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OfflineD.little91
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Re: Pasty Agar Tek [Re: shumer44]
    #24206289 - 03/30/17 06:48 PM (6 years, 10 months ago)

Hey guys getting started on this tonight. My pressure cooker says it's operating temp is 12 ppsi. Is that okay? How long do I cook at that temp?


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InvisibleJosex
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Re: Pasty Agar Tek [Re: D.little91]
    #24206311 - 03/30/17 06:57 PM (6 years, 10 months ago)

Quote:

shumer44 said:
I am planning to make 8 plates using the mini rounds tomorrow.

1 drop of liquid from spore syringe per plate is enough right?




That's right

Quote:

D.little91 said:
Hey guys getting started on this tonight. My pressure cooker says it's operating temp is 12 ppsi. Is that okay? How long do I cook at that temp?



The same time it says in the OP will be more than enough, 45 min.


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OfflineD.little91
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Re: Pasty Agar Tek [Re: Josex]
    #24206327 - 03/30/17 07:06 PM (6 years, 10 months ago)

Nice! Okay last question: how much water do I put in the pc?


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InvisibleJosex
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Re: Pasty Agar Tek [Re: D.little91]
    #24206332 - 03/30/17 07:08 PM (6 years, 10 months ago)

That depends on how big your pc is. If you still keep the instructions that came with it, read em.


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OfflineD.little91
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Re: Pasty Agar Tek [Re: Josex]
    #24206466 - 03/30/17 07:59 PM (6 years, 10 months ago)

It just says pour the amount the recipe calls for. It's 8 quart


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OfflineD.little91
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Re: Pasty Agar Tek [Re: D.little91]
    #24206514 - 03/30/17 08:10 PM (6 years, 10 months ago)

I'm quite confused about putting them in the PC. Do they just sit in the water?


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InvisibleComebackKid
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Re: Pasty Agar Tek [Re: D.little91]
    #24206533 - 03/30/17 08:18 PM (6 years, 10 months ago)

Quote:

D.little91 said:
It just says pour the amount the recipe calls for. It's 8 quart




You need a metal rack or something in the bottom of your pc for the plates to sit on
Just put in enough water so your plates aren't floating around on the rack. An inch will usually do.
If it's your first time using a PC make sure you run it on the lowest possible temperature and your rocker weight bobs a few times a minute
If your rocker is going HAM and spitting steam everywhere, then your pc will dry out and eventually warp.


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Invisiblestareatclouds
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Re: Pasty Agar Tek [Re: ComebackKid]
    #24206672 - 03/30/17 09:11 PM (6 years, 10 months ago)

If I'm seeing little satellite colonies a full week after myc transfers, what's the likely culprit? They're happening in both Pasty Plate's (with parafilm around them) and glass 1/2 pints with SFDs/surgical tape.

The actual myc growth looks great, but a week after initial transfer, tiny satellites pop up kinda far away from the myc growth.

This hasn't happened to my petri dishes.


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InvisibleComebackKid
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Re: Pasty Agar Tek [Re: stareatclouds]
    #24206715 - 03/30/17 09:38 PM (6 years, 10 months ago)

Are you fucking around with your wedge in the recieving dish?
I know it can be a little frustrating sometimes getting the wedge off my scalpel and I end up repositioning the wedge to the center of the dish. Usually when this happens I'll get satellite colonies.

I think that would also explain why you're only seeing this in your no pour dishes...
It's likely just cube myc if that's the case


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:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
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Invisiblestareatclouds
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Re: Pasty Agar Tek [Re: ComebackKid]
    #24206819 - 03/30/17 10:29 PM (6 years, 10 months ago)

I do sometimes have to fuck around to get the wedge off my blade, more often than not in the PP's and 1/2 pints because they have higher walls. Looking back, a few of them I had a horrible time with. I meant to mark them. But it seems weird they'd grow so much later than the initial transfer. I know there's varying genetics, but with so many even on that little wedge, I'd like to think it'd happen before 7 days.

Some of them I've just said, "fuck it" and flicked the wedge into it when it's too high on the blade to come off. But usually note these ones. I'm going to hope it was indeed me shuffling the blade around a bit.


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Offlineshumer44
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Re: Pasty Agar Tek [Re: stareatclouds]
    #24206882 - 03/30/17 11:08 PM (6 years, 10 months ago)

If i drill the hole onto the side of mini round, i dont need the aluminum foil at all.

