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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,649
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Re: Pastywhyte's Easy Agar Tek [Re: Germs]
#24180448 - 03/21/17 01:36 PM (6 years, 10 months ago) |
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it's regulated by heat, your pressure cooker can sit there sealed all day and it won't build any pressure, I quite often turn the heat all the way up immediately, if they are out of the water they won't get fucked. the paper towel will come out soaked but that's fine. I'm just often in a hurry and don't have time to babysit the pressure cooker. I'd rather turn it on wait for it to start rocking, turn it down a little and leave for a half hour. but if you have the time I suggest turning it on low, waiting 10 mins and turning it up slightly every 10 minutes until it starts rocking
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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AK1000
Stranger



Registered: 06/06/16
Posts: 488
Last seen: 2 years, 6 months
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This one is for you pasty: a AA941 PC filled with pasty's... About 40 of them. I was pissed when I realized I could've fit 12 more but ran out of pasty's.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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Re: Pastywhyte's Easy Agar Tek [Re: AK1000]
#24181272 - 03/21/17 06:22 PM (6 years, 10 months ago) |
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That's pretty boss
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VermicuLad
Stranger

Registered: 12/15/16
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Last seen: 6 years, 10 months
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Re: Pastywhyte's Easy Agar Tek [Re: Pastywhyte]
#24182318 - 03/22/17 04:45 AM (6 years, 10 months ago) |
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I created my first pasty plates and squirted old unfridged LC in there. I squirted too much from the syringe so the whole plates were covered in it, negating the purpose of the agar surface. Mycelium grew all over the plate but it could not be called sterile. I could use an inoculation loop next time if I ever use the LC again, but I would have to learn how. Maybe squirt some in a sterilized bottle cap and dip in the loop?
I created 8 new plates. 4 got small chunks from the previous plates and look fantastic so far. The other 4 got hastily scraped spores from a crappy print I made, and mycelium can now be seen on at least 3 of them. I plan to make a blenderless liquid inoculate from one of each group to make two 32 qt. no-hole monotubs with WBS and coir (first time doing a bulk so I still need to read about casing and stuff).
For the plates I used no foil or paper on top and put the hole and tape on the side. I have both glads and ziplocs. The square ziplocs seem to stack much easier than the glad rounds and maybe resist getting crushed by the pressure a bit better.
I did a lot of dumb things and fiddling around too much in the SAB when making the 8 latest plates, but it seemed to work out. The tek is easy. It was easier than my LC was with its ghetto silicone injection ports that required me to stab the syringe several times to get through the off-center hole. Use agar.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Re: Pastywhyte's Easy Agar Tek [Re: VermicuLad]
#24182453 - 03/22/17 07:53 AM (6 years, 10 months ago) |
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Click my link in my signature then click agar. Then click the comprehensive agar from there's a demonstration of using a swab/loop
https://www.shroomery.org/forums/showflat.php/Number/21922023
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synthetik
biological entity

Registered: 12/15/16
Posts: 194
Last seen: 3 years, 11 months
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Re: Pastywhyte's Easy Agar Tek [Re: AK1000]
#24182487 - 03/22/17 08:13 AM (6 years, 10 months ago) |
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Quote:
AK1000 said: This one is for you pasty: a AA941 PC filled with pasty's... About 40 of them. I was pissed when I realized I could've fit 12 more but ran out of pasty's.

damn those 41's are fucking huge! there one on CL near me for a pretty good price, I just really dont need it... also Im really only here because of your post about your first bottle grow and what NOT to do... just wanted to thanks you again seeing it all put together made it much easier for me to make the leap... I took your advice... waited for pins on my plates, two transfers away to be sure about contams, im actually waiting for my first set of bottles to cool in my sab right now (up all night! the AA is back to 20psi... so yeah at least one person listened to your crazy ideas!
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lovelaughlibs
Dopamine Slave



Registered: 10/14/15
Posts: 1,811
Loc: England
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Re: Pastywhyte's Easy Agar Tek [Re: synthetik]
#24182496 - 03/22/17 08:17 AM (6 years, 10 months ago) |
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Quote:
synthetik said: there one on CL near me for a pretty good price
Dude, go buy it! You never know when your current PC will croak or when you'll wanna upgrade
-------------------- Ask and ye shall receive; Seek and ye shall find.
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synthetik
biological entity

Registered: 12/15/16
Posts: 194
Last seen: 3 years, 11 months
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I have a 1925x and just sold a 1915x... for now ill just stick with this 25x, my place is hella small and this hobby has taken over it all! lol shit I went with the smaller oyster blender at the thrift store just because this shit is getting out of hand...
pasty was right, and I forget his exact quote, but you start with a few plates, couple months later and you have fucking mini rounds everywhere and the lady at the asian market calls you the "agar-agar guy"... fun hobby
when im ready ill get the 41qt steroclave... finding the sweetspot on a hotplate is a weird thing for me... seems like its always changing
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lovelaughlibs
Dopamine Slave



