MagicBag.co All-In-One Bags That Don't Suck   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Bridgetown Botanicals CBD Concentrates   Kraken Kratom Red Vein Kratom


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