|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
|
Surface Sterilizing Before Cloning
#24274874 - 04/27/17 01:14 AM (7 years, 8 months ago) |
|
|
hello lovely people
so i just read something really cool in a lab manual about mycorrhizae and other plant/fungus relationships
in the manual, they take plant samples and then culture various fungi present in the tissues
when they got the samples back to the lab, they held them in a bunsen burner and "surface sterilized" them before splitting them open and taking culture samples
this reminded me of the way we used to see people recommending the use of peroxide for cloning (though of course it is now accepted as causing more harm than good)
of course a flame will probably damage the tissue even more than peroxide would lol.... but it seems like it would be a really good technique for killing off surface contams quite effectively
obviously this isnt really needed, since its so damned easy to get a clean clone culture in the first place, but it seems to be of use to researchers especially in cases where there will be a huge range of surface contams
anyways, just wanted to share, since the technique could easily be adapted for our purposes, perhaps in the caase of a particularly dirty fruit to be cloned, etc
--------------------
 
C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide
"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."
"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche
|
Kingdeviluke
Stranger

Registered: 04/26/17
Posts: 327
Last seen: 7 years, 6 months
|
Re: Surface Sterilizing Before Cloning [Re: c10h12n2o]
#24274968 - 04/27/17 03:00 AM (7 years, 8 months ago) |
|
|
Neat
|
mushhiehunter
Trichodermatologist



Registered: 10/11/16
Posts: 837
Loc: Česká republika
Last seen: 7 months, 12 days
|
Re: Surface Sterilizing Before Cloning [Re: Kingdeviluke]
#24275068 - 04/27/17 05:52 AM (7 years, 8 months ago) |
|
|
Yeah I don't see any problem with it, m9st likely only a couple of milimeters get burned this way and you are taking the sample from the middle which will stay at the same temp unless you really keep the stem in the flame for a looong time..
|
mushboy
modboy



Registered: 04/24/05
Posts: 35,378
Loc: eating the cats
|
Re: Surface Sterilizing Before Cloning [Re: mushhiehunter]
#24275114 - 04/27/17 06:40 AM (7 years, 8 months ago) |
|
|
Flame sterilizing tissue eh? I wonder what burning shroom matter smells like.
Cool.
|
AndyHinton


Registered: 12/05/16
Posts: 434
|
Re: Surface Sterilizing Before Cloning [Re: mushboy]
#24275167 - 04/27/17 07:34 AM (7 years, 8 months ago) |
|
|
It's common to soak plant tissue in 10% bleach before plating it out. Do you have a link or citation for the lab manual? I'd like to try my draft mycorrhizae tek soon and need all the knowledge I can get.
The goal is to stimulate metabolic pathways as the plant roots differentiate and the mycorrhizal spores germinate. If it works I'll try a chanterelle tek. A knowledgeable member told me that my proposed method has already been done.
--------------------
|
mynakedrat
The phantom hourglass



Registered: 02/16/17
Posts: 4,629
Loc: Inner Astral levels
|
Re: Surface Sterilizing Before Cloning [Re: mushboy]
#24275254 - 04/27/17 08:27 AM (7 years, 8 months ago) |
|
|
Quote:
mushboy said: Flame sterilizing tissue eh? I wonder what burning shroom matter smells like.
Cool.
You've never smoked Em? Thays the only Way to fly man, haven't ya heard?
|
CapnZ
Dimensional explorer



Registered: 12/22/16
Posts: 1,420
Loc: In the Mystic
Last seen: 1 year, 3 months
|
Re: Surface Sterilizing Before Cloning [Re: mynakedrat]
#24275349 - 04/27/17 09:11 AM (7 years, 8 months ago) |
|
|
Quote:
mynakedrat said:
Quote:
mushboy said: Flame sterilizing tissue eh? I wonder what burning shroom matter smells like.
Cool.
You've never smoked Em? Thays the only Way to fly man, haven't ya heard?
Hmmm have to try that!
Any thoughts on dwell time in the flame? Quick pass?
-------------------- Deep into the darkness peering, long I stood there wondering, fearing, doubting, dreaming dreams no mortal ever dared to dream before...
|
bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,915
Loc: Milky way
|
Re: Surface Sterilizing Before Cloning [Re: CapnZ]
#24275352 - 04/27/17 09:13 AM (7 years, 8 months ago) |
|
|
Dip it in 91 then light it up lol. I've never had a hard time cloning but if I did i would try iodine first. Iodophor based sanitizer
|
CapnZ
Dimensional explorer



Registered: 12/22/16
Posts: 1,420
Loc: In the Mystic
Last seen: 1 year, 3 months
|
Re: Surface Sterilizing Before Cloning [Re: bodhisatta]
#24275407 - 04/27/17 09:35 AM (7 years, 8 months ago) |
|
|
Quote:
bodhisatta said: Dip it in 91 then light it up lol. I've never had a hard time cloning but if I did i would try iodine first. Iodophor based sanitizer
Cool. I have some of that sanitizer. Home brew store!
-------------------- Deep into the darkness peering, long I stood there wondering, fearing, doubting, dreaming dreams no mortal ever dared to dream before...
|
|
|
You cannot start new topics / You cannot reply to topics HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a 397 topic views. 24 members, 112 guests and 13 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ] |
|