Registered: 10/14/15
Posts: 1,811
Loc: England
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Re: Pastywhyte's Easy Agar Tek [Re: synthetik]
#24182997 - 03/22/17 11:39 AM (6 years, 10 months ago) |
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Quote:
synthetik said: I have a 1925x and just sold a 1915x... for now ill just stick with this 25x, my place is hella small and this hobby has taken over it all! lol shit I went with the smaller oyster blender at the thrift store just because this shit is getting out of hand...
pasty was right, and I forget his exact quote, but you start with a few plates, couple months later and you have fucking mini rounds everywhere and the lady at the asian market calls you the "agar-agar guy"... fun hobby
when im ready ill get the 41qt steroclave... finding the sweetspot on a hotplate is a weird thing for me... seems like its always changing
Ahh yeah I know the feeling lol, I haven't even got a crop yet and there's so many fucking jars, bottles, agar dishes and paraphernalia (PC, SFD etc) strewn about the place.
Lol @ you being the agar-agar guy
-------------------- Ask and ye shall receive; Seek and ye shall find.
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orthiad
Stranger

Registered: 01/11/17
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Last seen: 2 years, 6 months
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Re: Pastywhyte's Easy Agar Tek [Re: Pastywhyte]
#24184439 - 03/22/17 08:31 PM (6 years, 10 months ago) |
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Is it better to just drop the spores from a syringe on the top or to make a small hole with the needle after flame sterilizing?
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,649
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Re: Pastywhyte's Easy Agar Tek [Re: orthiad]
#24184492 - 03/22/17 08:52 PM (6 years, 10 months ago) |
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doesn't matter IMO poking the agar just kind of helps the spores stay where you want them. I only make a hole in the surface if I'm inoculating with an inoculation loop and that's just because I've convinced myself that it gives the spores easier access to the water.. I have nothing to back that assumption up with though.
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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orthiad
Stranger

Registered: 01/11/17
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Last seen: 2 years, 6 months
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Re: Pastywhyte's Easy Agar Tek [Re: orthiad]
#24184518 - 03/22/17 09:04 PM (6 years, 10 months ago) |
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All I have are syringes and I am still working on my first 9 jars of spawn colonizing so trying this out as I just poured 8 plates and letting them cool down so I can pressure cook them in the morning. Just trying to keep myself productive.
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tryptkaloids
Learner



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Re: Pastywhyte's Easy Agar Tek [Re: orthiad]
#24184533 - 03/22/17 09:11 PM (6 years, 10 months ago) |
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they'll be fine overnight as long as you close them. I wouldn't leave them open cuz they'll dry out
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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orthiad
Stranger

Registered: 01/11/17
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Re: Pastywhyte's Easy Agar Tek [Re: orthiad]
#24184546 - 03/22/17 09:18 PM (6 years, 10 months ago) |
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They are all sealed and I can see quite a bit of condensation on the insides so I think I am all set. I think I need to work on my preparing because my last dish didn't get as much as the other 7 I think i added to much honey on accident.
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tryptkaloids
Learner



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Posts: 12,649
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Re: Pastywhyte's Easy Agar Tek [Re: orthiad]
#24184552 - 03/22/17 09:24 PM (6 years, 10 months ago) |
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I honestly don't even use honey. occasionally I'll add a drop to aid germination but generally not necessary. there have been times where I boil too much and end up with as few as 5 plates. you'll dial it down, it's not an exact science. unless you tend on testing different variables (which most can't do effectively as there are too many variables and few have access to a genuine monoculture)
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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shumer44



Registered: 11/15/16
Posts: 255
Last seen: 6 years, 2 days
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1. Can Agar Agar powder be used instead of the bar?
Like this:
http://fiestafarms.ca/13347/food/agar-agar-the-vegan-gelatin-alternative
2. 1 plate of successful agar agar mycelium can be used for multiple grain jars, right?
Edited by shumer44 (03/26/17 02:59 PM)
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
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Re: Pastywhyte's Easy Agar Tek [Re: shumer44]
#24195204 - 03/26/17 04:56 PM (6 years, 10 months ago) |
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Double yep.
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shumer44



Registered: 11/15/16
Posts: 255
Last seen: 6 years, 2 days
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Re: Pastywhyte's Easy Agar Tek [Re: Josex]
#24197777 - 03/27/17 06:09 PM (6 years, 10 months ago) |
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Is Gelatine same as Agar Agar.. Can I use gelatine then?
Here are the pics 1. http://i.imgur.com/2t6lpIs.jpg 2. http://i.imgur.com/6lvJ17E.jpg
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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Re: Pastywhyte's Easy Agar Tek [Re: shumer44]
#24197794 - 03/27/17 06:14 PM (6 years, 10 months ago) |
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No you can't use gelatine.
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shumer44



Registered: 11/15/16
Posts: 255
Last seen: 6 years, 2 days
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Re: Pastywhyte's Easy Agar Tek [Re: Pastywhyte]
#24197816 - 03/27/17 06:23 PM (6 years, 10 months ago) |
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