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OfflineTrippyLivin
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Cheapest Agar Containers
    #24275266 - 04/27/17 08:31 AM (7 years, 8 months ago)

Pasty's agar tek/grocery store tek/ cheapest possible agar

Alright so I located 8 pack ziplock mini containers for like 2.50 each or something.


Very cheap and all locally obtained. I used agar-agar brick from local Asian store, as well as powdered laboratory grade agar, and standard telephone brand agar powder(my favorite and cheapest).

I drilled 1/4th inch holes into each lid twice. Once in the middle and once on the outside. Through trial and error I THINK that when I drilled the hole in the middle it caused about 4/24. 16% failure rate is not something to be proud of. Anywho the 4 that didn't work won't let the lid clip down over the tyvek. All the rest are awesome. The ones that wouldn't "work" only 3/4 corners would clip down on the plastic container. 1 would just refuse to go.



Some I put the RTV in the middle like the picture below. Sometimes I put it on the side to test what would work best. I also made an effort to fill the open area between lid and tyvek with RTV as you can see. I knew the RTV doesn't like to grip to plastic unless I sandpaper it which I was feeling lazy but it seemed to work. Any recommendations on how to lower my failure rate? I'm gonna make 100 once I feel comfortable with the method.



I can get Petri dishes local 12$ for 10 large I believe. Glass mason jars are awesome but pricey when I wanna do large scale agar experiments. I have 6 different prints, different strains, I'd like to isolate a sector from. I injected 2-3 drops of LC into each agar container. Shouldn't be long now :smile: I still have maybe 200 CC of LC in 35 CC syringes left. Just tryna get rid of it cause it seems the syringes I put it in don't hold the liquid in very well...I just wanted bigger syringes


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Happy trippin!

-TrippyLivin

Check out my indoor B+ and Golden Teacher Indoor Bulk Grow!
Adding AA+ Albino Cubensis,  Corumba Brazil Cubensis, Mazatapec Cubensis, and Texas PE6 Cubensis

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OfflineCapnZ
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Re: Cheapest Agar Containers [Re: TrippyLivin]
    #24275344 - 04/27/17 09:09 AM (7 years, 8 months ago)

I like using Petrie doses, easier to store. Here's a good place to get them at a good price:

http://www.carolina.com/lab-dishes/polystyrene-disposable-sterile-petri-dishes/FAM_741246.pr


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Deep into the darkness peering, long I stood there wondering, fearing, doubting, dreaming dreams no mortal ever dared to dream before...

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OfflinehamloafM
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Re: Cheapest Agar Containers [Re: CapnZ]
    #24275350 - 04/27/17 09:12 AM (7 years, 8 months ago)

Cynmar.com is much cheaper than those guys.  Cynmar sells their petris in packs of 25 too.


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OfflineCapnZ
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Re: Cheapest Agar Containers [Re: hamloaf]
    #24275353 - 04/27/17 09:13 AM (7 years, 8 months ago)

Quote:

hamloaf said:
Cynmar.com is much cheaper than those guys.  Cynmar sells their petris in packs of 25 too.



Awesome thanks!


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Deep into the darkness peering, long I stood there wondering, fearing, doubting, dreaming dreams no mortal ever dared to dream before...

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OfflineTrippyLivin
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Re: Cheapest Agar Containers [Re: CapnZ]
    #24275355 - 04/27/17 09:14 AM (7 years, 8 months ago)

That's roughly the same price and mine are reusable.


--------------------
Happy trippin!

-TrippyLivin

Check out my indoor B+ and Golden Teacher Indoor Bulk Grow!
Adding AA+ Albino Cubensis,  Corumba Brazil Cubensis, Mazatapec Cubensis, and Texas PE6 Cubensis

Starting Greenhouse Grow Bulk

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InvisiblebodhisattaMDiscordReddit
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Re: Cheapest Agar Containers [Re: TrippyLivin]
    #24275361 - 04/27/17 09:17 AM (7 years, 8 months ago)

Yea but reusable is more work. Try cleaning out 100 dishes

I put three bottles of agar in my PC and then pour 60 plates
How many PC cycles to get enough plates to put 6 varieties of spores to plates?

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InvisiblemushboyMDiscord
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Re: Cheapest Agar Containers [Re: CapnZ]
    #24275365 - 04/27/17 09:18 AM (7 years, 8 months ago)

100 minis is a shit load.

this is a bitch and this is what? 25-30??


trying to do 6 different isolates would probably take about 300 transfers. at least. so id go with
pouring your own dishes. a box of 500 petris is 80bucks. shop around.

mini rounds warp after a few cycles too. making the lids kinda worthless unless you tape them down..
as for filters.. just use MP tape. that injection port shit is noobish and pointless. just use 1 hole. 2 layers or tape.
you pop the lids open to do transfers. you only need 1 plate to germ spores on. dont mod hundreds of lids like that:facepalm:

whats with the red striped stuff under the lid in the pics?
you have THAT MUCH LC? how did you make it?:uhoh:

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InvisibleAndyHinton


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Re: Cheapest Agar Containers [Re: mushboy] * 1
    #24275428 - 04/27/17 09:43 AM (7 years, 8 months ago)

I like the 2 oz jelly jars. They're $10 a dozen, reusable forever, and the boxes stack neatly. Rotating among 5-6 dozen plates is no problem. I always have sterile, ported plates on hand. Being able to grab and inject plates on a whim just makes everything so much easier for me.

