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OfflineTira
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Registered: 11/20/10
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Some agar plates, what do you think? (Galindoii, PEU, PanCyan Hawaii, Golden Teacher)
    #24081336 - 02/11/17 03:18 AM (7 years, 12 days ago)

Some time ago, I inoculated some small jars of wheat with Psilocybe Galindoii, Penis Envy Uncut and Pan Cyan Hawaii syringes as I was having a hard time getting them to germinate directly on agar.

I then took some grains from those jars and put them on agar plates and transferred as soon as I observed some growth.  Now I'm trying to get some clean cultures and start some grows.  Here are the plates:

Psilocybe Galindoii


That white liquid on the first one is bacteria, right?  What do you think about the others?  Do they look clean, look like galindoii myc to you?

These are first transfers from the original plates.



and this is what I did with the leftover colonized grains (at least the ones looked/smelled cleaner than the others) after I transferred some to agar plates; I sprinkled some sterilized coir&verm in there and shook.  It colonized really fast but I can't be sure if it's galindoii myc as I don't have any experience with it.




Psilocybe Cubensis PEU


These are also first transfers from the original plates.

Again, the last one looks contaminated heavily with bacteria.  Some of the others have lots of tiny points of growth as a result of me sometimes having a hard time getting the agar wedge off the scalpel while transferring .) But 5th one looks pretty promising to me, let me know what you think.



Small wheat jars of PEU looked really clean, so I spawned them to a mini-tub, using coir&verm as substrate. it's almost completely colonized now.



and this is a small project just for fun.  I mixed the colonized parts of the original plates (after I made the transfer) and mixed them with some sterilized coir&verm.




Panaeolus Cyanescens Hawaii


I had a really hard time getting these to start on agar, as the syringe was heavily contaminated with bacteria. As you see, the jar of grains is leaking metabolites, and only the quarter of it is colonized (and this is the best looking jar of 5 jars I inoculated)



These are all original plates that I inoculated with grains from the jars.  I did some transfers from the first two about 6 days ago, I'll upload the pics of those plates when they show significant grow.




I'm not sure what's up with the last plate, I kind of had a hard time getting the grain off the scalpel there too, but I'm not sure if that is what caused growth on that side and I'm not sure if it's eve Pan Cyan myc.





Psilocybe Cubensis Golden Teacher


I also have these going on, I started this culture from a fruit from a grow I did about 2 months ago.  I cloned that fruit and forgot about the plate for about 2 months.  As I transferred grains from jars, I also did a transfer from that plate.

These are second transfers from the original plate.



I know the pics aren't great but condensation makes it really hard to take good pics sometimes.  Hope you'll like these.  Let me know what you think, and let me know if you have any advices .) Thank you for reading.


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Edited by Tira (02/13/17 12:22 PM)


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Invisibleblindingleaf
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Re: Some agar plates, what do you think? [Re: Tira]
    #24081370 - 02/11/17 04:09 AM (7 years, 12 days ago)

thats a lot of pictures :crazy:

ill just tackle a couple, someone else can sort thru the rest :lol:

that second galadoi I would put to grain.

I agree on the 5th PEU>grain

the 2nd pan cyan Hawaii plate looks ok, I would still transfer 1 more time tho.  the 4th pic on the side I think is a contaminant, but its a little blurry.

cool ur scalpel for longer before touching the mycelium.  it will help prevent sticking.  I dip it in the receiver plate, or sometimes even the donor plate if its clean and there is space.  I hold it there till I don't hear a sizzle, 2-3 seconds.

all around looking good tho:cool::thumbup::mushroom2:


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OfflineTira
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Re: Some agar plates, what do you think? [Re: blindingleaf]
    #24081895 - 02/11/17 10:23 AM (7 years, 12 days ago)

I'm actually thinking of transferring all of them one more time.

Quote:

blindingleaf said:

cool ur scalpel for longer before touching the mycelium.  it will help prevent sticking.  I dip it in the receiver plate, or sometimes even the donor plate if its clean and there is space.  I hold it there till I don't hear a sizzle, 2-3 seconds.





I actually do that; dipping the scalpel in the receiver plate.  But sometimes I stick the scalpel too deep into the wedge and it becomes difficult to get it off it.
Quote:

blindingleaf said:

all around looking good tho:cool::thumbup::mushroom2:




thank you .)


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OfflineTira
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Re: Some agar plates, what do you think? [Re: Tira]
    #24082345 - 02/11/17 02:12 PM (7 years, 12 days ago)

Does anyone have any opinions on those bacterial-looking plates?  Are those really bacteria?



