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Offlinejusthavefun
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Registered: 03/02/17
Posts: 87
Last seen: 2 years, 8 months
Agar contamination
    #24270476 - 04/25/17 11:14 AM (7 years, 8 months ago)

Hey guys,
i just started off with agar some weeks ago and prepared half a dozen pour agar plates. I inoculated 2 of them with MS Golden Teacher, fucked up two by dumbness and sealed the other two and stored them at room temp in order to check my sterile technique.
The myc grew quite decent, but in one some contam popped up. Did one transfer from both of the myc plates (to fresh made no-pour), no contams anymore (yet). Second transfer has been done today.
However, after 5 days i checked my control plates and they were clean, another 3-4 days later i checked again and there were 1/2 really small contam spot.
Is it normal that contams take that long to show ab? I only use steam sterilization (45min) at atmospheric pressure, might that cause some heat resistant spore etc. to survive? Or did i just make some mistake during pouring?
No pour plates (glass petri) dishes are, as mentioned before, perfectly fine. However, they only have been inside the incubation chamber for round about 6 days, so i guess mycelium had quite an edge anyway.
Thanks for tips guys, and to all noobs (likes me :laugh:):
Agar is really fun and cheap af (spent like 15$ and was ready to go) i recommend trying it out!

Edit: Ill add some pictures as soon as im home.


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Looking for a Penis Envy Spore Syringe (paying BTC or trading for GT print)

Edited by justhavefun (04/25/17 11:15 AM)

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InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
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Registered: 04/30/13
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Re: Agar contamination [Re: justhavefun]
    #24270504 - 04/25/17 11:36 AM (7 years, 8 months ago)

it can take 7+ days for some contaminants to germinate. also some contaminants may not germinate until some criteria is met like temperature or ph change etc..

any single contaminant on a place means that there's the possibility that any other contaminant could have got in. so check your techniques

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Offlinejusthavefun
Stranger

Registered: 03/02/17
Posts: 87
Last seen: 2 years, 8 months
Re: Agar contamination [Re: bodhisatta]
    #24272254 - 04/26/17 04:24 AM (7 years, 8 months ago)

Thanks bod! Will try to improve next time.


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Looking for a Penis Envy Spore Syringe (paying BTC or trading for GT print)

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InvisiblePhibonacci
Stranger in a strange land

Registered: 05/25/16
Posts: 50
Re: Agar contamination [Re: justhavefun]
    #24273461 - 04/26/17 02:36 PM (7 years, 8 months ago)

Save a couple plates for controls. If they don't contam then the contam was post-agar pour

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