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OfflineLove_spirit
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Worst cultivation ideas perpetuated by this site
    #24263712 - 04/22/17 10:12 AM (7 years, 8 months ago)

A few Ideas that should be banished.

1. The PF Tek.

2. Liquid Cultures

3. Using coffee grounds

4. Using paint suits as filters

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InvisiblemushboyMDiscord
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Re: Worst cultivation ideas perpetuated by this site [Re: Love_spirit]
    #24263715 - 04/22/17 10:13 AM (7 years, 8 months ago)

Quote:

Love_spirit said:
A few Ideas that should be banished.

1. The PF Tek.

2. Liquid Cultures

3. Using coffee grounds

4. Using paint suits as filters

5. love_spirit




stop hatin':oldman:

besides coffee, ive used and have had great results with the others.

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InvisibleJust_A_Noob
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Re: Worst cultivation ideas perpetuated by this site [Re: Love_spirit]
    #24263717 - 04/22/17 10:14 AM (7 years, 8 months ago)

3 and 4 maybe...  PF tek is almost like a right of passage... LC?  Haven't had luck w/ LC I take it

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OfflineHierophant
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Re: Worst cultivation ideas perpetuated by this site [Re: Just_A_Noob]
    #24263727 - 04/22/17 10:18 AM (7 years, 8 months ago)

Liquid cultures are the shit


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InvisibleJvF
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Re: Worst cultivation ideas perpetuated by this site [Re: Love_spirit] * 1
    #24263736 - 04/22/17 10:25 AM (7 years, 8 months ago)

Ive had success with every single one of these things.

You jelly brah?


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Invisiblepetey mcenroe
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Re: Worst cultivation ideas perpetuated by this site [Re: JvF]
    #24263759 - 04/22/17 10:42 AM (7 years, 8 months ago)

I mostly done PF cakes because I don't need lots of shrooms. Plus since I live in the PNW, I used that to my advantage n made cyan beds outside so I don't even have to do anything to get shrooms. I used PF cakes as my spawn n my backyard is full of mycelium. Now all I have to do once a year is feed the beds which is a part of how I landscaped my yard n I'll have more than I'll ever need.


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Offlinemushpunx
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Re: Worst cultivation ideas perpetuated by this site [Re: JvF]
    #24263774 - 04/22/17 10:54 AM (7 years, 8 months ago)




You don't offer any explanations as to *why. Most experienced cultivators would disagree with you on these points.
Here's why I do :

I consider PF tek is a pretty clever method considering you can have 100% success rate without working with a clean culture. Spore syringes are almost never totally sterile , yet I have never lost a PF cake to contams

Liquid culture is great, provided it is done correctly. Spores to LC is what gives it a bad name.

I do liquid cultures two ways:

1. Simply blending an agar wedge in water in a sterile Eberbach or

2. Blending agar in eberbach container and adding to malt extract broth and spinning on a mag stir plate.

Nothing wrong with coffee grounds if they are properly pasteurized. I don't use them, but if you wanted to there is nothing wrong with it

And lastly...

Tyvek makes a great filter material. Painter suits are fine if you use a double layer, not to mention using tyvek sleeves in spawn bag prep.
What's wrong with it? Tyvek, polyfil... Both work really well as filter material.


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Edited by mushpunx (04/22/17 10:58 AM)

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OfflinehamloafM
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Re: Worst cultivation ideas perpetuated by this site [Re: mushpunx]
    #24263811 - 04/22/17 11:17 AM (7 years, 8 months ago)

Quote:

mushpunx said:
Liquid culture is great, provided it is done correctly. Spores to LC is what gives it a bad name.

I do liquid cultures two ways:

1. Simply blending an agar wedge in water in a sterile Eberbach or

2. Blending agar in eberbach container and adding to malt extract broth and spinning on a mag stir plate.




Number one isn't a liquid culture.  It's merely a method of transferring live mycelium culture using water as the culture transfer medium.  Liquid inoculant. 

A liquid culture is mycelium colonized in a nutrient rich liquid media broth as you've described in number 2. 

You are spot on about everything else though.


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Invisiblemynakedrat
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Re: Worst cultivation ideas perpetuated by this site [Re: hamloaf]
    #24263834 - 04/22/17 11:34 AM (7 years, 8 months ago)

1. Sterile teks
2. Says, what a joke
3. Anything that is grain. Hello?
4. Agar

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InvisibleMorelman
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Re: Worst cultivation ideas perpetuated by this site *DELETED* [Re: hamloaf]
    #24263881 - 04/22/17 11:49 AM (7 years, 8 months ago)

Post deleted by Morelman

Reason for deletion: Never again...



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OfflineCrispykoot
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Re: Worst cultivation ideas perpetuated by this site [Re: Morelman]
    #24263892 - 04/22/17 11:52 AM (7 years, 8 months ago)

Straight coir is a good substrate...


