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OfflineNoahr66
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G2G with tiny Myc pebbles
    #24250056 - 04/16/17 08:18 PM (7 years, 8 months ago)

hey, I've been posting about the status of 3 different grows on one outdated question thread, so thought I'd post here the details of one I think could be useful for anyone interested. After looking through the forums on G2G I saw that everyone said to use a lot of spawn for G2G, But then I came across a reply to a post on g2g ratios where a TC (can't remember who) said "technically one piece of colonized grain should be enough to colonize a whole jar". So I used literally that; One tiny gravel sized piece of colonized BRF cake for each jar.

The presence of a bacterial contaminate on the BRF cake was one issue, and may still be an issue, but I used the healthiest looking myc i could find on it. I know someone else said they've done G2G with questionable spawn and have success. I cut the pieces off before the transfer, and put the chunks in a sterile rubbermaid container until transferring.

I soaked and pressure cooked the WBS as any tek calls for, then sterilized the work area and did the transfers in a SAB, everything wiped down with alcohol and everything. with a knife I dropped the mycelium down into the jars as fast as possible, and because of forgetting to put any air circulation on the lids, there was a major vacuum seal when I took off each lid.

BUT the good news, is that on 3 of the 4 jars I see definite rhyzomorhpic mycelium poking through the WBS. two were visible enough to get on a picture, but one was just a few hair strands looking growth down in the crevice. It will probably be visible tomorrow. I used 1 1/2 pint jar and 2 full pint. When I was shaking the jars some of the verm on the bottom got mixed throughout the seed, but I don't see why that would be a problem. I'll post pictures of them currently, And keep it updated.

My only question would be why is the recommended spawn to grain rate not simply "1 piece of colonized grain" instead of the 1-5/1-10 spawn to grain ratio I always see? Since I started with such small pieces of colonized grain is there a chance it will be a weaker substrate once its finished, or have a bigger chance of stalling and lower yields? I haven't seen much of anything about this.


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InvisiblemushboyMDiscord
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Re: G2G with tiny Myc pebbles [Re: Noahr66]
    #24250090 - 04/16/17 08:33 PM (7 years, 8 months ago)

did you mod the lids? they shouldnt have a vacuum. alot of dirty air can be sucked inside the jar.

edit:oops helps to double read. that might cause issues...


its a numbers game. more inoculation points the quicker the colonizing.
ive never done g2g with a single grain but ive had jars with a few clumped up
in the bottom of a master jar, so since it was in the sab i poured more grains
into the empty jar with the clump and it colonized fine. but i wouldnt do it again. seems risky

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Re: G2G with tiny Myc pebbles [Re: mushboy]
    #24250099 - 04/16/17 08:37 PM (7 years, 8 months ago)

When you were shaking the jars, some of the verm got mixed in with the seed.......
What is the verm from? The piece you cut off?


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OfflineNoahr66
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Re: G2G with tiny Myc pebbles [Re: mushboy]
    #24250109 - 04/16/17 08:40 PM (7 years, 8 months ago)

I didn't, I read somewhere someone said that you don't want airflow during colonization, because the co2 stimulates growth so i kept it airtight. After already dropping the myc in I was reading and saw someone saying that airflow is crucial to mycelium growth. Nothing I can do now without opening up a door to contaminates though.

I heavily sprayed the room with lysol before and did everything in a SAB, and everything even nearby my work area was wiped down with alcohol so It would be reasonable to believe that whatever air it pulled in though from the vacuum seal could've been sterile air.

Before even starting sterilizing anything I opened all the windows in the room for a few hours so the air would be fresh and less full of contaminate spores. Of course I closed them before Spraying and sterilizing hard surfaces.

Edited by Noahr66 (04/16/17 10:35 PM)

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OfflineNoahr66
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Re: G2G with tiny Myc pebbles [Re: Pipefitter537]
    #24250118 - 04/16/17 08:44 PM (7 years, 8 months ago)

Quote:

Pipefitter537 said:
When you were shaking the jars, some of the verm got mixed in with the seed.......
What is the verm from? The piece you cut off?




No, I put dry vermiculite in the bottom of the jars so any excess moisture would be soaked up by it. the chunks of BRF cake used were small enough to contain maybe one full peice of vermiculite each.


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OfflineNoahr66
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Re: G2G with tiny Myc pebbles [Re: Noahr66]
    #24251964 - 04/17/17 03:46 PM (7 years, 8 months ago)

http://
http://

two pictures I took last night, and It's even more thick today! aside from those 2 jars,  one jar still has no noticeable growth, and one has one tiny patch that hasn't grown too much. If I can Get even one healthy grain spawn jars out of a contaminated BRF cake I'll be more than happy though.

I think this Would be a great idea to tell people about though who have an unhealthy cake, if it works out; a lot of people would have advised me "just throw it away"!


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InvisiblemushboyMDiscord
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Re: G2G with tiny Myc pebbles [Re: Noahr66]
    #24252047 - 04/17/17 04:30 PM (7 years, 8 months ago)

???

verm layers in a grain jar?
:whattefuck2:
Quote:

Noahr66 said:
I didn't, I read somewhere someone said that you don't want airflow during colonization, because the co2 stimulates growth so i kept it airtight. After already dropping the myc in I was reading and saw someone saying that airflow is crucial to mycelium growth. Nothing I can do now without opening up a door to contaminates though.

I heavily sprayed the room with lysol before and did everything in a SAB, and everything even nearby my work area was wiped down with alcohol so It would be reasonable to believe that whatever air it pulled in though from the vacuum seal could've been sterile air.

