Home | Community | Message Board

Original Seeds Store
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: OlympusMyco.com Olympus Myco Bulk Substrate   Bridgetown Botanicals Bridgetown Botanicals   Original Sensible Seeds Autoflowering Cannabis Seeds   Kraken Kratom Red Vein Kratom   North Spore Bulk Substrate   PhytoExtractum Buy Bali Kratom Powder   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Mono Tub Substrate   Myyco.com Golden Teacher Liquid Culture For Sale   MagicBag.co All-In-One Bags That Don't Suck

Jump to first unread post Pages: 1 | 2 | 3  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlinemiksitk
Undercover Dopamine Molecule
I'm a teapot


Registered: 02/20/17
Posts: 24
Loc: Space
Last seen: 7 years, 27 days
Dmt to raise psilocybin content?
    #24109260 - 02/21/17 07:22 PM (7 years, 2 months ago)

Hey i herd rumors about dmt in the substrate raising active compound content by as much as 300% (1% to 3%). Is anybody experienced with this, and how practical is it to use canary reed grass as straw in my substrate to accomplish this for my next grow? Anybody who's gone down this path pls contribute to the community knowledge.


--------------------
:sun::confused::rasta::tongue::syringe::mad::mushroom2::mushroom2::shocked::thumbup:

Extras: Filter Print Post Top
Invisibleamidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
Re: Dmt to raise psilocybin content? [Re: miksitk] * 1
    #24109278 - 02/21/17 07:33 PM (7 years, 2 months ago)

.

Edited by amidogen (06/21/18 11:02 AM)

Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
Trusted Cultivator
Re: Dmt to raise psilocybin content? [Re: miksitk] * 1
    #24113231 - 02/23/17 10:53 AM (7 years, 2 months ago)

You should be forced to use the search engine 3 times before posting.


--------------------
Welcome to the Advanced Mycology Forum

Bod's Library    Article behind a paywall? I can try to get it for you

Extras: Filter Print Post Top
Offlinemiksitk
Undercover Dopamine Molecule
I'm a teapot


Registered: 02/20/17
Posts: 24
Loc: Space
Last seen: 7 years, 27 days
Re: Dmt to raise psilocybin content? [Re: bodhisatta]
    #24115173 - 02/24/17 02:34 AM (7 years, 2 months ago)

bodhi I've seen you around and your a dick bro, all just hateful and noncontrabutive posts that I've seen


--------------------
:sun::confused::rasta::tongue::syringe::mad::mushroom2::mushroom2::shocked::thumbup:

Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
Trusted Cultivator
Re: Dmt to raise psilocybin content? [Re: miksitk] * 3
    #24115639 - 02/24/17 09:21 AM (7 years, 2 months ago)

I only come off as a dick to people who seem underage.
I've seen you post dumb shit around in the wrong spots :shrug:


--------------------
Welcome to the Advanced Mycology Forum

Bod's Library    Article behind a paywall? I can try to get it for you

Extras: Filter Print Post Top
Offlinechaka333
mountain grunt.
Male User Gallery

Registered: 07/16/12
Posts: 1,097
Loc: on a hill in a pasture.
Last seen: 8 months, 6 days
Re: Dmt to raise psilocybin content? [Re: miksitk]
    #24115666 - 02/24/17 09:33 AM (7 years, 2 months ago)

Quote:

miksitk said:
bodhi I've seen you around and your a dick bro, all just hateful and noncontrabutive posts that I've seen




Says the guy with 12 posts, and has only been here for four days. Bodhisatta is the man. Don't waste your time with canary reed grass. Your mushrooms dont need to be more potent. Just eat more of them.


--------------------
If you want to achieve greatness
Stop asking for permission.

Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
Trusted Cultivator
Re: Dmt to raise psilocybin content? [Re: chaka333]
    #24115668 - 02/24/17 09:34 AM (7 years, 2 months ago)

So non contributive that the shroomery has me editing their dated info on the main page lol.


--------------------
Welcome to the Advanced Mycology Forum

Bod's Library    Article behind a paywall? I can try to get it for you

Extras: Filter Print Post Top
Invisibleamidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
Re: Dmt to raise psilocybin content? [Re: bodhisatta]
    #24115692 - 02/24/17 09:40 AM (7 years, 2 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.

Edited by amidogen (06/21/18 10:58 AM)

Extras: Filter Print Post Top
InvisibleGerms
Space Force
 User Gallery

Registered: 06/26/11
Posts: 4,607
Loc: Texas
Trusted Cultivator
Re: Dmt to raise psilocybin content? [Re: amidogen]
    #24116503 - 02/24/17 03:43 PM (7 years, 2 months ago)

This same guy private messaged me "your retarded af" after we all shut him down on a different thread where he asked what he could use or steal for an emergency substrate.

Typical underage noob to try and change the tek because of inability to follow the instructions and then whine when other members chime in.


--------------------

Extras: Filter Print Post Top
Offlineymca
Stranger


Registered: 03/29/16
Posts: 131
Last seen: 2 years, 10 months
Re: Dmt to raise psilocybin content? [Re: Germs]
    #24117166 - 02/24/17 08:00 PM (7 years, 2 months ago)

You're a dick bodh :rofl:


--------------------
Mystery strain's grow log

Extras: Filter Print Post Top
Offlinelovelaughlibs
Dopamine Slave
I'm a teapot User Gallery


Registered: 10/14/15
Posts: 1,811
Loc: England Flag
Last seen: 6 years, 3 months
Re: Dmt to raise psilocybin content? [Re: ymca]
    #24117375 - 02/24/17 09:22 PM (7 years, 2 months ago)

Sadly it doesn't work like that. Gotta rely on the mushroom's genetics to biosynthesise the active alkalkoids :smile:


--------------------
Ask and ye shall receive; Seek and ye shall find.

