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alabamamushie
Tmoney


Registered: 01/04/13
Posts: 522
Loc: Alabama
Last seen: 3 years, 8 months
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in need of help possible claviceps paspali?
#24150544 - 03/10/17 05:43 AM (7 years, 2 months ago) |
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so i swabbed like 5 random things and swab it to agar this has been about 14-21 days ago and heres the results of the swab plate and then funky mold with PE transfer plate also keep in mind that the plate was orignally blue agar now its a orangish pinkish orangey brown idk what it is poss claviceps? heres a link to 4x pics of claviceps paspali on agar https://www.shroomery.org/forums/showflat.php/Number/18937982/fpart/1/vc/1
and heres my swab plate i tried to provide different lighting and on top of different surfaces aswell with these pics
held up to the ceiling fan light

under t8 fluoros on top of a clear/opaque tote

different angle of fluoro and tote

top view sitting on top of a white napkin with normal lighting no idea what clear bubbly things are in the middleish of the plate

^ closer up to the right side of the above image

now here is my transfer plate which looks orangey greeny with some type of watery droplets on top of it

different angle

another angle

the swab and the transfer plate side by side comparison
 now thats some strong pigmentation through the agar cause it was solid blue like my pe transfer plate.
i wasn't trying to get claviceps or anything i was just random swabbing one included my cell phone and doing a transfer i guess a spore fell in
but they do kinda resemble a claviceps culture and that would be the best contamination i could ask for really lmao i am hunting for wild ergot to try to culture though my friend has a organic rye field and his pa just had gangrene sounds like some claviceps purpurea havent checked the field yet
as you can tell by my name im all the wayyyyy down south
regardless if anyone could provide some possible ids on these weird contams will be muchly appreciated
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myceliups
Builder



Registered: 01/24/15
Posts: 1,728
Loc: Philthadelphia
Last seen: 1 hour, 19 minutes
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Re: in need of help possible claviceps paspali? [Re: alabamamushie]
#24150666 - 03/10/17 07:25 AM (7 years, 2 months ago) |
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Try posting it in the contamination forum. Pretty knowledgeable folks on the subject of contams.
https://www.shroomery.org/forums/postlist.php/Board/22
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,891
Loc: Milky way
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Re: in need of help possible claviceps paspali? [Re: myceliups]
#24151523 - 03/10/17 01:53 PM (7 years, 2 months ago) |
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Looks like aspergillus
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krypto2000
Unknown


Registered: 12/05/06
Posts: 11,579
Last seen: 4 years, 6 months
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Re: in need of help possible claviceps paspali? [Re: bodhisatta]
#24151610 - 03/10/17 02:36 PM (7 years, 2 months ago) |
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You swabbed random things in hopes of isolating c. paspali? I'm sure you picked up some paspali spores, but that's absolutely not a viable method of isolating them. Unless you have a microscope you can't even identify what's what since you could literally be growing anything. If you swabbed some honeydew or grew from sclerotia then you could at least assume if it looks like paspali it likely is, which also narrows down your possible contaminants, but just swabbing something random you have no clue.
If you're trying to get a culture for ergoloid production then there's not even any hope I'd say as even if you could isolate every claviceps spore you happened to grab they're likely all inactive anyway, it's not like cubes where any random strain probably produces a decent amount of alkaloids, most claviceps strains do not produce an appreciable amount, many none at all.
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alabamamushie
Tmoney


Registered: 01/04/13
Posts: 522
Loc: Alabama
Last seen: 3 years, 8 months
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Re: in need of help possible claviceps paspali? [Re: krypto2000]
#24152125 - 03/10/17 06:11 PM (7 years, 2 months ago) |
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Quote:
krypto2000 said: You swabbed random things in hopes of isolating c. paspali? I'm sure you picked up some paspali spores, but that's absolutely not a viable method of isolating them. Unless you have a microscope you can't even identify what's what since you could literally be growing anything. If you swabbed some honeydew or grew from sclerotia then you could at least assume if it looks like paspali it likely is, which also narrows down your possible contaminants, but just swabbing something random you have no clue.
If you're trying to get a culture for ergoloid production then there's not even any hope I'd say as even if you could isolate every claviceps spore you happened to grab they're likely all inactive anyway, it's not like cubes where any random strain probably produces a decent amount of alkaloids, most claviceps strains do not produce an appreciable amount, many none at all.
i was swabbing randomly for fun to see what all was on the most used everyday things not trying for a culture like i said in my post im hunting for wild ergot yes but not like this lol i just thought it kinda looked like it and woulda been rolling balls laughing if somehow i ended up with it on there lol everywhere i step outside is dallis grass obtaining a sclerotia would be no problem but it wouldnt do me any good to culture except for fun shits and giggles and that im just interested in seeing it grow cause it looks weirdddd
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