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Offlinedarkhollow
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agar newbie - parafilm vs micro-pore tape vs -
    #24237380 - 04/12/17 12:14 PM (7 years, 9 months ago)

So I made my first 30 plates of agar MEA and all went well, except the use of micro-pore tape.  I found it to be quite tedious putting on, taking off and then re applying when using my plates. 

Is parafilm that much easier? It seems expensive, but I'm more interested in ease of use.  I see people on here posting about doing 100 plates a night... in my current configuration I would pull my hair out in no time. 

So my question is.. what is the easiest to use to seal your plates for storage prior to use and during colonization?

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Re: agar newbie - parafilm vs micro-pore tape vs - [Re: darkhollow]
    #24237392 - 04/12/17 12:25 PM (7 years, 9 months ago)

Following and in the same boat...though I did order two rolls of grafting parafilm. I could use it for grafting if it fails for the plates. Still, i'd like to see the responses.

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Re: agar newbie - parafilm vs micro-pore tape vs - [Re: darkhollow] * 1
    #24239829 - 04/13/17 09:29 AM (7 years, 9 months ago)

So it looks to me like this question has been answered heavily and my poor search skills failed me prior to posting.

General consensus is that parafilm is much easier and goes a very long way.  It's an expensive first purchase, but it lasts. 

Others mention that a low cost solution is saran wrap cut into 1 - 2" strips.

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Re: agar newbie - parafilm vs micro-pore tape vs - [Re: darkhollow] * 1
    #24239845 - 04/13/17 09:34 AM (7 years, 9 months ago)

Quote:

darkhollow said:
So it looks to me like this question has been answered heavily and my poor search skills failed me prior to posting.

General consensus is that parafilm is much easier and goes a very long way.  It's an expensive first purchase, but it lasts. 

Others mention that a low cost solution is saran wrap cut into 1 - 2" strips.



No.  Saran wrap won't allow for GE.  It's fine for storing them before use,  but once you use them,  you need breathable glad wrap (cut into 2" strips).


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Re: agar newbie - parafilm vs micro-pore tape vs - [Re: dhype773] * 1
    #24239976 - 04/13/17 10:19 AM (7 years, 9 months ago)

Saran or glad works just fine.
There's enough air in the plate to support all the growt even pin formation. Advice from experience people I've never seen someone using saran fail.

Don't use tape it leaves residue on your dishes

I prefer cling wrap to parafilm just for ease of use
Quote:

bodhisatta said:
Wrapping Petri Dishes
With glad/saran/cling wrap






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Re: agar newbie - parafilm vs micro-pore tape vs - [Re: bodhisatta]
    #24240088 - 04/13/17 10:59 AM (7 years, 9 months ago)

Quote:

bodhisatta said:
Saran or glad works just fine.
There's enough air in the plate to support all the growt even pin formation. Advice from experience people I've never seen someone using saran fail.

Don't use tape it leaves residue on your dishes

I prefer cling wrap to parafilm just for ease of use
Quote:

bodhisatta said:
Wrapping Petri Dishes
With glad/saran/cling wrap









I've never used saran because when I first started agar, I remember reading that it doesn't work.  Good to know,  I will give it a try tomorrow.  I plan on making 20 plates of PDA.  Definitely trust Bod OP, sorry for the bad info.


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Offlinedarkhollow
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Re: agar newbie - parafilm vs micro-pore tape vs - [Re: dhype773]
    #24240186 - 04/13/17 11:35 AM (7 years, 9 months ago)

Great to know.  Cling wrap for the win. 

Are people reusing the dishes?  Glass pyrex dishes are sexy, but damn pricey.  I haven't been throwing out dishes, but was wondering if people cleaned, sterilized and reused.

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Re: agar newbie - parafilm vs micro-pore tape vs - [Re: darkhollow]
    #24240213 - 04/13/17 11:43 AM (7 years, 9 months ago)

You can't reuse disposable dishes. I have pyrex dishes they blow dick so i use disposables exclusively. Reusing dishes is a pain in the dick and probably ends up being less eco friendly by the time you use water and soap/detergent then having to run a sterilization cycle to sterilize the dishes again.

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Offlinedarkhollow
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Re: agar newbie - parafilm vs micro-pore tape vs - [Re: bodhisatta]
    #24240480 - 04/13/17 01:26 PM (7 years, 9 months ago)

Thanks.  I figured as much, but you never know.