Extras: Filter Print Post Top
OfflineLennybernadino
Amazon grower
Male User Gallery

Registered: 09/16/09
Posts: 772
Loc: Iquitos, Peru
Last seen: 27 days, 4 hours
Re: Dmt to raise psilocybin content? [Re: lovelaughlibs]
    #24118122 - 02/25/17 07:22 AM (7 years, 2 months ago)

What I would try is this, Find a way to use or incorporate Phragmites communis rhizomes as a/into your substrate. Grow side by side with other substrate that does not contain triptymine rich ingredients(choir) and see if there is a difference. It would be stupid to extract pure dmt for this purpose, but using unextracted tryptamine rich substrate would be a better idea.

Edited by Lennybernadino (02/25/17 07:22 AM)

Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
Trusted Cultivator
Re: Dmt to raise psilocybin content? [Re: Lennybernadino]
    #24118262 - 02/25/17 08:59 AM (7 years, 2 months ago)

Same reasons shrooms don't bioaccumulate toxins from their environment or substrate. They're not assimilating big molecules like tryptamines.


--------------------
Welcome to the Advanced Mycology Forum

Bod's Library    Article behind a paywall? I can try to get it for you

Extras: Filter Print Post Top
OfflineLennybernadino
Amazon grower
Male User Gallery

Registered: 09/16/09
Posts: 772
Loc: Iquitos, Peru
Last seen: 27 days, 4 hours
Re: Dmt to raise psilocybin content? [Re: bodhisatta] * 1
    #24120235 - 02/26/17 01:43 AM (7 years, 2 months ago)

Scientific assumption vs. technique. Assumption assumes that this has been figured out, technique just does the experiment anyway and observes results.

Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
Trusted Cultivator
Re: Dmt to raise psilocybin content? [Re: Lennybernadino]
    #24120860 - 02/26/17 10:00 AM (7 years, 2 months ago)

It's not assumption it's shit they figured out even earlier than the 60s. How fungi digests things extracellularly then absorbs.

Then it was empericaly tested 100s of times by real mycologists and really killed that idea.

Then it was tested 1000s more times since it crosses every noobs mind but only some noobs are smart enough to do a 4th grader's level of research on a search engine, thus avoiding repeating history


--------------------
Welcome to the Advanced Mycology Forum

Bod's Library    Article behind a paywall? I can try to get it for you

Edited by bodhisatta (02/26/17 10:10 AM)

Extras: Filter Print Post Top
OfflineBasilBush
Reality Bender
Male User Gallery


Registered: 11/13/13
Posts: 928
Last seen: 1 month, 28 days
Re: Dmt to raise psilocybin content? [Re: bodhisatta]
    #24123249 - 02/27/17 10:35 AM (7 years, 2 months ago)

Thanks all, was a good read lol made me smile as I imagined shroomers arguing.


--------------------
I HUNT MAGIC MUSHROOMS AND MAKE MIND BLOWING TEA,
I BUY ROOT BARK OFF THE INTERNET AND EXTRACT DMT,
SMOKE MY FRIENDS CHANGA IT TASTES BETTER FOR FREE
TRIPPING IS WONDERFUL IT HELPS ME FIND PEACE,
SO WILL YOU COME ALONG TO MY NEXT EUPHORIC FEAST.

I LOVE MUSHROOMS THEY MUSH UP THE ROOM THERE AINT MUCH ROOM IN HERE
SPECIAL GREEN TEA MADE FROM A CACTUS TREE SETS YOUR MIND AND SPIRIT FREE

Extras: Filter Print Post Top
OfflinePinPornProducer
Buy the ticket, take the ride
Male User Gallery


Registered: 08/23/14
Posts: 9,981
Loc: Rocky Point R.I Flag
Last seen: 6 years, 4 months
Re: Dmt to raise psilocybin content? [Re: miksitk]
    #24123304 - 02/27/17 10:50 AM (7 years, 2 months ago)

Quote:

miksitk said:
bodhi I've seen you around and your a dick bro, all just hateful and noncontrabutive posts that I've seen



:nutkick:

Extras: Filter Print Post Top
Offlinepursuitoperception
Humble Student
Male User Gallery


Registered: 01/10/17
Posts: 14
Loc: PNW, OREGON
Last seen: 4 years, 7 days
Re: Dmt to raise psilocybin content? [Re: PinPornProducer]
    #24136913 - 03/04/17 06:01 PM (7 years, 2 months ago)

This should be stickied in the "how not to conduct yourself on forums" section...:eek:

Extras: Filter Print Post Top
OfflineMrcloudy
Stranger than you.
Male User Gallery

Registered: 10/01/13
Posts: 2,889
Loc: Northeast US Flag
Last seen: 8 days, 13 hours
Re: Dmt to raise psilocybin content? [Re: miksitk] * 1
    #24137276 - 03/04/17 08:54 PM (7 years, 2 months ago)

Quote:

miksitk said:
bodhi I've seen you around and your a dick bro, all just hateful and noncontrabutive posts that I've seen



:awewtf:


--------------------

10 different Ganoderma species from across the USA

AMU

MrCloudys guide to North American GanodermaUpdated A rough guide to North American Ganoderma species, with an emphasis on the laccate species.

Extras: Filter Print Post Top
Offlinepeyotillo
Autodidact

Registered: 07/19/14
Posts: 176
Loc: Yurp
Last seen: 1 year, 10 months
Re: Dmt to raise psilocybin content? [Re: Mrcloudy]
    #24137818 - 03/05/17 05:38 AM (7 years, 2 months ago)

Who would want to turn DMT into psilocybin/psilocin anyway?

If anything, it should be the other way around!