Before even starting sterilizing anything I opened all the windows in the room for a few hours so the air would be fresh and less full of contaminate spores. Of course I closed them before Spraying and sterilizing hard surfaces.




damn.. why would you open a window? a SAB is about still air. the air itself can be dirty as fuck but its still.
all opening a window does PRIOR to SAB work is stir up air. and outside air has way more spores than stale inside air.

the lysol and SAB work sound ok. glad you used one:thumbup: remember, you are sanitizing hard surfaces.. not sterilizing..
i know it can seem like its spitting hairs but knowing the difference is critical to learning more techniques/know
what people are talking about... i didnt know for awhile what the difference was...dont be like me.

where did you hear that shit about airflow during colonizing.???

keep asking questions. virginia is for lovers.

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OfflineNoahr66
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Re: G2G with tiny Myc pebbles [Re: mushboy]
    #24252080 - 04/17/17 04:46 PM (7 years, 8 months ago)

I closed the window and closed all the AC vents long before sterilizing every thing. The air was so still in there I started sweating bad while doing the transfer. some from nerves Im sure though lol. I just got it to where the air that is still is at least more clean, so should there be an accident such as a vacuum seal sucking some air in, whatever air that's pulled in is less likely to harbor contaminants.


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OfflineNoahr66
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Re: G2G with tiny Myc pebbles [Re: Noahr66]
    #24252108 - 04/17/17 04:56 PM (7 years, 8 months ago)

didn't know there was a real difference between sterilizing and sanitizing honestly


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OfflineNoahr66
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Re: G2G with tiny Myc pebbles [Re: Noahr66]
    #24252112 - 04/17/17 05:00 PM (7 years, 8 months ago)

The verm layer on the bottom was suggested in someones tek I read through for WBS (other grain would work too) called the no fail tek or something along the lines. It was made mostly for new people who might not be sure about water content, so there verm on the bottom soaks up excess moisture if there is any. I did that just to be safe, but I got it right on my own it looked like so I probably will try it without in the future.


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Re: G2G with tiny Myc pebbles [Re: Noahr66]
    #24252565 - 04/17/17 07:23 PM (7 years, 8 months ago)

not to shovel my own shit but click making birdseed in my links. its easy as fuck to do a basic run.:thumbup:

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OfflineNoahr66
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Re: G2G with tiny Myc pebbles [Re: mushboy]
    #24253830 - 04/18/17 08:43 AM (7 years, 8 months ago)

http://

http://

around 33 hours later than the first pictures. its growing fast! the other two jars haven't changed.  no possible contaminate on the clear jar right? i think its just thin mic, but it looks somewhat.. cobwebby.


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Re: G2G with tiny Myc pebbles [Re: Noahr66]
    #24254004 - 04/18/17 09:59 AM (7 years, 8 months ago)

yah that aint good. might be pin mold? but thats gonna get shitty:thumbdown:

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OfflineNoahr66
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Re: G2G with tiny Myc pebbles [Re: mushboy]
    #24255424 - 04/18/17 08:50 PM (7 years, 8 months ago)

don't think its pin mold though because theres no black spots. I think the seed might just be dry; it looks more white than the picture makes it look.


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Re: G2G with tiny Myc pebbles [Re: Noahr66]
    #24255428 - 04/18/17 08:53 PM (7 years, 8 months ago)

i grew the same shit. tis not myc


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OfflineNoahr66
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Re: G2G with tiny Myc pebbles [Re: mushboy]
    #24257243 - 04/19/17 12:46 PM (7 years, 8 months ago)

Is there any way that pin mold could be on all 3 jars though? there's 3 growing pretty well now and its starting to get more solid in areas. It looks like the same type of thing on each one


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OfflineNoahr66
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Re: G2G with tiny Myc pebbles [Re: Noahr66]
    #24257332 - 04/19/17 01:12 PM (7 years, 8 months ago)

looked it up and saw what myc's supposed to look like.. guess its not myc. At least Ive still got the Sclerotia jars


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OfflineNoahr66
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Re: G2G with tiny Myc pebbles [Re: Noahr66]
    #24257411 - 04/19/17 01:41 PM (7 years, 8 months ago)

That doesn't look like pin mold though, It looks more like cobweb. But cobweb Is described as having longer more fluffed out hairs. Mine aren't long at all and not all that fluffy, more fluffy as in consolidating some and getting more white. It's very likely it's cobweb but I'm going to give it another day. I saw a thread where a guys told he has cobweb, but it was just stringy myc growth because he gave it some time first before throwing it out.


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Re: G2G with tiny Myc pebbles [Re: Noahr66]
    #24257435 - 04/19/17 01:54 PM (7 years, 8 months ago)

Awe dang man. Always stings a little when the molds gets ya.
What's it look like now?

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OfflineNoahr66
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Re: G2G with tiny Myc pebbles [Re: mynakedrat]
    #24257447 - 04/19/17 02:04 PM (7 years, 8 months ago)

I shook it this morning actually cause I thought It was myc, but just more solid looking and a little more growth. It's also not growing extremely rapidly though. I inoculated with the spawn over a week ago I believe. This growth has been slowly taking place over the course of 3 days, and Its only in one spot. Also Mold flourishes on over wet substrates but the WBS like I think I said earlier Is exceptionally dry. when I was shaking it it felt like I was shaking plain hard seeds. I soaked for 18 hours, simmered for 30 minutes, and then let it dry so IDK what I did wrong with that. Maybe It looks somewhat weird because of that. I'm probably just trying to make up reasons o why i shouldn't throw it out now but. maybe :shrug:


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