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InvisibleArrakis
PsilocybinPsycho

Registered: 03/03/04
Posts: 276
Scared about inoculation
    #2425139 - 03/12/04 08:50 PM (12 years, 8 months ago)

Hey guys, I'm about to start my first attempt at growing.  I'm using PF Tek & B+ strain.  I'm just scared about contams while I inoculate the jars.  I plan to do so in a large closet which is rarely entered.  Of course I plan to sterilize the shelves on which I will be working, am going to take a shower beforehand, wear gloves/mask/hat, sterilize needle w/ 91% achohol & flame, and will being working wearing only my boxers.  What do you guys think?

Should I be this frightened of contams while inoculating?  Is my only real concern of contams, after sterilizing jars in a PC, the needle when it passes through the vermiculite layer?

I'm looking for a little reassurance/tips about sterility of this plan.  Thanks in advance for your thoughts!  :biggrin:


--------------------
Funniest shit I've read in a while:
http://www.shroomery.org/forums/showflat.php?Cat=&Number=886829&page=&view=&sb=5&o=&fpart

"He who controls the spice controls the universe!"


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InvisiblePrisoner#1M
Even Dumber ThanAdvertized!
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Registered: 01/22/03
Posts: 191,639
Loc: Pvt. Pubfag NutSuck
Trusted Cultivator
Re: Scared about inoculation [Re: Arrakis]
    #2425143 - 03/12/04 08:52 PM (12 years, 8 months ago)

you'll be fine, you seem to have a great grip on it...


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Anonymous

Re: Scared about inoculation [Re: Arrakis]
    #2425344 - 03/12/04 10:21 PM (12 years, 8 months ago)

wow thats over doing it a ton


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InvisibleArrakis
PsilocybinPsycho

Registered: 03/03/04
Posts: 276
Re: Scared about inoculation [Re: fee]
    #2425364 - 03/12/04 10:28 PM (12 years, 8 months ago)

Thanks guys, I feel alot better now. :laugh:  It's funny, but fee's comment makes me feel REALLY confident.  :thumbup:


--------------------
Funniest shit I've read in a while:
http://www.shroomery.org/forums/showflat.php?Cat=&Number=886829&page=&view=&sb=5&o=&fpart

"He who controls the spice controls the universe!"


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Anonymous

Re: Scared about inoculation [Re: Arrakis]
    #2425388 - 03/12/04 10:35 PM (12 years, 8 months ago)

ya man just flaming the needle and whiping with alcohol and a quick move to the jar/bag is all you need.
the biggest thing is to make sure your substrate is sterile thats the hardest part
and its not that hard


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Invisiblesakura
Aussie Expat

Registered: 02/08/04
Posts: 592
Loc: Japan
Re: Scared about inoculation [Re: Arrakis]
    #2426040 - 03/13/04 02:44 AM (12 years, 8 months ago)

Quote:

Arrakis said:
... and will being working wearing only my boxers...




Do it naked (or wear a latex Superman suit...)

And post Photos (makes for an interesting grow-log anyway)
:lol: :lol: :lol:


--------------------
Shrooms aren't everyone's cup of tea... (Some folks just eat 'em)


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InvisibleArrakis
PsilocybinPsycho

Registered: 03/03/04
Posts: 276
Re: Scared about inoculation [Re: sakura]
    #2426170 - 03/13/04 04:13 AM (12 years, 8 months ago)

I can't do it naked, I never know when a pube might fly into the air & onto the syringe.  I was wondering if anyone even noticed that part about the boxers...  :smile:


--------------------
Funniest shit I've read in a while:
http://www.shroomery.org/forums/showflat.php?Cat=&Number=886829&page=&view=&sb=5&o=&fpart

"He who controls the spice controls the universe!"


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OfflineFirstAvailable
enthusiast
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Registered: 02/14/04
Posts: 728
Last seen: 2 years, 2 months
Re: Scared about inoculation [Re: Arrakis]
    #2430622 - 03/14/04 08:05 AM (12 years, 8 months ago)

Quote:

Arrakis said:
I can't do it naked, I never know when a pube might fly into the air & onto the syringe.  I was wondering if anyone even noticed that part about the boxers...  :smile:




Make sure to tape up the "frontal opening" on the boxers.  Those pesky pubes have a mind of their own, ya know.
:wink:


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Offlineteen
Yeee Haaww

Registered: 02/13/04
Posts: 242
Last seen: 10 years, 9 months
Re: Scared about inoculation [Re: FirstAvailable]
    #2430662 - 03/14/04 09:09 AM (12 years, 8 months ago)

I hope you've clogged up all your body's openings with polyfill, you never know when some stray contams will come out.


