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Throwaway
The wizard



Registered: 04/22/21
Posts: 805
Last seen: 3 days, 13 hours
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Quote:
lifescientist said: How do I open the jars in the pressure cooker? Stepwise?
1. Clean inside of SAB with alcohol 2. Wipe top of jars with alcohol wipe 3. Turn flame on 4. Open jars (but some of them are impossible to open...?)
5. Have plates ready to go 6. Flame sterilize blade in-between every cut 7. Close jars 8. Shake jars 9. Hope for the best?
I think you can find a lot of resources on how to use your SAB properly for A2G. A big tip that made it a lot easier for me is to think out every step before doing your SAB work. So mine would look like this for one jar (I don't like dry SAB work before anyone asks): 1. Wipe down SAB with soapy water (using alcohol is a waste) 2. Wipe agar plate and jar (I use alcohol here) 3. Place stuff in SAB 4. Place torch, parafilm strip & scalpel infront of SAB. 5. Quick spritz SAB inside with soapy water 6. Cover arm holes with paper and go watch an episode of your favorite show
7. Carefully remove arm hole covers 8. Wet up my arms/hands with soapy water 9. Unwrap petri and loosen jar lid 10. Arms out, grab scalpel and flame it 11. Put in arms
Now this is where swiftness is important: 12. With left I grab the petri cover/top, quickly select and cut a piece of agar, pierce it on the scalpel, close the dish. 13. Move scalpel to jar, open lid, drop it in there or if it's small just drag it against the edge to make it let go, close the jar with one hand semi tightly. 14. Lay down scalpel, close jar properly 15. Arms out, grab parafilm/clingwrap 16. Arms in, and wrap up your dish
Edited by Throwaway (04/13/23 05:29 AM)
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lifescientist
Stranger
Registered: 10/31/17
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Re: BOD's Easy AF OAT prep tek. [Re: Throwaway]
#28276299 - 04/13/23 06:15 PM (9 months, 10 days ago) |
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Ok. Thanks. I followed that advice. Unfortunately my blade almost fell off so I ended up having to touch it pre flame sterilization to tighten it up. One of the lids, I screwed on tight but I think I messed up the SFD or something. I am gonna hope that everything goes well, this was pretty hard lol (physically and thought wise)
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socalchef00
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Registered: 06/09/23
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Re: BOD's Easy AF OAT prep tek. [Re: bodhisatta]
#28354337 - 06/10/23 10:22 AM (7 months, 14 days ago) |
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This is awesome. Only ever used BRF cakes. Are. grains better?
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Mycelionaut
In Orbit



Registered: 06/20/20
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Re: BOD's Easy AF OAT prep tek. [Re: socalchef00]
#28355447 - 06/11/23 10:45 AM (7 months, 13 days ago) |
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How long would sterilized grain jars typically last before inoculating? If one made a whole slew of oat jars, is there a rule of thumb for how long they can stay put before adding agar wedges? I try to do it as soon as possible but was just wondering in case my agar needed a few more transfers than expected, would the jars hold for a few weeks, months, etc…hypothetical scenario.
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ApeDork
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Re: BOD's Easy AF OAT prep tek. [Re: Mycelionaut]
#28381732 - 07/01/23 07:04 PM (6 months, 24 days ago) |
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Thank you. Just did my first 4 jars of oats, 3 with spores and one with agar (first ever).
thanks again for the good write up.
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dirtytortle

Registered: 12/06/18
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Re: BOD's Easy AF OAT prep tek. [Re: ApeDork]
#28381753 - 07/01/23 07:26 PM (6 months, 24 days ago) |
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This is my favorite grain prep tek. Truly easy AF and easy on the wallet too!
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scrubbindavie
Stranger


Registered: 07/10/23
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Re: BOD's Easy AF OAT prep tek. [Re: bodhisatta]
#28420553 - 08/04/23 01:49 PM (5 months, 21 days ago) |
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so, I went ahead and purchased some whole oats, and have been doing really well with my liquid culture so I have plenty of inoculum. Have cooked oats twice and not found any white mycelium after inoculation. Am I being impatient? The culture grows like wild fire in lc container... followed Bod's tek to the T. Help lmk what I'm doing wrong. Also live in Colorado, and wonder if I may need to cook longer for altitude??? Heeeeelpp please!!!
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PreparationH
apply daily

Registered: 03/28/05
Posts: 18,306
Loc: Amsterdam
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How long since you've inoculated?
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scrubbindavie
Stranger


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Re: BOD's Easy AF OAT prep tek. [Re: PreparationH] 1
#28421922 - 08/05/23 02:17 PM (5 months, 20 days ago) |
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Actually had been about 4 days, and was just beginning to think it was my brand of oats, but checked jars on lunch break and finally got some starting to colonize! Thank God!
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PreparationH
apply daily

Registered: 03/28/05
Posts: 18,306
Loc: Amsterdam
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Glad to hear, good luck
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Shroosier
Stranger

Registered: 05/27/23
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Re: BOD's Easy AF OAT prep tek. [Re: bodhisatta]
#28446180 - 08/25/23 08:17 AM (5 months, 21 hours ago) |
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I followed this prep to the letter, even let the oats sit in the fridge overnight. I inoculated several jars with agar and none of them have been colonizing well at all. I see a little growth directly around the agar but that’s it. Any ideas on what I may have done wrong?
At the time of these pictures the agar has been in the jar 2 weeks:


Edited by Shroosier (08/25/23 09:14 AM)
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Screwup
Googles your dumb questions


