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OfflineOrganic_Magic
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Re: Pastywhyte's Easy Agar Tek - next step? [Re: Zachorion] * 1
    #24239658 - 04/13/17 08:24 AM (6 years, 10 months ago)

Instructions not clear. Dick stuck in the ceiling fan.


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:pipesmoke:Its all fun and games until mushrooms make you gay:pipesmoke:




            click and learn.

Bod's simplified cultivation methods
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Offlineshumer44
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Re: Pasty Agar Tek [Re: shumer44]
    #24241274 - 04/13/17 06:50 PM (6 years, 10 months ago)

Quote:

shumer44 said:
It has been 11 days since I inoculated the agar using this tek, I used glad mini rounds (8 pcs). Only around 10%-20% agar on every mini round is colonized.

Is this normal or this is taking too long?




Bump


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Offlinetryptkaloids
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I'm a teapot


Registered: 02/08/15
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Re: Pasty Agar Tek [Re: shumer44]
    #24241345 - 04/13/17 07:12 PM (6 years, 10 months ago)

you're too concerned. stop worrying, some genes grow slow some grow fast, recipe has a little to do with it too. you should do some reading. USE THE SEARCH ENGINE


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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Offlineshumer44
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Re: Pasty Agar Tek [Re: tryptkaloids]
    #24241349 - 04/13/17 07:14 PM (6 years, 10 months ago)

Quote:

tryptkaloids said:
you're too concerned. stop worrying, some genes grow slow some grow fast, recipe has a little to do with it too. you should do some reading. USE THE SEARCH ENGINE




OKAY


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OfflineD.little91
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Re: Pasty Agar Tek [Re: shumer44]
    #24241530 - 04/13/17 08:20 PM (6 years, 10 months ago)

So I threw out 3 out of 4 plates that were contaminated but on the 4th I cut out the little spots of mold and it looks like things are going strong.




Is this ready to transfer to a grain jar?


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I am a rat who lives in a tree stump in the woods. I have my friends over for tea. We sing songs, drink, and be merry.


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InvisibleTheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
Re: Pasty Agar Tek [Re: D.little91] * 1
    #24241598 - 04/13/17 08:40 PM (6 years, 10 months ago)

Not if you removed mold from it. You never cut contamination away from the culture. You cut part of the culture and transfer that away from the contamination.


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OfflineD.little91
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Re: Pasty Agar Tek [Re: TheMadHatter420]
    #24241632 - 04/13/17 08:51 PM (6 years, 10 months ago)

Quote:

TheMadHatter420 said:
Not if you removed mold from it. You never cut contamination away from the culture. You cut part of the culture and transfer that away from the contamination.




Even if it looks healthy? What I cut out may not have even been mold it may have just been some bits of herbs that were in the potato mix. I made a handful of mistakes because I didn't take it slowly making the plates. I'm going to make more plates maybe this weekend.

Thanks for the info though I will keep that in mind for contaminants in the future.


--------------------
I am a rat who lives in a tree stump in the woods. I have my friends over for tea. We sing songs, drink, and be merry.


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Offlinetryptkaloids
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Re: Pasty Agar Tek [Re: D.little91] * 1
    #24241680 - 04/13/17 09:03 PM (6 years, 10 months ago)

that looks like mold... you don't want mold you want mycelium. the how to use agar link in my sig may help you. in fact, all the links in my sig may help you both


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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InvisibleTheMadHatter420
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Registered: 10/12/16
Posts: 12,941
Re: Pasty Agar Tek [Re: tryptkaloids]
    #24243557 - 04/14/17 01:09 PM (6 years, 10 months ago)

Hey so I whipped up like 19 mini round PP last night. Well a couple fell on side and some at bad angles for the way the agar set. They have not been opened. Can I just take the foil off, microwave and let set flat so the agar will melt and reform on the bottom?


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Offlineblackout
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Re: Pasty Agar Tek [Re: D.little91]
    #24244515 - 04/14/17 07:34 PM (6 years, 10 months ago)

Quote:

D.little91 said:
What I cut out may not have even been mold it may have just been some bits of herbs that were in the potato mix



If you touch contams at all on agar it can spread spores like crazy, or I guess might just disturb & spread very lightweight growth, so you should avoid them like the plague, handle petris with decent good looking growth but with contams with kid gloves. I usually have agar in jars, if contam'd I crack the lid and inject water and boil them, I have opened some in the past to scoop out the agar but saw clouds of horrible green spores rise up the moment I touched it. These were easily visible, if it had just started to grow it probably would have spread like crazy even it not obviously visible. I also just submerge grain jars in water and open the jar so water flows in, and no spores can get in the air.

About 2 weeks ago I had a jar of heavily contam'd agar and microwaved it for 1-2mins, which liquified most it -not all, some was visibly boiling, which shows the issue of hot/cold spots in microwaves. I screwed the lid on and swirled in the bits still solid mixed in and did not reheat. 1-3weeks on it is no showing any growth. I am by no means suggesting 1-2mins is good enough to sterilize! just saying that if you have contam'd jars you want to reuse then another short "heat treatment" might be worthwhile.


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Offlineshumer44
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Re: Pasty Agar Tek [Re: blackout]
    #24247154 - 04/15/17 06:47 PM (6 years, 10 months ago)

Does the thickness of the agar in the glad mini rounds determine the days on how long it will take to colonize the agar?


