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OfflineNatrldlite
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First Grow!
    #24186146 - 03/23/17 01:12 PM (7 years, 6 days ago)

Hello good people! I've lurked forever but finally decided to post since I've started my first grow (pf tek, cubies). I noc'd the jars last Saturday and when I went to peek at them yesterday, 4 days later, I was pleasantly surprised to see the mycelium starting to colonize already. (Hopefully the pic uploads..) I'm pretty pumped about the process! I was wondering if anyone could hazard a guess at this point re: how soon contamination would manifest if it were present. Would I know by now if any of my jars were contaminated or does that take more time to ascertain??

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InvisiblemushboyMDiscord
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Re: First Grow! [Re: Natrldlite]
    #24186155 - 03/23/17 01:16 PM (7 years, 6 days ago)

you would know by how you inoculated the jars and what process you followed.

explain that instead of how pumped you are. with all respect.

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OfflineNatrldlite
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Re: First Grow! [Re: mushboy]
    #24186203 - 03/23/17 01:36 PM (7 years, 6 days ago)

Steam sterilized for 90 minutes. Jars cooled for ~36 hours. I used a still air box in an area of the house unaffected by the AC, it was disinfected using clorox wipes, as were the walls adjacent to the work area. The spore syringe was opened inside the box and that's also where I removed the foil from the jars. I flame sterilized the needle with a scented candle (bad move?) between the first 4 jars and used an alcohol swab on the needle between the 5th and 6th jars.  The jars stayed in the clear sterlite box for 24 hours at which time 4 were placed in a hall closet and 2 were placed in my garage (curious to see if temp difference between the two areas makes a big difference). The inoculation holes have no tape over them but there is a layer of dry verm over the substrate.

I'd imagine the amount of time for contaminants to pop up would depend on the biology of whatever contaminant it was, but I'm curious in general about the colonization rates of the common ones like trich  relative to mycelium.

I'm pumped.

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OfflineCapnZ
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Re: First Grow! [Re: mushboy]
    #24186211 - 03/23/17 01:39 PM (7 years, 6 days ago)

Quote:

mushboy said:
you would know by how you inoculated the jars and what process you followed.

explain that instead of how pumped you are. with all respect.



:whathesaid:

Some details / breakdown on your process would be helpful. Did you use a PC, how did you make your BRF jars, how did you inoculate. Conditions where you keep your jars and yes absolutely a pic of the growth you are seeing. I would say your 1st attempt to upload did not work.

The following is a very general statement, but it's probably too early to tell if any contamination is present. Need to see the pics.


--------------------
Deep into the darkness peering, long I stood there wondering, fearing, doubting, dreaming dreams no mortal ever dared to dream before...

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OfflineCapnZ
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Re: First Grow! [Re: CapnZ]
    #24186218 - 03/23/17 01:42 PM (7 years, 6 days ago)

OK that helps. Yeah I would stay away from the scented candle and at least use a bic lighter. I like a propane torch - way faster. Need to flame sterilize then needle between EACH injection, not just between jars. A lot of what you are doing is good or close, just need to tighten up your technique.

For temps, keep your jars around 70 F.

Oh yeah - PICS!!!


--------------------
Deep into the darkness peering, long I stood there wondering, fearing, doubting, dreaming dreams no mortal ever dared to dream before...

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OfflineThatsjustmybobo
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Re: First Grow! [Re: CapnZ]
    #24186229 - 03/23/17 01:49 PM (7 years, 6 days ago)

Quote:

CapnZ said:
OK that helps. Yeah I would stay away from the scented candle and at least use a bic lighter. I like a propane torch - way faster. Need to flame sterilize then needle between EACH injection, not just between jars. A lot of what you are doing is good or close, just need to tighten up your technique.

For temps, keep your jars around 70 F.

Oh yeah - PICS!!!




