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Offlinerev2010
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agar question
    #24144926 - 03/07/17 09:22 PM (7 years, 10 months ago)

so i just received some agar and petri dishes. now i want to make the agar and start learnign how to use it. my question is... my PC is not big enough to use a wiskey bottle or anything like that. i need to get a bigger one but for now it is what it is. can i use a pint jar to make agar in. just use less amounts. or can i just boil the agar mix in the wiskey bottle for and hour or so?

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InvisibleFrank969
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Re: agar question [Re: rev2010]
    #24144937 - 03/07/17 09:27 PM (7 years, 10 months ago)

You can use a pint jar you just wouldn't be able to make that many plates.

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OfflineNextStopWilloughby
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Re: agar question [Re: Frank969]
    #24144956 - 03/07/17 09:39 PM (7 years, 10 months ago)

This looks like a job for PastyWhyte's Easy Agar Tek

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InvisibleetuHad4
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Re: agar question [Re: NextStopWilloughby]
    #24144971 - 03/07/17 09:51 PM (7 years, 10 months ago)

Hopefully by agar you mean...
Agar plus a nutrient such as Light malt extract
I am not sure AGAR alone will work!

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InvisibleZiran
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Re: agar question [Re: NextStopWilloughby]
    #24144979 - 03/07/17 09:56 PM (7 years, 10 months ago)

Quote:

NextStopWilloughby said:
This looks like a job for PastyWhyte's Easy Agar Tek




This. no pours are awesome.

I use this recipe of agar in my no pours
munches agar recipe


--------------------
Song Of Healing
:super: Updated Pf Tek Guide :super:
Ziran's Teks
AMU Q&A Thread
The Chinese word for nature is zìrán and it means that of which is of itself.


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Offlinerev2010
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Re: agar question [Re: etuHad4]
    #24144982 - 03/07/17 09:57 PM (7 years, 10 months ago)

yes it is mea. malt extract agar. a 56 gram packet

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Offlinerev2010
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Re: agar question [Re: rev2010]
    #24144989 - 03/07/17 10:05 PM (7 years, 10 months ago)

and i have sterile petri dishes i just use a bottle neck bottle. its to small for this little pc. so my main question is can i just mix the right amounts of malt agar and water for a pint jar. mix. boil. pc for 30min. and pour from the jar into petri dishes. the jar would have a ship lid on it so it already has a hole for GE

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Invisiblemaddchef
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Re: agar question [Re: rev2010]
    #24145039 - 03/07/17 10:27 PM (7 years, 10 months ago)

I pour all my petris from a quart jar so yes.


250mls will do roughly 10 plates.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

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:havesomescience:

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InvisibleetuHad4
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Re: agar question [Re: rev2010]
    #24145049 - 03/07/17 10:30 PM (7 years, 10 months ago)


malt extract agar plus agar agar?
great video
I did pure MEA with Ok results but adding AGAR AGAR helped me 10 fold

Edited by etuHad4 (03/07/17 10:32 PM)

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Offlinerev2010
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Re: agar question [Re: rev2010]
    #24145060 - 03/07/17 10:36 PM (7 years, 10 months ago)

so i realized i only have malt extract agar. and i also need agar agar?  do they sell it at grocery store or (walmart) or does it have to be from a asain store.

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InvisibleLeftfield420
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Re: agar question [Re: rev2010]
    #24145085 - 03/07/17 10:47 PM (7 years, 10 months ago)

You might find it at a health food place...but Walmart is not going to have it...an Asian store or online is the best bet though

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Offlinerev2010
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Re: agar question [Re: Leftfield420]
    #24145093 - 03/07/17 10:51 PM (7 years, 10 months ago)

ok thanks. and ive been looking for a solid mixing measurements but not finding what im looking for. i am using
MEA
Agar agar
distilled water.
1 pint jar. not quart

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Invisiblemaddchef
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Re: agar question [Re: rev2010]
    #24145187 - 03/07/17 11:45 PM (7 years, 10 months ago)

If you have premixed mea then that's all you need.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:

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Offlinerev2010
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Re: agar question [Re: rev2010]
    #24145212 - 03/08/17 12:13 AM (7 years, 10 months ago)

this is what i have.

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OfflineMarmie
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Re: agar question [Re: rev2010]
    #24145244 - 03/08/17 12:42 AM (7 years, 10 months ago)

You dont need to buy anything else , what you have has agar n nutes premixed. A mickey or mini mickey of absolute vodka works great for a short small necked agar bottle also


--------------------

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Offlinerev2010
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Re: agar question [Re: Marmie]
    #24145250 - 03/08/17 12:45 AM (7 years, 10 months ago)

ok cool. and thanks for the idea. that actually sounds perfect. im excided to start using agar.then i can use lc and speed of the process for my wbs. and that other threat about those rotten none cube shrooms is rediculous. lol. thanks again

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OfflineCapnZ
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Re: agar question [Re: rev2010]
    #24145539 - 03/08/17 05:43 AM (7 years, 10 months ago)

Check out media bottles. No need for a new PC. They come in different sizes. You can find one that fits your PC.


