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Offlinemushroomnate
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Registered: 05/17/17
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Re: Making shoeboxes [Re: Failboat]
    #25430385 - 09/02/18 10:14 AM (5 years, 4 months ago)

This qt of oats is on 19 days after A2G.
Shaken once at about 30%.
Should be 100% by 21 days.

Started from this wedge drop.


Would you think this is a "normal" timeframe?


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BRF to bulk/Cakes as spawn


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InvisibleCaps McGee
Grandaddy Smurfshack
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Registered: 10/28/17
Posts: 14,357
Loc: ally known as ... Flag
Re: Making shoeboxes [Re: mushroomnate]
    #25430403 - 09/02/18 10:26 AM (5 years, 4 months ago)

Seems slow... and judging by the spots with no colonization, I'd suspect bacterial infection to be the issue... Might still do it's thing


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OfflineMartianman420
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Registered: 01/11/17
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Re: Making shoeboxes [Re: MadHatter333]
    #25430409 - 09/02/18 10:28 AM (5 years, 4 months ago)

Quote:

MadHatter333 said:
I was reading that the first spores landed on earth before the dinosaurs. Aliens from some far away planet jizzed on this land and that’s how mushrooms were born. Spores are just alien jizz that continues the life cycle over and over again for many years. At least that’s what I read in the magazine, Alien Insider.



:rofl:


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Thinking is not an option


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OfflineAsuraS
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Re: Making shoeboxes [Re: Caps McGee]
    #25430423 - 09/02/18 10:30 AM (5 years, 4 months ago)

Quote:

Caps McGee said:
A2G taking a month? :justno: something ain't right... more than 2 weeks and I'm tossing it




All my A2G takes a month. That's why I am going back to LC/LI. Even with several wedges, it
takes forever for the myc to move off the wedge. And my agar is made very weak, so it's not
like it's rich in nutes.


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InvisibleMycolorado
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Re: Making shoeboxes [Re: Asura]
    #25430435 - 09/02/18 10:34 AM (5 years, 4 months ago)

Whole oats take longer than other grains.


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InvisibleCaps McGee
Grandaddy Smurfshack
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Re: Making shoeboxes [Re: Mycolorado]
    #25430439 - 09/02/18 10:37 AM (5 years, 4 months ago)

Wow... I must have super ciltures


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OfflineFailboat
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Re: Making shoeboxes [Re: Caps McGee]
    #25430465 - 09/02/18 10:48 AM (5 years, 4 months ago)

Quote:

mushroomnate said:
This qt of oats is on 19 days after A2G.
Shaken once at about 30%.
Should be 100% by 21 days.

Started from this wedge drop.


Would you think this is a "normal" timeframe?



Quote:

Caps McGee said:
Seems slow... and judging by the spots with no colonization, I'd suspect bacterial infection to be the issue... Might still do it's thing



Looks fine to me, shoukd work out. I've used worse.


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Offlinemushroomnate
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Re: Making shoeboxes [Re: Failboat]
    #25430482 - 09/02/18 10:56 AM (5 years, 4 months ago)

These were from T1 Ape clone wedges,so could possibly be slightly bacterial.:shrug:
Got some other qts that I did with T3 multi-wedge drops,that look like they are gonna finish alot sooner.(multiple wedges=multiple inoculaton points)
Taking notes on all the advice.:takingnotes:


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InvisibleCaps McGee
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Registered: 10/28/17
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Re: Making shoeboxes [Re: mushroomnate]
    #25430498 - 09/02/18 11:04 AM (5 years, 4 months ago)

Did you shake it good? The growth doesn't look bad, it's the spots with no growth that concern me... but not super sweaty, or visible metabolites... I don't suspect it a lost cause


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InvisibleJohnny.rotten
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Registered: 08/16/18
Posts: 159
Re: Making shoeboxes [Re: Asura]
    #25430501 - 09/02/18 11:04 AM (5 years, 4 months ago)

Quote:

Asura said:
Quote:

Caps McGee said:
A2G taking a month? :justno: something ain't right... more than 2 weeks and I'm tossing it




All my A2G takes a month. That's why I am going back to LC/LI. Even with several wedges, it
takes forever for the myc to move off the wedge. And my agar is made very weak, so it's not
like it's rich in nutes.



I dropped whole agar pucks to 12 jars. I can't imagine it taking a month. From spore syringe, a month seems about right.


