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Amazon Shop: ½ Pint Jars, Vermiculite

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Registered: 03/09/04
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I don't know what's going on!!
    #2424913 - 03/12/04 06:52 PM (13 years, 5 months ago)

I innoculated 12 PF jars last monday. Everything was going fine. But after a few days...green mold. So out they go. Mix new verm and re-sterilize jars. Innoculate. 3 days later...green mold! What's going on? I've done this before in the exact same place without problems. Could it be that the spores are contaminated? Please help.

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Re: I don't know what's going on!! [Re: M2ThaG2000]
    #2424943 - 03/12/04 07:08 PM (13 years, 5 months ago)

What is your sterilization method?
Where and how do you innoculate?
If you bought the syringe off of one of the many spore companies it is Very doubtful it is from the spore solution.

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Re: I don't know what's going on!! [Re: M2ThaG2000]
    #2425052 - 03/12/04 08:13 PM (13 years, 5 months ago)

If you're doing 12 at once, it would be a good idea to incorporate a couple of negative controls. Take two jars fresh from the PC; Set one aside to incubate with the rest. 'Fake innoculate' the second one, where you do everything you would with an innoculation, but don't expel any spore solution.
These two jars can give you a good idea of the source.
One recommendation, be sure to cool the PC in a clean place; Molds are not likely to survive PC'ing, and are more often introduced during the cooling or innoculation stage.
It could be that the spores are contaminated, it's not out of the question. If both control jars do not contaminate while the rest go bad, that will point towards the innoculum. It might be a dirty needle/anything related to the knock-up process, if only the second control jar contaminates. It's not a guaranteed answer, but should give some insight.

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Amazon Shop: ½ Pint Jars, Vermiculite

Mushrooms, Mycology and Psychedelics >> Contamination Forum

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