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OfflineThufir_Hawat
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First Grow Log: Golden Teacher on BRF * 2
    #24214280 - 04/02/17 10:26 PM (7 years, 9 months ago)

EDIT: Note for any future readers- I seem to have gotten some bunk spore syringes, only 1/8 jars colonized, and further attempts to isolate on agar also failed. HOWEVER, the fact that there were no fruits from the one jar that did colonize is entirely MY fault for not following the TEK. Follow the instructions, yo.


Lab Monitor

For my first grow I am following EvilMushroom666's Take on BRF Cakes, exceptions as noted. I read quite a few of the available teks and think I have the principles down, but please let me know what I'm doing wrong (there have to be a few things).


4/1/17: Mixing the substrate, 2:1:1 mix ratio


Prepped jars
Wide mouth 1/2 pint low jars, 4 hanging nail holes in the lids.


PC setup, 15psi for 60 minutes


4/2/17: Inoculation
My SAB construction is a little rough (drilled a series of small holes to cut the armholes out), but I think it functions as intended. Closed the window and door of the room for an hour before starting, showered, nitrile gloves, face mask, iso sterilized everything.
Flame sterilized (butane torch) outside SAB between each jar, 1cc per jar.

Currently resting on a shelf, waiting for something to test out my new lens extension tube on.


4/3/17: Due diligence update - no visible change
4/4/17: No visible change

Edited by Thufir_Hawat (05/10/17 07:51 PM)

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InvisiblemushboyMDiscord
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Re: First Grow Log: Golden Teacher on BRF [Re: Thufir_Hawat]
    #24214286 - 04/02/17 10:34 PM (7 years, 9 months ago)

off to a great start.:thumbup:

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OfflineBlueDisa
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Re: First Grow Log: Golden Teacher on BRF [Re: mushboy]
    #24214510 - 04/03/17 03:20 AM (7 years, 9 months ago)

Looks good, keep us updated. :smile:

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OfflineThufir_Hawat
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Re: First Grow Log: Golden Teacher on BRF [Re: BlueDisa] * 1
    #24222032 - 04/06/17 02:19 AM (7 years, 9 months ago)

First growth! Only two noticeable spots on a single jar out of four, but still!


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OfflineFungi2TripWit
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Re: First Grow Log: Golden Teacher on BRF [Re: Thufir_Hawat]
    #24222102 - 04/06/17 04:10 AM (7 years, 9 months ago)

Looks kind of fine haired and wispy like cobweb.  The fact that it looks like 2 separate inoculation points close to each other make me think it's not myc either.  Then again, I'm not a professional, just read a shit ton on this site.  Time will truly tell if they are gonna make it.  Wish you luck man.


--------------------
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InvisibleCaptainRedbeard
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Re: First Grow Log: Golden Teacher on BRF [Re: Fungi2TripWit]
    #24222133 - 04/06/17 05:03 AM (7 years, 9 months ago)

Those are some nice high-res pics

That looks like mycelium to me - early stages

Great job :crazy2:

Keep us posted :thumbup:


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Offlinemushpunx
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Re: First Grow Log: Golden Teacher on BRF [Re: CaptainRedbeard]
    #24222143 - 04/06/17 05:09 AM (7 years, 9 months ago)

Yea its germination most likely :thumbup: you did leave the foil off after inoculation?


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Edited by mushpunx (04/06/17 05:11 AM)

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OfflineThufir_Hawat
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Re: First Grow Log: Golden Teacher on BRF [Re: mushpunx]
    #24222263 - 04/06/17 07:41 AM (7 years, 9 months ago)

That's right, foil off in SAB before inoculation and that's all she wrote.

No scale to compare to in the pics, but the site in the second pic was roughly 2mm wide.

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OfflineMyco Mako
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Re: First Grow Log: Golden Teacher on BRF [Re: Thufir_Hawat]
    #24222306 - 04/06/17 08:06 AM (7 years, 9 months ago)

Colonization is starting.  Just remember not to tilt or agitate your jars too much and should start seeing that expand.  Don't want to disturb your layer at the top to let the baddies in...  Nice!