--------------------
Don't give me that load of bunk~!


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Offlinetralfamadorian
AspiringMycologist
Registered: 03/09/04
Posts: 25
Last seen: 12 years, 8 days
Re: Scared about inoculation [Re: teen]
    #2431870 - 03/14/04 06:24 PM (12 years, 8 months ago)

tar should work


--------------------
"So maybe the whole thing does happen inside one's skull. Maybe it is private and there's no unitive knowledge of anything but one's own physiology. Who cares? The fact remains that the experience can open one's eyes and make one blessed and transform one's whole life."


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OfflineEddieRebel
Day Tripper
Registered: 03/14/04
Posts: 18
Last seen: 1 year, 9 months
Re: Scared about inoculation [Re: FirstAvailable]
    #2432156 - 03/14/04 08:08 PM (12 years, 8 months ago)

The easiest solution would be to either shave your pubes, or just rip em out. Also check the taint/anus for stray hairs, they can be pesky.


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OfflineGlobeOfFrogs
Stranger
Registered: 02/29/04
Posts: 14
Last seen: 6 years, 10 months
Re: Scared about inoculation [Re: EddieRebel]
    #2432168 - 03/14/04 08:12 PM (12 years, 8 months ago)

I like to dip myself in wax prior to innoculation.


Just for fun!


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OfflineChaotica
outsider
Registered: 11/16/02
Posts: 14
Loc: Pacific Northwest
Last seen: 12 years, 7 months
Re: Scared about inoculation [Re: Arrakis]
    #2432937 - 03/15/04 12:04 AM (12 years, 8 months ago)

I hope it went well.
I took roughly the same precautions you did and I innoculated twelve jars with one hundred percent success, first time, too.
Granted, they took over a month to colonize (seattle winter!), but zero contamination completely rocked my world.


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InvisibleArrakis
PsilocybinPsycho

Registered: 03/03/04
Posts: 276
Re: Scared about inoculation [Re: Chaotica]
    #2433304 - 03/15/04 01:26 AM (12 years, 8 months ago)

I'm not really worried about my damned pubes!  :shake:
Oh well.

I just didn't understand why some people who took precautions with PF jars got contams, and didn't want it to happen to me my first time around.  Thanks for all the attention though.  :cool:


--------------------
Funniest shit I've read in a while:
http://www.shroomery.org/forums/showflat.php?Cat=&Number=886829&page=&view=&sb=5&o=&fpart

"He who controls the spice controls the universe!"


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Invisibletoad857
President of theUnited States

Registered: 02/02/04
Posts: 283
Re: Scared about inoculation [Re: Arrakis]
    #2452014 - 03/19/04 05:34 PM (12 years, 8 months ago)

what a funny thread


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OfflineMinimumEfrt2
enthusiast
Registered: 03/17/04
Posts: 293
Last seen: 6 years, 3 months
Re: Scared about inoculation [Re: fee]
    #2452774 - 03/19/04 09:44 PM (12 years, 8 months ago)

R u saying that RIGHT after u flame the needle u inoculate? wouldnt that damage the spores??


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InvisiblePrisoner#1M
Even Dumber ThanAdvertized!
 User Gallery

Registered: 01/22/03
Posts: 191,639
Loc: Pvt. Pubfag NutSuck
Trusted Cultivator
Re: Scared about inoculation [Re: MinimumEfrt2]
    #2453060 - 03/20/04 12:12 AM (12 years, 8 months ago)

the water that initialy comes out will cool the needle, I usualy give 10 seconds to cool


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Offlinemenlatin
Stranger
Registered: 02/05/04
Posts: 5
Last seen: 12 years, 6 months
Re: Scared about inoculation [Re: fee]
    #2454057 - 03/20/04 01:18 PM (12 years, 8 months ago)

heh, me and my cousin are just starting, and we made a room in his kitchen of plastic film, and bleached and alchoholed the inside, alchoholed our bodies and clothes, face masks, plastic bags on our heads. think thats a little over kill?


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Amazon Shop for: ½ Pint Jars, Vermiculite

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