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OfflineTryna
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What do you think of my first agar?
    #24265083 - 04/22/17 09:12 PM (6 years, 11 months ago)

Got back into this after a year off, been having probs with my sterile technique. Started agar in the midst of these problems. Here is the before pic of the only plate that had growth( out of 4)




I grew that out for a few days and transferred 4 pieces to new plates and let this plate continue to grow out and this is what it looks like today.







How did I do? is there anything you guys can 'deduce' about my work looking at this plate? Does it look like it should?

Thanks!

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InvisiblemushboyMDiscord
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Re: What do you think of my first agar? [Re: Tryna]
    #24265123 - 04/22/17 09:39 PM (6 years, 11 months ago)

the first pic is bacterial as fuck.

and the others look nasty.
:whatdidyoudo:

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Offlinemushpunx
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Re: What do you think of my first agar? [Re: mushboy]
    #24265148 - 04/22/17 09:52 PM (6 years, 11 months ago)

Yea I agree.
In the first pic that scuzz all over the plate is bacteria , and you can see the myc responding to it in the subsequent plates.

I would start again with some fresh plates, inoculate with spore


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InvisibleBoogieman47
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Re: What do you think of my first agar? [Re: mushpunx]
    #24265354 - 04/23/17 01:05 AM (6 years, 11 months ago)

:whathesaid: you can try to sandwich those plates taking clean agar and dropping it over top in the chance clean myc grows through before the bacteria or atleast get a jump on the bacteria on the next plate but it may be easier for you to start over...

I assume you have a sab?? And are spraying that down with soapy water? Flaming the tip of your blade to red hot?? And wiping every plate your hands and arms down with 70%iso before starting?


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Invisiblec10h12n2o
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Re: What do you think of my first agar? [Re: Boogieman47]
    #24265438 - 04/23/17 02:01 AM (6 years, 11 months ago)

Way overgrown.. you want to transfer to a new plate before it gets that overgrown, and bacteria has time to spread throughout the colony

It's hard to distinguish characteristics and pick good samples to transfer when it gets that overgrown

I'd start over and streak the spores like this, then transfer a clean colony from zone 4 as soon as it shows up



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OfflineTryna
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Re: What do you think of my first agar? [Re: mushpunx]
    #24265601 - 04/23/17 05:36 AM (6 years, 11 months ago)

Quote:

mushpunx said:
Yea I agree.
In the first pic that scuzz all over the plate is bacteria , and you can see the myc responding to it in the subsequent plates.

I would start again with some fresh plates, inoculate with spore




how can you tell the myc is responding to bacteria here?

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Invisiblec10h12n2o
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Re: What do you think of my first agar? [Re: Tryna]
    #24265630 - 04/23/17 06:09 AM (6 years, 11 months ago)

As the kind gentlemen said, there is visible bacterial colonies on the first plate

And in the subsequent plates,aside from being overgrown, there appears to be visible bacteria as well. The myc is acting weird and growing unevenly in response


--------------------

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"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche

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OfflineTryna
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Re: What do you think of my first agar? [Re: c10h12n2o]
    #24265633 - 04/23/17 06:14 AM (6 years, 11 months ago)

:frown:  my technique was pretty bad in the SAB. Never had a prob with contaminated doing open air nocs, why start over, what happens if i continue growing these plates out? because I have 4 cultures that i took off this plate.

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OfflineTryna
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Re: What do you think of my first agar? [Re: Tryna]
    #24265635 - 04/23/17 06:16 AM (6 years, 11 months ago)

NM i saw some other posts that answer the question, thanks all

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InvisibleMad Season
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Re: What do you think of my first agar? [Re: Tryna] * 1
    #24265636 - 04/23/17 06:17 AM (6 years, 11 months ago)

Sab techniques were bad so your solution was to go and do work in open air??? Wtf??

Haven't you ever heard the line practice makes perfect?

If you were to drop those into jars, you're purposefully adding bacteria to the jars. Sounds like a good way to see trich in bulk sub, and a huge waste of time..


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Offlinemrmazdarx9
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Re: What do you think of my first agar? [Re: Mad Season]
    #24265638 - 04/23/17 06:21 AM (6 years, 11 months ago)

Wow ive never seen such bad ratings you must of pissed some people off.
Anyway back on topic those plates look nasty you could try make transfers from them but it would be easier to try again. Its easy if your starting plates are cleaner. What are your SAB techniques whats your methods and we can help find your issue and improve your techniques.


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OfflineTryna
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Re: What do you think of my first agar? [Re: mrmazdarx9]
    #24268658 - 04/24/17 03:05 PM (6 years, 10 months ago)

Mad Season, relax...you read my statement wrong..i could've used better punctuation but still

I have only worked in a SAB twice before this attempt, one was approx 18mos ago. Years ago, you could post bad work on Shroomery without getting dogged by elitists.

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InvisibleMad Season
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Re: What do you think of my first agar? [Re: Tryna]
    #24268668 - 04/24/17 03:10 PM (6 years, 10 months ago)

Pardon me? Dogged by elitists? Sorry for telling you to work in your Sab techniques instead of doing open air.. Didn't think it could have been anymore obvious than that. I mean if open air inoc was all good, why would any of us have even made a Sab in the first place? Agar in open air is like begging for some bacteria.. And you literally said..