Why flame after ea hole? You've got to figure if you've sterilized correctly you shouldn't come into any contams between holes of a single jar. I flame between jars but not holes. No contams yet.
Also I feel as though you'd waste a lot of time flaming every hole, that and the you're going to be doing a lot of waiting for the needle to cool cause you don't wanna cook your spores.

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OfflineNatrldlite
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Re: First Grow! [Re: CapnZ]
    #24186236 - 03/23/17 01:52 PM (7 years, 6 days ago)

Message from the shroomery says that I can't upload the file type when I try to post, but it's just a phone pic?

If 70 degrees is ideal then I should move them all this evening.  The ones in the hall closet are around 74 and the ones in the garage are near 80. Would those be temps more appropriate for fruiting, not needed now?

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Re: First Grow! [Re: Thatsjustmybobo]
    #24186242 - 03/23/17 01:53 PM (7 years, 6 days ago)

Quote:

Thatsjustmybobo said:You've got to figure





thats how noobs fail.

flaming a needle takes 2 seconds?

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OfflineCapnZ
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Re: First Grow! [Re: Natrldlite]
    #24186247 - 03/23/17 01:56 PM (7 years, 6 days ago)

Quote:

Natrldlite said:
Message from the shroomery says that I can't upload the file type when I try to post, but it's just a phone pic?

If 70 degrees is ideal then I should move them all this evening.  The ones in the hall closet are around 74 and the ones in the garage are near 80. Would those be temps more appropriate for fruiting, not needed now?



Stay around 70 F for the entire process. You will be fine.


--------------------
Deep into the darkness peering, long I stood there wondering, fearing, doubting, dreaming dreams no mortal ever dared to dream before...

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OfflineCapnZ
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Re: First Grow! [Re: mushboy]
    #24186249 - 03/23/17 01:56 PM (7 years, 6 days ago)

Quote:

mushboy said:
Quote:

Thatsjustmybobo said:You've got to figure





thats how noobs fail.

flaming a needle takes 2 seconds?




:whathesaid:


--------------------
Deep into the darkness peering, long I stood there wondering, fearing, doubting, dreaming dreams no mortal ever dared to dream before...

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OfflineCapnZ
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Re: First Grow! [Re: Thatsjustmybobo]
    #24186255 - 03/23/17 01:59 PM (7 years, 6 days ago)

Quote:

Thatsjustmybobo said:



Why flame after ea hole? You've got to figure if you've sterilized correctly you shouldn't come into any contams between holes of a single jar. I flame between jars but not holes. No contams yet.
Also I feel as though you'd waste a lot of time flaming every hole, that and the you're going to be doing a lot of waiting for the needle to cool cause you don't wanna cook your spores.




Read some more teks my friend. I think 10 out of 10 growers who know their shit would agree. Flame sterilize between each injection period.


--------------------
Deep into the darkness peering, long I stood there wondering, fearing, doubting, dreaming dreams no mortal ever dared to dream before...

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InvisiblemushboyMDiscord
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Re: First Grow! [Re: CapnZ]
    #24186256 - 03/23/17 02:00 PM (7 years, 6 days ago)

even if you dont need too, its just good practice for good technique.

agar work is a skill. you have to learn a skill. few people are cranking out drum solos while learning to
keep the beat with a high hat.:cookiemonster:

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OfflineThatsjustmybobo
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Re: First Grow! [Re: mushboy]
    #24186264 - 03/23/17 02:07 PM (7 years, 6 days ago)

Quote:

mushboy said:
Quote:

Thatsjustmybobo said:You've got to figure





thats how noobs fail.

flaming a needle takes 2 seconds?




Okay then I'll put it like this: if a jar is contaminated, no amount of flaming from hole to hole will save it.

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Re: First Grow! [Re: Thatsjustmybobo]
    #24186269 - 03/23/17 02:08 PM (7 years, 6 days ago)

what if a contam falls on the needle while its moving from one hole to the other. if you flame it.....