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OfflineLogicaL ChaosM
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Re: First Grow Log: Golden Teacher on BRF [Re: Thufir_Hawat]
    #24222309 - 04/06/17 08:08 AM (7 years, 9 months ago)

Great photos man.

I like your kitty kat :grin:


--------------------
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OfflineThufir_Hawat
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Re: First Grow Log: Golden Teacher on BRF [Re: LogicaL Chaos]
    #24224065 - 04/06/17 09:40 PM (7 years, 9 months ago)

Still no visible activity in the other 3 jars, progress on the precocious one:

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Offlinelovelaughlibs
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Re: First Grow Log: Golden Teacher on BRF [Re: Thufir_Hawat]
    #24224625 - 04/07/17 05:02 AM (7 years, 9 months ago)

Cool macro shots :grin:


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InvisibleZiran
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Re: First Grow Log: Golden Teacher on BRF [Re: Thufir_Hawat]
    #24224702 - 04/07/17 06:03 AM (7 years, 9 months ago)

Quote:

Thufir_Hawat said:
Still no visible activity in the other 3 jars, progress on the precocious one:





good stuff :thumbup:


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InvisibleCaptainRedbeard
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Re: First Grow Log: Golden Teacher on BRF [Re: Ziran]
    #24224952 - 04/07/17 08:26 AM (7 years, 9 months ago)

Love seeing mycelium progress :crazy2:

Can't wait to see the shroomies form, down the line


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take it easy dude, but - take it!

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OfflineThufir_Hawat
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Re: First Grow Log: Golden Teacher on BRF [Re: CaptainRedbeard]
    #24226907 - 04/08/17 01:23 AM (7 years, 9 months ago)

Starting to think I didn't shake the syringe enough before the other 3 jars, but a new site appeared on the one that did get some spores:


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Offlinelovelaughlibs
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Re: First Grow Log: Golden Teacher on BRF [Re: Thufir_Hawat]
    #24227329 - 04/08/17 09:18 AM (7 years, 9 months ago)

Can take up to 10 days-2 weeks sometimes for germination, be patient man :smile:

Great growth so far!


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OfflineMyco Mako
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Re: First Grow Log: Golden Teacher on BRF [Re: Thufir_Hawat]
    #24228443 - 04/08/17 05:54 PM (7 years, 9 months ago)

Quote:

Thufir_Hawat said:
Starting to think I didn't shake the syringe enough before the other 3 jars, but a new site appeared on the one that did get some spores:






1 out of 4 holes is all it takes.  Not ideal, but it will get there.  I sometimes think my first jar where I've flame sterilized the needle ends up short on spores.  Always wondered if it was the flame sterilization, not shaking enough, etc.  Nice growth thus far!


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OfflineThufir_Hawat
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Re: First Grow Log: Golden Teacher on BRF [Re: Myco Mako]
    #24229101 - 04/08/17 11:15 PM (7 years, 9 months ago)

I didn't plan on posting every day, but I really like the pictures I got today :smile:



edit: 4/9/2017

Edited by Thufir_Hawat (04/10/17 12:09 AM)

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OfflineThufir_Hawat
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Re: First Grow Log: Golden Teacher on BRF [Re: Thufir_Hawat]
    #24238874 - 04/12/17 10:06 PM (7 years, 9 months ago)

4/12/2017

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Invisiblevan hattonFacebookDiscord
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Re: First Grow Log: Golden Teacher on BRF [Re: Thufir_Hawat]
    #24238895 - 04/12/17 10:13 PM (7 years, 9 months ago)

iso sterilized everything.


In your first post do you use Iso on the inside of your sab?