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InvisibleMad Season
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Re: Worst cultivation ideas perpetuated by this site [Re: Crispykoot]
    #24263907 - 04/22/17 11:57 AM (7 years, 8 months ago)

It is funny tho that I do literally none of those things in the op. Tho I did do LC for the longest time.. WITH AGAR.


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Offlinemushpunx
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Re: Worst cultivation ideas perpetuated by this site [Re: hamloaf]
    #24264007 - 04/22/17 12:39 PM (7 years, 8 months ago)

Quote:

"idunno said:
An LC, Or myc water? in your opinion, what are some of the pros and cons, of your decision  Oh sorry which is better for inoculation, of grains?



That's like asking if a melted ice cube is better than melted snow...

If mycelium is suspended in a liquid carrier, it's liquid culture, anyway you look at it.
RR"

I hear ya Ham, I should have made the distinction. I usually say liquid inoculant too but I lumped them together here because I think technically they both are liquid culture , at least in the Stamets books and what have you haha



Quote:

hamloaf said:
Quote:

mushpunx said:
Liquid culture is great, provided it is done correctly. Spores to LC is what gives it a bad name.

I do liquid cultures two ways:

1. Simply blending an agar wedge in water in a sterile Eberbach or

2. Blending agar in eberbach container and adding to malt extract broth and spinning on a mag stir plate.




Number one isn't a liquid culture.  It's merely a method of transferring live mycelium culture using water as the culture transfer medium.  Liquid inoculant. 

A liquid culture is mycelium colonized in a nutrient rich liquid media broth as you've described in number 2. 

You are spot on about everything else though.




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OfflinehamloafM
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Re: Worst cultivation ideas perpetuated by this site [Re: mushpunx]
    #24264963 - 04/22/17 08:12 PM (7 years, 8 months ago)

RR is incorrect.  Calling a liquid culture the same thing as a liquid inoculant is pure dogma. 

In Biology culture means:  the cultivation of bacteria, tissue cells (in ur case fungi), ect., in an artificial medium containing nutrients.  In it's action sense culture means:  maintaining bacteria, tissue cells (in our case fungi), ect., in conditions suitable for growth. 

It's as was stated above.  A technical liquid culture is culture that has been grown inside of a nutrient rich media broth. 

A liquid inoculant is a method using water as the medium to aspirate mycelium from either a grain culture, or agar culture, and transfer it to another substrate.  What the mycelium grows upon next will be the culture. 

RR is great,  Much respect is had for him, but that doesn't mean he' s right all the time.  Nobody is.  Furthermore, RR doesn't do LC so, RR trying to tell us what a liquid culture is like a drummer trying to teach someone to play guitar.


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Edited by hamloaf (04/23/17 04:40 AM)

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Offlinezajcob
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Re: Worst cultivation ideas perpetuated by this site [Re: hamloaf]
    #24265001 - 04/22/17 08:36 PM (7 years, 8 months ago)

I appreciate this explanation.  The distinctions you illuminated actually give a reason for having the two different cultivation terminologies in the first place.

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InvisibleJosex
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Re: Worst cultivation ideas perpetuated by this site [Re: zajcob] * 1
    #24265019 - 04/22/17 08:45 PM (7 years, 8 months ago)

I have yet to fuck it up using LC, if you know how to make it nice and clean, it is safer than most inoculation methods. PF tek is the bomb, and the combination of pf tek and clean LC is awesome.

OP, you and your weird noob posts are the ones who should be banished to a dark oblivion :cheers:

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Offlinemushpunx
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Re: Worst cultivation ideas perpetuated by this site [Re: Josex]
    #24265133 - 04/22/17 09:43 PM (7 years, 8 months ago)

Well then I stand corrected :shrug:
I'd seen mycelium suspension in water listed as Liquid Culture in some of the old Stamets books, and when I tried to confirm it with the search engine I saw RR repeatedly saying they were both called LCs so I just figured it must be some old school terminology.
It never actually came up until now because I've always made the distinction between LI/LC when talking about it :lol:


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InvisibleInocuole
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Re: Worst cultivation ideas perpetuated by this site [Re: Josex]
    #24265141 - 04/22/17 09:46 PM (7 years, 8 months ago)

Quote:

Josex said:
OP, you and your weird noob posts are the ones who should be banished to a dark oblivion :cheers:



:thatsayes:


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Re: Worst cultivation ideas perpetuated by this site [Re: Love_spirit]
    #24265171 - 04/22/17 10:10 PM (7 years, 8 months ago)

Quote:

Love_spirit said:
A few Ideas that should be banished.

1. The PF Tek.

2. Liquid Cultures

3. Using coffee grounds

4. Using paint suits as filters




i've seen pasty do some amazing Pf-tek cakes. So idk about that one. LC is usually for those using agar and have good technique down, and coffee grounds in a sub can be a good thing if pasteurized properly, and tyvek is good stuff if you don't have SFD's, MP tape, or poly available.


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