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OfflineNoahr66
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Pins on brf cakes haven't grown in 9 days, some pins have receded; yellowish hue to mycelium.
    #24237635 - 04/12/17 02:07 PM (7 years, 9 months ago)

hello. If you've seen my recent posts, disregard them, In the last week or so I've been scanning the archives for 2-5 hours a day for any information I could find, so most of my questions have been answered and then some.
*As a sidenote, I don't think it's paid enough attention to that the process of growing (or trying and failing to grow) the mushrooms is a considerable experience of itself, not counting the actual end product. It's been extremely interesting, somewhat humbling, and at many times exciting (first myc growth, first pins).

I'll try not to waste anyones time with too much info, but I'll give you a short summary of everything up to this point; Sometime in early Feb I inoculated 4 1/2 pint jars with golden teacher spores from freespores. they grew very slowly, took over a month to fully colonize. 1 never grew anything after nearly a month, 1 had colonized half the jar and then got some red spot cobweb looking growth throughout the other half. thrown out. 1 was in a badly made fc and got a grey mold over half of it. coated it with peroxide but it kept coming back (and the mycelium looked basically dead, got bad advice at how much peroxide to use). so thrown out. The one still alive pinned in vitro after full colonization and then was moved to an fc. Up to this point I've made embarrassingly stupid mistakes, such as giving no air circulation to the first cake (likely what resulted in the mold), resting cakes on verm in fc instead of aluminum foil, pouring water on cakes instead of misting, and an assortment of other foolish mistakes I can't remember.

At this point I've cleaned up my technique (imo) in most ways. But I am still having some problems, likely from my past cutting corners self. first of all, the pins in vitro weren't so much "pins" as they were caps. There has been no growth in the last few days, and some even aren't visible anymore; its as if they've receded back int the cake!There is an abundance of metabolites on one side with less pins, and the mycelium is a yellowish hue in areas. another problem is that the humidity won't rise above 80-85. temp stays between 68- 80. What could be the reason for them stopping growing and the off color and are these pins done? I know many people would say "throw it out" but I got so close.. Even if its a bacterial infection i know I can still move it outdoors and fruit it. which i what I'd do if the pins didn't stop growing.

I've already ordered some mexicans spores for sclerotia in WBS, and as an EXPERIMENT, I'm not expecting much, a couple days ago I PCed some WBS jars with verm on the bottom, 1 1/2 pint, 3 full pints. In an alcohol sterilized glove box I shaved 4 small pieces off the whitest looking parts of the brf cake, and put them in the wbs jars in the glove box. They were small pieces as I didn't want to do too much damage to the cake, it was basically a last ditch effort on golden teachers should the cake not work out. since they were small I'd expect that means if it does grow it would be very slowly.

thank you for any help or pointers, I occasionally also point a fan towards the fc with a little distance and for a couple hours I'll open the window its next to, and let the sunlight get on it. RR said cubes grow in open fields in one post and sunlight won't damage them in one post, I'll find it if you don't trust me. I'm currently still using paper towels in the bottom instead of perlite, but I plan to go get perlite today.

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OfflineNoahr66
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Re: Pins on brf cakes haven't grown in 9 days, some pins have receded; yellowish hue to mycelium. [Re: Noahr66]
    #24237697 - 04/12/17 02:28 PM (7 years, 9 months ago)

http://
6 days ago.

http://
directly after coming out of the jar.

http://
now


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OfflineNoahr66
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Re: Pins on brf cakes haven't grown in 9 days, some pins have receded; yellowish hue to mycelium. [Re: Noahr66]
    #24237723 - 04/12/17 02:36 PM (7 years, 9 months ago)


fruiting chamber

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Offlinejusthavefun
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Re: Pins on brf cakes haven't grown in 9 days, some pins have receded; yellowish hue to mycelium. [Re: Noahr66]
    #24237737 - 04/12/17 02:39 PM (7 years, 9 months ago)

They look dry (Hard to Tell from pictures) imo. Perlite is a must, did you dunk them? Id guess not enough water is the main problem, however, im a newbie myself. Also your "pins" look quite strange, never seen sth similar before.


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Invisiblepetey mcenroe
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Re: Pins on brf cakes haven't grown in 9 days, some pins have receded; yellowish hue to mycelium. [Re: justhavefun]
    #24237796 - 04/12/17 02:56 PM (7 years, 9 months ago)

Ya gotta build a proper SGFC to get it to work right. Get a much larger tub with about 4-5 inches of perlite that has been rinsed. Also gotta dunk n roll those cakes. Just follow the teks to the T an you can't go wrong. I'm also a newb, but all my grows produced shrooms so far. Yeah I've had contamination issues on a few cakes, but that is expected from a newb.


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wussup baby i have sumthin' for your sweet tooth

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OfflineVesta
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Re: Pins on brf cakes haven't grown in 9 days, some pins have receded; yellowish hue to mycelium. [Re: Noahr66]
    #24237828 - 04/12/17 03:11 PM (7 years, 9 months ago)

There are no pins present in those pictures. Super bacterial cakes though.