PS: if you know this magic technique i would love to talk to you about it but from my average organic chemistry this is not possible (beyond the means of the kitchen chemists). I remember all the amusing threads on "how to convert x", some even said they turned psilocybin into 4aco just by adding vinegar. That's about the majorities level of education on these forums

Cheers


--------------------
Far above the Moon, planet Earth is blue and there's nothing i can do

Extras: Filter Print Post Top
Invisiblewildernessjunkie
Reshitivest
I'm a teapot

Registered: 06/13/10
Posts: 8,118
Loc: HTTP 404 Not Found
Trusted Cultivator
Re: Dmt to raise psilocybin content? [Re: peyotillo]
    #24165877 - 03/16/17 01:26 AM (7 years, 2 months ago)

Quote:

Hey i herd rumors about dmt in the substrate raising active compound content by as much as 300% (1% to 3%)




Rumors are an amazing thing. Rarely grounded in any actual real science. Can you cite the source of this "rumor"?

Extras: Filter Print Post Top
OfflineKThunderland
Dude
Male User Gallery

Registered: 12/21/11
Posts: 451
Loc: CT
Last seen: 1 year, 6 months
Re: Dmt to raise psilocybin content? [Re: wildernessjunkie]
    #24165992 - 03/16/17 03:33 AM (7 years, 2 months ago)

Over the last few yearsthis has seemed to come up about a few dozen times,  maybe monthly, its just doesnt work dmt wont raise the potency at all (unless u sprinkle it on the shrooms after and ingest with aan maoi lol)


--------------------
Check bio for trade list. Always open to trade anything of mine (that i can )for BTC.

Extras: Filter Print Post Top
OfflineBlueDisa
Friend
 User Gallery

Registered: 03/02/17
Posts: 170
Last seen: 14 days, 12 hours
Re: Dmt to raise psilocybin content? [Re: KThunderland]
    #24166131 - 03/16/17 05:34 AM (7 years, 2 months ago)

.
Quote:

KThunderland said:
Over the last few yearsthis has seemed to come up about a few dozen times,  maybe monthly, its just doesnt work dmt wont raise the potency at all (unless u sprinkle it on the shrooms after and ingest with aan maoi lol)




or just consume shrooms with MAOI, that combo blasts one a good distance into space

Extras: Filter Print Post Top
Invisiblehowsyournaggerdoin
Happy


Registered: 02/04/16
Posts: 1,600
Re: Dmt to raise psilocybin content? [Re: BlueDisa]
    #24166169 - 03/16/17 06:24 AM (7 years, 2 months ago)

If you smoke the shrooms theyre almost like dmt i swear (LOL)

Extras: Filter Print Post Top
OfflineKThunderland
Dude
Male User Gallery

Registered: 12/21/11
Posts: 451
Loc: CT
Last seen: 1 year, 6 months
Re: Dmt to raise psilocybin content? [Re: howsyournaggerdoin]
    #24167398 - 03/16/17 03:24 PM (7 years, 2 months ago)

Ha lmao


--------------------
Check bio for trade list. Always open to trade anything of mine (that i can )for BTC.

Extras: Filter Print Post Top
Offlinekrypto2000
Unknown


Registered: 12/05/06
Posts: 11,579
Last seen: 4 years, 6 months
Re: Dmt to raise psilocybin content? [Re: KThunderland] * 1
    #24169475 - 03/17/17 10:20 AM (7 years, 2 months ago)

I have seen a report or two claiming this worked, the original and possibly one other. I have seen no one else try it so how can anyone here claim it's been tried to death and fails every time? Why dont you find a reference for these failurs if the info is so abundant?

What has been tried is tryptophan and even that not working doesn't mean it can't work or that the study saying it does is bunk. It just means it couldn't be reproduced with random genetics, it could well be reproduced with the same culture used for the original study.

What's been tried more but still not enough for me to conclusively say it doesn't work is tryptamine. The metobolic pathway, iirc, is tryptophan -> tryptamine -> n,n,dmt -> 4-ho-dmt -> 4-po-dmt. Some of those steps are metabolically limited, some may well not be. Starting further down the chain sure increases your chances of bioconversion. In addition to the original source shulgin also seemed to believe this is viable so until I see a well controlled test showing failure I'm not going to assume it would.

Extras: Filter Print Post Top
OfflineBasilBush
Reality Bender
Male User Gallery


Registered: 11/13/13
Posts: 928
Last seen: 1 month, 28 days
Re: Dmt to raise psilocybin content? [Re: krypto2000]
    #24169723 - 03/17/17 11:45 AM (7 years, 2 months ago)

I saved up all the yellow myc piss from 3-4 grows in the freezer. It formed glass shard like crystals.  I tell you know tho shit is as strong or on the same level as dmt, blew my mind. I now stress out all my cakes and smoke the myc piss, why don't you all try it.

Extras: Filter Print Post Top
OfflineKThunderland
Dude
Male User Gallery

Registered: 12/21/11
Posts: 451
Loc: CT
Last seen: 1 year, 6 months
Re: Dmt to raise psilocybin content? [Re: BasilBush]
    #24169895 - 03/17/17 12:54 PM (7 years, 2 months ago)

Ok tons of ppl have tried it myslef included sorry to say any increase was not noticeable, not even a minor increase that id expect from placebo effect, ppl have even used dmt grasses in place of straw,  etc, but tge science isnt there it, with tge way mushroom digestion works and tge way the alkoids are produced the science just inst there. The best way to increase potency is to provide the optimum conditions and select for best potency. Or grow pe.


--------------------
Check bio for trade list. Always open to trade anything of mine (that i can )for BTC.

Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
Trusted Cultivator
Re: Dmt to raise psilocybin content? [Re: KThunderland]
    #24169906 - 03/17/17 12:57 PM (7 years, 2 months ago)

And understanding of biochemistry of fungi kind of negates it too


--------------------
Welcome to the Advanced Mycology Forum

Bod's Library    Article behind a paywall? I can try to get it for you

Extras: Filter Print Post Top
Offlinekrypto2000
Unknown


Registered: 12/05/06
Posts: 11,579
Last seen: 4 years, 6 months
Re: Dmt to raise psilocybin content? [Re: KThunderland]
    #24170005 - 03/17/17 01:31 PM (7 years, 2 months ago)

Quote:

KThunderland said:
Ok tons of ppl have tried it myslef included sorry to say any increase was not noticeable, not even a minor increase that id expect from placebo effect, ppl have even used dmt grasses in place of straw,  etc, but tge science isnt there it, with tge way mushroom digestion works and tge way the alkoids are produced the science just inst there. The best way to increase potency is to provide the optimum conditions and select for best potency. Or grow pe.




Can you describe exactly what you did? Someone saying, "I tried it and it didn't work" isn't reason enough to assume it doesn't work, if you share you exact procedure and it seems sound then we can cite that as one instance that it probably failed, but without verifying you even did it correctly it doesn't mean much of anything.

Using wild dmt containing grasses, which tend to have a very very low dmt content, means nothing. Using a kilo of dried grass could introduce as little as a hundred milligrams of dmt to the substrate, if your whole first flush produced 5 grams of psilocybin then an extra 500mg is going to be negligible even if it had a 100% uptake and conversion rate.

Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
Trusted Cultivator
Re: Dmt to raise psilocybin content? [Re: krypto2000]
    #24170073 - 03/17/17 02:09 PM (7 years, 2 months ago)

It's amazing how far gartz pf and staments have set back the hobby but you can't talk bad about them because they did have great contributions.


--------------------
Welcome to the Advanced Mycology Forum

Bod's Library    Article behind a paywall? I can try to get it for you

Extras: Filter Print Post Top
Invisibleamidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
Re: Dmt to raise psilocybin content? [Re: BasilBush]
    #24170286 - 03/17/17 03:41 PM (7 years, 2 months ago)

Quote:

BasilBush said:
I saved up all the yellow myc piss from 3-4 grows in the freezer. It formed glass shard like crystals.  I tell you know tho shit is as strong or on the same level as dmt, blew my mind. I now stress out all my cakes and smoke the myc piss, why don't you all try it.



:awewtf:


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.

Extras: Filter Print Post Top
OfflineBasilBush
Reality Bender
Male User Gallery


Registered: 11/13/13
Posts: 928
Last seen: 1 month, 28 days
Re: Dmt to raise psilocybin content? [Re: amidogen]
    #24170336 - 03/17/17 04:00 PM (7 years, 2 months ago)

He he ha ha ha


Myc piss I the way to go, dont knock it till youve tried it

Extras: Filter Print Post Top
Invisibleamidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
Re: Dmt to raise psilocybin content? [Re: BasilBush]
    #24170343 - 03/17/17 04:03 PM (7 years, 2 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.

Edited by amidogen (06/21/18 10:26 AM)

Extras: Filter Print Post Top
OfflineKThunderland
Dude
Male User Gallery

Registered: 12/21/11
Posts: 451
Loc: CT
Last seen: 1 year, 6 months
Re: Dmt to raise psilocybin content? [Re: amidogen]
    #24170346 - 03/17/17 04:03 PM (7 years, 2 months ago)

There isnt two reports, hell right now there is 6 on my screen below. Not to be rude but the reason ppl arent having this dicussion again because e we are tired of it. Its doesnt work, dmt is not utilized by tge mycellium to produce alkaloids.  Thats all.


--------------------
Check bio for trade list. Always open to trade anything of mine (that i can )for BTC.

Extras: Filter Print Post Top
Invisibleamidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
Re: Dmt to raise psilocybin content? [Re: KThunderland]
    #24170352 - 03/17/17 04:06 PM (7 years, 2 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.

Edited by amidogen (06/21/18 10:26 AM)

Extras: Filter Print Post Top
Offlinekrypto2000
Unknown


Registered: 12/05/06
Posts: 11,579
Last seen: 4 years, 6 months
Re: Dmt to raise psilocybin content? [Re: KThunderland]
    #24170527 - 03/17/17 05:09 PM (7 years, 2 months ago)

Quote:

KThunderland said:
There isnt two reports, hell right now there is 6 on my screen below. Not to be rude but the reason ppl arent having this dicussion again because e we are tired of it. Its doesnt work, dmt is not utilized by tge mycellium to produce alkaloids.  Thats all.




I see 5 and not one has any experiments, data, or even novel discussion on the topic beyond where this thread has gone. That's what you consider to be valid scientific reports? I'd still like to hear a summary of your experiment.

Extras: Filter Print Post Top
Invisiblewildernessjunkie
Reshitivest
I'm a teapot

Registered: 06/13/10
Posts: 8,118
Loc: HTTP 404 Not Found
Trusted Cultivator
Re: Dmt to raise psilocybin content? [Re: krypto2000]
    #24170587 - 03/17/17 05:43 PM (7 years, 2 months ago)

The idea with the metabolites is interesting. I might give that a shot at some point, just for kicks.

I'd also like to point out that no one needs to explain why a concept doesn't work. If it doesn't work, then it doesn't work. The burden of proof falls on the person that insists that it does work, or that it should work.

There's still a lot of things we don't understand about mushrooms. And until someone that has the skills and access to items such as Chromatography equipment, we can largely only go by subjective review.