No lab waste, either. Plastic petris consume 3 squares of Parafilm every time you open them (1.5 squares if cut lengthwise), and you also have to dispose of the petris. It takes 3 seconds to wash a tiny jar.


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OfflineTrippyLivin
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Re: Cheapest Agar Containers [Re: mushboy]
    #24277417 - 04/27/17 10:21 PM (7 years, 8 months ago)

Quote:

mushboy said:
100 minis is a shit load.

this is a bitch and this is what? 25-30??


trying to do 6 different isolates would probably take about 300 transfers. at least. so id go with
pouring your own dishes. a box of 500 petris is 80bucks. shop around.

mini rounds warp after a few cycles too. making the lids kinda worthless unless you tape them down..
as for filters.. just use MP tape. that injection port shit is noobish and pointless. just use 1 hole. 2 layers or tape.
you pop the lids open to do transfers. you only need 1 plate to germ spores on. dont mod hundreds of lids like that:facepalm:

whats with the red striped stuff under the lid in the pics?
you have THAT MUCH LC? how did you make it?:uhoh:




The red stripes is from tyvek envelopes. Never tried it but figured I'd go for it. I have SFD. Can you just cut them into smaller pieces since it's so pricey or stick with the tape? I have polyfil but I hate it
Well I have 4 pint mason jars of corn syrup/water with magnetic stir rods.
I have 300 CC of sterilized water I injected into a beyond clean colonized rye jar. Shook the shit outta it then sucked it back up. Tested it multiple times. Basically using dunk water to do it. One quart jar makes 8 35cc syringes full of it. And each syringe literally is full of white long mycelium strands


--------------------
Happy trippin!

-TrippyLivin

Check out my indoor B+ and Golden Teacher Indoor Bulk Grow!
Adding AA+ Albino Cubensis,  Corumba Brazil Cubensis, Mazatapec Cubensis, and Texas PE6 Cubensis

Starting Greenhouse Grow Bulk

Edited by TrippyLivin (04/27/17 10:23 PM)

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InvisibleTheMadHatter420
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Registered: 10/12/16
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Re: Cheapest Agar Containers [Re: mushboy]
    #24277450 - 04/27/17 10:41 PM (7 years, 8 months ago)

Quote:

mushboy said:
trying to do 6 different isolates would probably take about 300 transfers. at least.





I now have 8 cube variety and one exotic cleaning on agar. I'm kicking myself in the ass, cause it's a pain in the ass, but hey not going to toss them now, just never going to clean more that 2-3 at a time.


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OfflineTrippyLivin
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Re: Cheapest Agar Containers [Re: TheMadHatter420]
    #24277466 - 04/27/17 10:53 PM (7 years, 8 months ago)

Quote:

TheMadHatter420 said:
Quote:

mushboy said:
trying to do 6 different isolates would probably take about 300 transfers. at least.





I now have 8 cube variety and one exotic cleaning on agar. I'm kicking myself in the ass, cause it's a pain in the ass, but hey not going to toss them now, just never going to clean more that 2-3 at a time.




I guess I shouldn't of ordered so many prints... you know it's funny cause I complained they were too small. This batches prints are so big idk what the fuck do with all of it. I made 100 12CC syringes in one night. I only finished 4 prints. Each syringe is so thick you can see it


--------------------
Happy trippin!

-TrippyLivin

Check out my indoor B+ and Golden Teacher Indoor Bulk Grow!
Adding AA+ Albino Cubensis,  Corumba Brazil Cubensis, Mazatapec Cubensis, and Texas PE6 Cubensis

Starting Greenhouse Grow Bulk

Extras: Filter Print Post Top
InvisibleTheMadHatter420
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Registered: 10/12/16
Posts: 12,943
Re: Cheapest Agar Containers [Re: TrippyLivin]
    #24277514 - 04/27/17 11:27 PM (7 years, 8 months ago)

IMO don't put them into a syringe unless your going to use in a couple weeks. I feel the water in the syringe allows bacteria on the spores to breed. I like to keep a print until closer to use.


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InvisiblebodhisattaMDiscordReddit
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Re: Cheapest Agar Containers [Re: TheMadHatter420]
    #24277520 - 04/27/17 11:33 PM (7 years, 8 months ago)

Nah bacteria needs food to grow.

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InvisibleTheMadHatter420
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Registered: 10/12/16
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Re: Cheapest Agar Containers [Re: bodhisatta]
    #24277521 - 04/27/17 11:34 PM (7 years, 8 months ago)

I have seen bits of gill tissue and veil tissue on prints. Would this not, should some get in there, feed some bacteria, or and I just paranoid?


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Invisiblec10h12n2o
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Re: Cheapest Agar Containers [Re: TheMadHatter420]
    #24277605 - 04/28/17 12:34 AM (7 years, 8 months ago)

buying plates by the case comes out around $0.10 per plate, cant beat that

no way i could do 200 pp5 plates in a day, but with disposable plates its totally doable


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