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OfflinePlantManBee
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Re: agar newbie - parafilm vs micro-pore tape vs - [Re: darkhollow]
    #24240546 - 04/13/17 01:55 PM (7 years, 9 months ago)

yeah, I used micropore tape once and that was a bitch cleaning them afterward. AFA glass vs. disposables, I used disposables and liked them but burned through them fast (due to user error). NOBODY likes to use glass, but I've opted for them anyway because ultimately they are less wasteful. They also vary A LOT in price and quality, and take some real care and planning to clean, dry, stack and wrap before PCing or autoclaving.

I have NOT found much info differentiating the Grafting Parafilm and the stuff meant for pertis.  Anyone using the grafting film? I do more plant work than fungus work so I will use it either way, but it would be good to hear.

Thanks y'all. :stoned:

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Re: agar newbie - parafilm vs micro-pore tape vs - [Re: PlantManBee]
    #24240682 - 04/13/17 02:58 PM (7 years, 9 months ago)

I wouldn't use micropore on plates.

Parafilm is pretty fast and easy, works really well but you have to cut the strips and peel which is kinda a PITA.

I use a combination of Parafilm and cling wrap, depending on how many plates I'm doing/how much time I have


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Offline99.99
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Re: agar newbie - parafilm vs micro-pore tape vs - [Re: mushpunx]
    #24240781 - 04/13/17 03:41 PM (7 years, 9 months ago)

Try these from big lots they're only $1.80 for three of them there safe for the pressure cooker as long as you do a Weep hole with micropore tape on top

[url=https://files.shroomery.org/files/17-008/806531385-image.jpg][/

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Re: agar newbie - parafilm vs micro-pore tape vs - [Re: 99.99]
    #24240800 - 04/13/17 03:46 PM (7 years, 9 months ago)

Quote:

99.99 said:
Try these from big lots they're only $1.80 for three of them there safe for the pressure cooker as long as you do a Weep hole with micropore tape on top

[url=https://files.shroomery.org/files/17-008/806531385-image.jpg][/





Those are pretty sweet, but check this out too search for microwave safe 8oz deli containers, they're PP5 and have clear tops. You can get packs of 100 for less than 10$


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Re: agar newbie - parafilm vs micro-pore tape vs - [Re: mushpunx]
    #24240817 - 04/13/17 03:54 PM (7 years, 9 months ago)

always used cling film. Only downside is cutting a roll can go wrong. I've found using a very sharp demi-chef cooking knife works really well, as long as I make a circular cut by turning the roll every time, instead of going straight down. That left me with cling film that stuck to the roll more than to the petris.

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Re: agar newbie - parafilm vs micro-pore tape vs - [Re: mushpunx]
    #24240830 - 04/13/17 03:58 PM (7 years, 9 months ago)

Packs of 100 for $10 give me a link all I could find was 40 packs for 17

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Re: agar newbie - parafilm vs micro-pore tape vs - [Re: 99.99]
    #24240864 - 04/13/17 04:12 PM (7 years, 9 months ago)

you know how much you would pay over here just to get a bottle tek suitable container??? I was in the hardware store yesterday, and the Systema brand (which they have everywhere nowadays here, pretty much the only brand with a screwlid) costs, for 1 (ONE!!!) approx. 500 ml container................................................................................................................................................................................................................................................................................................................................................................................................................................7,50 bucks! That's euro's, but fuck me, I could buy a week worth of ORGANIC vegetables for 3 of those fuckers. And that's for me, the misses AND the kid, and we eat lots of veggies.

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Re: agar newbie - parafilm vs micro-pore tape vs - [Re: Reblackah]
    #24240881 - 04/13/17 04:19 PM (7 years, 9 months ago)

Quote:

GoldenPreacher said:
as long as I make a circular cut by turning the roll every time, instead of going straight down. That left me with cling film that stuck to the roll more than to the petris.



Exactly. When it leaves a bur on the cardboard is the only time it's a pain in the ass. Breaks of halfway around the plate


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Re: agar newbie - parafilm vs micro-pore tape vs - [Re: Pipefitter537]
    #24240903 - 04/13/17 04:25 PM (7 years, 9 months ago)

Got me flaming..

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Re: agar newbie - parafilm vs micro-pore tape vs - [Re: Reblackah]
    #24241225 - 04/13/17 06:35 PM (7 years, 9 months ago)

Quote:

GoldenPreacher said:
you know how much you would pay over here just to get a bottle tek suitable container??? I was in the hardware store yesterday, and the Systema brand (which they have everywhere nowadays here, pretty much the only brand with a screwlid) costs, for 1 (ONE!!!) approx. 500 ml container................................................................................................................................................................................................................................................................................................................................................................................................................................7,50 bucks! That's euro's, but fuck me, I could buy a week worth of ORGANIC vegetables for 3 of those fuckers. And that's for me, the misses AND the kid, and we eat lots of veggies.




