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OfflineGodseed313
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Re: Lucy (Leucistic) Golden Teacher in a 50 Quart Monotub. [Re: hamloaf]
    #24304133 - 05/08/17 08:31 AM (7 years, 8 months ago)



First attempt at agar and my first little ghetto SAB yay! Made up a syringe and knocked up a few 1/2 pints and 2 plates, fingers crossed and I'll check em tonight after work. Been 48 hours so I'm hoping to see some growth.
And plenty Lucy spores set aside just in case I completely screwed up ha I don't want to let Ham down!

Thanks man, you are a gentleman and a scholar, cheers


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InvisiblebodhisattaMDiscordReddit
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Re: Lucy (Leucistic) Golden Teacher in a 50 Quart Monotub. [Re: hamloaf] * 1
    #24304164 - 05/08/17 08:46 AM (7 years, 8 months ago)

Quote:

Hamington Loafburg said:
Cool, Mad.  On agar mycelium runs faster than bacteria, and mold, so another cool trick is to take your transfers before the culture runs into the walls of the plates, transferring away from the very leading edge of the culture with a wedge of culture cut no larger than a grain of rice.  That way the mycelium has a chance to out run any contaminate that may be present.  Once the culture hits the walls, any contaminate that may be hitching a ride will be able to catch up with the mycelium, and the whole culture becomes infected.



Sorry ham I hate to be hard on you but that's some of the worst advice I've seen come out of your mouth.

Molds almost always run faster than mycelium

Molds produce spores as they go so you're letting it sporulate on the plate and contaminate leading edges of "seemingly" clean mycelium at that point

If getting away from mold and bacteria quick rapid transfers are the objective. Never let a plate run, mycelium won't out run molds

Also with contaminated plates you can't use a flow hood use a still air box.

If you have mold you're getting away from if you let that plate grow longer than a day or two the whole surface will be littered with spores so any transfers are playing with fire

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Invisiblenatedawgnow
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Re: Lucy (Leucistic) Golden Teacher in a 50 Quart Monotub. [Re: bodhisatta]
    #24304167 - 05/08/17 08:48 AM (7 years, 8 months ago)

Pretty sure what he's saying is to transfer before it hits the edge of the plate
i.e. as soon as possible, before it grows out that far.


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InvisiblebodhisattaMDiscordReddit
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Re: Lucy (Leucistic) Golden Teacher in a 50 Quart Monotub. [Re: natedawgnow]
    #24304177 - 05/08/17 08:50 AM (7 years, 8 months ago)

Im concerned about mycelium running faster than mold lol

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Invisiblenatedawgnow
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Re: Lucy (Leucistic) Golden Teacher in a 50 Quart Monotub. [Re: bodhisatta]
    #24304180 - 05/08/17 08:51 AM (7 years, 8 months ago)

Fair enough :lol:


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InvisiblebodhisattaMDiscordReddit
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Re: Lucy (Leucistic) Golden Teacher in a 50 Quart Monotub. [Re: natedawgnow]
    #24304188 - 05/08/17 08:54 AM (7 years, 8 months ago)

One of the easiest ways to identify mold is its speed

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InvisibleTheMadHatter420
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Re: Lucy (Leucistic) Golden Teacher in a 50 Quart Monotub. [Re: natedawgnow]
    #24304189 - 05/08/17 08:55 AM (7 years, 8 months ago)

99% of the time I toss mold plates, I recently, for the 1st time, took a couple transfers from a plate with mold. That was only because it had just showed up and was tiny as fuck. Don't know what its called but I have had it before. Makes the little white circles that quickly goes green in the middle with spores. I only did it because all was white and so small. If it fails, I never do it again. I have been taking my transfers when the mycelium is like 1/2 to 3/4 inch from the center of plate. I want just enough time that it has leapt past bacteria.


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InvisiblebodhisattaMDiscordReddit
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Re: Lucy (Leucistic) Golden Teacher in a 50 Quart Monotub. [Re: TheMadHatter420]
    #24304198 - 05/08/17 08:57 AM (7 years, 8 months ago)


Like that? Probably some kind of aspergillus

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OfflinehamloafM
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Re: Lucy (Leucistic) Golden Teacher in a 50 Quart Monotub. [Re: bodhisatta]
    #24304223 - 05/08/17 09:07 AM (7 years, 8 months ago)

Quote:

bodhisatta said:
Quote:

Hamington Loafburg said:
Cool, Mad.  On agar mycelium runs faster than bacteria, and mold, so another cool trick is to take your transfers before the culture runs into the walls of the plates, transferring away from the very leading edge of the culture with a wedge of culture cut no larger than a grain of rice.  That way the mycelium has a chance to out run any contaminate that may be present.  Once the culture hits the walls, any contaminate that may be hitching a ride will be able to catch up with the mycelium, and the whole culture becomes infected.



Sorry ham I hate to be hard on you but that's some of the worst advice I've seen come out of your mouth.

Molds almost always run faster than mycelium

Molds produce spores as they go so you're letting it sporulate on the plate and contaminate leading edges of "seemingly" clean mycelium at that point

If getting away from mold and bacteria quick rapid transfers are the objective. Never let a plate run, mycelium won't out run molds

Also with contaminated plates you can't use a flow hood use a still air box.

If you have mold you're getting away from if you let that plate grow longer than a day or two the whole surface will be littered with spores so any transfers are playing with fire



This is what RR says to do in the agar section of his videos.  It's what I've been doing for a long time.  :shrug:


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InvisibleTheMadHatter420
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Re: Lucy (Leucistic) Golden Teacher in a 50 Quart Monotub. [Re: bodhisatta]
    #24304225 - 05/08/17 09:08 AM (7 years, 8 months ago)

The plat I transferred from had this kind of mold. Luckily when I transferred the mold in that plate was like 2-3mm wide and pure white still. I don't like the idea of transferring from a mold plate.



