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Amazon Shop: ½ Pint Jars, Wild Bird Seed

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InvisibleMOTH
Wild Woman
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Registered: 06/06/03
Posts: 23,391
Loc: In the jungle
There was cobweb in my WBS!! Question...
    #2407208 - 03/08/04 03:11 PM (14 years, 3 months ago)

The other day I checked my 5 day old quart WBS jars, and noticed cobweb.  There was no mistaken it.  It looked EXACTLY like the pictures I've scene.  Gray and wispy and just not mycelium.  When I tossed the contents of the jar, a really sickly-sweet (almost overpowering) smell wafted up at me.  yech..

I know I sterilized these jars well, so for the future what can I do to prevent the formation of cobweb in my WBS jars?

This is my first attempt at WBS so I'm still learning.  :tongue:

Thanks!


*me*


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InvisibleCoolBlue
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Registered: 09/16/03
Posts: 619
Re: There was cobweb in my WBS!! Question... [Re: MOTH]
    #2408257 - 03/08/04 07:27 PM (14 years, 3 months ago)

cobweb hates oxygen and h202 so its easy to control once its on a casing

as for in the jars I would bleach your incubator and allow your wbs to sit for 24 hours before pcing for 75 minutes :laugh: that should solve your problem

also what type of filters are u using?


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OfflineSuntzu
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Registered: 10/15/99
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Re: There was cobweb in my WBS!! Question... [Re: MOTH]
    #2408380 - 03/08/04 07:58 PM (14 years, 3 months ago)

One often overlooked step is the cooling stage. . .let the PC cool from 15 PSI in as clean of an area as possible. Drap moist material over the vent as it cools. I prefer to leave the stopcock on until the unit reaches 2-3 PSI, then remove the stopcock and cover. This stops the rapid boiling that occurs if you let the thing freely vent [and doesn't make your whole room smell funny.] In any case, once jars have equilibrated, and assuming a decent filter is employed, it's rare to get airborne contamination. Bacillus, perhaps, but molds are not likely to survive PC'ing. More often they are introduced during the cooling or innoculation step.


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Amazon Shop: ½ Pint Jars, Wild Bird Seed

Mushrooms, Mycology and Psychedelics >> Contamination Forum

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