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InvisibleHighzenbergfungi
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B+PE Grow Log
    #24137503 - 03/04/17 11:42 PM (7 years, 10 months ago)

I'm getting this started a little early

we have a few more steps to go until we find out if all our efforts were in vain or not.


may have gone a little overboard on room air scrubbing but i felt the need for one of these anyways. helps with the allergies. we have a few animals here.. all the scrubbing we can get in my eyes. other air scrubbing in the room. hepa air inlet with 300cfm behind it. an additional hepa inlet in case the room becomes too warm. this also helps keep positive pressure in the room when the door is opened. heater and secondary cool air inlet are on a thermostat. able to stabilize the room temperature within a degree according to the thermostat...when no one is going in and out of the room.


Using these bulbs. 2 tubs per bulb. 6500k, 65 watts, 3000 lumens. the tubs will be turned around throughout the day when FAE and misting is needed.


i placed a few of these around the room to check the temperature in the surrounding area and the temperature of the tub affected by the substrate inside.


Liquid culture jars were made up of 500ml of Water and a pre determined amount of Raw Honey.

they were sterilized at 15 psi for 20 minutes and allowed to cool over night.

the following day they were inoculated with multi spore syringes. one of B+ and one of PE

they were allowed 10 days to fully grow out in a tub incubator. this incubator consisted of a plastic tub surrounded by another plastic tub filled with water and a water heater set to 80f

every other day the jars were "swirled" to aid the break up of material.

after 10 days, 2 Liquid Culture jars were inoculated from the PE multi spore LC and 6 jars were inoculated from the B+ multi spore LC jar.

these were allowed another 7 days to fully grow out, every day they were broken up by "swirling" the material


7 days after transfer to second jar


when these were ready

a pre determined amount of rye grain spawn bags were sterilized.

5-10CC of LC was added to each Spawn Bag

7 days later




during colonizing the bags were broken up twice, after a significant amount of mycelium had grown out in a certain section the bag was massaged until the infected kernels were mixed in with the unaffected areas.

a secondary batch was done like this except the bag was massaged immediately after inoculation. this seemed to have better results at completely colonizing the bags sooner

the bags seemed to like an ambient temperature of 78f. 80f and 75f caused them to seemingly slow down. 

the B+ bags had a more rhizomorphic characteristic while the PE had a mix of both rhizo and cotton look. The B+ colonized faster then the PE in all temperatures. More bags were ready to be added to substrate of the B+ then the PE

4 bags of Spawn were added to 10 lbs of substrate in a 56 quart tub. tub was pre disinfected with Lysol and lined with a black trash bag.
Substrate consisted of Horse Poo, "exotic zoo poo", Organic Coconut Coir, Vermiculite, and gypsum.
grain was broken up and tossed very thoroughly in the substrate, it was not packed down. bag was closed up gently and a good amount of air was pushed out of it. the bag was loosely tied and the tub was closed.


Because both strains have proved to be a bit slower then "projected" times of process finishing we have decided to wait 12 days before checking for full colonization

tubs will be cased with verm/peat moss with lime water ph'd to 6.8

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Offlinemushpunx
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Re: B+PE Grow Log [Re: Highzenbergfungi]
    #24137898 - 03/05/17 06:28 AM (7 years, 10 months ago)

3 things see right off the bat.

1. Never inoculate LC with spore syringes. Never. Its not clean enough. It needs to be inoculated with a clean culture on agar. There are a ton of spore to LC teks but they are all bad info. Wait until you have more experience before using LC.
For now, if you don't want to Learn agar just inoculate with 1 drop of spore.
Did you at least test the LC before you used it?

2. 80° is too warm. Don't use the incubator,colonize at room temp unless your room temp is below the sixties F

3. How did you pasturize your substrate? H Poo needs to be properly pasteurized, kept between 140 and 170°F for one hour-


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OfflineBrown Buffalo
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Re: B+PE Grow Log [Re: mushpunx]
    #24137911 - 03/05/17 06:34 AM (7 years, 10 months ago)

Nice bags, what kind of bags are those?

