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OfflineSutty86
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Re: Making shoeboxes [Re: mushboy]
    #25045826 - 03/07/18 12:36 PM (5 years, 10 months ago)

First attempt at a shoebox
Haven't got a ratio,
One jar of spawn less than a quart(uk member)
And some cvg again just chucked in with boiling water
Think I'm getting my first pins


--------------------
Looking for PENIS ENVY.
Have spores to trade:mushroom2:


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OfflineShrooms4life
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Re: Making shoeboxes [Re: Sutty86]
    #25045849 - 03/07/18 12:48 PM (5 years, 10 months ago)

Quote:

Sutty86 said:
First attempt at a shoebox
Haven't got a ratio,
One jar of spawn less than a quart(uk member)
And some cvg again just chucked in with boiling water
Think I'm getting my first pins




Did you case that?


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InvisiblemushboyMDiscord
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Re: Making shoeboxes [Re: Shrooms4life]
    #25045859 - 03/07/18 12:53 PM (5 years, 10 months ago)

i either see lucy style pins or mold hahah what variety is that??


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OfflineSutty86
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Re: Making shoeboxes [Re: mushboy]
    #25045867 - 03/07/18 12:56 PM (5 years, 10 months ago)

AA+


--------------------
Looking for PENIS ENVY.
Have spores to trade:mushroom2:


Edited by Sutty86 (03/07/18 01:01 PM)


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InvisiblemushboyMDiscord
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Re: Making shoeboxes [Re: Sutty86]
    #25045873 - 03/07/18 12:57 PM (5 years, 10 months ago)

looks good for your first job. just tossed some shit together in a tote:thumbup:
:nodofunderstanding:

or its trich. sometimes those white pins show up weird on pics like trich does pre sporulation.


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OfflineSutty86
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Re: Making shoeboxes [Re: mushboy]
    #25045890 - 03/07/18 01:03 PM (5 years, 10 months ago)

Quote:

mushboy said:
looks good for your first job. just tossed some shit together in a tote:thumbup:
:nodofunderstanding:

or its trich. sometimes those white pins show up weird on pics like trich does pre sporulation.



Fuck sake, will update tomorrow.
Better not he trich


--------------------
Looking for PENIS ENVY.
Have spores to trade:mushroom2:


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InvisiblemushboyMDiscord
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Re: Making shoeboxes [Re: Sutty86]
    #25045897 - 03/07/18 01:07 PM (5 years, 10 months ago)

haha i dont think it is. looks like aa+ knots/pins similar to mine


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OfflineSpivkurl
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Re: Making shoeboxes [Re: mushboy]
    #25045991 - 03/07/18 01:55 PM (5 years, 10 months ago)

Yep, if great white monster is indeed the same as AA+, then it looks much like mine do in early knotting moments.


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InvisiblePsicomb
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Re: Making shoeboxes [Re: Sutty86]
    #25046049 - 03/07/18 02:16 PM (5 years, 10 months ago)

Quote:

Sutty86 said:
Quote:

mushboy said:
looks good for your first job. just tossed some shit together in a tote:thumbup:
:nodofunderstanding:

or its trich. sometimes those white pins show up weird on pics like trich does pre sporulation.



Fuck sake, will update tomorrow.
Better not he trich




Yeah those look like my AA+ knots and pins  

I bet that'll take off by tomorrow eve.


--------------------

When we constantly pull things apart trying to see how it works, we may end up with only an understanding of how to destroy something
- nick sand


Edited by Psicomb (03/07/18 02:19 PM)


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OfflineFailboat
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Re: Making shoeboxes [Re: Steevo]
    #25046237 - 03/07/18 02:58 PM (5 years, 10 months ago)

Quote:

Steevo said:
Quote:

Quirkmeister92 said:
Quote:

Steevo said:
Colonized enough? Either fully colonized or nothing imo. Why would you open a jar or bag of spawn that wasn't 100%
:waitwtf:



Duh, I was asking for your opinion as to if you consider that 100% or not. So if you would like to share...




Wet grains pushed up against the glass that never fully colonize are a bad sign. I’d spawn all the jars that look like that to separate shoeboxes and cross my fingers they give at least 1 flush before crapping out. Its hard to get a good look at what’s going on without really clear pics but that’s just my opinion and wtf do I know anyway?



Thanks, seems like you know enough to say that much, which reaffirms my thought to wait. I was just getting trigger happy. I shook em and they were far from 100, and I managed to unstick much if not all from thw sides in most of those jars. Thank you


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Offlineproustdreams
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Re: Making shoeboxes [Re: Failboat]
    #25046661 - 03/07/18 04:34 PM (5 years, 10 months ago)

Getting ready to start agar for my next shoebox endeavor.  I'll hopefully get a half dozen nice agar cultures to test out in shoeboxes. 

Few questions

1.  I'll be going from spore print --> agar via sterile swab.  I'll presumably do a few transfers until I get some pretty and uniform mycelium.  Is this final agar dish (that I'll be doing agar to grain with) going to yield the same genetics/strain for all the fruits?  I'm not sure how to word this and I'm sure I'm butchering terminology, but will all the fruits essentially be "clones" of the agar donor?  In other words, if I were to take a live tissue clone of the fruit, will it be the same genetics as the original agar inoculant. 

2.  Most (if not all) of the PE grow logs I've been following use at least a CVG pseudo casing, or true casing.  Is there any reason only coir casing wouldn't work as a PE pseudo casing? 



