Home | Community | Message Board


Mushrooms.com
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Contamination Forum

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop for: ½ Pint Jars, Vermiculite

Jump to first unread post. Pages: 1
OfflineM2ThaG2000
Stranger
Registered: 03/09/04
Posts: 1
Last seen: 12 years, 11 months
I don't know what's going on!!
    #2424913 - 03/12/04 06:52 PM (12 years, 11 months ago)

I innoculated 12 PF jars last monday. Everything was going fine. But after a few days...green mold. So out they go. Mix new verm and re-sterilize jars. Innoculate. 3 days later...green mold! What's going on? I've done this before in the exact same place without problems. Could it be that the spores are contaminated? Please help.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineExtravagantDream
Beacon in theDarkness
 User Gallery

Registered: 10/24/02
Posts: 1,271
Loc: Somewhere in the Local Su...
Last seen: 9 years, 8 months
Re: I don't know what's going on!! [Re: M2ThaG2000]
    #2424943 - 03/12/04 07:08 PM (12 years, 11 months ago)

What is your sterilization method?
Where and how do you innoculate?
If you bought the syringe off of one of the many spore companies it is Very doubtful it is from the spore solution.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineSuntzu
Geek
 User Gallery

Registered: 10/15/99
Posts: 1,394
Last seen: 12 days, 15 hours
Re: I don't know what's going on!! [Re: M2ThaG2000]
    #2425052 - 03/12/04 08:13 PM (12 years, 11 months ago)

If you're doing 12 at once, it would be a good idea to incorporate a couple of negative controls. Take two jars fresh from the PC; Set one aside to incubate with the rest. 'Fake innoculate' the second one, where you do everything you would with an innoculation, but don't expel any spore solution.
These two jars can give you a good idea of the source.
One recommendation, be sure to cool the PC in a clean place; Molds are not likely to survive PC'ing, and are more often introduced during the cooling or innoculation stage.
It could be that the spores are contaminated, it's not out of the question. If both control jars do not contaminate while the rest go bad, that will point towards the innoculum. It might be a dirty needle/anything related to the knock-up process, if only the second control jar contaminates. It's not a guaranteed answer, but should give some insight.


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop for: ½ Pint Jars, Vermiculite

Mushrooms, Mycology and Psychedelics >> Contamination Forum

Similar ThreadsPosterViewsRepliesLast post
* Don't think it's cobweb, but..... magicfungi 1,431 9 05/20/03 06:09 PM
by Dank420
* PF jar contams twice (now 3) in a row. upupup 3,197 17 05/26/02 08:22 AM
by baraka
* If Ya See Mold and then Sterilize Jars will it... NutzLunch 864 4 05/01/02 06:15 AM
by luvdemshrooms
* Newly innoculated jars now smell like dead rats?? havatampa 666 2 02/10/04 12:30 PM
by MAIA
* Smelt Jars for contamination. I was taken to ER!!!
( 1 2 3 4 5 6 all )
JayBin 18,924 104 04/21/03 06:20 PM
by Ekstaza
* Rusty looking contam on pre-innoculated jars???Help! harken989 909 7 05/12/03 08:08 PM
by apuffball
* GREEN MOLD ON ALL MY JARS, HELP!!!!!! Supershrooomer 2,053 4 11/21/01 02:47 AM
by TeRzMaStA
* Baeocystis Jars - Any experienced Baeo cultivators know if this is contamination or normal??
( 1 2 all )
mushroomhunter10 2,788 29 06/04/09 09:55 AM
by mushroomhunter10

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, cronicr, Tmethyl, Pastywhyte
549 topic views. 1 members, 3 guests and 0 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Azarius
Please support our sponsors.

Copyright 1997-2017 Mind Media. Some rights reserved.

Generated in 0.026 seconds spending 0.006 seconds on 14 queries.