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Offlinetombosley8
Full on... Bossley Baggins
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Registered: 10/14/13
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Re: Lucy (Leucistic) Golden Teacher in a 50 Quart Monotub. [Re: TheMadHatter420] * 1
    #24428149 - 06/23/17 12:56 AM (7 years, 6 months ago)

oh yeah I also nocced cakes with LI the other day and they are almost colonized


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Edited by tombosley8 (06/23/17 12:58 AM)

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InvisibleTheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,943
Re: Lucy (Leucistic) Golden Teacher in a 50 Quart Monotub. [Re: tombosley8]
    #24428156 - 06/23/17 01:00 AM (7 years, 6 months ago)

I have been considering knocking up some cakes with Lucy.


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Invisiblejkz
Padawan
I'm a teapot User Gallery


Registered: 06/16/13
Posts: 1,910
Loc: Coruscant
Re: Lucy (Leucistic) Golden Teacher in a 50 Quart Monotub. [Re: TheMadHatter420]
    #24428158 - 06/23/17 01:01 AM (7 years, 6 months ago)

If the LC I made the other day is clean enough, I plan to test it on some cakes haha


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OfflineKenetic
Nam Sayin
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Re: Lucy (Leucistic) Golden Teacher in a 50 Quart Monotub. [Re: jkz]
    #24428334 - 06/23/17 04:53 AM (7 years, 6 months ago)

Just out if curiosity Ham, what method do you use to collect spores?

I always put a little pin in the top of the cap, hold with one hand and decapitate with a scalpel in the other hand.  Place through arm holes on a big sheet of clean foil that is already placed in my sab (I do multiples at a time).  Let sit for a day, iso my gloved hands and arms and remove caps.  Let sit for another day to dry. 

Then i cut into squares and fold.  I'm sure there is a better way, i particularly dont like cutting the foil afterwards.  Hmmmm der maybe I should precut the squares....


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Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet

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Invisibleeatyualive
Eat's You Alive :)
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Registered: 08/17/01
Posts: 19,026
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Re: Lucy (Leucistic) Golden Teacher in a 50 Quart Monotub. [Re: Kenetic]
    #24428428 - 06/23/17 06:36 AM (7 years, 6 months ago)

I cut the foil first into 8x8 inch squares. Then when print is complete I fold it over in half. And fold on 3 sides. When you open the print it has a nice cover over it for protection to swipe spores ECT. I've got a diagram in my print method thread that shows this. I'll pm it to you later. Or dig in my references.


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OfflineKenetic
Nam Sayin
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Re: Lucy (Leucistic) Golden Teacher in a 50 Quart Monotub. [Re: eatyualive]
    #24428433 - 06/23/17 06:38 AM (7 years, 6 months ago)

Nice.  I'll look it up in your thread references.  I'm working on improving my giveaways.

(And my cultivation in general.  I need to treat this hobby like the art that it is.)


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Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet

Edited by Kenetic (06/23/17 09:37 AM)

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InvisibleTheMadHatter420
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Registered: 10/12/16
Posts: 12,943
Re: Lucy (Leucistic) Golden Teacher in a 50 Quart Monotub. [Re: Kenetic]
    #24428765 - 06/23/17 10:04 AM (7 years, 6 months ago)

I used to cut 4x8 pieces, put cap to one half, and set a clean pint jar over it, while in SAB. Then let set 24 hours. Next day I removed cap and would stick a piece of wire under the very edge of the jar to allow air exchange. Easy as fuck to sterilize some thin jewelry wire with a lighter. After another 24 hours they would be dry. Fold foil in half and fold 3 sides.

Now I have been making sheets of prints in a Rubbermaid container in my SAB. The cutting apart of the prints does suck, but I soak my scissors in alcohol and them take the torch to the blades. I then tare a strip of foil about 8 inch by length of the box. Mist with alcohol and fold in half. I then set it in the sab and fold open some and wait for it to dry. Then cut the folded piece wide enough for the print and bent shut foil around edges. Slip print in and fold 3 sides. You do have to make DAMN SURE the foil is dry after alcohol spray. I get 4-5 prints from each bent over piece. You could just print on this, but I REALLY like having the print not on the "cover" foil, but that's a me thing.


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InvisibleMorelman
LC Master
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Registered: 05/17/06
Posts: 2,625
Loc: Hiding under an Elm Tree Flag
Re: Lucy (Leucistic) Golden Teacher in a 50 Quart Monotub. [Re: TheMadHatter420] * 1
    #24429180 - 06/23/17 12:43 PM (7 years, 6 months ago)



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InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
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Registered: 04/30/13
Posts: 61,915
Loc: Milky way
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Re: Lucy (Leucistic) Golden Teacher in a 50 Quart Monotub. [Re: Morelman] * 1
    #24429199 - 06/23/17 12:51 PM (7 years, 6 months ago)

can you autoclave that hole punch of just sanitize it?
I'd want to keep the foil off the roll sterile
you could do a stack of a few foils and throw out the top and bottom I suppose

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InvisibleMorelman
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Re: Lucy (Leucistic) Golden Teacher in a 50 Quart Monotub. [Re: bodhisatta] * 1
    #24429203 - 06/23/17 12:53 PM (7 years, 6 months ago)

I pc the foil discs in a wide mouth jar.