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"It's really hard to fuck up a cubensis grow" - RogerRabbit


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OfflineNoahr66
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Re: Pins on brf cakes haven't grown in 9 days, some pins have receded; yellowish hue to mycelium. [Re: Vesta]
    #24238654 - 04/12/17 08:26 PM (7 years, 9 months ago)

well damn. if those aren't early stage shroom what are they out of curiosity? I scanned the archives for something similar to what I have and found nothing.

I was going to get perlite today, but thought id ask here whether its worth it first, as I'll never do cakes again and perlite would be useless except for this one use.

I remember a post where RR said Paper towels will work, so thats why I decided to just use paper towels.

at this point is it safe to assume that it won't survive in the fc or does it still have a good chance? If not I was going to put it outside. If put outside should I mix with coco coir or just put it straight in a hole? Don't know if the bacteria would take over the coir instead of myc. Either way i was planning on putting a coat of verm on the top and bottom of the hole (and maybe on the sides) for humidity. The verm on the bottom would soak up excess moisture, while the coat on top would drop down moisture If it gets dry.


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Edited by Noahr66 (04/12/17 09:20 PM)

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Invisiblemynakedrat
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Re: Pins on brf cakes haven't grown in 9 days, some pins have receded; yellowish hue to mycelium. [Re: Noahr66]
    #24238864 - 04/12/17 10:00 PM (7 years, 9 months ago)

What are those standing in? Sponges?
That's no good.
Cakes look like they are fighting off bacteria and loses. Look like sores about to pop.
There's no verm on the cakes anymore, and I see no perlite
If anything you're gonna get some blumpkins.
Everyone with the blumpkin blobs today.
Strange...

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OfflineGunnar
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Re: Pins on brf cakes haven't grown in 9 days, some pins have receded; yellowish hue to mycelium. [Re: mynakedrat]
    #24239137 - 04/13/17 12:31 AM (7 years, 9 months ago)

Look up the SGFC tek, your FC is the problem.

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OfflineNoahr66
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Re: Pins on brf cakes haven't grown in 9 days, some pins have receded; yellowish hue to mycelium. [Re: Gunnar]
    #24239157 - 04/13/17 12:52 AM (7 years, 9 months ago)

I changed a lot of stuff throughout the pictures. its now resting on aluminum foil and I mist the walls 3 times a day, fan 5-7. the humidity is at 88 rn. whats wrong with the fc other than paper towels instead of perlite? At this point is it salvageable?


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OfflineNoahr66
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Re: Pins on brf cakes haven't grown in 9 days, some pins have receded; yellowish hue to mycelium. [Re: Gunnar]
    #24239160 - 04/13/17 12:57 AM (7 years, 9 months ago)

Quote:

Gunnar said:
Look up the SGFC tek, your FC is the problem.




I see now what you're saying with the fc, Is it just too small then and not deep enough? for one cake I thought it would make sense to be suitable to a smaller version of the sgfc. another newb mistake I guess. Thanks for pointing that out though


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OfflineGunnar
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Re: Pins on brf cakes haven't grown in 9 days, some pins have receded; yellowish hue to mycelium. [Re: Noahr66]
    #24239179 - 04/13/17 01:13 AM (7 years, 9 months ago)

You need a clear tote, holes on ALL 6 SIDES!!! About 5 inches of perlite, and it also has to be elevated at least 3 inches, more doesn't hurt. I put my sgfc on top of some jars to keep it elevated. It also can't be too close to the wall. All the holes and keeping it elevated will increase free air exchange which will induce pinning. The air will flow through the bottom and out the top when set up properly.

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OfflineGunnar
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Re: Pins on brf cakes haven't grown in 9 days, some pins have receded; yellowish hue to mycelium. [Re: Gunnar]
    #24239183 - 04/13/17 01:17 AM (7 years, 9 months ago)

I would keep your fc on that green tote it's on but just put like 4 pint jars under it to keep it elevated, also I'm not too sure about your cake but my gut says it's fine considering your improper conditions (no offense) I think the discoloration is just metabolites/cries for help.

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OfflineNoahr66
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Re: Pins on brf cakes haven't grown in 9 days, some pins have receded; yellowish hue to mycelium. [Re: Gunnar]
    #24239220 - 04/13/17 01:52 AM (7 years, 9 months ago)

Quote:

Gunnar said:
I would keep your fc on that green tote it's on but just put like 4 pint jars under it to keep it elevated, also I'm not too sure about your cake but my gut says it's fine considering your improper conditions (no offense) I think the discoloration is just metabolites/cries for help.



None taken. Looking back on some of the mistakes I made, It frustrates me that I went about things so carelessly in parts. Since I thought The cake was pinning I skipped dunk n roll, but since it is in fact not pinning, I went ahead with dunking. I've seen teks that say to dunk for 10 minutes in 1-200 bleach-water solution and It will kill bacterial contamination. People say they've had great success with it. Not sure about it though because bad advice about 2% peroxide killed all the myc on a contaminated cake that could've easily still made it outside. The jar was incubating from feb 22- april 3rd. not sure If it was bad technique or bad spores but it took 40? days for the jar to completely colonize. Sure that left it pretty dry now that I actually did the math on just how long it actually took.