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InvisiblebodhisattaMDiscordReddit
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Re: Lucy (Leucistic) Golden Teacher in a 50 Quart Monotub. [Re: TheMadHatter420]
    #24304233 - 05/08/17 09:10 AM (7 years, 8 months ago)

I would be hard pressed to see RR say mycelium runs faster than mold

Hence quick rapid transfers rather than "letting mycelium get ahead of the mold"

Quote:

Hamington Loafburg said:
Cool, Mad.  On agar mycelium runs faster than bacteria, and mold, so another cool trick is to take your transfers before the culture runs into the walls of the plates, transferring away from the very leading edge of the culture with a wedge of culture cut no larger than a grain of rice.  That way the mycelium has a chance to out run any contaminate that may be present.  Once the culture hits the walls, any contaminate that may be hitching a ride will be able to catch up with the mycelium, and the whole culture becomes infected.




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Invisiblewowimflabbergasted
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Re: Lucy (Leucistic) Golden Teacher in a 50 Quart Monotub. [Re: bodhisatta]
    #24304257 - 05/08/17 09:20 AM (7 years, 8 months ago)

Yeah dude myc doesn't grow faster than mold, sorry.


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OfflinehamloafM
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Re: Lucy (Leucistic) Golden Teacher in a 50 Quart Monotub. [Re: wowimflabbergasted]
    #24304312 - 05/08/17 09:46 AM (7 years, 8 months ago)

Yes, specifically in the "Cloning Mushroom Tissue" section at 4:30 seconds.


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InvisiblebodhisattaMDiscordReddit
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Re: Lucy (Leucistic) Golden Teacher in a 50 Quart Monotub. [Re: hamloaf]
    #24304346 - 05/08/17 10:09 AM (7 years, 8 months ago)

Quote:

RogerRabbit said:
  Conidial mycelium grows much faster than dikaryotic mushroom mycelium.
RR



I think it works more like the Qur'an. New information is more important than things said earlier in the timeline

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InvisiblemushboyMDiscord
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Re: Lucy (Leucistic) Golden Teacher in a 50 Quart Monotub. [Re: bodhisatta]
    #24304350 - 05/08/17 10:11 AM (7 years, 8 months ago)

:harhar:

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InvisiblebodhisattaMDiscordReddit
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Re: Lucy (Leucistic) Golden Teacher in a 50 Quart Monotub. [Re: mushboy]
    #24304360 - 05/08/17 10:15 AM (7 years, 8 months ago)

Rely on experience rather than RR said in a video :shrug:

Even though RR actually says repeatedly mold and bacteria will outgrow mycelium if given the time to do so
https://www.shroomery.org/forums/showflat.php/Number/19633525#19633525

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Offlinespacechildo
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Re: Lucy (Leucistic) Golden Teacher in a 50 Quart Monotub. [Re: hamloaf]
    #24304385 - 05/08/17 10:33 AM (7 years, 8 months ago)

Quote:

Hamington Loafburg said:
Yes, specifically in the "Cloning Mushroom Tissue" section at 4:30 seconds.




yeah, he talks about cloning pins specifically, and he never says mycelium grows faster than mold he says myc will start growing out on the dish faster than any possible bacteria or mold spores on the fruit.

it's a completely different scenario.

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OfflinehamloafM
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Re: Lucy (Leucistic) Golden Teacher in a 50 Quart Monotub. [Re: Godseed313]
    #24304397 - 05/08/17 10:38 AM (7 years, 8 months ago)

Quote:

Godseed313 said:


First attempt at agar and my first little ghetto SAB yay! Made up a syringe and knocked up a few 1/2 pints and 2 plates, fingers crossed and I'll check em tonight after work. Been 48 hours so I'm hoping to see some growth.
And plenty Lucy spores set aside just in case I completely screwed up ha I don't want to let Ham down!

Thanks man, you are a gentleman and a scholar, cheers



Awesome, Godseed.  You're going to do great.  Thanks for posting.  Can't wait to see what you do with the genes.


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Invisible13shroomsM
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Re: Lucy (Leucistic) Golden Teacher in a 50 Quart Monotub. [Re: spacechildo]
    #24304667 - 05/08/17 01:11 PM (7 years, 8 months ago)

Quote:

spacechildo said:
Quote:

Hamington Loafburg said:
Yes, specifically in the "Cloning Mushroom Tissue" section at 4:30 seconds.




yeah, he talks about cloning pins specifically, and he never says mycelium grows faster than mold he says myc will start growing out on the dish faster than any possible bacteria or mold spores on the fruit.

it's a completely different scenario.




yup hes talking about this not contams..

ham... :suicide:


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OfflineGodseed313
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Re: Lucy (Leucistic) Golden Teacher in a 50 Quart Monotub. [Re: hamloaf]
    #24304967 - 05/08/17 03:07 PM (7 years, 8 months ago)

Are these generally a fairly speedy colonizer or is that something I'll stumble across after utilizing isolation and transfers on agar? Just wonder because the only strain I've had success with thus far is a mystery strain I started with that colonizes pretty slow but reliably pins like crazy and produces monsters once it gets past colonization. I'm happy to have all I can eat but not knowing what they are drives me up the fucking wall..

My last attempt was TC which I was super excited about but contam'ed like crazy and i wasted the last of that syringe trying to start an LC directly from multispore like a tard...so hoping I can get agar down pat soon and the microwave is only for burritos now :wink:  gonna be super diligent with these precious babies


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