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simple_simo
Clit Commander



Registered: 07/28/10
Posts: 67
Loc: Sydney
Last seen: 1 year, 5 months
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Help with subaeruginosa substrate choice.
#24266951 - 04/23/17 06:33 PM (7 years, 8 months ago) |
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Hey guys, long time reader but few and far beyond in the posting division. I have a question regarding choice of substrate when transferring P.subaeruginosa from cardboard onto woodchip beds.
Ive been doing it with lots of success over the years, make the cardboard, find a huge mulch pile and bag some up then take and spread the colonised cardboard into the woodchips gathered and make a new bed. However last year i got mulch delivered to my house after a wild storm, and the mulch was just cut down days ago and still steaming.
What i want to know, because i have read over the years that you should use woodchips that were cut down many months to a year before trying to colonise onto them chips. Is this true? and if so what is the science behind it? Like i said, ive always gotten mulch off piles that could have sat there for months or a year but i don't know the date since they were chipped, and the mulch i used last year which was all eucalypt species hasn't done so well for my upcoming patches..
Thank you all for your time in advance
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I love tits so much i grew a pair...
Seen a fat Aussie bloke wearing a t-shirt saying: I couldn't climb ayres rock. SO i ate it.
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TheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,943
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Re: Help with subaeruginosa substrate choice. [Re: simple_simo]
#24267015 - 04/23/17 07:14 PM (7 years, 8 months ago) |
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Well I do know that fresh cut mulch is bad for plant, especially boxwood. It pulls the nitrogen out of the ground and will cause leaves to turn green and can kill plants. My hort. professor told the class to never use them for plants because of that. Later that spring I delivered some pizzas to an apartment building where they did that, man the boxwoods were fucked, they died in a couple days. Not sure if it can do similar thing to shrooms or not. I would assume it would be harder for them to break the fresh chips down.
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simple_simo
Clit Commander



Registered: 07/28/10
Posts: 67
Loc: Sydney
Last seen: 1 year, 5 months
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Re: Help with subaeruginosa substrate choice. [Re: TheMadHatter420]
#24267029 - 04/23/17 07:25 PM (7 years, 8 months ago) |
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Thanks, and yes, im a horticulturalist with 15 years experience. That is called nitrogen drawdown. Im just wondering how it plays into my chances of colonisation tho.. Ive always had great success till this year, it seems most of my patches i topped up with fresh mulch havent done so well, and we are only a month out from fruiting here in australia..
Ive also read that nitrogen is important for mushroom/mycellium growth. Thanks for the response, i will definitely be looking into that as i continue my research. This is a question that has plagued me for many years, although is only becoming apparent my need for an answer this shroom season 
Cheers madhatter, and anyone else who might see what im missing feel free to butt in and give me some more advice
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I love tits so much i grew a pair...
Seen a fat Aussie bloke wearing a t-shirt saying: I couldn't climb ayres rock. SO i ate it.
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TheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,943
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Re: Help with subaeruginosa substrate choice. [Re: simple_simo]
#24267093 - 04/23/17 07:57 PM (7 years, 8 months ago) |
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Say how hard would it be to work out a deal for a print off one of those, like I have no idea what Australian customs is like for us to try and do a trade.
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simple_simo
Clit Commander



Registered: 07/28/10
Posts: 67
Loc: Sydney
Last seen: 1 year, 5 months
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Re: Help with subaeruginosa substrate choice. [Re: TheMadHatter420]
#24267128 - 04/23/17 08:12 PM (7 years, 8 months ago) |
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Hit me up in PM, and we can talk more there
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I love tits so much i grew a pair...
Seen a fat Aussie bloke wearing a t-shirt saying: I couldn't climb ayres rock. SO i ate it.
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Adden

Registered: 06/04/03
Posts: 39,201
Loc:
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Re: Help with subaeruginosa substrate choice. [Re: TheMadHatter420]
#24267596 - 04/24/17 12:42 AM (7 years, 8 months ago) |
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Quote:
TheMadHatter420 said: Say how hard would it be to work out a deal for a print off one of those, like I have no idea what Australian customs is like for us to try and do a trade.
Print on wax paper and tape it inside a greeting card.
Edit: Or just print on paper. I swabbed spores off a map sitting unprotected in my backpack for 2 years and got them to germ. If I can do it anyone can.
Edited by Adden (04/24/17 01:03 AM)
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Adden