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OfflineRoyalmensch
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Registered: 03/16/17
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Re: Pasty Agar Tek [Re: shumer44]
    #24247382 - 04/15/17 08:14 PM (6 years, 10 months ago)

I need to pick up some mini rounds, ASAP!


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Offlineshumer44
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Re: Pasty Agar Tek [Re: Royalmensch]
    #24247468 - 04/15/17 08:50 PM (6 years, 10 months ago)

This is my 14 day old agar. Only the top part is colonized, not the middle or bottom

At the bottom, there is some white stuff.



Anything wrong with this?


Edited by shumer44 (04/15/17 08:51 PM)


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Offlinetryptkaloids
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Re: Pasty Agar Tek [Re: shumer44]
    #24248590 - 04/16/17 09:32 AM (6 years, 10 months ago)

I don't know why but I can't zoom in on that. you aren't supposed to have growth anywhere but the top surface. the idea is to keep contams from hiding. transfer the leading clean edge to a new plate


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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Offlineshumer44
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Re: Pasty Agar Tek [Re: tryptkaloids]
    #24249157 - 04/16/17 02:13 PM (6 years, 10 months ago)

I actually made 8 plates with one drop from spore syringe in each. Some are half colonized (top part), some are full colonized (top part).

I thought the mycelium will colonize all of the agar, not just the the top part.

So how do these look?









Edited by shumer44 (04/16/17 02:23 PM)


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InvisibleJosex
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Re: Pasty Agar Tek [Re: shumer44] * 1
    #24249321 - 04/16/17 03:31 PM (6 years, 10 months ago)

You've gotten very quick germination and fast growth so I don't get why you're worried.

Those plates are way overdue for a transfer, you gotta do something quick and quit being a puss :thumbup:

Next time do at least half the thickness of the agar, it'll be easier to take wedges to transfer.

Also research more man, people here gladly answer any questions from people who do their homework, spoon-feeding gets old pretty quick.


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Offlineshumer44
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Re: Pasty Agar Tek [Re: Josex]
    #24249542 - 04/16/17 05:15 PM (6 years, 10 months ago)

Quote:

Josex said:
You've gotten very quick germination and fast growth so I don't get why you're worried.

Those plates are way overdue for a transfer, you gotta do something quick and quit being a puss :thumbup:

Next time do at least half the thickness of the agar, it'll be easier to take wedges to transfer.

Also research more man, people here gladly answer any questions from people who do their homework, spoon-feeding gets old pretty quick.




Thank You.

Coz I watched this video and noticed that his agar was so white, not same as mine and i concluded that I have to wait more.




Edited by shumer44 (04/16/17 05:41 PM)


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Offlinetryptkaloids
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Re: Pasty Agar Tek [Re: shumer44]
    #24250476 - 04/16/17 11:21 PM (6 years, 10 months ago)

you want to transfer to a new set of plates to make sure it is clean first.
check out how it should and shouldn't look did you read the links in my sig I suggested


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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InvisibleJosex
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Re: Pasty Agar Tek [Re: shumer44]
    #24255080 - 04/18/17 06:33 PM (6 years, 10 months ago)

Quote:

shumer44 said:
Quote:

Josex said:
You've gotten very quick germination and fast growth so I don't get why you're worried.

Those plates are way overdue for a transfer, you gotta do something quick and quit being a puss :thumbup:

Next time do at least half the thickness of the agar, it'll be easier to take wedges to transfer.

Also research more man, people here gladly answer any questions from people who do their homework, spoon-feeding gets old pretty quick.




Thank You.

Coz I watched this video and noticed that his agar was so white, not same as mine and i concluded that I have to wait more.






I've been subbed to that guy's channel for a while, dude's got mad skillz.

You can see his plates are 100% colonized because he's inoculating grains, you let plates colonize all the way (or nearly) when you are going to use them to inoculate grains.

Look, I found a quote I posted some time ago in response to someone who was asking lots of questions about agar, he loved to be spoon-fed, so I gave him the "ultimate" list of links on agar, all of them have the "Josex seal of approval 2016" so it's good stuff :thumbup:

All you need to know (and more) is there, seriously, look them up.

Quote:

Josex said:
There you go, with these links you can finally spoon-feed yourself, you ain't gonna need nobody no more  :mypleasure:

Why you should start with agar (take a look at the links in the OP as well)

Pasty's plates

Videos on how to work with Pasty's plates and sterile technique

The Tiger Drop - Grow From Your Own Prints With No Contamination - Tek for n00bs

How to make a soft agar mix to germinate spores and to make a blenderless LI

Blenderless LI

Agar Portal

Improve your sterile technique! (a comprehensive guide to agar)

And also very important, watch all of RogerRabbit's videos on agar and sterile technique, they're badass (included in the link "Agar Portal").

Bonus
SAB




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OfflineZachorion
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Re: Pasty Agar Tek [Re: Josex]
    #24263873 - 04/22/17 11:46 AM (6 years, 9 months ago)

I've made three batches of pasty plates and am using them in various ways.  I wondered if I should let a plate colonize to the same degree for all uses, ie., tiger drop, LI, etc..

I though I read somewhere that you let the plate grow out more for a certain thing but of course, I can't remember where I read it.

tia


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