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Amazon Shop: ½ Pint Jars, Agar, Rye Grain

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OfflineRushMore
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Grain on "Hold" a Sterile Window or Flush It ???
    #24261073 - 04/21/17 05:55 AM (1 month, 2 days ago)

Had to get surgery instead of wrapping up a full PC of Rye Quarts. Haven't
Opened the lid or disturbed the Shaker (closed-loop still).
It's been 72-hrs on my stove.
Toss the lot or throw agar wedges in and go???


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InvisibleHamington Loafburg
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Re: Grain on "Hold" a Sterile Window or Flush It ??? [Re: RushMore]
    #24261085 - 04/21/17 06:26 AM (1 month, 2 days ago)

If the jars have gone through a full sterilization cycle, then inoculating them after a 72 hour period would be ok.  It's not the best practice, but if grains are sterilized properly, then a few days will be fine. 

If the jars went through a partial sterilization cycle, or even worse, the jars have been sitting in the PC without having been through a sterilization cycle at all, retrieve the jars from the PC, open one of the lids, and give the grains a sniff. 

If the grains don't smell like anything other than fresh grains toss the lot, and begin again.  If they still smell fresh, put them through a full sterilization cycle, and sterilize them for an extra half an hour.


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Re: Grain on "Hold" a Sterile Window or Flush It ??? [Re: Hamington Loafburg]
    #24261150 - 04/21/17 08:08 AM (1 month, 2 days ago)

My PC cycle was completely RR-Style at 120min 15psi. Just to be sure, I was thinking about just warming up to 15psi again for 1-hr to cover any fresh mis-steps developing within.
Will the Starches/Rye saccaharides be over processed ya think? Maybe 30-min instead?
I'm leary to do a smell test since it's gotta be re-run anyway...
Hamloaf you aren't saying run it up for 2 and a 1/2 hours are you?
That would prolly denature any proteins into mush like ryeberry 'oatmeal' wouldn't it?


Edited by RushMore (04/21/17 08:14 AM)


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InvisibleHamington Loafburg
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Re: Grain on "Hold" a Sterile Window or Flush It ??? [Re: RushMore]
    #24261171 - 04/21/17 08:25 AM (1 month, 2 days ago)

Nah, RR sterilizes his jars for 90 minutes.  Not 120, so a two hour sterilization cycle was being suggested.  You're right about the amount of psi's he uses though.

Resterilizing for an hour won't hurt anything.


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Re: Grain on "Hold" a Sterile Window or Flush It ??? [Re: Hamington Loafburg]
    #24261406 - 04/21/17 11:21 AM (1 month, 2 days ago)

Thanks. Wish me luck Hamloaf. I'll post pics when results are in.


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Amazon Shop: ½ Pint Jars, Agar, Rye Grain

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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