Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Left Coast Kratom Buy Kratom Extract   Myyco.com Isolated Cubensis Liquid Culture For Sale   MagicBag.co All-In-One Bags That Don't Suck   North Spore North Spore Mushroom Grow Kits & Cultivation Supplies   Mushroom-Hut Substrate Bags   Original Sensible Seeds Bulk Cannabis Seeds

Jump to first unread post Pages: < First | < Back | 255 | 256 | 257 | 258 | 259 | 260 | 261 | 262 | 263 | 264 | 265 | 266 | 267 | 268 | 269 | 270 | 271 | 272 | 273 | 274 | 275 | Next > | Last >
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
InvisibleTheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
Re: Pasty Agar Tek [Re: tryptkaloids]
    #24207931 - 03/31/17 11:29 AM (6 years, 10 months ago)

So I have had a few "wedges" that after transfer, well part of them seem to almost melt into the new agar plate, takes a couple days. Is this just the mycelium digesting part of the wedge?


--------------------
JOIN THE POW WOW


Extras: Filter Print Post Top
Offlinetryptkaloids
Learner
I'm a teapot


Registered: 02/08/15
Posts: 12,649
Loc: Exact Center
Last seen: 3 days, 21 hours
Trusted Cultivator
Re: Pasty Agar Tek [Re: stareatclouds]
    #24207947 - 03/31/17 11:39 AM (6 years, 10 months ago)

Quote:

stareatclouds said:
If I'm seeing little satellite colonies a full week after myc transfers, what's the likely culprit? They're happening in both Pasty Plate's (with parafilm around them) and glass 1/2 pints with SFDs/surgical tape.

The actual myc growth looks great, but a week after initial transfer, tiny satellites pop up kinda far away from the myc growth.

This hasn't happened to my petri dishes.



if it still looks like cube myc then it probably is. the reason it takes longer is because the growth is microscopic on the smaller inoculation points


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


Extras: Filter Print Post Top
Offlineshumer44
 User Gallery


Registered: 11/15/16
Posts: 255
Last seen: 6 years, 1 day
Re: Pasty Agar Tek [Re: tryptkaloids]
    #24208323 - 03/31/17 02:00 PM (6 years, 10 months ago)

Ok I bought potato flakes (unflavored).

1. Which is better, inject spores into all the agar plates or Inject only on one agar plate, and if successful, transfer that inoculated agar into other plates.

2. After cooking the agar, should I wait for it to cool down before transferring it to the mini rounds, or little warm is fine?

3. 2.5g of agar powder can make how many plates?


Edited by shumer44 (03/31/17 03:05 PM)


Extras: Filter Print Post Top
Offlineshumer44
 User Gallery


Registered: 11/15/16
Posts: 255
Last seen: 6 years, 1 day
Re: Pasty Agar Tek [Re: shumer44]
    #24208528 - 03/31/17 03:17 PM (6 years, 10 months ago)

Does the final result have to be liquid or little solid?


Extras: Filter Print Post Top
OfflineKenetic
Nam Sayin
 User Gallery


Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land Flag
Last seen: 5 years, 4 months
Re: Pasty Agar Tek [Re: TheMadHatter420]
    #24208536 - 03/31/17 03:20 PM (6 years, 10 months ago)

Quote:

TheMadHatter420 said:
So I have had a few "wedges" that after transfer, well part of them seem to almost melt into the new agar plate, takes a couple days. Is this just the mycelium digesting part of the wedge?




Not relevant, whatever it is (that means it doesn't matter)


--------------------
Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet


Extras: Filter Print Post Top
InvisibleMoabfighter
Tam Fighter
 User Gallery

Registered: 12/13/15
Posts: 2,710
Re: Pasty Agar Tek [Re: Kenetic]
    #24208572 - 03/31/17 03:39 PM (6 years, 10 months ago)

If you use 2.5g of agar to half a cup of water, it's gonna be harder than a rock.


Try 1.9 agar, 1TSP potato flakes, and the smallest drop of honey you can get.

Please pasty, not stepping on your toes. Wouldn't have plates without this Tek. But the recipe I posted above makes nice soft plates, and encourages rhizomorphic growth due to low nutes.

Maybe not the best recipe for spores, but for transfers, that's what I do. This recipe also breaks up very easy on liquid innoculant


--------------------
KSSS And PE WBS.
 