Also ISO is a sanitizer not sterilizer but it's all good :thumbup:

Nice job so far cute cat and good pics :rockon:


--------------------
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OfflineThufir_Hawat
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Re: First Grow Log: Golden Teacher on BRF [Re: van hatton]
    #24238969 - 04/12/17 10:57 PM (7 years, 9 months ago)

Thanks, and yeah I wiped down the inside with 70% on a paper towel.

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OfflineThufir_Hawat
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Re: First Grow Log: Golden Teacher on BRF [Re: Thufir_Hawat]
    #24239208 - 04/13/17 01:33 AM (7 years, 9 months ago)

So, I inoculated a thing...


Decided to have some fun and made up four tyvek/silicone lids, two each of single injection point and four point.


Each set of two lids (one of each type) is over two half pint jars of either:
Coffee grounds (used), mixed with mango, orange, and banana peels, with a bit of old vaped weed:

OR
50% the above to 50% vermiculite:


I know these are not ideal or recommended things to fill jars with, but I'm just doing this for fun so why not? 
:lahey:

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OfflineThufir_Hawat
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Re: First Grow Log: Golden Teacher on BRF [Re: Thufir_Hawat]
    #24248477 - 04/16/17 08:52 AM (7 years, 8 months ago)

Well, the one jar is still colonizing nicely, but nothing at all in any of the other 7 jars...

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OfflineThufir_Hawat
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Re: First Grow Log: Golden Teacher on BRF [Re: Thufir_Hawat]
    #24251319 - 04/17/17 10:24 AM (7 years, 8 months ago)

Any ideas why nothing apart from the one jar has germinated?

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Invisiblemynakedrat
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Re: First Grow Log: Golden Teacher on BRF [Re: Thufir_Hawat]
    #24251479 - 04/17/17 11:39 AM (7 years, 8 months ago)

What?
Mango and weed?

No grains?

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OfflineThufir_Hawat
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Re: First Grow Log: Golden Teacher on BRF [Re: mynakedrat]
    #24252279 - 04/17/17 05:59 PM (7 years, 8 months ago)

Mostly coffee grounds, the first set of jars was brown rice flour and verm though, and only one of those germinated.

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OfflineHon
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Re: First Grow Log: Golden Teacher on BRF [Re: Thufir_Hawat]
    #24252289 - 04/17/17 06:02 PM (7 years, 8 months ago)

nothin like a nice golden skeeter

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Invisiblemynakedrat
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Re: First Grow Log: Golden Teacher on BRF [Re: Hon]
    #24252491 - 04/17/17 07:02 PM (7 years, 8 months ago)

Aaah skeet skeet skeet

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OfflineThufir_Hawat
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Re: First Grow Log: Golden Teacher on BRF [Re: mynakedrat]
    #24268265 - 04/24/17 11:04 AM (7 years, 8 months ago)

Nothing from the other jars, but this one is finally almost just not quite ready to pop out:

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OfflineThufir_Hawat
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Re: First Grow Log: Golden Teacher on BRF [Re: Thufir_Hawat]
    #24271956 - 04/25/17 11:14 PM (7 years, 8 months ago)

Well, because I am a glutton for punishment, instead of birthing that cake I mixed it up with the others (just the other 3 BRF jars, not the coffee) which did not colonize (minus their casing layers) and stuck it all in a big container.

All done in SAB after bleach misting and waiting, alcohol sanitation of everything before and after putting into the SAB



I'm not in a rush, so I figured why not have some fun and maybe get some more out of that (very nicely colonized) cake. It was a really interesting experience taking it out of the jar and feeling how dense it was, took a surprising amount of strength to tear apart, and the smell was... It just kind of smelled like, life? That sounds really cheesy but it was all I could think of at the time.

I also just started my first agar minirounds with drops from a Pink Buffalo syringe. Hopefully those start growing soon.