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OfflineNatrldlite
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Re: First Grow! [Re: mushboy]
    #24186288 - 03/23/17 02:13 PM (7 years, 6 days ago)

If flame sterilization is recommended between holes vs between jars then I missed that step somewhere. It doesn't seem as logical to me but I don't guess I see what it'd hurt. Gotta be better than ruined cakes.

Is there a window beyond which contaminants are less likely? Any pattern to when they might generally show up? If the jars weren't well sterilized, for example, I'd expect to see them already, yes? Whereas if it occurred during inoculation, they may not be? Are air bornes that may float down into the hole sufficiently blocked via the verm layer? I guess I'm just asking, for those who've dealt with contamination in the past, how and when are most common scenarios

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OfflineNatrldlite
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Re: First Grow! [Re: Natrldlite]
    #24186289 - 03/23/17 02:13 PM (7 years, 6 days ago)

And why can't I post a pic?!

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OfflineThatsjustmybobo
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Re: First Grow! [Re: mushboy]
    #24186291 - 03/23/17 02:14 PM (7 years, 6 days ago)

Well if you've got your sab, and you're not just jabbin at random things, youve cleaned the injection points...
I understand it's good technique, I just don't believe it's 100%  necessary.

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Re: First Grow! [Re: Thatsjustmybobo]
    #24186301 - 03/23/17 02:20 PM (7 years, 6 days ago)

to be perfectly honest i use grains and do not use needles or verm layers and shit so my opinion on the matter is bias i guess.

Quote:

Thatsjustmybobo said:
I understand it's good technique, I just don't believe it's 100%  necessary.




right their with you. id rather stress good tech than a believe thats all.

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Offlinegrzyp
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Re: First Grow! [Re: Natrldlite]
    #24186305 - 03/23/17 02:21 PM (7 years, 6 days ago)

Quote:

Natrldlite said:
And why can't I post a pic?!



https://www.shroomery.org/forums/showflat.php/Number/13674858

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OfflineThatsjustmybobo
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Re: First Grow! [Re: mushboy]
    #24186322 - 03/23/17 02:26 PM (7 years, 6 days ago)

Quote:

mushboy said:
to be perfectly honest i use grains and do not use needles or verm layers and shit so my opinion on the matter is bias i guess.

Quote:

Thatsjustmybobo said:
I understand it's good technique, I just don't believe it's 100%  necessary.




right their with you. id rather stress good tech than a believe thats all.




No you're right to stress proper tech, I absolutely respect and agree with that.

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OfflineNatrldlite
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Re: First Grow! [Re: grzyp]
    #24186347 - 03/23/17 02:37 PM (7 years, 6 days ago)


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OfflineNatrldlite
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Re: First Grow! [Re: Natrldlite]
    #24186353 - 03/23/17 02:38 PM (7 years, 6 days ago)

That's the only close uproximity, taken yesterday. They all looked the same.

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Invisiblemynakedrat
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Re: First Grow! [Re: Natrldlite]
    #24186366 - 03/23/17 02:43 PM (7 years, 6 days ago)

Looking Good bro! Don't open them! Lol don't eat them Yet!
I know Your tempted.

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OfflineNatrldlite
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Re: First Grow! [Re: mynakedrat]
    #24186459 - 03/23/17 03:25 PM (7 years, 6 days ago)

I can't say that I'm "tempted" at this stage, but I'm damn sure anxious!

Can anyone say what precautions I should take in moving them to a cooler and cleaner area?,

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OfflineCapnZ
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Re: First Grow! [Re: Thatsjustmybobo]
    #24186560 - 03/23/17 03:54 PM (7 years, 6 days ago)

Quote:

Thatsjustmybobo said:
Quote:

CapnZ said:
OK that helps. Yeah I would stay away from the scented candle and at least use a bic lighter. I like a propane torch - way faster. Need to flame sterilize then needle between EACH injection, not just between jars. A lot of what you are doing is good or close, just need to tighten up your technique.

