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Invisiblebastard4life
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APE syringe looks wierd
    #24182841 - 03/22/17 10:42 AM (6 years, 10 months ago)

I received an APE syringe from a sponsor. I've got them before. They always were pure white spores. This time there white but have black specs on the outside of the spore ball and a white milky film floating around the spore ball. Should I be worried? I called the vendor who told me that the black is common. He told me that the white is most likely spores that have germinated. He also said if there is a problem upon closer inspection he will send a replacement. Im curious if someone can confirm what he said. I trust the vendor but i want reassurance. This one always gets away from me somehow and I really want to keep it. I put a drop on agar already to see what happens.


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Offlinemushpunx
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Re: APE syringe looks wierd [Re: bastard4life]
    #24182937 - 03/22/17 11:18 AM (6 years, 10 months ago)

They can germinate in solution. And I forget how they do PE syringes but its different than normal due to the difficulty in getting good spore prints.

Anyone know? Something with a centerfuge?


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Invisiblebastard4life
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Re: APE syringe looks wierd [Re: mushpunx]
    #24182939 - 03/22/17 11:20 AM (6 years, 10 months ago)

I've heard centrifuge. Which makes me wonder about the black spots.


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InvisibleComebackKid
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Re: APE syringe looks wierd [Re: bastard4life]
    #24182958 - 03/22/17 11:26 AM (6 years, 10 months ago)

Its probably just charcoal. Very common.
My APE syringe didn't have any black inside, but it had a white cloud inside which I assumed was just spores at the time.
Drop it to agar, I bet it's clean as fuck :awesomenod:


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Invisiblebastard4life
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Re: APE syringe looks wierd [Re: ComebackKid]
    #24182969 - 03/22/17 11:30 AM (6 years, 10 months ago)

Quote:

ComebackKid said:
Its probably just charcoal.
Drop it to agar, I bet it's clean as fuck :awesomenod:



I did as soon as I got it... after I talked to the vendor. He didnt say anything about charcoal. And im sure its clean. Just curious because this is my 5th APE and first time looking like this. All others were pure white. What's charcoal for?


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InvisibleMoabfighter
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Re: APE syringe looks wierd [Re: bastard4life]
    #24183009 - 03/22/17 11:42 AM (6 years, 10 months ago)

My ape syringe looks like someone splooged in it.


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Offlinelovelaughlibs
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Re: APE syringe looks wierd [Re: mushpunx]
    #24183014 - 03/22/17 11:44 AM (6 years, 10 months ago)

Quote:

mushpunx said:
They can germinate in solution. And I forget how they do PE syringes but its different than normal due to the difficulty in getting good spore prints.

Anyone know? Something with a centerfuge?



I think they kinda slurry the fruits and centrifuge them and all the spores seperate into their own layer, could be wrong though.


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Invisiblebastard4life
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Re: APE syringe looks wierd [Re: lovelaughlibs]
    #24183031 - 03/22/17 11:48 AM (6 years, 10 months ago)

Quote:

lovelaughlibs said:

I think they kinda slurry the fruits and cliterally e them and all the spores seperate into their own layer, could be wrong though.



your probably right. He told me specifically not to worry about the black spots. He literally said ignore them and when I asked again he told me if there's any problem I'll get a replacement. Probably some secret he won't let out and I don't blame him.


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Invisiblebastard4life
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Re: APE syringe looks wierd [Re: bastard4life]
    #24183038 - 03/22/17 11:52 AM (6 years, 10 months ago)

The charcoal must help with the microscopic viewing of the spores.  I can't think of any other reason for its presence without implying intent to cultivate.  Good luck!

^^^^^^This is a quote from workman. Know it makes sense because he made it and want people to view it.