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Offlinemushpunx
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Re: B+PE Grow Log [Re: Brown Buffalo]
    #24137925 - 03/05/17 06:39 AM (7 years, 10 months ago)

Those look like the premade bags to me but maybe not

You can make your own infection ports on spawn bags by putting silicone on the inside and out and squeezing it between a couple coins

Although they are pretty unnecessary
You can just inject thru the bag and cover with tape


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InvisiblePipefitter537
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Re: B+PE Grow Log [Re: mushpunx] * 1
    #24138025 - 03/05/17 07:54 AM (7 years, 10 months ago)

Quote:

mushpunx said:


You can make your own infection ports





Intentional or accidental?  Right on if you meant it


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Offlinemushpunx
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Re: B+PE Grow Log [Re: Pipefitter537] * 1
    #24138028 - 03/05/17 08:02 AM (7 years, 10 months ago)

Ahahahaha

No accidental. My phone's autocorrect


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InvisibleHighzenbergfungi
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Re: B+PE Grow Log [Re: Brown Buffalo]
    #24138283 - 03/05/17 10:28 AM (7 years, 10 months ago)

Quote:

mushpunx said:
3 things see right off the bat.

1. Never inoculate LC with spore syringes. Never. Its not clean enough. It needs to be inoculated with a clean culture on agar. There are a ton of spore to LC teks but they are all bad info. Wait until you have more experience before using LC.
For now, if you don't want to Learn agar just inoculate with 1 drop of spore.
Did you at least test the LC before you used it?

2. 80° is too warm. Don't use the incubator,colonize at room temp unless your room temp is below the sixties F

3. How did you pasturize your substrate? H Poo needs to be properly pasteurized, kept between 140 and 170°F for one hour-





Thanks for the advice, we were left with the option of LC, for simple time constraint reasons. We inoculated our multispore jar with very little. To be honest it may have been 1 drop. Our needle was properly heat treated before each injection.

Thus far I still have 31 bags (we did ...a few) 31 bags still colonizing. For the simple fact that we did not have enough substrate and we expected contaminations. So far of those we have had zero obvious signs of contamination. In fact, none of the bags we did showed any sign of contamination.

The substrate was prepared for us by a reputable source. We'll know for sure in a few days from now when we birth our tubs.

Quote:

Brown Buffalo said:
Nice bags, what kind of bags are those?




They are the pre made solid injection port, good air exchange, relatively cheap.


Although we made our ports for our LC jars, I did not feel comfortable making the injection ports on this type of plastic.. saw these and bit the bullet.

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InvisibleHighzenbergfungi
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Re: B+PE Grow Log [Re: Highzenbergfungi]
    #24138365 - 03/05/17 10:53 AM (7 years, 10 months ago)

Maybe beginners luck :stoned:

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InvisibleHighzenbergfungi
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Re: B+PE Grow Log [Re: Highzenbergfungi]
    #24138442 - 03/05/17 11:19 AM (7 years, 10 months ago)

Also the room temperature would be 50-60f without supplemental heating.

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InvisiblebodhisattaMDiscordReddit
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Re: B+PE Grow Log [Re: Highzenbergfungi]
    #24138457 - 03/05/17 11:25 AM (7 years, 10 months ago)

Heat the room to room temperature then.

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Offlinemushpunx
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Re: B+PE Grow Logh [Re: bodhisatta]
    #24138627 - 03/05/17 12:33 PM (7 years, 10 months ago)

:whathesaid:

Well I hope you get lucky! I'm really not trying to sound discouraging man


Unfortunately most of the time contamination doesn't present itself in spawn. Just about every substrate I have ever lost to mold the spawn looked and smelled Clean. Its not until its fruited, and the contamination is exposed to fruiting conditions does it sporulate


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Edited by mushpunx (03/05/17 12:37 PM)

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InvisibleHighzenbergfungi
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Re: B+PE Grow Logh [Re: mushpunx]
    #24139828 - 03/05/17 08:44 PM (7 years, 10 months ago)

Well, that worries me a little but I'll pray to the gods of the mycelium bound fruits that the next few days go well.