For temps, keep your jars around 70 F.

Oh yeah - PICS!!!




Why flame after ea hole? You've got to figure if you've sterilized correctly you shouldn't come into any contams between holes of a single jar. I flame between jars but not holes. No contams yet.
Also I feel as though you'd waste a lot of time flaming every hole, that and the you're going to be doing a lot of waiting for the needle to cool cause you don't wanna cook your spores.




Do what you want man. It's your grow. Me, I flame every time. Just IMO it's the right thing to do. I get very little if any contamination so it works for me.


--------------------
Deep into the darkness peering, long I stood there wondering, fearing, doubting, dreaming dreams no mortal ever dared to dream before...

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OfflineCapnZ
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Re: First Grow! [Re: Natrldlite]
    #24186567 - 03/23/17 03:56 PM (7 years, 6 days ago)

Quote:

Natrldlite said:




Yeah looks good! Let em grow and post another pic in a week or so. Just (try to) forget about them for a while. :rockon:


--------------------
Deep into the darkness peering, long I stood there wondering, fearing, doubting, dreaming dreams no mortal ever dared to dream before...

Edited by CapnZ (03/23/17 03:56 PM)

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OfflineNatrldlite
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Re: First Grow! [Re: CapnZ]
    #24186770 - 03/23/17 05:27 PM (7 years, 6 days ago)

A week may not be realistic given my anticipation, but I can probably wait until at least Tuesday or Wednesday. And I'll definitely be throwing up some pics as I move through the remaining stages, right through to the trip report

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OfflineNatrldlite
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Re: First Grow! [Re: Natrldlite]
    #24200216 - 03/28/17 04:01 PM (7 years, 1 day ago)

I waited as long as I could before peeking.  I didn't want to mess with them much beyond that but I did photograph 3 of them (including the bottom of one that seemed to be doing particularly well). Not sure if I captured it or not but there appears to by a little condensation in at least one of the jars. Is that normal? Problematic? These 3 jars (4 photos) are representative of the six, all seem to look the same.  The primary difference in them being differential colonization rates. What do you knowledgeable folk think?

Editing to add that this is 10 days post inoculation.








Edited by Natrldlite (03/28/17 04:03 PM)

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OfflineNatrldlite
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Re: First Grow! [Re: Natrldlite]
    #24213899 - 04/02/17 06:59 PM (6 years, 11 months ago)

So this is what most of the jars look like now. Most others I've seen looked a lot smoother around d the outside. Mine don't have that smoother appearance, it looks more like myceliun run amok. Any thoughts??






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OfflineMadSeasonStudent
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Re: First Grow! [Re: Natrldlite]
    #24214086 - 04/02/17 08:32 PM (6 years, 11 months ago)

Those looks really great!!!
Getting ready for birthing really soon.


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OfflineNatrldlite
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Re: First Grow! [Re: MadSeasonStudent]
    #24214992 - 04/03/17 10:53 AM (6 years, 11 months ago)

So the cakes I've seen in others' photos and videos that were much smoother looking around the outside, they had simply formed better to the jars during the last week or two prior to birthing? Mine seem to have an inordinate topography going on at this stage compared to what I was expecting...

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Re: First Grow! [Re: CapnZ]
    #24216473 - 04/03/17 09:02 PM (6 years, 11 months ago)

So I have 3 that are 100% colonized, i plan to birth them this weekend. Two others are about 90%, they may wait until the following weekend. The 6th jar has stalled out at about 40%. I couldn't say why, but the top verm layer looks like it shifted some. It's quarantined in case it's due to contamination until I can see what it does, if anything. Is there any hope to try something else with that jar? Spawn it to something else maybe? And what advice would you give regarding my birthing plans for the others?

Thanks for any input anyone may have!

Quote:

CapnZ said:
Quote:

Natrldlite said:




Yeah looks good! Let em grow and post another pic in a week or so. Just (try to) forget about them for a while. :rockon:



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