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InvisibleComebackKid
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Re: APE syringe looks wierd [Re: bastard4life]
    #24183679 - 03/22/17 03:17 PM (6 years, 10 months ago)

Activated charcoal has antibacterial properties which could arguably be justified for microscopy purposes. It just so happens to help with spore germination as well:shrug:


--------------------
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Look around you... Everything you see exists inside the mind.
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Our individual experiences are all part of the universe's experience of itself


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Invisiblebastard4life
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Re: APE syringe looks wierd [Re: ComebackKid]
    #24183692 - 03/22/17 03:22 PM (6 years, 10 months ago)

I'm guessing it also makes albino spores stand out since charcoal is black and albino spores are "clear" wish I had a scope. I'm bumming about not having one.


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InvisibleTheMadHatter420
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Re: APE syringe looks wierd [Re: bastard4life]
    #24183700 - 03/22/17 03:25 PM (6 years, 10 months ago)

I'm in the same boat. I want one bad, but don't have the funds currently.


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InvisibleComebackKid
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Re: APE syringe looks wierd [Re: TheMadHatter420]
    #24183766 - 03/22/17 03:52 PM (6 years, 10 months ago)

It would be especially interesting to determine if the cloud in your syringe was a clump of spores or if it is actually mycelium floating around...

I don't see why spores would germinate while supended in water.  There's no nutrients in the water so it doesn't make sense..

One thing I do know is that APE is notorious for "germinating" in the syringe.
It is also the only variety available that has true albino spores....


--------------------
:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself


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InvisibleMad Season
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Re: APE syringe looks wierd [Re: TheMadHatter420]
    #24183768 - 03/22/17 03:53 PM (6 years, 10 months ago)

You can get some pretty fuckin cheap USB microscopes as long as you don't mind looking at it on a computer screen :smile:


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Invisiblebastard4life
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Re: APE syringe looks wierd [Re: Mad Season]
    #24183793 - 03/22/17 04:03 PM (6 years, 10 months ago)

Quote:

Mad Season said:
You can get some pretty fuckin cheap USB microscopes as long as you don't mind looking at it on a computer screen :smile:



How well do they work? I'd like to see spores and mycelium under a scope. I can always hook the comp up to my 42in hdtv


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InvisibleMad Season
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Re: APE syringe looks wierd [Re: bastard4life]
    #24183821 - 03/22/17 04:16 PM (6 years, 10 months ago)

Quite well actually. Most can do at least 400x and come with their own stand so you're not holding it. Although it's great too because you can hold it and sometimes look through jars at the contam spores to identify those as well


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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Invisiblebastard4life
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Re: APE syringe looks wierd [Re: Mad Season]
    #24183862 - 03/22/17 04:27 PM (6 years, 10 months ago)

So this 1000x digital usb microscope I'm looking at for $20 would be a good idea. Cool I know what my next little bit of cash is going towards.


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Offlinemushpunx
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Re: APE syringe looks wierd [Re: bastard4life]
    #24184038 - 03/22/17 05:32 PM (6 years, 10 months ago)

Id be into getting a microscope for a decent price. I dunno how well I'd be able to apply it but I could learn :shrug: at the very least have some fin with it


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Invisiblebastard4life
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Re: APE syringe looks wierd [Re: mushpunx]
    #24184604 - 03/22/17 09:52 PM (6 years, 10 months ago)

http://m.ebay.com/itm/2MP-1000X-8-LED-USB-Digital-Microscope-Endoscope-Zoom-Camera-Magnifier-Stand-/391444129697?nav=WATCHING_ACTIVE
Is this a good investment for seeing the shape and color of spores? I'd really like to see the different species and don't want to make a foolish purchase.


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OfflineWorkmanV
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Re: APE syringe looks wierd [Re: bastard4life]
    #24186096 - 03/23/17 12:57 PM (6 years, 10 months ago)

I'm guessing that the black specks are normal colored spores which occasionally occur in albino strains. When this happens it is usually on just a few gills and not the entire mushroom. Normally, any fruits with black spores are rejected but it is possible that a few made it past quality control. No charcoal in any case.


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