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Offlinekrypton4097
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Registered: 03/12/17
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Last seen: 1 year, 3 months
Little help here.....first time agar with PE...whats my next step?
    #24235696 - 04/11/17 07:09 PM (7 years, 9 months ago)

So I got ahold of a special little PE strain. I did my first agar to agar transfer and this is about 2 weeks out......I am new at this but I know that I have very little rhizomorphic formation.....mostly tomentose. I don't see any contamination in the glasses, I realize its hard to see due to the glass selection. My question is should I try and repeat the agar process to look for better growth or is this something I could try to spawn to grain?








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OfflineAztecDeathWhistle
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Registered: 12/11/16
Posts: 186
Last seen: 4 years, 3 months
Re: Little help here.....first time agar with PE...whats my next step? [Re: krypton4097]
    #24235720 - 04/11/17 07:17 PM (7 years, 9 months ago)

Copy it to multiple plates.
Wait for those to fill out.
Use them with Pasty's Tic Tac Toe tek to spawn them to grain.

Or spawn multiple jars with it, it'll just take them longer to colonize.

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Offlinekrypton4097
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Registered: 03/12/17
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Re: Little help here.....first time agar with PE...whats my next step? [Re: AztecDeathWhistle]
    #24235727 - 04/11/17 07:20 PM (7 years, 9 months ago)

Hey Aztec, sorry I didnt write this in the post but this is actually 7 different agar jars, should I continue to let grow and then try to spawn to grain?

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OfflineKKVguitarist
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Re: Little help here.....first time agar with PE...whats my next step? [Re: AztecDeathWhistle]
    #24235733 - 04/11/17 07:22 PM (7 years, 9 months ago)

Yeah grow a section out on another plate, tic tac toe it, then g2g or LI and spread that bad boy around.

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OfflineOrganic_Magic
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Re: Little help here.....first time agar with PE...whats my next step? [Re: krypton4097]
    #24235735 - 04/11/17 07:24 PM (7 years, 9 months ago)

Nothing wrong with tomentose growth. You should look at growth speed more than growth type. If you are sure they are clean you could go ahead and do some A2G. For future reference you can get away with using less agar in your jars and it actually makes it easier to do transfers.

looks good. :pipesmoke:


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OfflineAztecDeathWhistle
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Registered: 12/11/16
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Re: Little help here.....first time agar with PE...whats my next step? [Re: krypton4097]
    #24235756 - 04/11/17 07:36 PM (7 years, 9 months ago)

Quote:

krypton4097 said:
Hey Aztec, sorry I didnt write this in the post but this is actually 7 different agar jars, should I continue to let grow and then try to spawn to grain?




If they all look like that you are fine.

Personally I would put one in the fridge. Then tic tac toe the other 6 to 6 jars.

As KKV said you could do grain to grain or Liquid Inoculation from there.

IMHO, LI introduces far too many extra steps (opportunities to fuck up) for what you get out of it.

If you already have six plates I wouldn't bother with G2G either.

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OfflineMycologist217
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Re: Little help here.....first time agar with PE...whats my next step? [Re: AztecDeathWhistle]
    #24255115 - 04/18/17 06:49 PM (7 years, 8 months ago)

my man!!! repping the m217-a! Show us the grain you inoculated!


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Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.

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Offlinemushpunx
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Re: Little help here.....first time agar with PE...whats my next step? [Re: Mycologist217]
    #24255421 - 04/18/17 08:50 PM (7 years, 8 months ago)

As long as it looks clean go ahead and inoculate your spawn.

If you are only running a couple tubs you can just go all agar to grain, I usually split 1 plate between 4-5 jars so 1 plate per tub.

Or you can inoculate grain masters, pick your best plate and inoculate however many masters you need for G2G


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OfflineOrganic_Magic
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Registered: 10/26/14
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Re: Little help here.....first time agar with PE...whats my next step? [Re: mushpunx]
    #24255499 - 04/18/17 09:22 PM (7 years, 8 months ago)

:whathesaid:

I usually just do whole plates that are definitely clean to a few masters and then expand from there. Faster that way IMO.


--------------------
:pipesmoke:Its all fun and games until mushrooms make you gay:pipesmoke:




            click and learn.

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