Extras: Filter Print Post Top
OfflineKenetic
Nam Sayin
 User Gallery


Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land Flag
Last seen: 5 years, 4 months
Re: Pasty Agar Tek [Re: Moabfighter]
    #24208594 - 03/31/17 03:45 PM (6 years, 10 months ago)

when you gonna change your av yo


--------------------
Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet


Extras: Filter Print Post Top
Offlineshumer44
 User Gallery


Registered: 11/15/16
Posts: 255
Last seen: 6 years, 1 day
Re: Pasty Agar Tek [Re: Kenetic]
    #24208603 - 03/31/17 03:49 PM (6 years, 10 months ago)

I think i added too much agar and flakes, and 2 drops of honey. i have 8 glad rounds prepared already.

Waiting for them to cool down then i will PC them. If i should redo everything, pls tell.


Extras: Filter Print Post Top
Offlineshumer44
 User Gallery


Registered: 11/15/16
Posts: 255
Last seen: 6 years, 1 day
Re: Pasty Agar Tek [Re: shumer44]
    #24208624 - 03/31/17 03:58 PM (6 years, 10 months ago)

I placed warm agar liquid into the glad rounds and closed it, before PCing, condensation already build up inside. Is this safe, should i just PC?


Extras: Filter Print Post Top
InvisibleComebackKid
Multispore Enthusiast
Male User Gallery


Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
Trusted Cultivator
Re: Pasty Agar Tek [Re: shumer44]
    #24209174 - 03/31/17 07:42 PM (6 years, 10 months ago)

Dude just go for it :lol: I'm sure you'll do just fine. Condensation is expected. If you follow the tek and somehow it doesn't work then go back and follow it again till it does work. Its a proven method...
Knock up as many plates as you want, you can always make more :shrug:


--------------------
:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself


Extras: Filter Print Post Top
Offlineshumer44
 User Gallery


Registered: 11/15/16
Posts: 255
Last seen: 6 years, 1 day
Re: Pasty Agar Tek [Re: ComebackKid]
    #24209284 - 03/31/17 08:29 PM (6 years, 10 months ago)

I actually added more than needed (the ingredients). PC is done. Will inoculate tomorrow.


Extras: Filter Print Post Top
InvisibleJosex
#cheat_code
 User Gallery

Registered: 11/13/15
Posts: 8,995
Loc: Flag
Trusted Cultivator
Re: Pasty Agar Tek [Re: shumer44]
    #24209303 - 03/31/17 08:38 PM (6 years, 10 months ago)



With those eyes nobody will resist answering at least 5% of your questions.

Now we got the prep down dude :super:
We better buckle down with the sterile procedure, tomorrow...


Extras: Filter Print Post Top
Offlinetryptkaloids
Learner
I'm a teapot


Registered: 02/08/15
Posts: 12,649
Loc: Exact Center
Last seen: 3 days, 21 hours
Trusted Cultivator
Re: Pasty Agar Tek [Re: Josex]
    #24210446 - 04/01/17 10:13 AM (6 years, 10 months ago)

based on your questions I would suggest some more research.. the links in my signature are all cultivation/agar related and should help you out.


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


Extras: Filter Print Post Top
Offlineshumer44
 User Gallery


Registered: 11/15/16
Posts: 255
Last seen: 6 years, 1 day
Re: Pasty Agar Tek [Re: tryptkaloids]
    #24211470 - 04/01/17 05:41 PM (6 years, 10 months ago)

After PCing yesterday, today I opened it and inoculated.

I drilled the holes on side, so there was only tyvek (which got wet). Before inoculation, I added another layer of tyvek.

Top outer parts of glad mini rounds had water, I just shacked them and it was gone.

But inside of the glad mini rounds also had condensation build up on top parts, I removed it by shacking just second before inoculation.

And.. right now all the plates are inside SAB, I will remove them after couple of hours and place them inside cabinet.

Don't know how this will go. :frown:


Edited by shumer44 (04/01/17 05:42 PM)


Extras: Filter Print Post Top
Offlinetryptkaloids
Learner
I'm a teapot


Registered: 02/08/15
Posts: 12,649
Loc: Exact Center
Last seen: 3 days, 21 hours
Trusted Cultivator
Re: Pasty Agar Tek [Re: shumer44]
    #24211571 - 04/01/17 06:21 PM (6 years, 10 months ago)

stop looking down on yourself. you'll do fine, start a grow log in your journal so you can freak out there. let's not derail this thread with every move you make


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


Extras: Filter Print Post Top
Offlineshumer44
 User Gallery


Registered: 11/15/16
Posts: 255
Last seen: 6 years, 1 day
Re: Pasty Agar Tek [Re: tryptkaloids]
    #24211596 - 04/01/17 06:35 PM (6 years, 10 months ago)

Quote:

tryptkaloids said:
stop looking down on yourself. you'll do fine, start a grow log in your journal so you can freak out there. let's not derail this thread with every move you make




yes i will.


