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Invisiblebaltazar
Quiet dreamer

Registered: 02/23/04
Posts: 746
spore print -> spore syringe question
    #2413091 - 03/10/04 01:13 AM (13 years, 6 months ago)

Hello ... i wanted to ask you guys from experience how much spore solution do you make from one spore print ? i was thinking something like 30-40 ml distilled water to one print ??? what do you think about that ?

And i have jars with 5 holes on the lid ( 4 on the edges , 1 in the center ) ... How much solution should i put in each ? 0.5 ml ???

Can i use a diabetic injection needle for sucking up the spores ?


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InvisibleYarry
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Registered: 01/05/04
Posts: 23,740
Re: spore print -> spore syringe question [Re: baltazar]
    #2413452 - 03/10/04 03:10 AM (13 years, 6 months ago)

Depends on how big your print is dude, you can usually make 5 or so syringes outta one print and thats if theyre dark. I wouldnt use your whole print at once tho, just scrape off what you need into sterile water.

I usually put 0.25cc or so into each whole in my jars and they do fine.

Im sure that using a diabetic needle wouldnt be a problem just make sure its sterile


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InvisibleMagashM
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Registered: 07/25/02
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Re: spore print -> spore syringe question [Re: baltazar]
    #2413681 - 03/10/04 06:26 AM (13 years, 6 months ago)


Bring some water to boil on the stove and let it boil about 10 minutes.
Suck the boiling water up to a fresh syringe.
If the syringe was used before, sterilize the allready filled syringe in a PC for 15 minutes.
Put syringe aside until cool.
Following tasks should be idealy prepared in front of a flow hood, but a reasonably cleaned bathroom will do:
Prepare the print, a shot glas and the alcohol lamp.
Rinse the clean shotglass with isopropanol or rubbing alcohol and let it evaporate a few seconds.
Bring the needle of the syringe to red glow above the alcohol lamp.
Squirt 1/2 ml of water out of the syringe to cool the needle and create some airspace in the syringe so later you can shake the spores.
Shake of the eventual drop on top of the needle off so the print won?t become wet.
Scrape some spores with the tip of the needle into the shotglas.
Add a small amount of water from the syringe, stir and suck it back into syringe.
Give the syringe a vigorous shake, let the spores rehydrate for a few hours and inoculate

Diabetic sringes suck. They clog to easy. I thought I could save money by using some of mine and I found it a pain.


--------------------
All creatures tremble when faced with violence. All creatures fear death, all love life. If we can only see ourselves in others, then how could we possibly hurt another creature?


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Invisiblebaltazar
Quiet dreamer

Registered: 02/23/04
Posts: 746
Re: spore print -> spore syringe question [Re: Magash]
    #2418259 - 03/11/04 08:22 AM (13 years, 6 months ago)

YAAY i gote two print's today ... ecuadorian strain ... the spore print's are 5 cm wide ... ill make 2 syringes later today ...


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Invisiblebaltazar
Quiet dreamer

Registered: 02/23/04
Posts: 746
Re: spore print -> spore syringe question [Re: baltazar]
    #2418415 - 03/11/04 09:42 AM (13 years, 6 months ago)

some problems ... i used half of spore print for 15 ml spore solution ... Now i see that the solutions isn't much darker than normal water ... Is this ok ? I also have spore klumps in the solution that don't wan't do disolve .... Please help ...


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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