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Offlineelectricvelvet
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Super fluffy myc on agar? Not mold...
    #24268457 - 04/24/17 01:03 PM (7 years, 8 months ago)

K so to make things brief I had a 6 month old colonized grain jar that I'd never used or opened. There were metabolites at the bottom so as an experiment I used a sterile syringe to draw up some of those metabolites assuming some live myc would be in the solution. I put some drops on a pasty plate & injected the rest into a half pint grain jar.

It bears mentioning that the myc of that golonized jar was also very thick and fluffy, and definitely p cube myc because it never changed colors or anything like that.

The growth on the plate looks exactly like the jar's myc. But every time I come here everyone says the same thing, there's something unhealthy or wrong if the myc is cottony and not rhyzomorphic. Some suggested bacterial contam but now that I have it on a plate it is evident that this myc is the only organism growing on the plate (unless the bacteria is somehow growing on top of the myc? Is that possible?)

Without further ado here's the pictures. Some of them suck but I took a ton so maybe one will give you a good look.

What's up with the myc? Does anyone have an explanation? Is it viable or is it fucked, and if it's the latter, what's wrong with it?

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InvisibleTheMadHatter420
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Re: Super fluffy myc on agar? Not mold... [Re: electricvelvet] * 1
    #24268468 - 04/24/17 01:12 PM (7 years, 8 months ago)

realistically you should have sucked up some bacteria to. I would have used clean tweezers and picked a colonized grain from the top. If the whole jar was just a mushy pile of decomposing waste, well I wouldn't have messed with it, BUT if it's just experimenting then hey why not, we learn from failures so sometimes you go to do some thing you know will fail, or have a high chance at it, so your able to understand why it fails. I wouldn't think it would be mold unless you were able to see mold growing in that jar. My KSSS looks like fucking mold, I know it's not because I been doing transfers with it on agar for over a month and no discoloration. If it is mold, it will have a fairly uniform growth and will throw up its colors quick.


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Offlinemushpunx
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Re: Super fluffy myc on agar? Not mold... [Re: electricvelvet]
    #24268471 - 04/24/17 01:15 PM (7 years, 8 months ago)

Too hard to see, take a photo with the top open in SAB

I would have forked a grain with a sterile scalpel to drop on the plate and

Metabolites are not normal, they're only present when the mycelium is stressed , usually because of bacteria.

Take a transfer from the best looking spot on the plate and transfer it, see how it does


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InvisibleTheMadHatter420
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Re: Super fluffy myc on agar? Not mold... [Re: mushpunx]
    #24268476 - 04/24/17 01:17 PM (7 years, 8 months ago)

Was this just to see what would come of it, or are you like out of spores and trying to keep a culture going so you have something to work with?


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Offlineelectricvelvet
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Re: Super fluffy myc on agar? Not mold... [Re: TheMadHatter420]
    #24268486 - 04/24/17 01:29 PM (7 years, 8 months ago)

The jars were really old, the spawn was shrinking away from the sides of the jar. As the nutrients are consumed over time in a jar that's left alone after fully colonizing metabolites are naturally a byproduct. So myc piss is NOT a guarantee of bacterial contam. And yes It was more an experiment to see if myc would grow from the metabolites (obviously not FROM the metabolites but from the bits of myc in the metabolite solution).

Whenever I've had contaminated plates with bacteria, the myc and bacterial colony would NOT overlap. They'd form a border. So, can bacteria grow literally on top of or between the myc?

I have other cultures and spores. This is for my own knowledge. However it would be cool to salvage it because it's PE#6. Penis envy, I'll remind you, does grow slower and thicker typically than other strains of cube. Is my answer as simple as that?

I'll definitely make a transfer but I was just wondering if anyone had any input on why possibly the myc is like this. Bacteria, strain genetics, etc...

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Offlinemushpunx
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Re: Super fluffy myc on agar? Not mold... [Re: electricvelvet]
    #24268713 - 04/24/17 03:37 PM (7 years, 8 months ago)

There is no such thing as "myc piss", It is not simply a byproduct of mycelium. Mycelium produces metabolites in response to contamination or stress. There are anti biotic compounds in the metabolites that are there to try to fight bacteria or competitor fungi.

Myc can overlap bacteria yes. That's why its important to take transfers from myc furthest from bacteria on a plate before that happens.



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Edited by mushpunx (04/24/17 03:55 PM)

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