I'll update as things come to pass

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InvisibleHighzenbergfungi
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Re: B+PE Grow Logh [Re: Highzenbergfungi]
    #24149502 - 03/09/17 05:58 PM (7 years, 10 months ago)

New update, no pictures yet sadly :crazy:

We opened a few tubs up and peaked to find a bit of a sour smell and that the mycelium hadn't fully colonized yet. So far it's been 15 days. They're close but I think part of the issue was we did not use enough spawn Per tub. I'm relatively new to all this and have been relying on someone with a lot more experience. After seeing the spawn bags colonize a bit slower then was projected I was worried the tubs may take longer, then when we realized we did not have enough spawn to really give the tubs a good mix I was again worried.

We brought the temp down to 75f a couple days ago when you guys suggested. And then further down to 72f yesterday as I mixed the next tubs up. I believe we're still not using enough spawn bags per tub. Currently we're using 5 lbs spawn to 10lbs substrate.

I believe the next batch of spawn we are going to skip horse poo compost, one reason is where we live drying it is very difficult. Even though it is in abundance in the area.. drying is too difficult. So we will try our luck with coir

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InvisibleHighzenbergfungi
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Re: B+PE Grow Logh [Re: Highzenbergfungi]
    #24176677 - 03/20/17 02:47 AM (7 years, 9 months ago)

We've come to the conclusion that our incubation temps for the tubs was too high at 80f

As well as both the substrate we did and spawn bags were too low in moisture content from the get go.. severely stunting the growth..

I know this as I've had many totes to experiment with since the failure.

The ones I misted daily in the trash Bags have recovered, very rapidly and seem to be throwing up threads of rizomorphic growths in all directions hunting for this new water


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OfflineCapnZ
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Re: B+PE Grow Logh [Re: Highzenbergfungi]
    #24176860 - 03/20/17 06:27 AM (7 years, 9 months ago)

Do you still notice a sour smell that you noted previously? From the looks of that last pic you should be smelling fresh mushrooms, nothing sour or sickly sweet. Sour smell is a comcern. I keep my area right at 70  F.....


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Deep into the darkness peering, long I stood there wondering, fearing, doubting, dreaming dreams no mortal ever dared to dream before...

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OfflineCapnZ
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Re: B+PE Grow Logh [Re: CapnZ]
    #24176861 - 03/20/17 06:29 AM (7 years, 9 months ago)

Also your substrate surface in that last pic looks really uneven. That allows moisture / water to pool up which you don't want.


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Deep into the darkness peering, long I stood there wondering, fearing, doubting, dreaming dreams no mortal ever dared to dream before...

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InvisibleHighzenbergfungi
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Re: B+PE Grow Logh [Re: CapnZ]
    #24178651 - 03/20/17 06:48 PM (7 years, 9 months ago)

Smell has been very good. Like button mushrooms. We threw out most of the sour ones. The ones I experimented with adding moisture to recovered and don't smell anymore.

I'll try to make it more even next time. I was told the rolls were okay.

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InvisibleHighzenbergfungi
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Re: B+PE Grow Logh [Re: Highzenbergfungi]
    #24186845 - 03/23/17 05:54 PM (7 years, 9 months ago)

So far, all monotubs I experimented by listing instead of chucking because I had expected contam are recovering nicely. A few had fully colonized the top so we put them in fruiting conditions. Last night we added super saturated verm for a casing layer to 2 of them.
I came in to check this afternoon and the mycelium had already reached the surface in some spots.. it's seems we've created a water hungry monster after they were all starved for water for this long. Our spawn was dry and our sub was dry.. and we couldn't for the life of us figure out why everything was moving so slow.

All the tubs have begun to shrink which is unusual because none have fruited yet. But it seems that we may be a little bit before we fruit due to the need for moistures of these tubs. We'll be casing the rest of them today.

Used about a half inch of casing. Just something we can constantly mist to get the moisture to enough so they fruit. We were tempted to dunk them just to try and help but seeing as how some of them have cracked due to them drying up we decided it may not be a good idea.



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InvisibleHighzenbergfungi
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Re: B+PE Grow Logh [Re: Highzenbergfungi]
    #24187354 - 03/23/17 09:13 PM (7 years, 9 months ago)

3 quarts verm to 3 cups water were put in a microwave for 6 minutes and then the fridge until cool. Turkey oven bags were used to contain all of this.