Extras: Filter Print Post Top
OfflineKThunderland
Dude
Male User Gallery

Registered: 12/21/11
Posts: 451
Loc: CT
Last seen: 1 year, 6 months
Re: Dmt to raise psilocybin content? [Re: wildernessjunkie]
    #24170756 - 03/17/17 06:53 PM (7 years, 2 months ago)

NNDMT, the DMT found in most ayahuasca plants, particularly mimosa hostilis, is in fact an inhibitor of the enyzyme tryptophan decarboxylase which creates the tryptamines necessary in the production of psilocin + psilocybin. And  Dude no one is attacking you, we all thought about this at some point lol


--------------------
Check bio for trade list. Always open to trade anything of mine (that i can )for BTC.

Extras: Filter Print Post Top
Offlinekrypto2000
Unknown


Registered: 12/05/06
Posts: 11,579
Last seen: 4 years, 6 months
Re: Dmt to raise psilocybin content? [Re: wildernessjunkie]
    #24170785 - 03/17/17 07:07 PM (7 years, 2 months ago)

If that idea came about from a tickle in someone's asshole and nothing else then you'd totally be right, the burden of proof is on them. That's not what happened though, we're debating whether an already existing piece of evidence conducted by a professional biochemist is valid or not. They ran an experiment and got a result, their experiment may be flawed, the result may be wrong, but somehow they got said result so if someone wants to prove that result wrong then the burden of proof rests on them, not the other way around. The person trying to prove it has already done so.

I'm not trying to prove it one way or the other, I have no opinion on it. I'm just trying to get people to accept that we don't know, that they can't know, and to not make the unsupported claim that it doesn't work when their only reason for believing so is because they haven't seen anyone reproduce it while they're unable to cite anyone even trying to reproduce it.

If you re-run their experiment and cannot reproduce the results then we have reason to doubt it, but still nothing could be concluded from a single attempt and in cases like that it's much more likely the experiment showing a result is correct and the attempt at reproduction is flawed because it's far more likely to miss something that is there than it is to find something that isn't. Just think about that. A lot of variables need to be just right for an experiment to be successful, but only one variable must be wrong in order for it to fail and a bunch of wookies on a drug forum are not the most scientifically rigorous group of people.

If one ran multiple independent tests and still are unable to reproduce it, one comes to understand biologically why it will or will not work, or determines a confounding flaw in the original experiment then we can reasonably conclude it doesn't work. Until then we can't and at best (or worst) we just have to say it may or may not, but it's simply an unknown. A bunch of threads with people circle jerking over their interpretation of the same study without even any new insights, much less actual tests, is not what I consider relevant data to add to the debate, I sure don't consider them scientifically valid reports.

The closest thing I've seen to someone trying to reproduce it is people dumping l-tryptophan into a multi-spore monotub, eating the fruits, and then based on their subjective experience claiming they're no different then their MS tub they grew a couple of months ago. They may not be different, but eating some random fruits is not a valid way to test for that, and that's also not even what the original experiment did as I remember it and so it wouldn't even be a valid reproduction.

For the record I have no opinion on whether it works or not, I do not and can not know, just like anyone else here despite what some may believe. If someone here has some information that would change that then please share it, but until then no one can claim one way or the other. Thunderland claims to have tried to reproduce it and failed, if he/she would share their methodology then I might agree that he has a valid reason to believe it was a flawed study, but if he can't even do that why would I assume he accurately reproduced it much less believe his results at face value? If he won't share then he still may have a valid reason to believe it's flawed, but why then should that change any of our opinions?

Extras: Filter Print Post Top
Offlinekrypto2000
Unknown


Registered: 12/05/06
Posts: 11,579
Last seen: 4 years, 6 months
Re: Dmt to raise psilocybin content? [Re: KThunderland]
    #24170798 - 03/17/17 07:13 PM (7 years, 2 months ago)

Quote:

KThunderland said:
NNDMT, the DMT found in most ayahuasca plants, particularly mimosa hostilis, is in fact an inhibitor of the enyzyme tryptophan decarboxylase which creates the tryptamines necessary in the production of psilocin + psilocybin. And  Dude no one is attacking you, we all thought about this at some point lol




What does that have to do with mushrooms phosphorylating added tryptamines though? If you're converting n,n,dmt into 4-ho-dmt (or 4-po-dmt) then it makes no difference if the metabolic pathway to create typtophan, tryptamine, or any of the other precursors is inhibited or shut down completely, that part of the biosynthesis has already been bypassed completely anyway. All we need to happen is the last step of hydroxylation and/or phosphorylation.

I don't think anyone is attacked me, nor am I attacking anyone else, and I do apologize if I've come across that way, I'm just frustrated by some of the illogical conclusions some are claiming. If it's a logical conclusion to you then please share the information that created that logic so the rest of us can arrive there.

edit: And why would the dmt in mimosa hostilis be anymore inhibiting than any other dmt anyway? N,N,DMT is N,N,DMT no matter the source.

Extras: Filter Print Post Top
Invisiblewildernessjunkie
Reshitivest
I'm a teapot

Registered: 06/13/10
Posts: 8,118
Loc: HTTP 404 Not Found
Trusted Cultivator
Re: Dmt to raise psilocybin content? [Re: krypto2000] * 2
    #24170830 - 03/17/17 07:26 PM (7 years, 2 months ago)

Disclaimer: I'm on my phone, and the green beer is kicking in.

I wasn't attacking you, please don't think that. But any number of claims can be made in regards to mush cult.

Here's how I see it. If there was some secret trick to producing significantly more magic to any substrate, then it wouldn't be a secret for long. And right now, as it stands, the true and actual numbers of potency are more of a mystery than actual science.  (Though I think we have a good idea on yeild.)

Until we can attach actual scientifically derived numbers to any claim of potency...its all subjective.