Edited by proustdreams (03/07/18 04:39 PM)


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OfflineFailboat
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Re: Making shoeboxes [Re: proustdreams] * 1
    #25046906 - 03/07/18 05:49 PM (5 years, 10 months ago)

Spawned ~30hrs ago. 1:~2 (2xBrown Rice), (red Spring Berries, Millet, Grass Seed)






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InvisiblemushboyMDiscord
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Re: Making shoeboxes [Re: Failboat]
    #25046974 - 03/07/18 06:07 PM (5 years, 10 months ago)

ccol nice containers.


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OfflineFailboat
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Re: Making shoeboxes [Re: mushboy]
    #25046988 - 03/07/18 06:13 PM (5 years, 10 months ago)

Found em at mendards. 6pk for $5, so I snagged like 36 or so


Edited by Failboat (03/07/18 06:14 PM)


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Offlinedmppb
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Re: Making shoeboxes [Re: Humble Newcomer]
    #25047285 - 03/07/18 08:09 PM (5 years, 10 months ago)

Quote:

Humble Newcomer said:
Oh man there was more pages wtf.

That was in response to quirkmasters tub. To achieve uniformity.

Mushboy was talking about senescence, If u keep cloning a clone it will eventually get old and die. More likely it won't die but will fruit poorly and be contam prone.

When u have a good grow and want to clone that mushroom think of it as chopping off the arm of your favorite mush guy, and then your mushroom regrows its arm but the arm also regrows it's whole body. That's the stupid way I think of it. You don't grow a kid from an arm, u regrow the donor body.

You can do this over and over. And each one will be an exact duplicate because it is in fact the same organism.

But after a grow u take a mush and clone it on agar, now u put wedges to grains, grains to coir, wait, fruit, clone, back to agar back to grains... But all with the same old tired specimen. It will eventually stop performing well. Basically the same old guy doing grow after grow. And the organism is aging in real time a month or two each grow. Eventually he will produce less and less.

At that point u would have to take a spore print from your best performer.

That will be a parent to a new generation of kids (spores) and if the parent was a desired specimen like it was big well all the kids have a chance of being big too.

But smart human parents have stupid kids sometimes and red hair skips generations doesn't it, same with shrooms, so a big fat fruit will have millions of spores but not all will grow to be big. That's why we want to clone and maintain a culture when it's good and proven, to keep it.

So what's best is to have a master slants or similar to maintain your culture without it aging. You can keep it for life because it only comes out of stasis long enough for u to get a wedge out and put on a dish. Put master back up in fridge. It slows time way down, a year in the fridge may the same as two weeks at room temp or whatever. Now Let grow plate grow out and u get 30+ wedges from it for 30+ grain masters which makes 300+ quarts.

All from one wedge of the master which never aged bc it stays locked up in stasis

Edit - it seems many active growers don't bother with it, and I can see why, but in gourmet I have 3 or 4 different kinds of oysters and 2 shiitake already and I just started. Each oyster is different color and requires diff fruiting temps so they're seasonal and beautiful and $20/lb retail at farmers market and basically maintaining cultures becomes key. It's impossible to keep redoing each culture looking for good performers




great explaining on how to get good cultures cultures , genetics do a lot of the work


--------------------
...and when you loose control ; you'll reap the harvest you have sown.


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InvisibleMunchauzen
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Re: Making shoeboxes [Re: Humble Newcomer]
    #25047302 - 03/07/18 08:17 PM (5 years, 10 months ago)

Quote:

Humble Newcomer said:
Also to quirkmasters, full colonization plus one week before spawning.




no need to wait a week. thats only for pf-tek. grains you should spawn ASAP when they are 100%. there is no benefit to consolidate them.


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OfflineTheBeez
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Re: Making shoeboxes [Re: Munchauzen]
    #25047451 - 03/07/18 09:24 PM (5 years, 10 months ago)

Quote:

Munchauzen said:
Quote:

Humble Newcomer said:
Also to quirkmasters, full colonization plus one week before spawning.




no need to wait a week. thats only for pf-tek. grains you should spawn ASAP when they are 100%. there is no benefit to consolidate them.





Is this the case for PE as well?


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OfflineShrooms4life
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Re: Making shoeboxes [Re: TheBeez]
    #25047457 - 03/07/18 09:28 PM (5 years, 10 months ago)

That's the case with all grain spawn, no consolidation period like brf cakes.


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InvisibleJust_A_Noob
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Re: Making shoeboxes [Re: Shrooms4life]
    #25047462 - 03/07/18 09:31 PM (5 years, 10 months ago)



--------------------

Wearing a mask is bad for my physical, emotional, and spiritual health.
Complying = Consent
Wide Mouth 1/2 Pint No-Pour TEK
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OfflineFailboat
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Re: Making shoeboxes [Re: Munchauzen]
    #25047540 - 03/07/18 10:26 PM (5 years, 10 months ago)

Quote:

Munchauzen said:
Quote:

Humble Newcomer said:
Also to quirkmasters, full colonization plus one week before spawning.




no need to wait a week. thats only for pf-tek. grains you should spawn ASAP when they are 100%. there is no benefit to consolidate them.



Right on, that's what ai understood to be the case. As for the difference between mostly colonized and 100% it becomes hazy. I have some ultra thick growth across some pint jars from MS, but some jars with less thick growth, like in thw pics previously posted where all of the grains have myc, but no so much as that thd grain is thickly inundated.


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