This was the amount we used to case each tub

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InvisibleHighzenbergfungi
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Re: B+PE Grow Logh [Re: Highzenbergfungi]
    #24192424 - 03/25/17 04:15 PM (7 years, 9 months ago)

Got our first pins today from a batch we threw into a tub on the 28th of last month

Yaaay!
I checked everything last night and there were no pins. Woke up today to find these little guys sticking up out of the verm.



They're already about an inch tall. Pretty quick for 6 hours of time..

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Re: B+PE Grow Logh [Re: Highzenbergfungi]
    #24192431 - 03/25/17 04:18 PM (7 years, 9 months ago)

:rockon:

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InvisibleHighzenbergfungi
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Re: B+PE Grow Logh [Re: Psilosoulful]
    #24198816 - 03/28/17 03:30 AM (7 years, 9 months ago)


Tried to do a photo every 6-12 hours until the first veil popped. The next few photos will see a few missing as they keep coming up.

We're not getting a single flush with these, more like a constant growing cycle.




Saw this cute little guy with a fuzz hat on

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InvisibleHighzenbergfungi
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Re: B+PE Grow Logh [Re: Highzenbergfungi]
    #24201133 - 03/28/17 09:34 PM (7 years, 9 months ago)

So far harvest is going along smoothly.
We stopped misting the other tubs we have and they all have thrown up small threads, most likely to check humidity coming in since we stopped.. they will most likely fruit soon

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InvisibleHighzenbergfungi
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Re: B+PE Grow Logh [Re: Highzenbergfungi]
    #24201451 - 03/28/17 11:55 PM (7 years, 9 months ago)



Some of the first "flush" harvest. We mutilated a few others for their guts (agar transfer for genetic selectivity)

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InvisibleHighzenbergfungi
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Re: B+PE Grow Logh [Re: Highzenbergfungi]
    #24218662 - 04/04/17 06:19 PM (7 years, 9 months ago)



Ended up with this beautiful monster cluster.
All this is originally from MS so we've actually seen a bunch of different expressions in the genetics.

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InvisibleHighzenbergfungi
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Re: B+PE Grow Logh [Re: Highzenbergfungi]
    #24240353 - 04/13/17 12:32 PM (7 years, 9 months ago)

We didn't end up with very consistent flushes using only verm as a casing. It did hold the moisture level very high but to the point of unnecessary. We are going to try the wax paper tek the day of full colonization on the next round. No PE tubs ended up making it to fruiting. All had issues colonizing. Substrate was indeed too dry.


A little art for ya


Ended up with this monster cluster, weighing in at 187g wet


Part of said cluster. 47g wet

Tissue samples from this cluster in a tub glove box, onto agar dishes.
Took 2 samples from the medium size fruit and 3 from the largest.

Wasn't sure which was the best to take from so I figured well fruit all that make it out of those samples and see what we get.

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Invisiblemynakedrat
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Re: B+PE Grow Logh [Re: Highzenbergfungi]
    #24241916 - 04/13/17 10:20 PM (7 years, 9 months ago)

Wow man. That monster cluster is just.
Wow
So beautiful.

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InvisibleHighzenbergfungi
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Re: B+PE Grow Logh [Re: mynakedrat]
    #24267651 - 04/24/17 01:24 AM (7 years, 8 months ago)



Some monsters from a few flushes. Our room seems pretty decent right now. A few of our tubs are on their 4th flush going strong.

Though the wax paper tek didn't seem to do as expected for the pin set. Not sure if we did it wrong.

Basically after full colonization we added our polyfill and a sheet of wax paper. Didn't open it for 4 days. The new coir+verm 50/50 colonized very quickly. 4 quarts substrate to 4 spawn bags..

Colonized in 5-6 days. Didn't open it til 7 days to allow consolidation. Wax papered it.. no pins after 5 days.. we removed the wax papers. The tubs began to pin but not evenly.. could just be the MS we're using.

Edited by Highzenbergfungi (04/24/17 01:30 AM)

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