I'm not saying its not possible. I'm just saying its not proven. Numbers are important.

Extras: Filter Print Post Top
OfflineKThunderland
Dude
Male User Gallery

Registered: 12/21/11
Posts: 451
Loc: CT
Last seen: 1 year, 6 months
Re: Dmt to raise psilocybin content? [Re: wildernessjunkie]
    #24170857 - 03/17/17 07:39 PM (7 years, 2 months ago)

Your implying that what i said implied that any nndmt would be any better, it wouldnt.


--------------------
Check bio for trade list. Always open to trade anything of mine (that i can )for BTC.

Extras: Filter Print Post Top
Offlinekrypto2000
Unknown


Registered: 12/05/06
Posts: 11,579
Last seen: 4 years, 6 months
Re: Dmt to raise psilocybin content? [Re: KThunderland]
    #24170892 - 03/17/17 07:56 PM (7 years, 2 months ago)

Quote:

wildernessjunkie said:
Disclaimer: I'm on my phone, and the green beer is kicking in.

I wasn't attacking you, please don't think that. But any number of claims can be made in regards to mush cult.

Here's how I see it. If there was some secret trick to producing significantly more magic to any substrate, then it wouldn't be a secret for long. And right now, as it stands, the true and actual numbers of potency are more of a mystery than actual science.  (Though I think we have a good idea on yeild.)

Until we can attach actual scientifically derived numbers to any claim of potency...its all subjective.

I'm not saying its not possible. I'm just saying its not proven. Numbers are important.




I agree completely, well said.
Quote:

KThunderland said:
Your implying that what i said implied that any nndmt would be any better, it wouldnt.




I'm not sure what you mean? How did I imply that?

All I thought you were implying was that because dmt inhibits decarboxylation that it can't get metabolized into 4-po, which is not the case as decardboxylation and hydroxylation/phosporylation are entirely different steps. If you were not saying that though then how is it relevant in regards to mushrooms metabolizing dmt to psilocybin?

Extras: Filter Print Post Top
OfflineKThunderland
Dude
Male User Gallery

Registered: 12/21/11
Posts: 451
Loc: CT
Last seen: 1 year, 6 months
Re: Dmt to raise psilocybin content? [Re: krypto2000]
    #24170922 - 03/17/17 08:07 PM (7 years, 2 months ago)

Nndmt inhibits the enzyme that the mushroom uses to create psilocin + psilocybin.sorry if i wasnt clear i had to reread it myself, worst case it hurts best case you have wasted dmt... Or tbh increased potency by some yet unknown mechanism.


--------------------
Check bio for trade list. Always open to trade anything of mine (that i can )for BTC.

Extras: Filter Print Post Top
OfflineBasilBush
Reality Bender
Male User Gallery


Registered: 11/13/13
Posts: 928
Last seen: 1 month, 28 days
Re: Dmt to raise psilocybin content? [Re: KThunderland]
    #24171428 - 03/18/17 01:36 AM (7 years, 2 months ago)

I read this type of post numerous time before and everyone always argues about it, or just like me they havnt got a clue.
That crystal in the petri dish is salt I coloured on with a felt tip pen just for giggles,I've come to the conclusion by pure guessing. Smoking myc piss would be more effective then none scientific noobs and amateurs (like me) putting chemicals in the substrate.

I would piss my self if someone actualy smoked myc piss and was still high from last night.

Sorry all for getting ya hopes up, just my shitty sense of humor finally coming thru.

Extras: Filter Print Post Top
Invisibleamidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
Re: Dmt to raise psilocybin content? [Re: BasilBush] * 1
    #24171442 - 03/18/17 01:52 AM (7 years, 2 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.

Edited by amidogen (06/21/18 10:25 AM)

Extras: Filter Print Post Top
Offlinekrypto2000
Unknown


Registered: 12/05/06
Posts: 11,579
Last seen: 4 years, 6 months
Re: Dmt to raise psilocybin content? [Re: KThunderland]
    #24172056 - 03/18/17 11:05 AM (7 years, 2 months ago)

Quote:

KThunderland said:
Nndmt inhibits the enzyme that the mushroom uses to create psilocin + psilocybin.sorry if i wasnt clear i had to reread it myself, worst case it hurts best case you have wasted dmt... Or tbh increased potency by some yet unknown mechanism.




It does not inhibit psilocin or psilocybin production at all though, that is not what your quote says. It inhibits tryptamine production. They are completely unrelated processes. If you are supplying dmt or some other tryptamine to the fungi then it does not matter if the fungi can produce it itself or not, it's already in the substrate because you put it there. There is no mention of dmt inhibiting phosphorylation or hydroxylation which is the only step remaining to convert dmt into a 4 subbed tryptamine, there is nothing to decarboxylate on dmt so it doesn't matter if the dexarcboxylase enzymes are absent.

That argument would be akin to not upgrading your 56k line to fiber internet bc in order to install the fiber they would have to rip out the old phone line. Yah you won't have dial up internet anymore but you'll still have internet, much faster internet.

Extras: Filter Print Post Top
OfflineKThunderland
Dude
Male User Gallery

Registered: 12/21/11
Posts: 451
Loc: CT
Last seen: 1 year, 6 months
Re: Dmt to raise psilocybin content? [Re: krypto2000]
    #24172130 - 03/18/17 11:37 AM (7 years, 2 months ago)

Dmt is a tryptamine, im sorry if you dont u dertsand the science behind ehat im sating but look it up before trying to talk it down, it looks foolish


--------------------
Check bio for trade list. Always open to trade anything of mine (that i can )for BTC.