You have EBAY im sure
Look at this on eBay:

https://www.ebay.com/itm/162314583549

40 Microwavable Freezer and Dishwasher Safe 8 oz. Round Deli Food Container NEW

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Offline99.99
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Re: agar newbie - parafilm vs micro-pore tape vs - [Re: 99.99]
    #24241258 - 04/13/17 06:43 PM (7 years, 9 months ago)


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Re: agar newbie - parafilm vs micro-pore tape vs - [Re: 99.99]
    #24241295 - 04/13/17 06:57 PM (7 years, 9 months ago)

Quote:

99.99 said:
Even better 240 pcs
18.99

http://www.webstaurantstore.com/choicehd-8-oz-microwavable-translucent-plastic-deli-container-and-lid-combo-pack-240-case/128HD8COMBO.html?utm_source=Google&utm_medium=cpc&utm_campaign=GoogleShopping&gclid=CLeEj4LbotMCFZaFswod9joPhQ




Yea see? I haven't tried these yet but I used to ship them out, I worked in a warehouse that sold restaurant supplies. They're shallower than Glad Mini Rounds too which is nice, I think the lids will probably hold tight (my mini rounds fall apart sometimes if I knock em around)
And they're wide too! Can grow cultures out big!


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Re: agar newbie - parafilm vs micro-pore tape vs - [Re: mushpunx]
    #24241340 - 04/13/17 07:11 PM (7 years, 9 months ago)

I find parafilm easy to use and I figure the 125 ft. roll of parafilm laboratory tape I got would easily suffice for over a thousand plates.

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Re: agar newbie - parafilm vs micro-pore tape vs - [Re: zajcob]
    #24241381 - 04/13/17 07:23 PM (7 years, 9 months ago)

Quote:

zajcob said:
I find parafilm easy to use and I figure the 125 ft. roll of parafilm laboratory tape I got would easily suffice for over a thousand plates.




it will cover MANY, but nowhere near a thousand.

circumference is pi x diameter
diameter of a typical petri is 90 mm
90 mm is 3.54331 inches
3.54331 x 3.14= 11.1259934 inches

11 inches rounded up to a foot. I wouldn't count on more than 130. That is a fuck load of agar

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Re: agar newbie - parafilm vs micro-pore tape vs - [Re: PlantManBee]
    #24241458 - 04/13/17 07:49 PM (7 years, 9 months ago)

The calculation I used is much simpler.  I simply cut a strip that is not too much wider than an inch.  That suffices to wrap a single plate around its circumference, stretchy as parafilm is.  So its basically 12 inches x 125 feet. Since I said it's maybe a little wider than an inch, I can just say about 10 x 125, which equals 1250, and thus easily over a thousand plates.  If you you count the times I screw up with the parafilm, it's closer to a thousand.  But if you are using two strips per plate, which will often be the case, one for when it is poured, and then one for after a transfer, I guess it would surely be closer to 500!  Still pretty good though, inasmuch as my estimates are valid.

Edited by zajcob (04/13/17 07:50 PM)

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Re: agar newbie - parafilm vs micro-pore tape vs - [Re: zajcob]
    #24242376 - 04/14/17 02:17 AM (7 years, 9 months ago)

wow... THANKS!!!  stupid that i didn't think of that :p The ones you sent didn't have screwtop lids though, but i'll do a search next time i'm buying new supplies.

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Re: agar newbie - parafilm vs micro-pore tape vs - [Re: Reblackah] * 1
    #24242526 - 04/14/17 04:10 AM (7 years, 9 months ago)

hey buddy, i am probably that asshole you read about doing 100-200 plates a day :lol:

i fuckin looooooooooove parafilm, woulndt dare do agar work without it. its one of those things that you learn to depend on a LOT in the lab, its so damned useful

i love all lab equipment, its always good to look at how the pros do whatever it is you are trying to do, and then learn from their techniques, etc

here is the video i first learned to wrap parafilm correctly from (was wasting soooooo much before this)



i will be making an agar tips thread or journal soon, will include lots of footage of some time saving tips and tricks for working with LOTS of plates, mainly just stuff i picked up from lab manuals and experience (and bodhi)

bods right about the air in the plate. parafilm is ideal and does provide GE, but for what most people are doing, it doesnt really matter since there is plenty of air to support the culture in the plate