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Offlinemushroomnate
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Re: Making shoeboxes [Re: Caps McGee]
    #25430530 - 09/02/18 11:20 AM (5 years, 4 months ago)

Those spots have been filling in over the past few days.
4 days after 30% shake(middle jar)

7 days after shake(left jar)

9 days after shake(today)

Maybe I just waited too long to shake?:shrug2:
I have other batches that I may try 2 or multi shakes,too see if it makes a big difference.


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OfflineRomeoPapa
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Re: Making shoeboxes [Re: mushroomnate]
    #25430558 - 09/02/18 11:33 AM (5 years, 4 months ago)

2 to 3 weeks over here as well. A2G

My preferred method is the Tiger drop with a Pasty plate.


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It's better to have it and not need it
Than it is to need it and not have it.



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InvisibleJohnny.rotten
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Re: Making shoeboxes [Re: RomeoPapa]
    #25430565 - 09/02/18 11:37 AM (5 years, 4 months ago)

Quote:

RomeoPapa said:
2 to 3 weeks over here as well. A2G

My preferred method is the Tiger drop with a Pasty plate.



I tiger dropped 12 jars a few days ago. What do you average for colonization times using the whole pasty plates, as opposed to using just a wedge?


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OfflineRomeoPapa
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Re: Making shoeboxes [Re: Johnny.rotten]
    #25430590 - 09/02/18 11:49 AM (5 years, 4 months ago)

Idk if it's really all that much faster. Maybe a few days. I mainly Tiger drop because in my mind it's a safer way to inoculate jars.

I'm only opening the plate one time to drop it in the jar vs cutting wedges and opening the plate several times.

Also when a jar goes bad I dont have to worry about the rest of them as I would if I used one plate for several jars.

I also dont have to get out my torch and scalpel. Not like that's a big deal one way or the other.


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Than it is to need it and not have it.



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OfflineFailboat
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Re: Making shoeboxes [Re: RomeoPapa]
    #25430602 - 09/02/18 11:58 AM (5 years, 4 months ago)

Hell i just open my plate and cut a wedge toss it in a jar, pick up plate and xut a wedge, etc till finished


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Invisiblestareatclouds
star eat clouds?
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Registered: 09/29/14
Posts: 9,887
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Re: Making shoeboxes [Re: mushroomnate]
    #25430625 - 09/02/18 12:10 PM (5 years, 4 months ago)

Quote:

mushroomnate said:
This qt of oats is on 19 days after A2G.
Shaken once at about 30%.
Should be 100% by 21 days.

Started from this wedge drop.


Would you think this is a "normal" timeframe?




Looks like it's possibly bacterial. A second shake can speed things up. I personally would use an entire puck if I'm doing A2G. I also make sure the myc wedge faces the grains and not the glass.


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InvisibleCaps McGee
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Registered: 10/28/17
Posts: 14,357
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Re: Making shoeboxes [Re: stareatclouds]
    #25430652 - 09/02/18 12:24 PM (5 years, 4 months ago)

Shake the FUCK out of it after the drop... should spread even a  single wedge of growth around the jar, microscopic bits will colonize


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OfflineFailboat
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Re: Making shoeboxes [Re: stareatclouds]
    #25430657 - 09/02/18 12:26 PM (5 years, 4 months ago)

The idea behind the tiny but slow method is I minimize contamination vectors introduced into the sterilized jar. If a jar does get contaminated it is easily spotted early on and removed. The goal was to avoid the sneaky contams. I recently had a trich fest because a jar I G2G had a lil contam and after another round of G2G i had all kinds of trouble


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OfflineManifoldPrime
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Registered: 03/16/17
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Re: Making shoeboxes [Re: ManifoldPrime]
    #25430709 - 09/02/18 12:50 PM (5 years, 4 months ago)

Quote:

ManifoldPrime said:
Got some spawn I wanna make shoeboxes with, however some of the jars look kinda "wet" but I cant quite tell if its bacteria or not:shake:



anyone wanna chime in on what not to use?




Any chance anyone can take a quick look? I'm nervous about this batch since most of my boxes this year have triched out/stalled.


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OfflineFailboat
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Re: Making shoeboxes [Re: ManifoldPrime]
    #25430715 - 09/02/18 12:53 PM (5 years, 4 months ago)

What kind of filters you got on them?


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