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Offlineichugwindex
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Re: needing experienced eyes for oats [Re: bodhisatta]
    #24253949 - 04/18/17 09:31 AM (7 years, 8 months ago)

Thanks bod:thumbsup: you always give me good direction. I did shake at about 35 percent colonized and it seemed to recover fine.


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Only hope can give rise to the emotion we call despair. But it is nearly impossible for a man to try to live without hope, so I guess that leaves Man no choice but to walk around with despair as his companion.

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InvisiblemushboyMDiscord
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Re: needing experienced eyes for oats [Re: mushboy]
    #24253951 - 04/18/17 09:32 AM (7 years, 8 months ago)

but you break up spawn anyway.

Quote:

mushboy said:
The fact that it didn't work for one person, once is no reason to advise against tried, and true methods





no doubt! and ill :takingnotes: because i dig it but at the same time its just breaking up myc. its not like im scattering into a field. and since it gets broken up anyway to be spawned, it gets diminished that way, right?

so to not diminish it at all why not just top fruit everything?

Quote:

wouldn't be a suggested limit on how many times we expanded our cultures before spawning to a bulk substrate before senescence is noticed.




thats why we stop expanding? :confused:

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OfflinehamloafM
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Re: needing experienced eyes for oats [Re: mushboy]
    #24253959 - 04/18/17 09:36 AM (7 years, 8 months ago)

Correct, because there is only so many times that the mycelium can be broken up, and recover before it just won't anymore.


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InvisiblebodhisattaMDiscordReddit
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Re: needing experienced eyes for oats [Re: ichugwindex] * 1
    #24253960 - 04/18/17 09:36 AM (7 years, 8 months ago)

Senescence is far more related to age of a culture out of cold storage than expansion.

You can expand 1:10000000 through series of 1:10 transfers if you wanted. with no senescence issues if you did it fast enough

The old rule of thumb 3 g2g generations was because of the speed of most cultivators and to keep the mycelium vigerous so it wasn't tired of growing on spawn and the same kind of spawn. Which is different than senescence which is actual genetic degradation.

Quote:

RogerRabbit said:
Another misunderstood aspect is chronological time, not just the amount of cell divisions.  A single kernel of colonized grain can be expanded to fill a large truck of grains without senescence if one works fast.  However,if you took each jar of grains and let it sit for a month before making the transfer, you wouldn't get nearly as far, even if they were refrigerated.  This is why we keep the masters on test tubes and don't begin the expansion until the time comes.
RR





The senescence worry is SO overplayed by the community.

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InvisiblemushboyMDiscord
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Re: needing experienced eyes for oats [Re: bodhisatta]
    #24253965 - 04/18/17 09:39 AM (7 years, 8 months ago)

the only real valid point ive ever heard is it slows things down. well..

im not a rocket ship on count down. i dont care how long it takes. i got shrooms for life, whats another few days
added to the process? now if peoples drive from the 'hobbyist' point of view is, bigger faster better then more power to you.

but to say its detrimental is kinda being obtuse. or im being an asshole. either way :shrug:

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