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OfflineNoahr66
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Re: Pins on brf cakes haven't grown in 9 days, some pins have receded; yellowish hue to mycelium. [Re: Noahr66]
    #24239225 - 04/13/17 01:55 AM (7 years, 9 months ago)

If it gets better, I'll fruit inside. If not, Ill end up just moving it outdoors. since Its only 1 cake though I've been thinking about casing for maximizing yields. Not too much, 1-.75 spawn- bulk substrate ratio. just enough to make it worth it but not so much that It will make it an extreme challenge for the mac to colonize coir. if that would be too risky though I'll just fruit in the fc.


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OfflineGunnar
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Re: Pins on brf cakes haven't grown in 9 days, some pins have receded; yellowish hue to mycelium. [Re: Noahr66]
    #24239233 - 04/13/17 02:03 AM (7 years, 9 months ago)

You should use regular tap water (cold) to dunk, keep it submerged 12-24 hours, also read the amu teks thread that is stickied

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InvisibleZiran
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Re: Pins on brf cakes haven't grown in 9 days, some pins have receded; yellowish hue to mycelium. [Re: Noahr66]
    #24239253 - 04/13/17 02:51 AM (7 years, 9 months ago)

Updated Pf-tek guide

This is the recommended pf-tek. Its even on the main site page of the shroomery.


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Song Of Healing
:super: Updated Pf Tek Guide :super:
Ziran's Teks
AMU Q&A Thread
The Chinese word for nature is zìrán and it means that of which is of itself.


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OfflineNoahr66
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Re: Pins on brf cakes haven't grown in 9 days, some pins have receded; yellowish hue to mycelium. [Re: Ziran]
    #24241076 - 04/13/17 05:29 PM (7 years, 9 months ago)

Quote:

Ziran said:
Updated Pf-tek guide

This is the recommended pf-tek. Its even on the main site page of the shroomery.



thanks, read through it but I think since this is the last I have though I'm going to do more with it than a standard pf tek calls for though. I dunked for about 12-13 hours, then put a 1-250/300 (not measured 100% accurately) bleach water for 10 minutes. I took it out, rinsed in fresh water, then rolled in vermiculite. The verm was damp but definitely not wet no drops came out when I squeezed it. I piled it on there as thick as I could. from here I'll probably fruit it a time or two, then use it for g2g, and then case in coir. Thought about doing the "Fat Bag Tek" maybe, but I definitely want to fruit it normally at least once before doing this. Got perlite, a 5000 k CFL ( no stores i went to had 6500) but no new tote yet. plan to get that tomorrow along with RGS for the mexicana syringe arriving saturday.

from what Ive seen though it seems the mix of FAE, humidity, and evaporation is the number 1 pinning trigger, along with maybe light. so tell me if this is right or wrong; since it jut got out of the dunk and is already saturated wouldn't giving it abundant airflow ( such as pointing a fan towards the fc) at this stage trigger more pinning than after the surface moisture from the dunk is gone? I put perlite in the tote I already have for now but will change it out tomorrow.


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Edited by Noahr66 (04/13/17 05:41 PM)

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OfflineNoahr66
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Re: Pins on brf cakes haven't grown in 9 days, some pins have receded; yellowish hue to mycelium. [Re: Noahr66]
    #24241096 - 04/13/17 05:37 PM (7 years, 9 months ago)

BTW with the four WBS jars Imoved some myc to, since the cake was bacterial is there any chance of the make in the jars beating the bacteria and colonizing the wbs? I know I saw somehwere on here saying that G2G transfers are still able to be done in the present doc bacteria. I chose myc from the whitest, healthiest areas I could find. small pieces though, which I know will lead to long colonization time, but I read somewhere that 1 piece of colonized grain will still colonize, just slowly. There was a lot of moisture inside the jars the first day after pressure cooking, but now they appear dry. no visible moisture at all. I put verm i the bottoms to soak up excess moisture, but my fear is that it soaked up too much moisture. I shook the jars right after taking them out of the pc. The jars don't have any airflow because one person said CO2 stimulates micx growth. So I made no air circulation. after id already inoculated, I saw someone say THE EXACT OPPOSITE. That seems to be an extremely common occurrence on this forum though.


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OfflineNoahr66
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Re: Pins on brf cakes haven't grown in 9 days, some pins have receded; yellowish hue to mycelium. [Re: Noahr66]
    #24241190 - 04/13/17 06:23 PM (7 years, 9 months ago)

nice avatars @Zran and gunnar btw, only game I could ever bring myself to play while tripping was majoras mask. one of the best games I ever played, it never seems to get old. Crazy deep game and story background too if you look deeper into it. Loved all the others in the series also though ever since I was a kid.


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