DONT DONT DONT use micropore, it makes a mess and attracts contams, use parafilm or saran wrap

my workflow: i work in groups of 60, wrap each plate in parafilm as above, and then label and place each plate in an individual sandwich ziploc bag, so i can handle it freely without worry

i think a 1000+ sounds right for a 125' x 4" roll, but i usually get the 250' rolls. i always cut a 4 ft length , then pull out 4 ft more and clip em on top of each other, then use a retractable scalpel to cut 3/4" strips (2 at a time) and keep this stack handy


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Re: agar newbie - parafilm vs micro-pore tape vs - [Re: mushpunx]
    #24242606 - 04/14/17 05:48 AM (7 years, 9 months ago)

Quote:

GoldenPreacher said:
always used cling film. Only downside is cutting a roll can go wrong. I've found using a very sharp demi-chef cooking knife works really well, as long as I make a circular cut by turning the roll every time, instead of going straight down. That left me with cling film that stuck to the roll more than to the petris.



Utility knife works great as well
Quote:

mushpunx said:
Quote:

99.99 said:
Even better 240 pcs
18.99

http://www.webstaurantstore.com/choicehd-8-oz-microwavable-translucent-plastic-deli-container-and-lid-combo-pack-240-case/128HD8COMBO.html?utm_source=Google&utm_medium=cpc&utm_campaign=GoogleShopping&gclid=CLeEj4LbotMCFZaFswod9joPhQ




Yea see? I haven't tried these yet but I used to ship them out, I worked in a warehouse that sold restaurant supplies. They're shallower than Glad Mini Rounds too which is nice, I think the lids will probably hold tight (my mini rounds fall apart sometimes if I knock em around)
And they're wide too! Can grow cultures out big!



Just got a few different brands of these types of containers and pc's some tonight.

One of the brands that I got had less see through of plastic as the others so I ended not even trying those.

Also the tops fit very solid I just hope that a few pc cycles will actually loosen them a bit.

I really like the fact that they really solid around the seal.

Will update asap...


--------------------

Edited by tombosley8 (04/14/17 01:13 PM)

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Re: agar newbie - parafilm vs micro-pore tape vs - [Re: tombosley8]
    #24242966 - 04/14/17 08:53 AM (7 years, 9 months ago)

Ah... 4 inches wide makes a hell of a difference. My bad.

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Re: agar newbie - parafilm vs micro-pore tape vs - [Re: PlantManBee]
    #24261212 - 04/21/17 07:06 AM (7 years, 8 months ago)

I bought some parafilm and I have to say it's a dream to work with.  I bought the 4" and just cut one strip for each plate.  So fast and easy to work with.

And I can double use it as grafting tape.

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Re: agar newbie - parafilm vs micro-pore tape vs - [Re: tombosley8]
    #24264594 - 04/22/17 05:34 PM (7 years, 8 months ago)

Quote:

tombosley8 said:
Quote:

GoldenPreacher said:
always used cling film. Only downside is cutting a roll can go wrong. I've found using a very sharp demi-chef cooking knife works really well, as long as I make a circular cut by turning the roll every time, instead of going straight down. That left me with cling film that stuck to the roll more than to the petris.



Utility knife works great as well
Quote:

mushpunx said:
Quote:

99.99 said:
Even better 240 pcs
18.99

http://www.webstaurantstore.com/choicehd-8-oz-microwavable-translucent-plastic-deli-container-and-lid-combo-pack-240-case/128HD8COMBO.html?utm_source=Google&utm_medium=cpc&utm_campaign=GoogleShopping&gclid=CLeEj4LbotMCFZaFswod9joPhQ




Yea see? I haven't tried these yet but I used to ship them out, I worked in a warehouse that sold restaurant supplies. They're shallower than Glad Mini Rounds too which is nice, I think the lids will probably hold tight (my mini rounds fall apart sometimes if I knock em around)
And they're wide too! Can grow cultures out big!



Just got a few different brands of these types of containers and pc's some tonight.

One of the brands that I got had less see through of plastic as the others so I ended not even trying those.

Also the tops fit very solid I just hope that a few pc cycles will actually loosen them a bit.

I really like the fact that they really solid around the seal.

Will update asap...