.. then the mini rounds stay on the rack.. wont they melt from the heat inside?


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InvisibleTheMadHatter420
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Re: Pasty Agar Tek [Re: shumer44]
    #24207077 - 03/31/17 01:02 AM (6 years, 10 months ago)

If you stab your wedge just right, you just push down on the agar, your blade cuts its way through wedge into agar, slip free and wedge is in center. Take a couple blank plates and play with it for practice. I have done ALOT better since I figured it out. Also I found that bigger wedges were a lot harder to get off the scalpel than the smaller ones were.


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Invisiblestareatclouds
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Re: Pasty Agar Tek [Re: TheMadHatter420]
    #24207121 - 03/31/17 01:54 AM (6 years, 10 months ago)

My issue is with the blade slicing through the wedge as I plunge it into the new plate at a high angle (due to PP). I also use an X-acto which isn't as good as a scalpel, but whatever.


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InvisibleJosex
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Re: Pasty Agar Tek [Re: TheMadHatter420]
    #24207136 - 03/31/17 02:15 AM (6 years, 10 months ago)

Quote:

TheMadHatter420 said:

If you stab your wedge just right, you just push down on the agar, your blade cuts its way through wedge into agar, slip free and wedge is in center. Take a couple blank plates and play with it for practice. I have done ALOT better since I figured it out. Also I found that bigger wedges were a lot harder to get off the scalpel than the smaller ones were.




:kaneclap: :raisemyglass:
Fucking right! Everything TheHater said to the T.

I've been meaning to say that myself but was waiting for an opportunity to read the ol' whine "it's so hard to get the wedge off the scalpel" or "you need to rotate the scalpel like a pro on the receiving plate" or "you need to cool the scapel real well"... bunch of bullshit.

The key to the whole thing is the way you stab the wedge. I cut triangular wedges all the way down to the botton of the plate, stab one of walls of the wedge (not the surface) with the very tip of the blade and pry the wedge out of the agar puck. That's the whole skill you have to master and agar becomes child's play.

The wedge will come right off the blade and on to the blank plate with a swift motion of your hand, making just a little cut on the blank plate... the receiving plate will stay open for a fraction of a second this way.

Also fuck cooling the scalpel on the blank plate, you really don't need that. I heat the blade red hot, and the body of the scalpel a little, get in the SAB and wait like 20 seconds in front of the donor plate to minimize air currents, then go directly to cut the wedge.

Another trick to make agar even easier is to grab the scalpel from the butt, like a paint brush and use it that way, it will make more precise cuts when you get used to it.

Also, work with the no-pours upside-down, the way Munchauzen does in his videos.


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Re: Pasty Agar Tek [Re: Josex]
    #24207740 - 03/31/17 10:07 AM (6 years, 10 months ago)

Can i use flavored mashed potatoes flakes? Like roasted garlic, etc.. i cant seem to find the normal or original flavor.

Also, there are scalloped potatoes flakes, will this work?


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OfflineKenetic
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Re: Pasty Agar Tek [Re: shumer44]
    #24207751 - 03/31/17 10:12 AM (6 years, 10 months ago)

Another agar tip is just to practice.  A lot.


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InvisibleTheMadHatter420
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Re: Pasty Agar Tek [Re: shumer44]
    #24207762 - 03/31/17 10:18 AM (6 years, 10 months ago)

Normal potato flakes will come in a big box. Will be right by all those flavored fuckers. ALL stores have them. Your just not looking around. Usuall top or bottom shelf in my experiance.


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InvisiblePastywhyteMDiscord
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Re: Pasty Agar Tek [Re: shumer44]
    #24207779 - 03/31/17 10:26 AM (6 years, 10 months ago)

Quote:

shumer44 said:
Can i use flavored mashed potatoes flakes? Like roasted garlic, etc.. i cant seem to find the normal or original flavor.

Also, there are scalloped potatoes flakes, will this work?




Flavored flakes are full of salt which is bad for the culture.


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Invisiblemynakedrat
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Re: Pasty Agar Tek [Re: Pastywhyte]
    #24207803 - 03/31/17 10:34 AM (6 years, 10 months ago)

Scalloped garlic shrooms... Ohhh Mmm :homer:


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Offlinetryptkaloids
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Re: Pasty Agar Tek [Re: mynakedrat]
    #24207923 - 03/31/17 11:27 AM (6 years, 10 months ago)

softer agar sticks to the blade easier


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