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Dyn-A-Mite said:
you could but you don't want to get the filter wet. how are you going to make the LC?

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The filter should/may have contams on it if it is doing it's job. poly fill filters don't stop liquid from escaping the jar when shaken. It would have to be ungodly tight, and then you would be restricting GE. So if you shake the hell out of the jar chances are you are going to contam your LC.

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Josex said:
You don't need to shake, only swirl gently to break the mycelium with anything proper, get creative. You can use marbles, glass shards or whatever works. I've been using blender balls, they're awesome for the job.

Of course, a magnetic stirrer at max power will break the mycelium very easily in just a couple of minutes using a magnetic stir bar.

Polyfill sounds like the worst option possible and I think 4 or 5 layers of micropore tape would be much better, although it would still be far from ideal.

Whatman > SFD > MP tape > tyvek > polyfill

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Dyn-A-Mite said:
you could but you don't want to get the filter wet. how are you going to make the LC?

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Mad Season said:
Pfft if ya got plastic lids you probably don't even need a filter

Also I highly doubt a stuffed tight AF poly filter would have no gas exchange. LC and agar colonization is so small, it shouldn't even need gas exchange. I've grown many a dishes with literally no gas exchange holes, until you get into larger substrates, you won't see the myc stall out with such a small amount of media

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bodhisatta said:
maybe you don't really need to do LC

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