Registered: 01/27/22
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Re: BOD's Easy AF OAT prep tek. [Re: Shroosier]
#28446181 - 08/25/23 08:19 AM (5 months, 21 hours ago) |
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What filter are you using? Have you shaken em? Pics of agar etc etc
-------------------- 🅃 🄴 🄰 🄼 🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿 Help US help YOU TEK 2023 Dehydrator TEK
Edited by Screwup (08/25/23 08:21 AM)
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Shroosier
Stranger

Registered: 05/27/23
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Re: BOD's Easy AF OAT prep tek. [Re: Screwup]
#28446222 - 08/25/23 09:10 AM (5 months, 20 hours ago) |
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I put in condiment cups of agar that had been completely colonized and cut into about 6 wedges. I then shook the grain jars to distribute the agar pieces throughout and haven’t shaken them since. The filters are 3m micropore tape, applied on the top and bottom of the lid with a hole a little smaller than a dime in them.
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Huskies
Boop More Snoots



Registered: 03/22/16
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Re: BOD's Easy AF OAT prep tek. [Re: Shroosier]
#28446321 - 08/25/23 11:27 AM (5 months, 18 hours ago) |
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Agar can be slower since it has less inoculation points. GRowth seems good, I'd give it a shake to spread out the mycelium towards the rest of it jar. If it doesn't recover, then perhaps it was fighting bacteria/mold.
-------------------- I call them Huskies cause you tell them to go "Mush! Mush""
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Kinoko314
Stranger Danger



Registered: 12/16/22
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Re: BOD's Easy AF OAT prep tek. [Re: Shroosier] 1
#28446324 - 08/25/23 11:32 AM (5 months, 17 hours ago) |
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That should allow plenty GE. It's possible that it's the brand of oats. Sometimes they use anti-fungal stuff to protect the grain, so that could be causing issues.
I've been using oats, and I had different issues, but since I started washing the grain first I have had better results. You could give that a try.
If it's not the grain then it would probably have to be that your agar was contaminated in the first place. Pics of agar would help.
Bod says it can't get too dry, but it seemed to me that if the grain was all bone dry (on the outside), it would take longer to colonize. Your situation is a bit more extreme though.
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phungiphreak
Propagater


Registered: 07/06/23
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Re: BOD's Easy AF OAT prep tek. [Re: Kinoko314]
#28456352 - 09/02/23 07:00 PM (4 months, 23 days ago) |
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I have seen this with oats. Well not necessarily no growth, but jars that refused to recover after a good shake. Its been the case with mine that the oats in the jars weren't quite properly hydrated as much as they needed to be. All that can be done is to start over. I would personally use liquid culture inoculate the jars and use your agar to make the liquid culture. Just a suggestion.
Edited by phungiphreak (09/02/23 07:01 PM)
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Shroosier
Stranger

Registered: 05/27/23
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Re: BOD's Easy AF OAT prep tek. [Re: Shroosier]
#28465299 - 09/11/23 11:34 AM (4 months, 14 days ago) |
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Still struggling with how long colonization is taking with this TEK and not sure what I'm doing wrong. I redid another batch, boiled until I saw burst oats, also cut some in half to verify there was no white inside. Then I drained them, spread them out on two cookie sheets, and let them sit overnight in my fridge to dry out.
I've inoculated them 2 ways, both which seem to be slow going.
1: Straight from a LC syringe that I purchase online. Here, the area the LC was squirted seems to have inoculated nicely, but it just isn't spreading out from there at all.
This is 14 days after inoculating, you can see it's done well where the LC ended up (even the ring from the pool of it on the bottom of the jar) but it isn't really going anywhere from there.


For reference, the LC that was used for this was also used on an agar plate. Then a sample was taken from that agar plate onto a new agar plate, resulting in this:

I've also tried to do A2G with several different strains. Here's a picture of all the agar plates before the transfer. Each transfer used the entire plate with a tic-tac-to pattern to cut up the agar.

It has been 10 days, and these are all similarly just showing a bit of growth around the agar but that's it. Here's an example:

Each jar was PC'ed following the tek and has a single hole in it about the size of a dime with micropore tape on the front and back of the hole:

The jars are all kept in my computer closet which is typically around 72 degrees. They are inside a drawer that is left cracked open for air but no light.
Here are the grains I have been using, I don't see any mention on the bag of antifungal coating or anything added to them:


Any feedback or advice is greatly welcome, what's going wrong?!?
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Kinoko314
Stranger Danger



Registered: 12/16/22
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Re: BOD's Easy AF OAT prep tek. [Re: Shroosier]
#28465421 - 09/11/23 01:29 PM (4 months, 14 days ago) |
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It looks like that first jar just needs a good shake. Still, it seems a bit slow.
Did you try washing the grain this time? I still think it would be worth a try. Even if it doesn't say anything on the package.
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Shroosier
Stranger

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Re: BOD's Easy AF OAT prep tek. [Re: Kinoko314]
#28465430 - 09/11/23 01:40 PM (4 months, 14 days ago) |
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Yes, for this batch I rinsed the oats several times before boiling. I went ahead and gave it a shake after taking the picture, I had two other jars I had inoculated at the same time (different strains) that also seemed to be stalling out.
Just for comparison, I split all the LC I had between two jars for each strain and kept one jar of each type in a bin on my back porch, they all showed similar progress to the ones I’ve been keeping in my normal spot.
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CrayolaHalls



Registered: 03/23/10
Posts: 216
Loc: The Lot
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Re: BOD's Easy AF OAT prep tek. [Re: Shroosier]
#28465899 - 09/11/23 09:51 PM (4 months, 14 days ago) |
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Shroosier...
The oats you are using should not have any detrimental levels of pesticide or fungicide.
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