Well i bought a case of 240 pcs  that says microwave safe with lids their fucking pieces of shit every single one melted in my PC

I'm going back to my  containers from big lots 1.80 for three of them

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Re: agar newbie - parafilm vs micro-pore tape vs - [Re: 99.99]
    #24264705 - 04/22/17 06:15 PM (7 years, 8 months ago)

I just use black insulation pvc tape. Works fine for me


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Re: agar newbie - parafilm vs micro-pore tape vs - [Re: 99.99] * 1
    #24264825 - 04/22/17 07:04 PM (7 years, 8 months ago)

Quote:

99.99 said:
Quote:

tombosley8 said:
Quote:

GoldenPreacher said:
always used cling film. Only downside is cutting a roll can go wrong. I've found using a very sharp demi-chef cooking knife works really well, as long as I make a circular cut by turning the roll every time, instead of going straight down. That left me with cling film that stuck to the roll more than to the petris.



Utility knife works great as well
Quote:

mushpunx said:
Quote:

99.99 said:
Even better 240 pcs
18.99

http://www.webstaurantstore.com/choicehd-8-oz-microwavable-translucent-plastic-deli-container-and-lid-combo-pack-240-case/128HD8COMBO.html?utm_source=Google&utm_medium=cpc&utm_campaign=GoogleShopping&gclid=CLeEj4LbotMCFZaFswod9joPhQ




Yea see? I haven't tried these yet but I used to ship them out, I worked in a warehouse that sold restaurant supplies. They're shallower than Glad Mini Rounds too which is nice, I think the lids will probably hold tight (my mini rounds fall apart sometimes if I knock em around)
And they're wide too! Can grow cultures out big!



Just got a few different brands of these types of containers and pc's some tonight.

One of the brands that I got had less see through of plastic as the others so I ended not even trying those.

Also the tops fit very solid I just hope that a few pc cycles will actually loosen them a bit.

I really like the fact that they really solid around the seal.

Will update asap...




Well i bought a case of 240 pcs  that says microwave safe with lids their fucking pieces of shit every single one melted in my PC

I'm going back to my  containers from big lots 1.80 for three of them




Were they PP5 recycling code though? That's what you gotta check for


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Offlinetombosley8
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Registered: 10/14/13
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Re: agar newbie - parafilm vs micro-pore tape vs - [Re: mushpunx]
    #24265230 - 04/22/17 11:01 PM (7 years, 8 months ago)

Yeah I bought a few different kinds on amazon and finally only ended up trying two of them luckily as I just looked and all but one lid had holes melted in them and the one that didn't warped so bad a little touch smashed right through it. bummer

The containers seemed to hold up very well(other than the lid) so I was almost debating putting silicon between two lids and sandwiching them together although obviously that would take away being able to see through the lid. I just like the containers even if I had to see through the sides. probobly would still melt idk?


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Edited by tombosley8 (04/22/17 11:02 PM)

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InvisibleBLUET00TH

Registered: 04/24/17
Posts: 2
Re: agar newbie - parafilm vs micro-pore tape vs - [Re: 99.99]
    #24269587 - 04/24/17 10:52 PM (7 years, 8 months ago)


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Offline99.99
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Re: agar newbie - parafilm vs micro-pore tape vs - [Re: BLUET00TH]
    #24269595 - 04/24/17 10:55 PM (7 years, 8 months ago)

Quote:

BLUET00TH said:
Quote:

99.99 said:
Even better 240 pcs
18.99

http://www.webstaurantstore.com/choicehd-8-oz-microwavable-translucent-plastic-deli-container-and-lid-combo-pack-240-case/128HD8COMBO.html?utm_source=Google&utm_medium=cpc&utm_campaign=GoogleShopping&gclid=CLeEj4LbotMCFZaFswod9joPhQ





The lids are a different plastic than the containers and are not PC safe. :sad:




Yeah i found that out after my first batch in the pc ...FUCK

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InvisibleReblackah
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Registered: 01/07/15
Posts: 13,770
Re: agar newbie - parafilm vs micro-pore tape vs - [Re: 99.99]
    #24270788 - 04/25/17 02:06 PM (7 years, 8 months ago)

I'm saving up on PP5 airtight yoghurt containers, but at 2 a week, things are going slow... Ah well, at least I'm saving on the plastic waste AND I'm not spending extra on containers...

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Invisiblejohn_conner

Registered: 02/21/17
Posts: 243
Re: agar newbie - parafilm vs micro-pore tape vs - [Re: bodhisatta]
    #24271520 - 04/25/17 07:21 PM (7 years, 8 months ago)

Thank you for this!

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