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OfflineGrambo
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Registered: 04/04/16
Posts: 212
Loc: Canaba Flag
Last seen: 3 years, 10 months
Re: frustrated [Re: Grambo]
    #24256131 - 04/19/17 03:44 AM (7 years, 8 months ago)

The myc certainatly did become very aggressive and stood a few millimetres off the agar, so could be, but the blobs were never there in the beginning.  I believe it must be airborne as the last two transfers rendered me only one purely clean culture out of 2 to 3 cultures taken. If the  bacteria is piggybacking, then it would be on each and every transfer I do.

The transfers that end up contaminating do so within two days, the clean cultures that don't contaminate last for a week or even more with no signs of bacteria.

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InvisibleReblackah
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Registered: 01/07/15
Posts: 13,770
Re: frustrated *DELETED* [Re: Grambo]
    #24256146 - 04/19/17 03:53 AM (7 years, 8 months ago)

Post deleted by LizardWizard

Reason for deletion: ok

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OfflineGrambo
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Registered: 04/04/16
Posts: 212
Loc: Canaba Flag
Last seen: 3 years, 10 months
Re: frustrated [Re: Reblackah]
    #24258611 - 04/19/17 11:36 PM (7 years, 8 months ago)

Transfers always on the leading edge.

The myc I have always let grow to the size of a half dollar or loonie, a few times the size of a poker chip but there were no signs of contam or so it seemed.  Maybe that is my vector, perhaps I should be doing my transfers sooner?

Really appreciate the help guys.

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InvisibleMad Season
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Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: frustrated [Re: Grambo]
    #24258681 - 04/20/17 12:30 AM (7 years, 8 months ago)

Nah you can transfer at any point and it takes 20+ plates all hitting the edge before you'll experience any form of senescence.


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