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OfflinehamloafM
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Re: Lucy (Leucistic) Golden Teacher in a 50 Quart Monotub. [Re: spacechildo]
    #24329470 - 05/18/17 09:33 AM (7 years, 7 months ago)

You are correct.
These prints were taken 4/20/2017.


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InvisiblebodhisattaMDiscordReddit
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Re: Lucy (Leucistic) Golden Teacher in a 50 Quart Monotub. [Re: hamloaf]
    #24329471 - 05/18/17 09:34 AM (7 years, 7 months ago)

I showed Alan some micrographs of spores i plated and he mentioned that cube spores can easily germinate in under 48 hours given proper conditions and being fresh.

I had noticed germination in like 40 hours
Hams spores probably took similar times by the time you see growth it's already germinated some time ago

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Offlinespacechildo
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Re: Lucy (Leucistic) Golden Teacher in a 50 Quart Monotub. [Re: hamloaf]
    #24329496 - 05/18/17 09:46 AM (7 years, 7 months ago)

Quote:

Hamington Loafburg said:
You are correct.
These prints were taken 4/20/2017.




right. I'd be surprised if anyone found they took more than a couple days to germinate, really.

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OfflineKenetic
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Re: Lucy (Leucistic) Golden Teacher in a 50 Quart Monotub. [Re: spacechildo] * 1
    #24329592 - 05/18/17 10:23 AM (7 years, 7 months ago)

Well regardless, they are definitely viable.


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Re: Lucy (Leucistic) Golden Teacher in a 50 Quart Monotub. [Re: Kenetic]
    #24329655 - 05/18/17 10:44 AM (7 years, 7 months ago)

I just inoculated a bag of crumbled, spent, sterilized, cube substrate (coir) with 20ml of King Oyster GLI.



If this works, I'll repeat the process with Shaggy Mane. My spent substrate should be soil by the time I dispose of it.

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Re: Lucy (Leucistic) Golden Teacher in a 50 Quart Monotub. [Re: Morelman]
    #24329715 - 05/18/17 11:05 AM (7 years, 7 months ago)

Nice! Are you starting a thread by any chance? Journal where we can follow this? It would be really cool if this worked!

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InvisibleMorelman
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Re: Lucy (Leucistic) Golden Teacher in a 50 Quart Monotub. [Re: Reblackah]
    #24329760 - 05/18/17 11:20 AM (7 years, 7 months ago)

Quote:

GoldenPreacher said:
Nice! Are you starting a thread by any chance? Journal where we can follow this? It would be really cool if this worked!




I will update the project in my journal, here:
https://www.shroomery.org/forums/showflat.php/Number/24320820#24320820

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OfflinehamloafM
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Re: Lucy (Leucistic) Golden Teacher in a 50 Quart Monotub. [Re: Morelman]
    #24329808 - 05/18/17 11:41 AM (7 years, 7 months ago)

That's awesome Morelman. 
Be watching that one for sure.
What's up with Lucy, though?


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Edited by hamloaf (05/18/17 11:47 AM)

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InvisibleMorelman
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Re: Lucy (Leucistic) Golden Teacher in a 50 Quart Monotub. [Re: hamloaf] * 1
    #24329868 - 05/18/17 12:07 PM (7 years, 7 months ago)

Quote:

Hamington Loafburg said:
That's awesome Morelman. 
Be watching that one for sure.
What's up with Lucy, though?





Thanks!

Not much to report on Lucy today. I inoculated a big ugly bag of sterilized rye and coir several days ago. She's taking her time to recover. I think I see some lighter grains behind one of the injection ports.
The two extra GLI syringes are very dense and I'm shaking daily to keep the inoculant from settling until they recover.
I'm going to empty one or both into a pint of sterile water to dilute and make more leaner syringes.

Done...


Edited by Morelman (05/18/17 01:06 PM)

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InvisibleRaven44
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Re: Lucy (Leucistic) Golden Teacher in a 50 Quart Monotub. [Re: Morelman]
    #24332423 - 05/19/17 09:00 AM (7 years, 7 months ago)

Hey ham, I have another quick question for you

When you use shallower substrate depths, do you ever "fruit at spawning"??

Or do you tape holes when using shallower sub depths to prevent excessive loss of moisture? Then un-tape holes when you want to introduce fruiting conditions?

Or do you still introduce to fruiting conditions from day 1, to get a quicker pinset?