Oh, and here is a Golden Teacher I grew on a piece of paper towel, used a ziploc bag with holes on it as a FC.



Just wanted to share .) Love how this stuff just wants to live, I just poured some water on a folded paper towel, put about 4-5 grains in it, and folded it and placed it in the ziploc bag.


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OfflineTiamo
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Re: Some agar plates, what do you think? [Re: Tira]
    #24082356 - 02/11/17 02:18 PM (7 years, 12 days ago)

:eek: That's amazing :lol:


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OfflineTira
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Re: Some agar plates, what do you think? [Re: Tiamo]
    #24084160 - 02/12/17 09:42 AM (7 years, 11 days ago)

Plates containing the first transfers made from the original Pan Cyan plates started showing significant grow.

Should I make a transfer ASAP or wait a bit and then do it?



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OfflineTira
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Re: Some agar plates, what do you think? [Re: Tira]
    #24087053 - 02/13/17 11:21 AM (7 years, 10 days ago)

I am going to make transfers from all strains in a few hours.  Would really appreciate some feedback on the plates .)


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OfflinePanarchist
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Re: Some agar plates, what do you think? [Re: Tira]
    #24088213 - 02/13/17 08:09 PM (7 years, 9 days ago)

this one looks pretty good from any where between 3:00 and 9:00.



these i wouldn't bother with.



this one looks the nicest. i would think anywhere between 7:00 and 2:00 would be good.





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InvisibleMad Season
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Re: Some agar plates, what do you think? [Re: Panarchist]
    #24088316 - 02/13/17 08:55 PM (7 years, 9 days ago)

Galindoi- 2nd and last look the best to me. The shit in the middle kinda looks like what happens when there's water on the surface as it colonizes through it. The grain jars look like galindoi to me.

PEU- so many satellites :frown: 5th is the only one I'd do anything with.

Pan cyan receny transfers look good, I agree with pan, you can wait a bit or xfer now. You're call really.

Gt- 1st is the only one I'd trust anything from.


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OfflineTira
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Re: Some agar plates, what do you think? [Re: Mad Season]
    #24088664 - 02/13/17 11:25 PM (7 years, 9 days ago)

Quote:

Mad Season said:
Galindoi- 2nd and last look the best to me. The shit in the middle kinda looks like what happens when there's water on the surface as it colonizes through it. The grain jars look like galindoi to me.

PEU- so many satellites :frown: 5th is the only one I'd do anything with.

Pan cyan receny transfers look good, I agree with pan, you can wait a bit or xfer now. You're call really.

Gt- 1st is the only one I'd trust anything from.




Exactly the ones I chose .) thank you for confirming.  Did the transfers, will post more as they grow and hopefully this time I'll use them to inoculate stuff.


The jar actually does smell like cucumbers so I guess it is galindoii, so weird something that puffy is not contamination lol


Is there anything I should know about fruiting galindoii, or is it just like fruiting cubes?


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InvisibleMad Season
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Re: Some agar plates, what do you think? [Re: Tira]
    #24088680 - 02/13/17 11:32 PM (7 years, 9 days ago)

It's as easy as cubes :wink: just don't stuff the holes as tight for more FAE, and I'd probably case too.


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OfflineTira
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Re: Some agar plates, what do you think? [Re: Mad Season]
    #24088878 - 02/14/17 02:12 AM (7 years, 9 days ago)

Quote:

Mad Season said:
It's as easy as cubes :wink: just don't stuff the holes as tight for more FAE, and I'd probably case too.




Should I case with straight verm as I'm having a hard time finding peat moss around here?


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InvisibleMad Season
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Re: Some agar plates, what do you think? [Re: Tira]
    #24089426 - 02/14/17 09:47 AM (7 years, 9 days ago)

Could even do a CVG casing :thumbup:


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OfflineTira
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Re: Some agar plates, what do you think? [Re: Mad Season]
    #24120775 - 02/26/17 09:15 AM (6 years, 11 months ago)

Got some updates .) These are third transfers from the original Golden Teacher clone plate.  I'm planning on dropping some wedges to grain jars I'm PCing right now



and this is the current state of the second transfer plate I took the wedges to inoculate the plates above



Is it normal that they look extremely stringy like this?  Which one would you prefer to use?


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OfflineTira
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Re: Some agar plates, what do you think? [Re: Tira]
    #24127826 - 03/01/17 06:35 AM (6 years, 11 months ago)

Last night I inoculated some grain jars with these plates,

they are 3rd transfers from a PEU MS plate



I wonder why the growth is thinner in some areas, could water standing on agar cause that?


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