Extras: Filter Print Post Top
Offlinekrypto2000
Unknown


Registered: 12/05/06
Posts: 11,579
Last seen: 4 years, 6 months
Re: Dmt to raise psilocybin content? [Re: KThunderland]
    #24172196 - 03/18/17 12:10 PM (7 years, 2 months ago)

Haha, you're clearly the one that doesn't understand here. I'm not going to keep repeating it. The very thread you got that information says the same thing that I have kept trying to explain to you if you'd have read it.

Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
Trusted Cultivator
Re: Dmt to raise psilocybin content? [Re: krypto2000]
    #24172212 - 03/18/17 12:17 PM (7 years, 2 months ago)

Re anecdotally said he tried it dozens of times. I trust him more than gartz and PF. So what they wrote a piss poor paper with flawed methodology that never once was replicated.

If it worked we would not be in the dark about it

Anyone asking about it should be producing new evidence or compelling research of their own. Otherwise it's just the same shit every few months


--------------------
Welcome to the Advanced Mycology Forum

Bod's Library    Article behind a paywall? I can try to get it for you

Extras: Filter Print Post Top
InvisibleKush_Zombie
smug piece of shit
 User Gallery


Registered: 10/22/14
Posts: 4,793
Re: Dmt to raise psilocybin content? [Re: bodhisatta]
    #24172215 - 03/18/17 12:20 PM (7 years, 2 months ago)

If you're seriously contemplating adding dmt to your substrate for stronger shrooms....

You should do it. :popcorn:


--------------------
How to get started in bulk:
Presto 23-Quart Pressure Cooker
BOD's Simple as FUCK Still Air Box
PastyWhyte's Easy Agar Tek
Munchauzen's Cultivation Video Series
How EvilMushroom666 Prepares His Grains (I use jars with Synthetic Filter Discs)
What is G2G? (Grain-to-Grain)
Damion5050's Coir Tek (I use 5.5 - 6 quarts of water instead of 4. Also ignore step 13 and ignore the monotub completely. The only purpose of this tek is to show you how to make a simple substrate. I also add gypsum to it but not necessary)
Spitball's Monotub Tek (A liner isn't necessary but is useful)
Use 6500k lights throughout the whole process. When you wake up, turn the light on. When you go to sleep turn the light off. It's as simple as that.

Extras: Filter Print Post Top
Offlinekrypto2000
Unknown


Registered: 12/05/06
Posts: 11,579
Last seen: 4 years, 6 months
Re: Dmt to raise psilocybin content? [Re: bodhisatta]
    #24172335 - 03/18/17 01:20 PM (7 years, 2 months ago)

Quote:

bodhisatta said:
Re anecdotally said he tried it dozens of times. I trust him more than gartz and PF. So what they wrote a piss poor paper with flawed methodology that never once was replicated.





You trust someone who hasn't been a member long, has no credibility, has demonstrated he doesn't even understand the metabolic pathway by repeating that decarboxylase inhibition rhetoric, and above all else will not even write a brief summary of their procedure despite numerous requests over two different people who have published their results? Having tried it multiple times doesn't mean anything, if he did it wrong the first time then repeating it will obviously not produce a new result.

Quote:



Anyone asking about it should be producing new evidence or compelling research of their own. Otherwise it's just the same shit every few months




I agree, but I didn't ask the question.

Extras: Filter Print Post Top
Invisiblec10h12n2o
serial dilutor
 User Gallery


Registered: 01/21/15
Posts: 3,200
Loc: the abyss
Re: Dmt to raise psilocybin content? [Re: wildernessjunkie]
    #24186317 - 03/23/17 02:25 PM (7 years, 1 month ago)

Quote:

wildernessjunkie said:
Until we can attach actual scientifically derived numbers to any claim of potency...its all subjective.

I'm not saying its not possible. I'm just saying its not proven. Numbers are important.




:whathesaid: 100%

its kinda silly that anyone would even presume to debate these things when it is so subjective (unless you have a chromatography lab and team handy)... and even then, even when dealing with identical doses of an identical substance, psychedelic trips are STILL subjective by their very nature, no one's experience, brain chemistry, or set & setting is identical

so "stronger" only has meaning when you ground it to something concrete, like active percentages. but even that is complicated, because (as we have been learning in the MMJ industry), the interaction between the various active compounds (as well as non-psychoactive), both agonists and antagonists, affecting our various brains, can have a tremendous impact on the overall perceived "strength" or "effects" (and psychadelics get even more complicated)

for instance, in cannabis there are cannabinoid agonists such as THC, antagonists such as Rimonabant, and at least 483 distinct compounds, only a few of which have been studied at all. not all plants have all the compounds, and their concentrations vary greatly. a plant might have 25% thc but feel less potent than a 13% thc plant because of some other active compounds, or perhaps it could be the interaction between agonists and antagonists (high thc and antagonist in the same sample)

Rimonabant is especially interesting because where THC makes you hungry, happy, sleepy, Rimonabant does the exact opposite, so it was marketed as a diet drug. but these not hungry, not happy, not sleepy people kept killing themselves, so it was pulled from the market. as we learn about these systems and substances that interact with them, it challenges a lot of basic assumptions we tend to make about "potency," especially when dealing with a natural, unrefined product

i think the biggest problem with Stamets & Gartz paper has been the way people misinterpret it and try to apply it as a technique to increase the potency of their crops, rather than seeing it for what it is: an interesting if potentially flawed study that brings up lots of questions, rather than a "potency tek"


--------------------

C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche

Extras: Filter Print Post Top
Offlinekrypto2000
Unknown


Registered: 12/05/06
Posts: 11,579
Last seen: 4 years, 6 months
Re: Dmt to raise psilocybin content? [Re: c10h12n2o]
    #24186406 - 03/23/17 03:02 PM (7 years, 1 month ago)

Yeah, I agree. As I recall in the original study they grew the mycilium in a liquid culture or a petri, fed the network with the alkaloids, and found it converted them to their 4-OH and 4-PO counterparts. They may not ever be incorprated into the fruit bodies, and there is some research saying that dmt specifically is not well incorporated even, so feeding them to a substrate would do nothing to make the fruits more potent, but that does not mean that the conversion is not taking place or the paper made false claims. To my knowledge it's never been faithfully reproduced to actually conclude one way or the other. Likewise as was mentioned dmt inhibits tryptamine production so if you added enough dmt to double the alkaloid content but the alkaloid content is halved due to the decreased production of decarboxylase enzymes then you would end up with the exact same potency as if you did nothing.

