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InvisibleBsdgaou

Registered: 02/20/19
Posts: 340
Re: LC lid with polyfill only? [Re: Tira]
    #27309248 - 05/15/21 02:00 AM (3 years, 7 months ago)

Quote:

Tira said:
So I've finally bought some syringe filters and put them on lids using silicone.

and I'm following TL's ELME tek, which uses a gram of LME for 600 ml of water, which I did, and divided into three 400ml jars.



So what do you think, am I good to go?


I'm going to inoculate them with agar wedges and was wondering if I could just turn them upside down and stick the needle into the syringe filter and suck some LC when they're colonized?  Do syringe filters work like that?

and as I'm planning on building a magnetic stirrer with a computer fan and a magnet, would it work if I drop some screws in there (guess they could rust and I can find a better material) or should I just shake them as I already have syringe filters on them?




Can those lids with filter (on pictures) be re-used? Just pour new mix in jar, sterilise and make new strain LC? Or do I need make new ones every time?

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OfflineDERRAYLD
Constructus
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Registered: 05/13/02
Posts: 12,109
Loc: South Africa
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Re: LC lid with polyfill only? [Re: Bsdgaou]
    #27309261 - 05/15/21 02:38 AM (3 years, 7 months ago)

They're reusable

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InvisibleBsdgaou

Registered: 02/20/19
Posts: 340
Re: LC lid with polyfill only? [Re: DERRAYLD]
    #27309263 - 05/15/21 02:49 AM (3 years, 7 months ago)

I'm asking because they can get wet.

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