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mushroomnate
Pstranger



Registered: 05/17/17
Posts: 3,100
Last seen: 2 days, 11 hours
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Re: Making shoeboxes [Re: Failboat]
#25430385 - 09/02/18 10:14 AM (5 years, 4 months ago) |
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This qt of oats is on 19 days after A2G. Shaken once at about 30%. Should be 100% by 21 days.
 Started from this wedge drop.

Would you think this is a "normal" timeframe?
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Caps McGee
Grandaddy Smurfshack



Registered: 10/28/17
Posts: 14,357
Loc: ally known as ...
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Seems slow... and judging by the spots with no colonization, I'd suspect bacterial infection to be the issue... Might still do it's thing
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Martianman420
Strangerdanger


Registered: 01/11/17
Posts: 1,043
Loc: Tejas
Last seen: 4 years, 8 months
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Quote:
MadHatter333 said: I was reading that the first spores landed on earth before the dinosaurs. Aliens from some far away planet jizzed on this land and thatβs how mushrooms were born. Spores are just alien jizz that continues the life cycle over and over again for many years. At least thatβs what I read in the magazine, Alien Insider.
-------------------- Thinking is not an option
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Asura
Cyantist


Registered: 08/01/11
Posts: 5,047
Loc: Texas
Last seen: 12 days, 45 minutes
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Quote:
Caps McGee said: A2G taking a month? something ain't right... more than 2 weeks and I'm tossing it
All my A2G takes a month. That's why I am going back to LC/LI. Even with several wedges, it takes forever for the myc to move off the wedge. And my agar is made very weak, so it's not like it's rich in nutes.
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Mycolorado
Hobbyist


Registered: 07/23/16
Posts: 8,529
Loc: Interdimensional Bootcamp
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Re: Making shoeboxes [Re: Asura]
#25430435 - 09/02/18 10:34 AM (5 years, 4 months ago) |
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Whole oats take longer than other grains.
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Caps McGee
Grandaddy Smurfshack



Registered: 10/28/17
Posts: 14,357
Loc: ally known as ...
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Wow... I must have super ciltures
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Failboat
Fuck Up

Registered: 02/01/18
Posts: 8,736
Last seen: 10 hours, 9 minutes
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Quote:
mushroomnate said: This qt of oats is on 19 days after A2G. Shaken once at about 30%. Should be 100% by 21 days.
 Started from this wedge drop.

Would you think this is a "normal" timeframe?
Quote:
Caps McGee said: Seems slow... and judging by the spots with no colonization, I'd suspect bacterial infection to be the issue... Might still do it's thing
Looks fine to me, shoukd work out. I've used worse.
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mushroomnate
Pstranger



Registered: 05/17/17
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Last seen: 2 days, 11 hours
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Re: Making shoeboxes [Re: Failboat]
#25430482 - 09/02/18 10:56 AM (5 years, 4 months ago) |
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These were from T1 Ape clone wedges,so could possibly be slightly bacterial. Got some other qts that I did with T3 multi-wedge drops,that look like they are gonna finish alot sooner.(multiple wedges=multiple inoculaton points) Taking notes on all the advice.
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Caps McGee
Grandaddy Smurfshack



Registered: 10/28/17
Posts: 14,357
Loc: ally known as ...
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Did you shake it good? The growth doesn't look bad, it's the spots with no growth that concern me... but not super sweaty, or visible metabolites... I don't suspect it a lost cause
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Johnny.rotten
Traveller


Registered: 08/16/18
Posts: 159
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Re: Making shoeboxes [Re: Asura]
#25430501 - 09/02/18 11:04 AM (5 years, 4 months ago) |
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Quote:
Asura said:
Quote:
Caps McGee said: A2G taking a month? something ain't right... more than 2 weeks and I'm tossing it
All my A2G takes a month. That's why I am going back to LC/LI. Even with several wedges, it takes forever for the myc to move off the wedge. And my agar is made very weak, so it's not like it's rich in nutes.
I dropped whole agar pucks to 12 jars. I can't imagine it taking a month. From spore syringe, a month seems about right.
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mushroomnate
Pstranger