Extras: Filter Print Post Top
OfflineMarkostheGnostic
Elder
Male User Gallery


Registered: 12/09/99
Posts: 14,279
Loc: South Florida Flag
Last seen: 3 years, 3 months
Re: Dmt to raise psilocybin content? [Re: miksitk]
    #24188383 - 03/24/17 10:10 AM (7 years, 1 month ago)

When I was first attempting to grow P. cubensis in 1989, I asked a molecular biologist friend about how to increase potency, and he asked a colleague more familiar with the topic. My friend then sent me some some custom-made agar to which he added the amino acid L-Tryptophan. This was supposed to contribute to the production of psilocybin or psilocin (it's been a while I don't remember). I was new at mushroom growing at the time working exclusively out of Oss & Oeric's (Terrence and Dennis McKenna) little book Psilocybin: Magic Mushroom Growers Guide: A Handbook for Psilocybin Enthusiasts. This was before Paul Stamets published his books. I cannot say whether potency was increased, but the biochemistry of a natural precursor amino acid makes more sense to me than the adding a complete molecule of some foreign form of DMT which cannot be broken down and then reassembled by the mushroom. (I don't usually come to this forum, but I landed here, saw this, and put in my 2¢). :cheers: :bye:


--------------------
γνῶθι σαὐτόν - Gnothi Seauton - Know Thyself

Extras: Filter Print Post Top
Offlinekrypto2000
Unknown


Registered: 12/05/06
Posts: 11,579
Last seen: 4 years, 6 months
Re: Dmt to raise psilocybin content? [Re: MarkostheGnostic]
    #24188435 - 03/24/17 10:30 AM (7 years, 1 month ago)

It's not foreign though, it's a building block to psilocin and psilocybin but more complete than tryptophan. In cubensis it builds tryptophan, decarboxylates it into tryptamine which is then either phosphylated or hydroxylated and methylated into psilocybin/psilocin. Some other psychedelic mushrooms also produce dmt as an intermediate, but in cubensis I do believe the methylation either happens at the same time as the hydroxylation/phosphorylation or it happens afterward bc afaik there has not been any n,n dmt found in cubes. To put it another way the difference between psilocin and dmt is an oxygen atom added to psilocin, the difference between psilocybin is a phospor atom.

Extras: Filter Print Post Top
OfflineEywa_devotee
Goddess Worshiper
Male User Gallery

Registered: 10/04/10
Posts: 1,088
Loc: State of Confusion, Arkan... Flag
Last seen: 3 years, 10 months
Re: Dmt to raise psilocybin content? [Re: krypto2000]
    #24244678 - 04/14/17 08:38 PM (7 years, 1 month ago)

Nope, the mushies will only absorb so much of the DMT, and can only convert it during the early stages fruiting phase. In short it's a waste. Another way of making potent mushrooms is simply making the mycilium have to fight to stay alive. In response to stress they will make more alkaloids.


--------------------
"Love one another." "To Love is to know me." "Love is the Law, Love under Will." "In Compassion, all sorrows end." Regardless of the Master, the message is the same- Choose love and you shall live, Choose Fear and you shall die. Help bring peace to this Earth: Love one another, and serve others before yourself.

Extras: Filter Print Post Top
Jump to top Pages: 1 | 2 | 3  [ show all ]

Shop: OlympusMyco.com Olympus Myco Bulk Substrate   Bridgetown Botanicals Bridgetown Botanicals   Original Sensible Seeds Autoflowering Cannabis Seeds   Kraken Kratom Red Vein Kratom   North Spore Bulk Substrate   PhytoExtractum Buy Bali Kratom Powder   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Mono Tub Substrate   Myyco.com Golden Teacher Liquid Culture For Sale   MagicBag.co All-In-One Bags That Don't Suck


Similar ThreadsPosterViewsRepliesLast post
* Re: tryptamine, l,5-hydroxytryptophan (5-HTP) to increase psilocybin content Anonymous 4,160 8 02/24/00 10:22 PM
by Anonymous
* Lets start fresh...DMT substrate
( 1 2 3 4 5 all )
Bleuboxo 26,328 98 05/18/06 06:48 AM
by nimmen
* DMT/psilocybin increase detection gray1 1,229 2 06/05/01 07:48 PM
by jonnyshaggs420
* For doubters of the DMT substrate method.
( 1 2 all )
Explorer 9,264 30 05/22/01 09:10 AM
by gray1
* Re: Ayahuasca Substrate
( 1 2 3 all )
Anonymous 17,231 52 10/01/00 10:28 AM
by Anonymous
* vitamins and substrate cdlove69 4,205 19 03/13/02 06:38 PM
by triptamine
* effect of phosphorus in substrate........................... ph_plus 3,089 6 07/12/03 01:10 AM
by micro
* My shrooms grown on DMT-rich substrate. Dogomush 6,962 19 04/14/03 08:06 PM
by Catalysis

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, Pastywhyte, bodhisatta
3,473 topic views. 1 members, 3 guests and 2 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.037 seconds spending 0.005 seconds on 12 queries.