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InvisiblebodhisattaMDiscordReddit
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Re: Lucy (Leucistic) Golden Teacher in a 50 Quart Monotub. [Re: Morelman] * 1
    #24429206 - 06/23/17 12:54 PM (7 years, 6 months ago)

:thumbup:

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OfflinehamloafM
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Re: Lucy (Leucistic) Golden Teacher in a 50 Quart Monotub. [Re: bodhisatta] * 1
    #24429222 - 06/23/17 01:02 PM (7 years, 6 months ago)

Nice flow of conversation.  This pleases me.  Thank you.


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How I Get Stuff done. - My Reference Guide. - My Grows.

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InvisiblemushboyMDiscord
modboy
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Re: Lucy (Leucistic) Golden Teacher in a 50 Quart Monotub. [Re: hamloaf] * 1
    #24429236 - 06/23/17 01:08 PM (7 years, 6 months ago)

this germ plate pleases me. keeps pushing out pins. ive cloned 3 so far. why not 4?


this one is ready for its first cuts. i got lots of good places. i could wait but the dish is sloppy.


and this guy too. ill be taking cuts from 2-5 o clock.


i nver thought to pc foil like that. not a bad idea. i always wipe off with iso but for giveaways ill be doing that.:thumbup:

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OfflinehamloafM
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Re: Lucy (Leucistic) Golden Teacher in a 50 Quart Monotub. [Re: mushboy]
    #24429278 - 06/23/17 01:30 PM (7 years, 6 months ago)

Nice work, mushboy.  You look like you are having fun.  :thumbup:

In other relating leucestic golden teacher news; I made a 5 part + 2 part cake mix the other day.  Had some grains, and cake mix left over, so I threw the grains inside of a spawn bag with the cake mix on top of it, and sterilized the bag for 3 hours at 20 psi's (All American), let it cool, and inoculated it with that LGT LC by free pouring half the contents of the jar (LC) into the bag.  No shake, nothing special.  Sealed up the bag as usual, and placed it on the shelf to colonize/incubate.

   


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How I Get Stuff done. - My Reference Guide. - My Grows.

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InvisibleGerms
Space Force
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Registered: 06/26/11
Posts: 4,607
Loc: Texas
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Re: Lucy (Leucistic) Golden Teacher in a 50 Quart Monotub. [Re: hamloaf]
    #24429424 - 06/23/17 02:36 PM (7 years, 6 months ago)


First tub is looking kinda sad. That's what bacterial spawn will get ya


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OfflineKenetic
Nam Sayin
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Re: Lucy (Leucistic) Golden Teacher in a 50 Quart Monotub. [Re: Germs]
    #24429490 - 06/23/17 03:03 PM (7 years, 6 months ago)

I'm pretty sure I'll experience something similar on some of my mini's.  My wheat spawn had some metabs during the end of colonization and the myc looked stressed.  Started out good though but.... 
I quit using rye and wheat because of this problem, either I'm prepping it incorrectly or pcing incorrectly, who knows.  I've heard grain farmers treat rye and similar food grade grains with endospores to limit the liability of the grains growing a harmful mold if stored or prepped incorrectly by consumers, and maybe my pc can't kill these endospores. 
Some say a pc will kill anything, some (like me) think it actually doesn't.  I can never see or smell colonies of bacteria but I see stalled growth and metabs, so I know it's there.

I moved on to pure whole white millet a while ago (birdfood, not a wbs mix) and I have never had a bacterial jar with that grain. 

I'm willing to accept that I'm doing something wrong but I can't pinpoint what it could be.  I have definitely seen a pattern with rye and similar food grade grains (sourced from a health food store).
My rye prep is on point, I vent the pc for 10 minutes, and pc for 2 hours after the pc is stabilized.  I'll just stick with millet.


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Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet

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InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
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Re: Lucy (Leucistic) Golden Teacher in a 50 Quart Monotub. [Re: Kenetic]
    #24429524 - 06/23/17 03:21 PM (7 years, 6 months ago)

they make strips with 1,000,000 bacterial endospores from much harder to kill species than ones found in grain and a regular old 15m at 15psi kills all of them. we just use longer times because we're sterilizing grains not liquids or instruments.

we don't run into
Geobacillus stearothermophilus
Bacillus atrophaeus
etc..
we're getting clostridium for the most part.

they also make vials with 107 spores per mL so you can use 0.1mL and add it to agar then sterilize the agar and incubate dishes made with it to validate your autoclave and make sure that 15m at 15psi is working to sterilize 1L or smaller volumes of agar.

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InvisibleTheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,943
Re: Lucy (Leucistic) Golden Teacher in a 50 Quart Monotub. [Re: bodhisatta]
    #24429641 - 06/23/17 04:16 PM (7 years, 6 months ago)

That's cool as fuck info Bod.


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Invisiblemynakedrat
The phantom hourglass
I'm a teapot User Gallery


Registered: 02/16/17
Posts: 4,629
Loc: Inner Astral levels Flag
Re: Lucy (Leucistic) Golden Teacher in a 50 Quart Monotub. [Re: TheMadHatter420]
    #24429677 - 06/23/17 04:33 PM (7 years, 6 months ago)

Or. You could. Wipe between your toes, then swab a plate, then PC

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OfflineKenetic
Nam Sayin
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Registered: 08/24/14
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Re: Lucy (Leucistic) Golden Teacher in a 50 Quart Monotub. [Re: TheMadHatter420]
    #24429689 - 06/23/17 04:38 PM (7 years, 6 months ago)

I wonder what the deal is? Unless my dishes have some bacteria that I am unable to see I'm at a complete loss.


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Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet

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