Registered: 05/17/17
Posts: 3,100
Last seen: 2 days, 11 hours
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Those spots have been filling in over the past few days. 4 days after 30% shake(middle jar)
 7 days after shake(left jar)
 9 days after shake(today)
 Maybe I just waited too long to shake? I have other batches that I may try 2 or multi shakes,too see if it makes a big difference.
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RomeoPapa
Jackass of All Trades



Registered: 05/14/17
Posts: 1,583
Loc: In the middle
Last seen: 1 year, 2 months
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2 to 3 weeks over here as well. A2G
My preferred method is the Tiger drop with a Pasty plate.
-------------------- It's better to have it and not need it Than it is to need it and not have it.
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Johnny.rotten
Traveller


Registered: 08/16/18
Posts: 159
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Re: Making shoeboxes [Re: RomeoPapa]
#25430565 - 09/02/18 11:37 AM (5 years, 4 months ago) |
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Quote:
RomeoPapa said: 2 to 3 weeks over here as well. A2G
My preferred method is the Tiger drop with a Pasty plate.
I tiger dropped 12 jars a few days ago. What do you average for colonization times using the whole pasty plates, as opposed to using just a wedge?
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RomeoPapa
Jackass of All Trades



Registered: 05/14/17
Posts: 1,583
Loc: In the middle
Last seen: 1 year, 2 months
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Idk if it's really all that much faster. Maybe a few days. I mainly Tiger drop because in my mind it's a safer way to inoculate jars.
I'm only opening the plate one time to drop it in the jar vs cutting wedges and opening the plate several times.
Also when a jar goes bad I dont have to worry about the rest of them as I would if I used one plate for several jars.
I also dont have to get out my torch and scalpel. Not like that's a big deal one way or the other.
-------------------- It's better to have it and not need it Than it is to need it and not have it.
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Failboat
Fuck Up

Registered: 02/01/18
Posts: 8,736
Last seen: 10 hours, 9 minutes
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Re: Making shoeboxes [Re: RomeoPapa]
#25430602 - 09/02/18 11:58 AM (5 years, 4 months ago) |
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Hell i just open my plate and cut a wedge toss it in a jar, pick up plate and xut a wedge, etc till finished
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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Quote:
mushroomnate said: This qt of oats is on 19 days after A2G. Shaken once at about 30%. Should be 100% by 21 days.
 Started from this wedge drop.

Would you think this is a "normal" timeframe?
Looks like it's possibly bacterial. A second shake can speed things up. I personally would use an entire puck if I'm doing A2G. I also make sure the myc wedge faces the grains and not the glass.
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Caps McGee
Grandaddy Smurfshack



Registered: 10/28/17
Posts: 14,357
Loc: ally known as ...
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Shake the FUCK out of it after the drop... should spread even a single wedge of growth around the jar, microscopic bits will colonize
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Failboat
Fuck Up

Registered: 02/01/18
Posts: 8,736
Last seen: 10 hours, 9 minutes
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The idea behind the tiny but slow method is I minimize contamination vectors introduced into the sterilized jar. If a jar does get contaminated it is easily spotted early on and removed. The goal was to avoid the sneaky contams. I recently had a trich fest because a jar I G2G had a lil contam and after another round of G2G i had all kinds of trouble
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ManifoldPrime
Per Ardua Ad Astra



Registered: 03/16/17
Posts: 1,313
Loc: South Africa
Last seen: 1 year, 1 month
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Quote:
ManifoldPrime said: Got some spawn I wanna make shoeboxes with, however some of the jars look kinda "wet" but I cant quite tell if its bacteria or not

anyone wanna chime in on what not to use?
Any chance anyone can take a quick look? I'm nervous about this batch since most of my boxes this year have triched out/stalled.
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Failboat
Fuck Up

Registered: 02/01/18
Posts: 8,736
Last seen: 10 hours, 9 minutes
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What kind of filters you got on them?
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