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Invisiblec10h12n2o
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Surface Sterilizing Before Cloning
    #24274874 - 04/27/17 01:14 AM (7 years, 8 months ago)

hello lovely people

so i just read something really cool in a lab manual about mycorrhizae and other plant/fungus relationships

in the manual, they take plant samples and then culture various fungi present in the tissues

when they got the samples back to the lab, they held them in a bunsen burner and "surface sterilized" them before splitting them open and taking culture samples

this reminded me of the way we used to see people recommending the use of peroxide for cloning (though of course it is now accepted as causing more harm than good)

of course a flame will probably damage the tissue even more than peroxide would lol.... but it seems like it would be a really good technique for killing off surface contams quite effectively

obviously this isnt really needed, since its so damned easy to get a clean clone culture in the first place, but it seems to be of use to researchers especially in cases where there will be a huge range of surface contams

anyways, just wanted to share, since the technique could easily be adapted for our purposes, perhaps in the caase of a particularly dirty fruit to be cloned, etc


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OfflineKingdeviluke
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Re: Surface Sterilizing Before Cloning [Re: c10h12n2o]
    #24274968 - 04/27/17 03:00 AM (7 years, 8 months ago)

Neat

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Offlinemushhiehunter
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Re: Surface Sterilizing Before Cloning [Re: Kingdeviluke]
    #24275068 - 04/27/17 05:52 AM (7 years, 8 months ago)

Yeah I don't see any problem with it, m9st likely only a couple of milimeters get burned this way and you are taking the sample from the middle which will stay at the same temp unless you really keep the stem in the flame for a looong time..

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InvisiblemushboyMDiscord
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Re: Surface Sterilizing Before Cloning [Re: mushhiehunter]
    #24275114 - 04/27/17 06:40 AM (7 years, 8 months ago)

Flame sterilizing tissue eh? I wonder what burning shroom matter smells like.

Cool.:cool:

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InvisibleAndyHinton


Registered: 12/05/16
Posts: 434
Re: Surface Sterilizing Before Cloning [Re: mushboy]
    #24275167 - 04/27/17 07:34 AM (7 years, 8 months ago)

It's common to soak plant tissue in 10% bleach before plating it out. Do you have a link or citation for the lab manual? I'd like to try my draft mycorrhizae tek soon and need all the knowledge I can get.

The goal is to stimulate metabolic pathways as the plant roots differentiate and the mycorrhizal spores germinate. If it works I'll try a chanterelle tek. A knowledgeable member told me that my proposed method has already been done. :smile:


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Invisiblemynakedrat
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Re: Surface Sterilizing Before Cloning [Re: mushboy]
    #24275254 - 04/27/17 08:27 AM (7 years, 8 months ago)

Quote:

mushboy said:
Flame sterilizing tissue eh? I wonder what burning shroom matter smells like.

Cool.:cool:



You've never smoked Em?
Thays the only Way to fly man, haven't ya heard?

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OfflineCapnZ
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Re: Surface Sterilizing Before Cloning [Re: mynakedrat]
    #24275349 - 04/27/17 09:11 AM (7 years, 8 months ago)

Quote:

mynakedrat said:
Quote:

mushboy said:
Flame sterilizing tissue eh? I wonder what burning shroom matter smells like.

Cool.:cool:



You've never smoked Em?
Thays the only Way to fly man, haven't ya heard?




Hmmm have to try that!

Any thoughts on dwell time in the flame? Quick pass?


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InvisiblebodhisattaMDiscordReddit
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Re: Surface Sterilizing Before Cloning [Re: CapnZ]
    #24275352 - 04/27/17 09:13 AM (7 years, 8 months ago)

Dip it in 91 then light it up lol.
I've never had a hard time cloning but if I did i would try iodine first. Iodophor based sanitizer

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OfflineCapnZ
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Re: Surface Sterilizing Before Cloning [Re: bodhisatta]
    #24275407 - 04/27/17 09:35 AM (7 years, 8 months ago)

Quote:

bodhisatta said:
Dip it in 91 then light it up lol.
I've never had a hard time cloning but if I did i would try iodine first. Iodophor based sanitizer



Cool. I have some of that sanitizer. Home brew store!


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Deep into the darkness peering, long I stood there wondering, fearing, doubting, dreaming dreams no mortal ever dared to dream before...

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