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Mateja


Registered: 07/14/16
Posts: 7,948
Loc: Here
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I cut off a portion of the swab and streak the he'll out of it on the plate and sometimes I even leave a piece of the cotton on the dish (I also had a hard time germinating from swabs)
-------------------- Cakes inside Water Tub
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Zero Nowhere
Beer Drinker



Registered: 01/29/18
Posts: 1,471
Loc: Standing on the moon
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That's what I did. I streaked 2 plates. I didn't know if I can keep the swab now for later or not.?
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Mateja


Registered: 07/14/16
Posts: 7,948
Loc: Here
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You probably shouldn't put the cotton onto nutrient rich media if you plan on using it again :/
That's why I cut off a portion of the cotton or throw away the swab after its spent.
-------------------- Cakes inside Water Tub
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Zero Nowhere
Beer Drinker



Registered: 01/29/18
Posts: 1,471
Loc: Standing on the moon
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Re: Making shoeboxes [Re: Mateja]
#25577035 - 10/29/18 06:25 PM (5 years, 3 months ago) |
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Yeah I kind of figured that. I reluctantly threw it away. I rubbed the shit out of it. Hopefully it takes. I'll try cutting it next time. How do you streak it after you cut it off?
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mushroomnate
Pstranger



Registered: 05/17/17
Posts: 3,100
Last seen: 2 days, 13 hours
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I was reading HERE fishermansjc digs a little divot into the agar and cuts & drops the piece into the divot. I may go ahead and try the cut method. Zero,let me know if those don't work out.
Edited by mushroomnate (10/29/18 06:41 PM)
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Mateja


Registered: 07/14/16
Posts: 7,948
Loc: Here
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I move it around with the loop or with a scalpel. Loop is much easier.
-------------------- Cakes inside Water Tub
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Zero Nowhere
Beer Drinker



Registered: 01/29/18
Posts: 1,471
Loc: Standing on the moon
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Re: Making shoeboxes [Re: Mateja]
#25577084 - 10/29/18 06:46 PM (5 years, 3 months ago) |
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I appreciate that nate! Yeah I read fisherman's tek before. Hopefully these ain't stubborn.
Thanks for the tip mateah.
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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Why don't y'all just flame sterilize a loop and swipe the damn swab? No cutting, no dropping, nothing.
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Zero Nowhere
Beer Drinker



Registered: 01/29/18
Posts: 1,471
Loc: Standing on the moon
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That's a good idea man. Now I really feel like a tard.
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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I just swipe the swab and save for later
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Asura
Cyantist


Registered: 08/01/11
Posts: 5,047
Loc: Texas
Last seen: 12 days, 3 hours
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Re: Making shoeboxes [Re: mushboy]
#25577324 - 10/29/18 08:27 PM (5 years, 3 months ago) |
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Quote:
mushboy said: I just swipe the swab and save for later
I'm really drunk
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Germs
Space Force


Registered: 06/26/11
Posts: 4,607
Loc: Texas
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Re: Making shoeboxes [Re: Asura] 1
#25577356 - 10/29/18 08:46 PM (5 years, 3 months ago) |
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Quote:
Asura said:
Quote:
mushboy said: I just swipe the swab and save for later
I'm really drunk
Hell yeah I just knocked up 10 qts with APE
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Zero Nowhere
Beer Drinker



Registered: 01/29/18
Posts: 1,471
Loc: Standing on the moon
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Re: Making shoeboxes [Re: Germs]
#25577359 - 10/29/18 08:48 PM (5 years, 3 months ago) |
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Mateja


Registered: 07/14/16
Posts: 7,948
Loc: Here
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Quote:
stareatclouds said: Why don't y'all just flame sterilize a loop and swipe the damn swab? No cutting, no dropping, nothing.
I haven't flamed anything in like a year or so lol I have a bunch of scalpels and loops so I just sterilise them all together with the other 15min PC items. Never needed more than 7-8 scalpels per SAB session
-------------------- Cakes inside Water Tub
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Zero Nowhere
Beer Drinker



Registered: 01/29/18
Posts: 1,471
Loc: Standing on the moon
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Re: Making shoeboxes [Re: mushboy]
#25577364 - 10/29/18 08:51 PM (5 years, 3 months ago) |
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Quote:
mushboy said: I just swipe the swab and save for later
Now ya tell me, man 
Edit: asura try not to make any shameful drunkin posts. That's how I always fuck up!
Edited by Zero Nowhere (10/29/18 08:59 PM)
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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Re: Making shoeboxes [Re: Mateja]
#25577392 - 10/29/18 09:01 PM (5 years, 3 months ago) |
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Quote:
Mateah said: I haven't flamed anything in like a year or so lol I have a bunch of scalpels and loops so I just sterilise them all together with the other 15min PC items. Never needed more than 7-8 scalpels per SAB session 
The standard X-acto knife can fit into the common thin, glass culture slants that RR has in his videos. I dropped 10 scalpels into them and sterilized them in quart jars, 5 blades each. Now I have fully sterile blades, including handle, to dig into my slants without fear of fucking them up.
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Brain Bulb
Insane in the Membrane



Registered: 11/09/17
Posts: 1,358
Last seen: 6 months, 24 days
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Just started a special shoebox of PCS from the wild prints I took this summer. I actually combined the culture from my print with the culture from a clone in the same jar. One was fluffy and the other looked like Doc’s hair from Back to the Future, lol. Since they are the same species they seemed to combine and colonized my GS in 6 days.

Check out my journal for more details and as always feedback is welcome!
All the best,
BB
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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that llooks like mold.
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Asura
Cyantist


Registered: 08/01/11
Posts: 5,047
Loc: Texas
Last seen: 12 days, 3 hours
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Quote:
Brain Bulb said: Just started a special shoebox of PCS from the wild prints I took this summer. I actually combined the culture from my print with the culture from a clone in the same jar. One was fluffy and the other looked like Doc’s hair from Back to the Future, lol. Since they are the same species they seemed to combine and colonized my GS in 6 days.

Check out my journal for more details and as always feedback is welcome!
All the best,
BB
My first 2 tropicalis plates looked like that. It's nasty. Poke that with a scalpel and you can feel it crunch. Throw those plates out.
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Brain Bulb
Insane in the Membrane



Registered: 11/09/17
Posts: 1,358
Last seen: 6 months, 24 days
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Re: Making shoeboxes [Re: Asura]
#25577626 - 10/29/18 10:30 PM (5 years, 3 months ago) |
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Quote:
Asura said:
Quote:
Brain Bulb said: Just started a special shoebox of PCS from the wild prints I took this summer. I actually combined the culture from my print with the culture from a clone in the same jar. One was fluffy and the other looked like Doc’s hair from Back to the Future, lol. Since they are the same species they seemed to combine and colonized my GS in 6 days.

Check out my journal for more details and as always feedback is welcome!
All the best,
BB
My first 2 tropicalis plates looked like that. It's nasty. Poke that with a scalpel and you can feel it crunch. Throw those plates out.
I’ve done two or three transfers with this and I haven’t heard any crunching. Doesn’t smell like mold either. Smell is very sweet like the Caerulescens fruit. Asked Bod a few days ago about it and was told it’s looking good. I’ve posted some more pics on his Q&A page but haven’t got a response yet.
There is a linear pattern on the surface of the agar it’s just hard to make out. Also if this was/is mold shouldn’t it have taken less time to colonize?
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