--------------------
Deep into the darkness peering, long I stood there wondering, fearing, doubting, dreaming dreams no mortal ever dared to dream before...

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OfflineCapnZ
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Re: agar question [Re: rev2010]
    #24145552 - 03/08/17 05:50 AM (7 years, 10 months ago)

Quote:

rev2010 said:
ok thanks. and ive been looking for a solid mixing measurements but not finding what im looking for. i am using
MEA
Agar agar
distilled water.
1 pint jar. not quart



Just use the MEA, don't need Agar agar with MEA. 25 grams MEA to 500 ml water. You can scale it from there to whatever amount you want to make.


--------------------
Deep into the darkness peering, long I stood there wondering, fearing, doubting, dreaming dreams no mortal ever dared to dream before...

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Offlinerev2010
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Re: agar question [Re: CapnZ]
    #24145584 - 03/08/17 06:13 AM (7 years, 10 months ago)

OK so I mixed my agar and PC it for 30 min. When I took it out I noticed there where some chunks floating around. I know it's not from anything else. Is that normal. And it's a yellowish color. Not clear

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OfflineCapnZ
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Re: agar question [Re: rev2010]
    #24145600 - 03/08/17 06:35 AM (7 years, 10 months ago)

Should not be clear it should have color that's OK. What temp was it when ou took it out of the PC?


--------------------
Deep into the darkness peering, long I stood there wondering, fearing, doubting, dreaming dreams no mortal ever dared to dream before...

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Offlinerev2010
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Re: agar question [Re: CapnZ]
    #24145605 - 03/08/17 06:39 AM (7 years, 10 months ago)

I did 15 psi for 30 min. Not sure the temp. Fuckin hot. Lol I let it cool slowly. Did not try relieve pressure from PC

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Offlinerev2010
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Re: agar question [Re: rev2010]
    #24145612 - 03/08/17 06:43 AM (7 years, 10 months ago)

also i have been reading alot of different things about agar. im doing b+. i took a print from the biggest fruit i had in my mono tub. i have been reading its better to take from the little fruits? is that true?

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OfflineCapnZ
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Re: agar question [Re: rev2010]
    #24145613 - 03/08/17 06:44 AM (7 years, 10 months ago)

Good let it cool slowly. Yup some sediment is usual. Swirl it around when it's still decently hot and it should go back in suspension. Pour when your agar gets at or just below 120 F.


--------------------
Deep into the darkness peering, long I stood there wondering, fearing, doubting, dreaming dreams no mortal ever dared to dream before...

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Offlinemushpunx
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Re: agar question [Re: rev2010]
    #24145660 - 03/08/17 07:30 AM (7 years, 10 months ago)

Quote:

rev2010 said:
also i have been reading alot of different things about agar. im doing b+. i took a print from the biggest fruit i had in my mono tub. i have been reading its better to take from the little fruits? is that true?




No, it doesn't matter what fruit you take the spores from spores are spores.

When you pour, wait until it is cool to the point it might start to solidify if you let it go any longer.
This will prevent condensation in the plates , its the best trick I learned. Before I figured that out I always had condensation on the top couple plates, would have to set a mug of hot water on top of the stack as it cooled to stop it.


--------------------

Amateur Mycologists United
AMU Q&A

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InvisibleLeftfield420
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Re: agar question [Re: rev2010]
    #24145695 - 03/08/17 07:46 AM (7 years, 10 months ago)

Sounds like you let it cool too much...I've had that happen... Pasty's easy agar tek is a little easier than pouring plates... Either way in my experience takes about the same time...I have glass Petri's and cleaning them can be a pain in the ass...then not to mention oven sterilizing them...think I'm going back to pasty plates very soon actually

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OfflineNextStopWilloughby
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Re: agar question [Re: rev2010]
    #24145851 - 03/08/17 09:22 AM (7 years, 10 months ago)

Quote:

rev2010 said:
also i have been reading alot of different things about agar. im doing b+. i took a print from the biggest fruit i had in my mono tub. i have been reading its better to take from the little fruits? is that true?




I've heard that about taking a clone sample- reasoning being there is still a lot of "young" energy in the mycelium, much like transferring from the edge of growth on a plate. There are two problems with this theory. 1st is smaller fruits are harder to get a clean culture from, the second is I've never seen any data to back that theory up. As far as spores go it does not matter which caps you print- other than the size of print you want.

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Offlinerev2010
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Re: agar question [Re: rev2010]
    #24239824 - 04/13/17 09:26 AM (7 years, 9 months ago)

can someone help me out. i dont have a picture at the moment, but when i am doing spores from prints on agar the mecelium is very light. like transparent or what ever. what do i need to do different. i have put some of this agar to wbs and it grows out just fine, just curious as to why its see through and not white. once i put it in wbs it grows out a very briliant white. thanks

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