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OfflinehamloafM
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Re: Lucy (Leucistic) Golden Teacher in a 50 Quart Monotub. [Re: Raven44]
    #24332729 - 05/19/17 11:27 AM (7 years, 7 months ago)

My climate is pretty humid, so I get away with stuffing the holes of my tub with polyfil very tightly for spawn run. 
Once pins arrive (usually about 8-12 days), I loosen the polyfil about 75%, then manually fan a couple times a day.
I also throw an extra quart of water into my coir prep.


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Re: Lucy (Leucistic) Golden Teacher in a 50 Quart Monotub. *DELETED* [Re: hamloaf]
    #24332741 - 05/19/17 11:34 AM (7 years, 7 months ago)

Post deleted by LizardWizard

Reason for deletion: ok

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OfflinehamloafM
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Re: Lucy (Leucistic) Golden Teacher in a 50 Quart Monotub. [Re: Reblackah]
    #24332747 - 05/19/17 11:36 AM (7 years, 7 months ago)

I prep my coir 6 bricks at a time in a 15 gallon storage tote.
Not one brick at a time in a bucket.
The "excess" water is distributed evenly.
A shallow substrate colonizes faster than a deeper one.

Edit to add.  I also, mix coir at equal parts with hydrated, pasteurized horse manure.


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Re: Lucy (Leucistic) Golden Teacher in a 50 Quart Monotub. *DELETED* [Re: hamloaf]
    #24332775 - 05/19/17 11:50 AM (7 years, 7 months ago)

Post deleted by LizardWizard

Reason for deletion: ok

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InvisiblemushboyMDiscord
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Re: Lucy (Leucistic) Golden Teacher in a 50 Quart Monotub. [Re: Reblackah] * 1
    #24332789 - 05/19/17 11:59 AM (7 years, 7 months ago)


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Re: Lucy (Leucistic) Golden Teacher in a 50 Quart Monotub. [Re: mushboy] * 1
    #24332813 - 05/19/17 12:08 PM (7 years, 7 months ago)

T1 has been made!

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Re: Lucy (Leucistic) Golden Teacher in a 50 Quart Monotub. [Re: Reblackah]
    #24332831 - 05/19/17 12:19 PM (7 years, 7 months ago)

Tight mushboy!
We are about at the same point.



Wedge to grain transfer.
Each jar got nocced with half a plate.
I made 12 sector transfers too.
Going to town on an isolation program with these. 

Quote:

GoldenPreacher said:
Do you not pasteurize the coir?




I hot water bath the coir.
https://www.shroomery.org/forums/showflat.php/Number/22290008#22290008
(Excuse the incompleteness of that link.  Will re-do next week)
The manure gets pasteurized separately.
https://www.shroomery.org/forums/showflat.php/Number/21126535#21126535
The materials are mixed together at equal parts for bulk spawning.


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Re: Lucy (Leucistic) Golden Teacher in a 50 Quart Monotub. [Re: hamloaf]
    #24333493 - 05/19/17 04:37 PM (7 years, 7 months ago)

i am 2 transfers in, with lots of different cultures. i did a serial dilution streak so i had lots of colonies to choose from, many of which were somewhat genetically limited (in terms of the number of spores that could have gone into those areas)

looking good so far, nothing rhizo but fairly aggressive. various colonies on the streaked plate look very different, with distinct zones of inhibition forming between them

will probably use one of the earlier plates to a2g and make an MS bucket while i finish getting a few isolates


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Re: Lucy (Leucistic) Golden Teacher in a 50 Quart Monotub. [Re: c10h12n2o]
    #24333540 - 05/19/17 05:03 PM (7 years, 7 months ago)

My two first transfers are growing well.

Need three or four more and I'll inoculate. Can't wait


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Re: Lucy (Leucistic) Golden Teacher in a 50 Quart Monotub. [Re: Moabfighter]
    #24333770 - 05/19/17 06:24 PM (7 years, 7 months ago)

Quote:

aren't these spores just a few weeks old? spore germination time and spore age goes together, fresh spores should take 2-3 days tops, older ones like 5 years up could take up to 2-3 weeks IME.



ive had 5 plus year old spores take 3-5 days. its pretty amazing what nature can do.:thumbup:


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Shop: PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Mushroom-Hut Liquid Cultures   OlympusMyco.com No Unicorns Here—Just Quality Bags That Work   Original Sensible Seeds Bulk Cannabis Seeds   Myyco.com Golden Teacher Liquid Culture For Sale   North Spore Bulk Substrate   Left Coast Kratom Buy Kratom Extract   MagicBag.co All-In-One Bags That Don't Suck   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Kraken Kratom Red Vein Kratom


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