Quote:

Tryna said:
:frown:  my technique was pretty bad in the SAB. Never had a prob with contaminated doing open air nocs




Idk how I could read that wrong lol


--------------------
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How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
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Re: What do you think of my first agar? [Re: Mad Season]
    #24268960 - 04/24/17 05:33 PM (6 years, 10 months ago)

SAB

https://www.shroomery.org/forums/showflat.php/Number/23990888

Try this. I have also tried this and it works perfectly.

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OfflineTryna
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Re: What do you think of my first agar? [Re: Mad Season]
    #24269961 - 04/25/17 04:21 AM (6 years, 10 months ago)

Quote:

Mad Season said:
Pardon me? Dogged by elitists? Sorry for telling you to work in your Sab techniques instead of doing open air.. Didn't think it could have been anymore obvious than that. I mean if open air inoc was all good, why would any of us have even made a Sab in the first place? Agar in open air is like begging for some bacteria.. And you literally said..

Quote:

Tryna said:
:frown:  my technique was pretty bad in the SAB. Never had a prob with contaminated doing open air nocs




Idk how I could read that wrong lol





This is an irrelevant conversation to have. I never had a problem with contams doing open air nocs. Both times I have used a SAB, i have contam'd...

You are taking a shot, it's ok...a lot of people talk shit over the internet but keep the head and eyes down in the street.

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Offlinemrmazdarx9
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Re: What do you think of my first agar? [Re: Tryna]
    #24269980 - 04/25/17 04:54 AM (6 years, 10 months ago)

:laugh2: now i get the ratings


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Offlinemushpunx
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Re: What do you think of my first agar? [Re: Tryna]
    #24269994 - 04/25/17 05:10 AM (6 years, 10 months ago)

Quote:

Tryna said:
Quote:

Mad Season said:
Pardon me? Dogged by elitists? Sorry for telling you to work in your Sab techniques instead of doing open air.. Didn't think it could have been anymore obvious than that. I mean if open air inoc was all good, why would any of us have even made a Sab in the first place? Agar in open air is like begging for some bacteria.. And you literally said..

Quote:

Tryna said:
:frown:  my technique was pretty bad in the SAB. Never had a prob with contaminated doing open air nocs




Idk how I could read that wrong lol





This is an irrelevant conversation to have. I never had a problem with contams doing open air nocs. Both times I have used a SAB, i have contam'd...

You are taking a shot, it's ok...a lot of people talk shit over the internet but keep the head and eyes down in the street.





It is far from irrelevant, he is trying to help you. If you haven't had contamination working in open air, it is from dumb luck, and that doesn't last.
And that goes triple for working with agar.

To answer your question, all your plates are fucked and you are going to have to start over. If you don't learn to use your SAB properly, this is likely the best you'll get.
This isn't shit talking


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InvisibleMad Season
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Re: What do you think of my first agar? [Re: mushpunx]
    #24270226 - 04/25/17 08:56 AM (6 years, 10 months ago)

:whathesaid:

Yeah I just tell you to change your techniques AFTER you show pics of at least 2 heavily bacterial plates, and OP just calls it talking shit. Funny how ignorance works..

:rofl:

You only tried SAB work twice, both times contaminated, so you blamed the SAB? Sounds an awful lot like shitty techniques to me.. SABs are meant to stop contamination, but are only as good as the growers techniques. That's why instead of giving up after only a meager 2 times, you practice until you have it perfect. My first SAB attempts weren't all that great, in fact most people here were like that. They didn't give up though, and they got to the point of telling you exactly how agar should look.. You can do it too.


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Edited by Mad Season (04/25/17 10:17 AM)

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InvisibleBoogieman47
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Re: What do you think of my first agar? [Re: Mad Season]
    #24270427 - 04/25/17 10:54 AM (6 years, 10 months ago)

Ya op they are right mad season is a good dude ive never seen him lead people the wrong way nor be disrespectful even if the person deserves it .... i get contams sometimes i  front of a flowhood there is very little chanve ypu will get contam free agar doing open air .... spray your sab with soapy water and iso your tools and hands it takes practice in an sab but you will never get clean myc how youre doing it these were all in front of a flow hood i got aspergillus niger mold, bacteria and a clean plate of myc


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InvisibleMad Season
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Re: What do you think of my first agar? [Re: Tryna]
    #24270610 - 04/25/17 12:31 PM (6 years, 10 months ago)

also :whathesaid:

Quote:

Tryna said:
Years ago, you could post bad work on Shroomery without getting dogged by elitists.



Years ago people got shit advice and their grows suffered because of it. My advice is geared to the people who want to get the most they can for the least amount of work, and there's been countless people over the years who have good grows to show from the advice I've given. I'm sorry it isn't filled with unicorns and daisies..

https://www.shroomery.org/forums/showflat.php/Number/21889950

Hopefully that helps. Also check out bodhisatta's links.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.

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