Edited by shumer44 (04/01/17 06:36 PM)


Extras: Filter Print Post Top
OfflineD.little91
Windowlicker
Male User Gallery

Registered: 01/13/15
Posts: 260
Loc: Western PA
Last seen: 3 years, 5 months
Re: Pasty Agar Tek [Re: shumer44]
    #24218422 - 04/04/17 04:41 PM (6 years, 10 months ago)

Hello everyone. So I followed the steps and here I am about a week later and it appears there is some milky colored goo building up on top of the agar.  Couldn't get a good pic but I'm hoping this is a good sign. A few side notes. I used cheap Wal-Mart instant potatoes which I think was a mistake. There are chunks of meat in it which it didn't advertise but its my fault I should have read the ingredients. Anyway the agar is, well, chunky lets say. Anyhow I hope it still works and will update on progress.


--------------------
I am a rat who lives in a tree stump in the woods. I have my friends over for tea. We sing songs, drink, and be merry.


Extras: Filter Print Post Top
InvisibleJosex
#cheat_code
 User Gallery

Registered: 11/13/15
Posts: 8,995
Loc: Flag
Trusted Cultivator
Re: Pasty Agar Tek [Re: D.little91]
    #24218432 - 04/04/17 04:44 PM (6 years, 10 months ago)

Quote:

D.little91 said:
Hello everyone. So I followed the steps and here I am about a week later and it appears there is some milky colored goo building up on top of the agar.  Couldn't get a good pic but I'm hoping this is a good sign. A few side notes. I used cheap Wal-Mart instant potatoes which I think was a mistake. There are chunks of meat in it which it didn't advertise but its my fault I should have read the ingredients. Anyway the agar is, well, chunky lets say. Anyhow I hope it still works and will update on progress.




No, it's not a good sign, that's bacteria, toss the plate.


Extras: Filter Print Post Top
InvisibleComebackKid
Multispore Enthusiast
Male User Gallery


Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
Trusted Cultivator
Re: Pasty Agar Tek [Re: Josex]
    #24218496 - 04/04/17 05:10 PM (6 years, 10 months ago)

I feel like instant potatoes would have high sodium content no?
Either way what yoyre describing sounds like bacteria


--------------------
:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself


Extras: Filter Print Post Top
OfflineOrganic_Magic
Medicine Man
Male


Registered: 10/26/14
Posts: 1,712
Loc: Everywhere
Last seen: 2 years, 7 months
Re: Pasty Agar Tek [Re: ComebackKid]
    #24218752 - 04/04/17 06:58 PM (6 years, 10 months ago)

you gotta get the plain jane OG potato flakes man.


--------------------
:pipesmoke:Its all fun and games until mushrooms make you gay:pipesmoke:




            click and learn.

Bod's simplified cultivation methods
Got side pins?


Extras: Filter Print Post Top
Jump to top Pages: < First | < Back | 255 | 256 | 257 | 258 | 259 | 260 | 261 | 262 | 263 | 264 | 265 | 266 | 267 | 268 | 269 | 270 | 271 | 272 | 273 | 274 | 275 | Next > | Last >

Shop: Left Coast Kratom Buy Kratom Extract   Myyco.com Isolated Cubensis Liquid Culture For Sale   MagicBag.co All-In-One Bags That Don't Suck   North Spore North Spore Mushroom Grow Kits & Cultivation Supplies   Mushroom-Hut Substrate Bags   Original Sensible Seeds Bulk Cannabis Seeds


Similar ThreadsPosterViewsRepliesLast post
* Agar Tek Aristar 5,833 12 08/01/03 06:42 PM
by shirley knott
* ?NO,AGAR TEK AT SHROOMERY?? bluelou 5,750 8 12/13/19 06:16 PM
by mushboy
* Re: Peroxidated Agar Tek sparks 4,561 6 05/08/00 12:43 AM
by Anonymous
* agar tek? the man 2,777 4 12/16/02 10:45 AM
by the man
* NEW Tissue Culture Technique - No agar, No flow hood, easy as hell
( 1 2 all )
funkyballoon 21,322 38 05/11/01 11:08 AM
by MAIA
* Mycelium-only tek, Please Help! FaminedShaman 2,567 4 05/06/01 06:49 AM
by egghead
* Antibac Agar, DIY! superduper 4,871 13 12/04/02 02:20 PM
by superduper
* agar cultivation - easy! felixhigh 2,498 3 11/28/01 01:16 AM
by BBShrizz

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
797,456 topic views. 23 members, 171 guests and 24 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.034 seconds spending 0.014 seconds on 14 queries.