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PaulWall
Mush Love



Registered: 01/06/17
Posts: 98
Last seen: 7 years, 2 months
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Coconut water agar
#24111384 - 02/22/17 04:22 PM (7 years, 10 months ago) |
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I have searched and cant find much info on coconut water agar. Does anyone use coconut water? Is it possible? Seems very nutritious.
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JvF
Fletcher Detcher


Registered: 02/13/14
Posts: 2,662
Loc: Chicago
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Re: Coconut water agar [Re: PaulWall]
#24111400 - 02/22/17 04:30 PM (7 years, 10 months ago) |
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Hmm.
I have no experience whatsoever with this, but i dont see why it wouldnt work.
Though if you wanted to use a sugar based agar it wouls probably be a major disadvantage to use coconut water bwcausw of the cost.
Also if its overly nutritious it would be a bacterial breeding ground if your sterile technique wasnt perfect.
Eager to see what other peope contribute
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Panarchist
Cyanescendant



Registered: 04/02/16
Posts: 432
Loc: Copeland
Last seen: 1 year, 7 months
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Re: Coconut water agar [Re: JvF]
#24111998 - 02/22/17 08:38 PM (7 years, 10 months ago) |
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i don't think coconut water would add anything that you can't get from more affordable sources but it never hurts to experiment. you would have to try it in conjunction with some other more traditional agar recipes to get any kind of definable results though.
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PaulWall
Mush Love



Registered: 01/06/17
Posts: 98
Last seen: 7 years, 2 months
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Sure, just curious. I dont think it would be better than say a PDYA but i think it would be a fun experiment. Ill keep you guys updated
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Thedillestpickle
cultured



Registered: 02/02/16
Posts: 1,170
Loc: Canada
Last seen: 4 years, 6 months
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Re: Coconut water agar [Re: PaulWall]
#24112029 - 02/22/17 08:55 PM (7 years, 10 months ago) |
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I've been thinking lately that it would be handy to have ready to go beverages that could be used for making agar or LC This might be a good one to try. Maybe white grape juice would also work?
You definitely want to dilute the coconut water, keep that in mind. Check the nutritional information info on the label and do the math so that you have 3 to 4 grams of sugar per 100ml liquid after adding water.
Another idea I had was using dealcoholized beer to make LC or agar. The sugar to liquid ratio is pretty much spot on in most dealcoholized beer so in theory it would be ready to go right from the can. I've got two cans ready to try this next time I cook up agar.
Having these easy go to recipes just makes it a lot easier to create a variety of agar media, so that you can alternate food sources for the mycelium.
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vatman
I'm Vatman


Registered: 04/17/14
Posts: 1,642
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PaulWall
Mush Love



Registered: 01/06/17
Posts: 98
Last seen: 7 years, 2 months
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Quote:
Thedillestpickle said: I've been thinking lately that it would be handy to have ready to go beverages that could be used for making agar or LC This might be a good one to try. Maybe white grape juice would also work?
You definitely want to dilute the coconut water, keep that in mind. Check the nutritional information info on the label and do the math so that you have 3 to 4 grams of sugar per 100ml liquid after adding water.
Another idea I had was using dealcoholized beer to make LC or agar. The sugar to liquid ratio is pretty much spot on in most dealcoholized beer so in theory it would be ready to go right from the can. I've got two cans ready to try this next time I cook up agar.
Having these easy go to recipes just makes it a lot easier to create a variety of agar media, so that you can alternate food sources for the mycelium.
I agree 100% Its great to have a nice variety for the myc
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PaulWall
Mush Love



Registered: 01/06/17
Posts: 98
Last seen: 7 years, 2 months
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Re: Coconut water agar [Re: vatman]
#24112057 - 02/22/17 09:05 PM (7 years, 10 months ago) |
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Quote:
vatman said: Agar
Wow that is an incredible list. Thank you
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Thedillestpickle
cultured



Registered: 02/02/16
Posts: 1,170
Loc: Canada
Last seen: 4 years, 6 months
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Re: Coconut water agar [Re: vatman]
#24112062 - 02/22/17 09:08 PM (7 years, 10 months ago) |
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Quote:
vatman said: Agar
The recipe on that page is terrible for growing mycelium. The sugar content is 0.05% which is extremely low compared to the usual 4% and the agar to water ratio is about 1/10th what we typically use for pouring plates... so it's going be a liquidy runny mess Coconut Agar
CN
H2O
500 mL
Agar
1 g
Coconut milk/juice
10 g
If we are talking unsweetened coconut water, then I would actually use undiluted, I said to dilute it earlier, but then I just looked up the nutrient content and it's actually quite low in sugar. But it does have 0.7grams of protein so it's going to work quite well I'm sure. https://ndb.nal.usda.gov/ndb/foods/show/3665?fgcd=&manu=&lfacet=&format=&count=&max=50&offset=&sort=default&order=asc&qlookup=12119&ds=&qt=&qp=&qa=&qn=&q=&ing=
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PaulWall
Mush Love



Registered: 01/06/17
Posts: 98
Last seen: 7 years, 2 months
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Yea it seemed like only 1g of agar would not be enough at all
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vatman
I'm Vatman


Registered: 04/17/14
Posts: 1,642
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Quote:
Thedillestpickle said:
Quote:
vatman said: Agar
The recipe on that page is terrible for growing mycelium. The sugar content is 0.05% which is extremely low compared to the usual 4% and the agar to water ratio is about 1/10th what we typically use for pouring plates... so it's going be a liquidy runny mess Coconut Agar
CN
H2O
500 mL
Agar
1 g
Coconut milk/juice
10 g
If we are talking unsweetened coconut water, then I would actually use undiluted, I said to dilute it earlier, but then I just looked up the nutrient content and it's actually quite low in sugar. But it does have 0.7grams of protein so it's going to work quite well I'm sure. https://ndb.nal.usda.gov/ndb/foods/show/3665?fgcd=&manu=&lfacet=&format=&count=&max=50&offset=&sort=default&order=asc&qlookup=12119&ds=&qt=&qp=&qa=&qn=&q=&ing=
"MEDIA NOT TO USE!" Coconutwater I missed that myself. Seems like aloha doesn't use it for anything.
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Glomus
Stranger

Registered: 06/24/23
Posts: 81
Last seen: 1 year, 6 months
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Re: Coconut water agar [Re: PaulWall]
#28380609 - 06/30/23 07:55 PM (1 year, 6 months ago) |
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Coconut oils have anti fungal and bacterial properties to them. So the milk definitely wouldn't work. As for the water I'd imagine its a similar case.
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smalltalk_canceled
Babnik



Registered: 07/13/20
Posts: 3,174
Last seen: 18 hours, 5 minutes
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Re: Coconut water agar [Re: PaulWall]
#28380696 - 06/30/23 08:48 PM (1 year, 6 months ago) |
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Whats the purpose of this
-------------------- Willpower is the one true virtue
  
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