Edited by Thufir_Hawat (04/25/17 11:15 PM)

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OfflineFungi2TripWit
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Re: First Grow Log: Golden Teacher on BRF [Re: Thufir_Hawat]
    #24280214 - 04/28/17 11:25 PM (7 years, 8 months ago)

Man, you are doing an awful lot of experimenting for your first grow.  I'd recommend following a tek exactly as it is written.  Then you can get to know the mushrooms and their growing process better.  No use in experimenting if you don't know what you are doing.  ie.  mixing a good cake with ones that were most likely  contaminated or trying to grow mushrooms with fruit instead of grain.  Sounds like you are just having fun but if you ever want to get serious, then follow directions and observe for a while and you can do experiments based on your observations. I am also on my first grow.  Someone on here told me to just follow the damn tek for your first grow and here is what happens when you just follow the tek.

From montub #1





First flush



Second flush



jars just shaken so they will be ready to spawn in 3-5 days and be ready to spawn tub #1 again.



Tub #1, probably will be a tiny flush but the jars will be ready soon.




Tub #2, spawned 8 days ago.




Tray from sgfc spawned 8 days ago.


I have mushrooms at all stages mostly because I've followed the tek, but secondary I read and calculated the times in each stage to pretty much have a flush every week.  My point is that you can fuck around and experiment or you can grow mushrooms.  The information to do it is here.  When someone tells you to do something on here, do it, don't try and be creative and cool.. 





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Edited by Fungi2TripWit (04/29/17 12:23 AM)

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OfflineThufir_Hawat
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Re: First Grow Log: Golden Teacher on BRF [Re: Fungi2TripWit]
    #24280276 - 04/28/17 11:58 PM (7 years, 8 months ago)

You're right, and that was the plan with the PF jars until I decided to do that for reasons still beyond my own comprehension. Too much free time lately.  :bongload:

Hopefully some of the PB spores I put on agar a few days ago take off so I can start fresh and really do it right (Agar to grain to mono).

edit: Since doing that transfer/combination I'm reasonably sure there was Bacillus growing in the colonized jar, from the fermenting apple smell.

Edited by Thufir_Hawat (04/28/17 11:59 PM)

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OfflineThufir_Hawat
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Re: First Grow Log: Golden Teacher on BRF [Re: Thufir_Hawat]
    #24280291 - 04/29/17 12:03 AM (7 years, 8 months ago)

That... or V-Tek bottle grow... Haven't decided.

Also read through the sponsor forum and it seems the viability of my syringes might not be an isolated incident. :undecided:

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OfflineFungi2TripWit
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Re: First Grow Log: Golden Teacher on BRF [Re: Thufir_Hawat]
    #24280337 - 04/29/17 12:38 AM (7 years, 8 months ago)

Sorry, I was editing my post when you responded.  Your best bet would have been to do the v-tek if you don't have a sgfc.  Shredded it with a cheese grater and mixed with a substrate in a tray if you do have a sgfc.  I'm not really familiar with the v-tek but you could have done something similar to a tray inside a bottle I guess.


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OfflineThufir_Hawat
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Re: First Grow Log: Golden Teacher on BRF [Re: Fungi2TripWit]
    #24280339 - 04/29/17 12:43 AM (7 years, 8 months ago)

I did make an SGFC, it currently has my slab of oyster mycellium in it haha.

e: I am pretty sure the yield from a single small tub would be more than enough shrooms to last me a while haha, I'm not looking to have a bunch around.

Edited by Thufir_Hawat (04/29/17 12:45 AM)

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OfflineFungi2TripWit
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Re: First Grow Log: Golden Teacher on BRF [Re: Thufir_Hawat]
    #24280351 - 04/29/17 12:49 AM (7 years, 8 months ago)

You could have birthed the cake and put it in with the oyster.  At least you would have made an attempt at that stage and got some experience.


--------------------
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OfflineThufir_Hawat
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Re: First Grow Log: Golden Teacher on BRF [Re: Fungi2TripWit]
    #24280352 - 04/29/17 12:51 AM (7 years, 8 months ago)

Right, that's what my plan was, I just had a goofy whim and went with it when I shouldn't have. Them's the breaks.

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