Registered: 06/04/03
Posts: 39,201
Loc:
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Re: Help with subaeruginosa substrate choice. [Re: simple_simo]
#24267610 - 04/24/17 12:53 AM (7 years, 8 months ago) |
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Quote:
simple_simo said: Hey guys, long time reader but few and far beyond in the posting division. I have a question regarding choice of substrate when transferring P.subaeruginosa from cardboard onto woodchip beds.
Ive been doing it with lots of success over the years, make the cardboard, find a huge mulch pile and bag some up then take and spread the colonised cardboard into the woodchips gathered and make a new bed. However last year i got mulch delivered to my house after a wild storm, and the mulch was just cut down days ago and still steaming.
What i want to know, because i have read over the years that you should use woodchips that were cut down many months to a year before trying to colonise onto them chips. Is this true? and if so what is the science behind it? Like i said, ive always gotten mulch off piles that could have sat there for months or a year but i don't know the date since they were chipped, and the mulch i used last year which was all eucalypt species hasn't done so well for my upcoming patches..
Thank you all for your time in advance 
Keep doing what you're doing. Just avoid heavy amounts of pine or pine needles. Newer chips are harder yes but add a lasting and fresh food source.
What I found best was having a porch bucket discard bin. I put a bit of sandy soil collected around patches, mixed in maple, rhododendron and holly leaves up in it, and fed it butts and random chips sticks twigs. I made a few sheets of cardboard from butts and turned it into a slurry. This sped thngs up rapidly. It's now spawned into a park that chipped them. Honestly the best thing was just leaving them out on their own. I did use discarded agar and LC when it seemed like things were slowing down. At one point I just blended leaves, colonized cardboard and some agar pucks into a slurry (using a few sheets of spore print water as a base) and fed it even more.
I don't know what leaves or woods to suggest for you guys but birch, apple, maple, pine, tulip tree, cottonwood, shredded blackberry or raspberries, alder and extremely aged and washed pine are great for them. It's too acidic and the sap itself is anti fungal. Some people have gone so far as wash and wash and wash again with boiling water and someone here PC'd some after leeching them out. He grows awesome beds.
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NothingsChanged
Striving for Excellence



Registered: 05/28/11
Posts: 10,674
Loc: North/Western WA
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Re: Help with subaeruginosa substrate choice. [Re: Adden]
#24267644 - 04/24/17 01:22 AM (7 years, 8 months ago) |
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You don't want to use any thing if its almost fruiting season. would most likely shift back into colonization mode.
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simple_simo
Clit Commander



Registered: 07/28/10
Posts: 67
Loc: Sydney
Last seen: 1 year, 5 months
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Re: Help with subaeruginosa substrate choice. [Re: NothingsChanged]
#24269100 - 04/24/17 06:47 PM (7 years, 8 months ago) |
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Quote:
NothingsChanged said: You don't want to use any thing if its almost fruiting season. would most likely shift back into colonization mode.
Thanks for that info Adden, very useful to what im doing.
And nothingschanged, im talking about next years beds that im making now. I used all the fresh mulch last year and most of my beds about to fruit have showed significant drop in colonisation as a result. I'm trying not to make the same mistake on next years beds now that im preparing, as i don't think i'm going to get the results i have been the past 8 years. Ive always wondered this sort of stuff when i read about using fresh woodchips to colonise many many years ago, but with so much success in the past it just lingered in my head. Now its actually become reality and im trying to understand better, the process i just seemed to have gotten lucky with for so long. But thank you for your input, i do appreciate anyone who has taken the time to help.
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I love tits so much i grew a pair...
Seen a fat Aussie bloke wearing a t-shirt saying: I couldn't climb ayres rock. SO i ate it.
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Adden

Registered: 06/04/03
Posts: 39,201
Loc:
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Re: Help with subaeruginosa substrate choice. [Re: simple_simo]
#24269292 - 04/24/17 08:08 PM (7 years, 8 months ago) |
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When they're fruiting they'll slow down on colonization. The only exception I've seen are ovoids which you guys don't have, and those love colonizing as they fruit.
Someone here expands cardboard to cardboard to more cardboard and ends up cutting open boxes to lay down before spawning a bed. They haven't been active lately I'll send a message and link this thread.
If you know how to do agar you can load up on LC and just water the things with it. Or just do agar to grains and sandwich grains between cardboard, lay it down as a mat, mix the base layer with colonized grains, do chips and grains and then top it off with chips. The fresher the chips the longer they'll last but native soils and stuff growing around where you pick is gonna be the best thing you can do.
Edited by Adden (04/24/17 08:17 PM)
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NothingsChanged
Striving for Excellence



Registered: 05/28/11
Posts: 10,674
Loc: North/Western WA
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Re: Help with subaeruginosa substrate choice. [Re: Adden]
#24269662 - 04/24/17 11:42 PM (7 years, 8 months ago) |
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When ever i use older chips they seem to beloaded with other fungi that takes over. I also notice with my fresh chips, the bark seems to get the first signs of life. Every thing ive read says not to use the bark. Now i use fresh wood but a mix of saw dust,shredded,chunks,chips and everything in between.
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